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提高产抗生素链霉菌紫外诱变正变率的研究 被引量:8
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作者 吴雪昌 汪志芸 +2 位作者 周婕 朱旭芬 钱凯先 《遗传》 CAS CSCD 北大核心 2004年第4期499-504,共6页
将UV诱变了的产抗生素链霉菌(Streptomycessp.)AP19 1菌株之孢子,置于适宜的生长温度27℃与接近抑制生长的胁迫温度33℃下培养,结果表明:在33℃下生长获得的子代菌株中,产抗生素水平超过其出发菌株的正向突变体所占的比例,明显比在27℃... 将UV诱变了的产抗生素链霉菌(Streptomycessp.)AP19 1菌株之孢子,置于适宜的生长温度27℃与接近抑制生长的胁迫温度33℃下培养,结果表明:在33℃下生长获得的子代菌株中,产抗生素水平超过其出发菌株的正向突变体所占的比例,明显比在27℃下培养的高。27℃下培养,正向突变体占总子代菌株数的25.8%,而在33℃下培养则为58.1%。用17种随机引物对出发菌株与UV诱变子代菌株进行总DNA的RAPD测验证明,在接近抑制生长的温度33℃下培养获得的子代,发生在其DNA水平上的变异程度比在27℃的要高得多。这一方法能较大幅度提高链霉菌紫外诱变育种的工作效率,同时也为链霉菌经紫外诱变后突变形成机制的进一步研究提供了新途径。 展开更多
关键词 紫外诱变 链霉菌 正突变体 RAPD
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秀丽隐杆线虫抗铜突变体的筛选及SNP定位
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作者 宋少娟 郭亚平 +2 位作者 张学尧 张建珍 马恩波 《遗传》 CAS CSCD 北大核心 2014年第12期1261-1268,共8页
铜在有机体代谢过程中发挥着重要作用,但过量可产生毒害效应。文章以秀丽隐杆线虫(Caenorhabditis elegans)为模式生物,寻找多细胞生物中铜代谢调节的关键基因。采用甲基磺酸乙酯(EMS)诱变秀丽隐杆线虫,通过100 000个杂合基因组的筛选... 铜在有机体代谢过程中发挥着重要作用,但过量可产生毒害效应。文章以秀丽隐杆线虫(Caenorhabditis elegans)为模式生物,寻找多细胞生物中铜代谢调节的关键基因。采用甲基磺酸乙酯(EMS)诱变秀丽隐杆线虫,通过100 000个杂合基因组的筛选得到两个抗铜突变体ms1和ms2。在筛选培养基上野生型停止发育,而抗铜突变体则可发育到成虫,且抗铜性状能稳定遗传。与N2的回交实验表明,ms1的抗铜表型可能由单基因隐性突变导致,ms2的抗铜表型消失,可能是由多基因突变引起。以CB4856和ms1作为亲本,构建了F2群,经SNP定位,确定ms1突变位点位于染色体II(LGII)上,进一步对LGII染色体上的8个SNP标记进行分析,将ms1的突变位点定位在LGII:-6附近。秀丽隐杆线虫抗铜突变体ms1的筛选和定位可为深入研究线虫铜代谢及调控的分子机制提供实验依据。 展开更多
关键词 秀丽隐杆线虫 正向遗传筛选 抗铜 突变体 SNP定位
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酵母紫外线诱变后培养温度与酒精发酵性状变异相关性研究 被引量:3
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作者 戴朝波 吴雪昌 严力蛟 《科技通报》 2008年第3期325-329,共5页
酿酒酵母(Saccharomyces cerevisiae)Hs27α菌株经UV诱变后,分别置于31℃、40℃等不同的温度下培养,结果表明:在胁迫温度40℃下培养,子代菌株中正突变菌株(指产酒量高于对照的菌株)的平均产酒能力明显高于31℃下培养的菌株。31℃下培养... 酿酒酵母(Saccharomyces cerevisiae)Hs27α菌株经UV诱变后,分别置于31℃、40℃等不同的温度下培养,结果表明:在胁迫温度40℃下培养,子代菌株中正突变菌株(指产酒量高于对照的菌株)的平均产酒能力明显高于31℃下培养的菌株。31℃下培养的正突变菌株的平均产酒量提高4.89%,而在40℃下培养的则为8.75%。用15种随机引物对出发菌株与UV诱变子代菌株进行总DNA的RAPD测验证明,在接近抑制生长的胁迫温度(本文为40℃)下培养获得的子代中,DNA水平的变异程度比在31℃的要高得多。这一方法较大幅度提高了酵母紫外诱变选育高产菌种的效率,也为酿酒酵母UV诱变机制的进一步研究提供了新途径。 展开更多
关键词 紫外诱变 酿酒酵母 正突变体 RAPD
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Pigmentation Restored in Mutant Laboratory Strain of the Lady Beetle Coleomegilla maculata through Dietary Supplementation 被引量:1
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作者 Margaret Louise Allen 《Advances in Entomology》 2016年第3期133-140,共8页
A laboratory colony of Coleomegilla maculata (DeGeer), ye, selected for a pigmentation deficiency, was restored to near wild type cuticle coloration by adding crushed heads and wings of the red colored parental strain... A laboratory colony of Coleomegilla maculata (DeGeer), ye, selected for a pigmentation deficiency, was restored to near wild type cuticle coloration by adding crushed heads and wings of the red colored parental strain to the diet. While the wings and other colored portions of the cuticle re-gained the red color, the eyes of the pigmentation deficient insects were not changed from the pale mutant form. Plant derived carotenes lycopene and beta-carotene did not restore the mutant beetles to a visibly distinguishable red color. An additional pigmentation deficient mutant strain, gold, partially recovered red cuticle color when provided with diet containing pigmented insect particles. This work represents the first rescue of a color phenotype in a lady beetle. 展开更多
关键词 Lady Beetle Recessive Phenotype Carotenoid Pigments Forward Genetics Mutant Rescue Dietary Supplement
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Pigmentation Restored in Mutant Laboratory Strain of the Lady Beetle <i>Coleomegilla maculata</i>through Dietary Supplementation
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作者 Margaret Louise Allen 《Advances in Entomology》 2016年第3期133-140,共8页
A laboratory colony of <i>Coleomegilla maculata</i> (DeGeer), ye, selected for a pigmentation deficiency, was restored to near wild type cuticle coloration by adding crushed heads and wings of the red colo... A laboratory colony of <i>Coleomegilla maculata</i> (DeGeer), ye, selected for a pigmentation deficiency, was restored to near wild type cuticle coloration by adding crushed heads and wings of the red colored parental strain to the diet. While the wings and other colored portions of the cuticle re-gained the red color, the eyes of the pigmentation deficient insects were not changed from the pale mutant form. Plant derived carotenes lycopene and beta-carotene did not restore the mutant beetles to a visibly distinguishable red color. An additional pigmentation deficient mutant strain, gold, partially recovered red cuticle color when provided with diet containing pigmented insect particles. This work represents the first rescue of a color phenotype in a lady beetle. 展开更多
关键词 Lady Beetle Recessive Phenotype Carotenoid Pigments Forward Genetics Mutant Rescue Dietary Supplement
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