Linkage analysis and integrated software GAPL for pure-line populations derived from four-way and eight-way crosses

Pure lines derived from multiple parents provide abundant variation for genetic study.However,efficient genetic analysis methods and user-friendly software are still lacking.In this study, we developed linkage analysis methods and integrated analysis software for pure-line populations derived from four-way and eight-way crosses.First, polymorphic markers are classified into different categories according to the number of identifiable alleles in the inbred parents.Expected genotypic probability is then derived for each pair of complete markers, and based on them a maximum likelihood estimate(MLE) of recombination frequency is calculated.An EM algorithm is proposed for calculating recombination frequencies in scenarios that at least one marker is incomplete.A linkage map can thus be constructed using estimated recombination frequencies.We describe a software package called GAPL for recombination frequency estimation and linkage map construction in multi-parental pure-line populations.Both simulation studies and results from a reported four-way cross recombinant inbred line population demonstrate that the proposed method and software can build more accurate linkage maps in shorter times than other published software packages.The GAPL software is freely available from www.isbreeding.net and can also be used for QTL mapping in multi-parental populations.

Simulation of semisolid diecasting process of four-way valve of HPb59-1 alloy for air-conditioner

There exist many problems in producing four-way valve of HPb59-1 alloy for an air-conditioner by traditional solid state hot forging,i.e.larger forming loads,lower material utilization,larger subsequent machining allowance and nonuniform microstructure.For this reason,based on the orthogonal test,the semisolid diecasting process of a certain type of four-way valve was simulated with FLOW-3D.The simulation results show that the optimized process parameters are:the pouring temperature of 897.25 ?C,the shot velocity of 2.0 m/s and the preheated die temperature of 260 C.The simulation results demonstrate that the cavity can be filled smoothly and completely,the surface defects are small,the temperature field and the pressure field are uniform,and the casting quality is satisfactory.The effectiveness and availability of applying this technology are well verified by the numerical simulation.

Identifying Genomic Regions Conferring Morphological and Yield-related Traits in Soybean Based On A Four-way Recombinant Inbred Line Population

Improvement of seed yield of soybean(Glycine max(L.)Merr.)is generally achieved by combining morphological and yield-related traits,such as plant height(PH),node number on main stem(NN),pod number per plant(NP),seed number per plant(NS),100-seed weight(HSW)and seed weight per plant(SWPP).Identifying quantitative trait loci(QTLs)for morphological and yield-related traits is therefore important for breeding.In this study,a four-way recombinant inbred line population comprising 160 lines derived from the cross(Kenfeng14×Kenfeng15)×(Heinong48×Kenfeng19)was planted in five different environments and morphological and yield-related trait data were used to identify QTLs by the inclusive composite interval mapping method.Totally 38 QTLs for PH,40 QTLs for NN,26 QTLs for NP,10 QTLs for NS,26 QTLs for HSW and 49 QTLs for SWPP were detected in 125 genomic regions.Single QTLs explained 2.17%-14.60%,2.00%-10.04%,2.37%-9.77%,2.62%-8.61%,0.47%-6.51%and 0.14%-12.39%of the phenotypic variation for PH,NN,NP,NS,HSW and SWPP,respectively.Among these 125 genomic regions,120 were newly associated with morphological and yield-related traits.The results would facilitate the molecular breeding of morphological and yield-related traits in soybean.

A novel four-way combining catalysts for simultaneous removal of exhaust pollutants from diesel engine

A novel four-way combining catalysts containing double layers was applied to simultaneously remove four kinds of exhaust pollutants （NOx, CO, HC and PM） emitted from diesel engine. The four-way catalysts were characterized using scanning electron microscope （SEM） and Ultraviolet visible diffuse reflectance spectroscopy （UV-Vis DRS）. Their catalytic performances were evaluated by temperature-programmed reaction technology. The double layer catalysts could effectively remove the four main pollutants. The highest catalytic activity was given by the two-layered catalysts of La0.6 K0.4CoO3/Al2O3 and W/HZSM-5. Under the simulated exhaust gases conditions, the peak temperature of the soot combustion was 421℃, the maximal conversion of NO to N2 was 74%, the temperature of the HC total conversion was 357℃, and the maximum conversion ratio of CO was 99%.

Exploration of the “Four-Three-Four” Internship and Training Mode in Higher Vocational Colleges Under the Modern Vocational Education System

This article mainly introduces the innovative internship and training model of vocational colleges,including five aspects such as“four-party collaboration,three-stage progression,four-way integration,value-added evaluation,and double selection and promotion.”The model aims to improve students’practical skills and professional quality to better adapt to market demand and social development.The article also presents the prospects of the future internship and training model,including strengthening cooperation and communication with industry enterprises,focusing on students’personalized development and practical skill cultivation,and establishing a scientific and objective evaluation and feedback mechanism.

Breeding of Healthy Fine Size Trimolter Variety Yuecanxixian No. 1

Taking introduced trimolter varieties 853 Bai and 543B with stable moltinism as male parents, and tetrarnolter varieties 932, 7532, Furong, Xianghui with excellent comprehensive economic characters in current production as female parents, the trimolter varieties San, Long, Hui and Yuan with excellent compre- hensive economic characters and stable mohinism were bred using cross breeding and systematic breeding methods, and healthy trimoher four-way cross combination ＂San · Long × Hui · Yuan＂ （ Yuecanxixian No. 1 ） was assembled. Provincial laboratory and rural production and identification results showed that the variety had strong vitality, stable moltinism （trimolt rate over 98% ） and short duration; laboratory identification results were as follows： cocoon filament size 1. 853 dtex, cocoon filament length 1 146 m, reelability percentage 73.04%, neatness 95.38 points, strength 4.63 mN/dtex, elongation rate 23.3%, cohesion 116 times. The variety was approved by Guangdong Crop Variety Approval Committee in December 2010, which can be used as raw cocoon for reeling high-grade fine size raw silk.

Effects of the inlet particle spatial distribution on the performance of a gas-solid cyclone separator

The present study was aimed at the inlet particle spatial distribution of a gas-solid separator with high solid loading and investigated its effects on cyclone performance and the inner flow pattern.The flow fields of a cyclone with different rectangular particle flow areas on the inlet surface were numerically simulated using a four-way coupling method.The simulated results indicate that reducing the inlet particle flow area and lowing the inlet particle position can effectively reduce the scattered particles inside the cyclone separator and enhance the separation performance.Vertically gathering the particles to the centerline can also weaken the particle back-mixing.The particles near the roof account for the swirling particle ceiling phenomenon.The inlet particle spatial distribution affects the pressure drop mainly by affecting the gas tangential velocity in the cylinder body.Moreover,compared to the hori-zontal particle distribution on the inlet surface,the vertical particle distribution has greater effects on cyclone performance.

Differentially weighted direct simulation Monte Carlo method for particle collision in gas-solid flows

In gas-solid flows, particle-particle interaction （typical, particle collision） is highly significant, despite the small particles fractional volume. Widely distributed polydisperse particle population is a typical characteristic during dynamic evolution of particles （e.g., agglomeration and fragmentation） in spite of their initial monodisperse particle distribution. The conventional direct simulation Monte Carlo （DSMC） method for particle collision tracks equally weighted simulation particles, which results in high statistical noise for particle fields if there are insufficient simulation particles in less-populated regions. In this study, a new differentially weighted DSMC （DW-DSMC） method for collisions of particles with different number weight is proposed within the framework of the general Eulerian-Lagrangian models for hydrodynamics. Three schemes （mass, momentum and energy conservation） were developed to restore the numbers of simulation particle while keeping total mass, momentum or energy of the whole system unchanged respectively. A limiting case of high-inertia particle flow was numerically simulated to validate the DW-DSMC method in terms of computational precision and efficiency. The momentum conservation scheme which leads to little fluctuation around the mass and energy of the whole system performed best. Improved resolution in particle fields and dynamic behavior could be attained simultaneously using DW-DSMC, compared with the equally weighted DSMC. Meanwhile, computational cost can be largely reduced in contrast with direct numerical simulation.

Intracellular Logic Computation with Framework Nucleic Acid-Based Circuits for mRNA Imaging

Main observation and conclusion DNA circuits have been designed for implementation of various functions based on DNA strand displacement in cell-free settings,but their capabilities in biological environments remain limited.In this work,we report framework nucleic acid(FNA)-based circuits enabling intracellular logic computation for mRNA imaging.FNAs as rigid scaffolds enable to deliver our built DNA circuits into cells without aid of transfection reagents,evading a time-consuming and tedious process prior to analysis,and the pendant duplex DNA designed at one vertex of FNA as gate is suitable for four-way strand exchange,minimizing crosstalk with other nucleic acids in the cellular milieu.We demonstrated that such FNA-based circuits can operate both in vitro and in vivo logic computation,including OR and AND logic gates.Moreover,in situ mRNA imaging was also realized by exploiting native mRNA as scaffolds to bind multiple FNA-based gates for the enhanced signal-to-background ratio.We hope that this FNA-based circuit can be applied for disease diagnosis,facilitating the development of biomedicine.