Low and high-concentration nitric wastewater can induce stable nitrite accumulation and realize the nitrification system, with the nitrite accumulation rate between 50% and 90%. In the low-concentration nitrite nitrif...Low and high-concentration nitric wastewater can induce stable nitrite accumulation and realize the nitrification system, with the nitrite accumulation rate between 50% and 90%. In the low-concentration nitrite nitrification system, the average FA concentrations during the period of stable nitrite accumulation remained at 7 to 10 mg/L. In the high-concentration nitrite nitrification system, the nitrogen oxidation activity will not be affected by biomass, the nitrite oxidation activity is related to the system biomass, it is recommended to be measured by FA/MLSS. Keeping the FA sludge load below 0.1 is the precondition of making good nitrite accumulation and nitrogen degradation rate. Nitrite oxidation bacteria cannot be totally eliminated or washed out from the system.展开更多
Nitrifying biomass on ring-shaped carriers was modified to nitritating one in a relatively short period of time （37 days） by limiting the air supply, changing the aeration regime, shortening the hydraulic retention ...Nitrifying biomass on ring-shaped carriers was modified to nitritating one in a relatively short period of time （37 days） by limiting the air supply, changing the aeration regime, shortening the hydraulic retention time and increasing free ammonia （FA） concentration in the moving-bed biofilm reactor （MBBR）. The most efficient strategy for the development and maintenance of nitritating biofilm was found to be the inhibition of nitrifying activity by higher FA concentrations （up to 6.5 mg/L） in the process. Reject water from sludge treatment from the Tallinn Wastewater Treatment Plant was used as substrate in the MBBR. The performance of high-surfaced biocarriers taken from the nitritating activity MBBR was further studied in batch tests to investigate nitritation and nitrification kinetics with various FA concentrations and temperatures. The maximum nitrite accumulation ratio （96.6%） expressed as the percentage of NO 2 ？ -N/NOx ？ -N was achieved for FA concentration of 70 mg/L at 36°C. Under the same conditions the specific nitrite oxidation rate achieved was 30 times lower than the specific nitrite formation rate. It was demonstrated that in the biofilm system, inhibition by FA combined with the optimization of the main control parameters is a good strategy to achieve nitritating activity and suppress nitrification.展开更多
Free ammonia（FA） inhibition on nitrite-oxidized bacteria（NOB） and real-time control are used to achieve nitrogen removal from landfill leachate via nitrite pathway at low temperatures in sequencing batch reactor. ...Free ammonia（FA） inhibition on nitrite-oxidized bacteria（NOB） and real-time control are used to achieve nitrogen removal from landfill leachate via nitrite pathway at low temperatures in sequencing batch reactor. The inhibition of FA on NOB activity during the aerobic period was prolonged using real-time control. The degree of nitrite accumulation was monitored along with variations of the ammonia-oxidizing bacteria and NOB population using fluorescence in situ hybridization techniques. It is demonstrated that the end-point of ammonia oxidization is detected from the on-line measured dissolved oxygen,oxidization–reduction potential, and p H signals, which could avoid the loss the FA inhibition on NOB caused by excess aeration. At low temperature（13.0–17.6°C）, the level of nitrite pathway rapidly increased from 19.8% to 90%, suggesting that nitritation was successfully started up at low temperature by applying syntrophic association of the FA inhibition and real-time control, and then this high level of nitrite pathway was stably maintained for as long as 233 days. Mechanism analysis shows that the establishment of nitritation was primarily the result of predominant ammonia-oxidizing bacteria developed in the nitrifying bacteria population compared to NOB. This was mainly due to a gradual reduction of nitrite amount that is available to provide energy for the growth of NOB,eventually leading to the elimination of NOB from the bacterial clusters in sequencing batch reactor sludge system.展开更多
Anaerobic acidogenic fermentation with high-solid sludge is a promising method for volatile fatty acid（VFA） production to realize resource recovery. In this study, to model inhibition by free ammonia in high-solid s...Anaerobic acidogenic fermentation with high-solid sludge is a promising method for volatile fatty acid（VFA） production to realize resource recovery. In this study, to model inhibition by free ammonia in high-solid sludge fermentation, the anaerobic digestion model No. 1（ADM1） was modified to simulate the VFA generation in batch, semicontinuous and full scale sludge. The ADM1 was operated on the platform AQUASIM 2.0.Three kinds of inhibition forms, e.g., simple inhibition, Monod and non-inhibition forms,were integrated into the ADM1 and tested with the real experimental data for batch and semi-continuous fermentation, respectively. The improved particle swarm optimization technique was used for kinetic parameter estimation using the software MATLAB 7.0. In the modified ADM1, the Ksof acetate is 0.025, the km,acis 12.51, and the KI_NH3is 0.02,respectively. The results showed that the simple inhibition model could simulate the VFA generation accurately while the Monod model was the better inhibition kinetics form in semi-continuous fermentation at pH 10.0. Finally, the modified ADM1 could successfully describe the VFA generation and ammonia accumulation in a 30 m^3full-scale sludge fermentation reactor, indicating that the developed model can be applicable in high-solid sludge anaerobic fermentation.展开更多
基金supported by Guizhou Science and Technology Fund Project 2008-2236the Scientific and Technological Project under Science and Technology Department of Guizhou Province [SZ(2008)3073]Guizhou University’s Scientific Research Personnel Introduction Pro-gram 2008-029
文摘Low and high-concentration nitric wastewater can induce stable nitrite accumulation and realize the nitrification system, with the nitrite accumulation rate between 50% and 90%. In the low-concentration nitrite nitrification system, the average FA concentrations during the period of stable nitrite accumulation remained at 7 to 10 mg/L. In the high-concentration nitrite nitrification system, the nitrogen oxidation activity will not be affected by biomass, the nitrite oxidation activity is related to the system biomass, it is recommended to be measured by FA/MLSS. Keeping the FA sludge load below 0.1 is the precondition of making good nitrite accumulation and nitrogen degradation rate. Nitrite oxidation bacteria cannot be totally eliminated or washed out from the system.
基金supported by the Estonian Environmental Investment Center Program "Treatment of Nitrogen- rich Wastewaters (SLOTI08262)"a target-financed project of the Estonian Ministry of Education and Research NoSF0180135s08, named "Processes in macro-and microheterogeneous and nanoscale systems and related technological applications"
文摘Nitrifying biomass on ring-shaped carriers was modified to nitritating one in a relatively short period of time （37 days） by limiting the air supply, changing the aeration regime, shortening the hydraulic retention time and increasing free ammonia （FA） concentration in the moving-bed biofilm reactor （MBBR）. The most efficient strategy for the development and maintenance of nitritating biofilm was found to be the inhibition of nitrifying activity by higher FA concentrations （up to 6.5 mg/L） in the process. Reject water from sludge treatment from the Tallinn Wastewater Treatment Plant was used as substrate in the MBBR. The performance of high-surfaced biocarriers taken from the nitritating activity MBBR was further studied in batch tests to investigate nitritation and nitrification kinetics with various FA concentrations and temperatures. The maximum nitrite accumulation ratio （96.6%） expressed as the percentage of NO 2 ？ -N/NOx ？ -N was achieved for FA concentration of 70 mg/L at 36°C. Under the same conditions the specific nitrite oxidation rate achieved was 30 times lower than the specific nitrite formation rate. It was demonstrated that in the biofilm system, inhibition by FA combined with the optimization of the main control parameters is a good strategy to achieve nitritating activity and suppress nitrification.
基金supported by the National Natural Science Foundation of China (Nos. 51168028, 51168027)the Science and Technique Foundation Project for Youth of Gansu Province (No. 1107RJYA279)
文摘Free ammonia（FA） inhibition on nitrite-oxidized bacteria（NOB） and real-time control are used to achieve nitrogen removal from landfill leachate via nitrite pathway at low temperatures in sequencing batch reactor. The inhibition of FA on NOB activity during the aerobic period was prolonged using real-time control. The degree of nitrite accumulation was monitored along with variations of the ammonia-oxidizing bacteria and NOB population using fluorescence in situ hybridization techniques. It is demonstrated that the end-point of ammonia oxidization is detected from the on-line measured dissolved oxygen,oxidization–reduction potential, and p H signals, which could avoid the loss the FA inhibition on NOB caused by excess aeration. At low temperature（13.0–17.6°C）, the level of nitrite pathway rapidly increased from 19.8% to 90%, suggesting that nitritation was successfully started up at low temperature by applying syntrophic association of the FA inhibition and real-time control, and then this high level of nitrite pathway was stably maintained for as long as 233 days. Mechanism analysis shows that the establishment of nitritation was primarily the result of predominant ammonia-oxidizing bacteria developed in the nitrifying bacteria population compared to NOB. This was mainly due to a gradual reduction of nitrite amount that is available to provide energy for the growth of NOB,eventually leading to the elimination of NOB from the bacterial clusters in sequencing batch reactor sludge system.
基金supported by the Joint Innovative R&D Program of University and Industry(No.BY2014023-03)National Key Technology R&D Program of the Ministry of Scienceand Technology(No.2014BAD24B03-02)
文摘Anaerobic acidogenic fermentation with high-solid sludge is a promising method for volatile fatty acid（VFA） production to realize resource recovery. In this study, to model inhibition by free ammonia in high-solid sludge fermentation, the anaerobic digestion model No. 1（ADM1） was modified to simulate the VFA generation in batch, semicontinuous and full scale sludge. The ADM1 was operated on the platform AQUASIM 2.0.Three kinds of inhibition forms, e.g., simple inhibition, Monod and non-inhibition forms,were integrated into the ADM1 and tested with the real experimental data for batch and semi-continuous fermentation, respectively. The improved particle swarm optimization technique was used for kinetic parameter estimation using the software MATLAB 7.0. In the modified ADM1, the Ksof acetate is 0.025, the km,acis 12.51, and the KI_NH3is 0.02,respectively. The results showed that the simple inhibition model could simulate the VFA generation accurately while the Monod model was the better inhibition kinetics form in semi-continuous fermentation at pH 10.0. Finally, the modified ADM1 could successfully describe the VFA generation and ammonia accumulation in a 30 m^3full-scale sludge fermentation reactor, indicating that the developed model can be applicable in high-solid sludge anaerobic fermentation.
