Fresh osteochondral grafts find broad application in the treatment of extensive and focal damages of joint surfaces. The maintenance of chondrocyte viability of the collected grafts is of key importance. Aim: The eval...Fresh osteochondral grafts find broad application in the treatment of extensive and focal damages of joint surfaces. The maintenance of chondrocyte viability of the collected grafts is of key importance. Aim: The evaluation of chondrocyte survivability in a stable temperature of 40C in different preservative solutions is the aim of this work. Method: Chondrocyte survivability has been evaluated in saline solution (group I), Ringer solution (group II), saline with an addition of hyaluronic acid (group III) and saline enriched with glucosamine sulphate (group IV). The amount of live chondrocytes was examined on the day of collection and subsequently after 1, 2, 3,6,12, and 21 days using the Promega MTT test. Results: The highest number of live chondrocytes as calculated for 1g of hyaline cartilage after 21 days was ascertained in group IV (saline with glucosamine sulphate). The lowest number of live chondral cells was observed in group II (saline with hyaluronic acid). Chondrocyte survivability in saline (group I) was higher than in the Ringer solution (group II). Conclusions: The enrichment of saline solution with glucosamine sulphate protracts the viability of chondrocytes in fresh osteochondral gra- fts prepared for chondral transplantation.展开更多
The supply chain finance(SCF)solutions are becoming increasingly diversified with the continuous perfection of the economic system.However,financing for small and medium sized enterprises(SMEs)is still a difficult iss...The supply chain finance(SCF)solutions are becoming increasingly diversified with the continuous perfection of the economic system.However,financing for small and medium sized enterprises(SMEs)is still a difficult issue waiting to be solved by enterprises and the government in China.SCF solutions based on e-commerce platforms have developed rapidly in China that provide an alternative for SMEs when few studies have been conducted on e-commerce SCF solutions which focus on fresh agricultural products.Therefore,this research focuses on the SCF solutions applicable to e-commerce enterprises of fresh agricultural products.展开更多
文摘Fresh osteochondral grafts find broad application in the treatment of extensive and focal damages of joint surfaces. The maintenance of chondrocyte viability of the collected grafts is of key importance. Aim: The evaluation of chondrocyte survivability in a stable temperature of 40C in different preservative solutions is the aim of this work. Method: Chondrocyte survivability has been evaluated in saline solution (group I), Ringer solution (group II), saline with an addition of hyaluronic acid (group III) and saline enriched with glucosamine sulphate (group IV). The amount of live chondrocytes was examined on the day of collection and subsequently after 1, 2, 3,6,12, and 21 days using the Promega MTT test. Results: The highest number of live chondrocytes as calculated for 1g of hyaline cartilage after 21 days was ascertained in group IV (saline with glucosamine sulphate). The lowest number of live chondral cells was observed in group II (saline with hyaluronic acid). Chondrocyte survivability in saline (group I) was higher than in the Ringer solution (group II). Conclusions: The enrichment of saline solution with glucosamine sulphate protracts the viability of chondrocytes in fresh osteochondral gra- fts prepared for chondral transplantation.
文摘The supply chain finance(SCF)solutions are becoming increasingly diversified with the continuous perfection of the economic system.However,financing for small and medium sized enterprises(SMEs)is still a difficult issue waiting to be solved by enterprises and the government in China.SCF solutions based on e-commerce platforms have developed rapidly in China that provide an alternative for SMEs when few studies have been conducted on e-commerce SCF solutions which focus on fresh agricultural products.Therefore,this research focuses on the SCF solutions applicable to e-commerce enterprises of fresh agricultural products.
