Species-dependent responses of soil microbial properties to fresh leaf inputs in a subtropical forest soil in South China

Aims Forest disturbance from extreme weather events due to climate change could increase the contribution of fresh green leaves to the litter layer of soil and subsequently alter the composition and activity of the soil microbial properties and soil carbon cycling.The objective of this study was to compare the effect of naturally fallen litter and fresh leaves on the soil microbial community composition and their activities.Methods Fresh leaves and normal fallen litter were collected from four tree species(Pinus elliottii,Schima superba,Acacia mangium,A.auriculaeformis)in subtropical China and mixed with soil.Soil microbial community composition was determined using PLFAs,and its activity was quantified by soil respiration.During a 12-month period,the decomposition rate of litter was measured bimonthly using a litterbag method.Soil microbial samples were collected after 6 and 12 months.Soil respiration was measured monthly.Important Findings We found that fresh leaves decomposed faster than their conspecific fallen litter.Although total microbial biomass and bacterial biomass were similar among treatments,soil fungal biomass was higher in fresh leaf than fallen litter treatments,resulting in greater values of the Fungal phospholipid fatty acids(PLFAs)/Bacterial PLFAs ratio.Fungal PLFA values were greater for Schima superba than the other species.The effect of litter type on soil respiration was species-dependent.Specifically,fallen litter released 35%more CO_(2) than fresh leaves of the conifer P.elliottii.The opposite pattern was observed in the broadleaf species whose fresh leaf treatments emitted 17%–32%more CO_(2) than fallen litter.Given future predictions that global climate change will cause more disturbances to forests,these results indicate that conifer and broadleaf forests in subtropical China may respond differently to increased fresh litter inputs,with net soil microbial respiration decreasing in conifer forests and increasing in broadleaf forests.

Effects of Stable Chlorine Dioxide on Sterilization and Preservation of Fresh-cut Narrow-leaf Cattail

At present, chlorine dioxide has been widely used as a bactericide that may become an alternative to chlorine. In this study, fresh-cut narrow-leaf cattail was soaked with 45, 70 and 95 mg/L chlorine dioxide solution, respectively. Fresh-cut narrow-leaf cattail samples were collected regularly to determine changes in surface microbe amount, cellulose content, Vc content, reduction sugar content and sensory quality of narrow-leaf cattail, thus analyzing the effects of chlorine diox- ide on sterilization and preservation of fresh-cut narrow-leaf cattail. The results showed that all three concentrations of chlorine dioxide solution could significantly reduce the amount of microbes on the surface of fresh-cut narrow-leaf cattail and improve the edible safety of products. The initial sterilization efficiency was im- proved gradually as the concentration of chlorine dioxide increased. In addition, chlorine dioxide treatment postponed the increase of cellulose content of fresh-cut narrow-leaf cattail. However, chlorine dioxide oxidized Vc and reducing sugar, and its bleaching effect also exerted a certain impact on the sensory quality of fresh- cut narrow-leaf cattail. Based on comprehensive comparisons, 45 - 70 mg/L chlorine dioxide solution exerted the best effects on sterilization and preservation of fresh-cut narrow-leaf cattail. This study laid the foundation for the production and application of chlorine dioxide solution and promotion of rapid development of nar- row-leaf cattail industry.

How to Comprehensively Utilize the Discarded Fresh Tobacco Leaf ?

This paper aims to explore comprehensive utilization way of discarded fresh tobacco leaf. Based on modern tobacco demands on green,environmental protection and sustainable development,as well as actual situation of biogas production facility in some villages of local tobacco area,this paper selects Longwan Village of Fengshiyan Town as the experimental site and explores the rational way of discarded fresh tobacco leaf biogas fermentation. Results show that cutting and fermentation of discarded fresh tobacco leaf before adding into biogas digester is conductive to improving p H in fermentation process,reducing crust amount,and effectively improving biogas production amount and rate. The study can provide reference for effective processing and rational utilization of discarded fresh tobacco leaf.

Low Co-Cultivation Temperature at 20°C Resulted in the Reproducible Maximum Increase in Both the Fresh Weight Yield and Stable Expression of GUS Activity after <i>Agrobacterium tumefaciens</i>-Mediated Transformation of Tobacco Leaf Disks

The importance of controlled temperature during the four-days co-cultivation period was evaluated under the most physiologically relevant conditions for Agrobacterium tumefaciens-mediated transformation of tobacco (Nicotiana tabacum L. cv. Xanthi (nn, Smith)) leaf disks. We compared the effect of temperatures ranging from 15°C, 18°C, 20°C, 22°C to 25°C on the stable expression of β-glucuronidase (GUS) activity of 14 days old hygromycin-selected leaf disks, and on the increase in the fresh weight yield of 28 days old kanamycin-selected calli. The highest average of GUS activity was obtained at 20°C among the five temperatures tested although the difference between the 18°C and 20°C treatment was not statistically significant. The GUS activity at 15°C was statistically lower than those at 18°C and 20°C. The GUS activity in 22°C treatment was an intermediate between the highest (18/20°C) and second highest averages (15°C), and was not statistically significantly different. The lowest average of GUS activity was observed at 25°C. The highest increase in the plate average of fresh weight yield was obtained at 20°C among the five temperature tested. The 20°C treatment was statistically significantly better than the 15°C and 18°C treatments. The 20°C co-cultivation treatment resulted in the higher FW yield than 22°C and 25°C even though the differences were not statistically significant. In conclusion, low co-cultivation temperature at 20°C resulted in the reproducible maximum increase in both the fresh weight yield and stable expression of GUS activity after transformation of tobacco leaf disks.

剑麻专用肥对石漠化山区剑麻生长及产量的影响

石漠化山区自然条件恶劣,剑麻具备耐瘠薄、耐旱、综合利用价值高、发展前景广阔等特点,成为石漠化山区产业扶贫的较好选择。试验结果表明,剑麻新增叶片数、叶长和鲜叶产量随专用肥施入量增加而增加,每株剑麻施用专用肥量为200 g时,鲜叶产量、经济效益最高。该结论为石漠化山区剑麻高产栽培技术提供技术参考。

Impact of Progressive Pruning on Leaf Miner (Coelaenomenodera lameensis) Incidence and the Yield of Oil Palm (Elaeis guineensis) —A Case Study of Benso Oil Palm Plantation Plc, Adum Banso Estate, Ghana

The oil palm leaf miner, Coelaenomenodera lameensis, is currently the most destructive pest of oil palm in Ghana and other African oil palm growing countries, causing significant losses in fresh fruit bunch yield. Progressive pruning is an oil palm pruning method in which pruning is done at the same time as fresh fruit bunch harvesting. This study evaluated the impact of progressive pruning on leaf miner population in oil palm and how these two factors (leaf miner and progressive pruning) affect the yield of oil palm at the Benso Oil Palm Plantation Public listed company (BOPP. Plc). Five distinct blocks in the plantation were selected for observations on fronds at various ranks (33, 25, or 17) based on the degree of defoliation by counting the number of pests on leaflets at different phases of insect development. Fronds from selected plots were sampled in a Completely Randomized Design (CRD). The size of plots used for the study ranged between 19 to 45 hectares. A minimum of 78 fronds were evenly cut from each block for pest count depending on the block size. Secondary data on annual yields of fresh fruit bunches before and after the introduction of progressive pruning were also obtained from BOPP. Plc records from 2011-2020. The results from the analyzed data on leaf miner index before and after the introduction of progressive pruning showed that progressive pruning has, to a high extent (64% to 36%), reduced leaf miner populations in the plantation. Paired t-test on fresh fruit bunch yield has also revealed a significant (p < 0.001) increase in annual fresh fruit bunch yield due to progressive pruning. A regression analysis, however, revealed a lower rate of yield loss (3.05 to 2.70 tonnes) to leaf miner infestation after the introduction of progressive pruning. The study recommends progressive pruning as a key cultural practice for improving crop yields in leaf miner prone plantations.

饲粮中添加鲜木豆叶粉对育肥猪生长发育的影响

试验旨在探究鲜木豆叶粉对育肥猪生长性能、养殖经济效益、屠宰性能、肉品质及肠道微生物的影响。选取24头同批次健康、体重相近的海南黑猪,随机分为4组,每组6个重复,每个重复1头猪。对照组(A组)育肥猪饲喂基础饲粮,试验B组、C组和D组分别在基础饲粮中添加10%、15%和20%鲜木豆叶粉。预试期7 d,正式试验期270 d。结果显示,与对照组相比,各试验组育肥猪的平均日采食量均显著下降(P<0.05),试验D组育肥猪的平均日增重和末重显著下降(P<0.05)。试验C组养殖利润分别比对照组、试验B组和试验D组增加6.36%、5.68%和9.99%。与对照组相比,各试验组育肥猪的背膘厚度均显著降低(P<0.05),肉色评分均显著升高(P<0.05);试验B组和C组猪肉的pH24 h值显著升高(P<0.05);各试验组背最长肌的总氨基酸、总必需氨基酸和鲜味氨基酸含量显著升高(P<0.05);试验C组和D组饱和脂肪酸含量显著降低(P<0.05),单不饱和脂肪酸和不饱和脂肪酸含量显著升高(P<0.05);试验D组多不饱和脂肪酸含量显著升高(P<0.05)。与对照组相比,试验B组拟杆菌门相对丰度增大(P<0.05),试验B组和C组变形菌门相对丰度降低(P<0.05);各试验组大肠杆菌志贺氏属相对丰度降低(P<0.05)。研究表明,饲粮中添加鲜木豆叶粉可改善育肥猪屠宰性能、肉品质和风味,优化肠道微生物结构,减少脂肪沉积和有害菌相对丰度,以添加15%鲜木豆叶粉效果最优。

9种树种鲜叶凋落物和枯叶凋落物持水能力的比较研究

为全面认知凋落物在水土保持和水源涵养中的作用,对鲜叶凋落物和枯叶凋落物的持水能力进行比较研究。采用室内浸提法,对南方9种常见树种鲜叶凋落物、枯叶凋落物的持水特征和失水特征进行研究,并探讨鲜叶凋落物、枯叶凋落物持水能力的差异及影响因素。结果表明:9种树种鲜叶凋落物、枯叶凋落物的最大持水率分别在92.36%~237.36%、204.78%~504.88%之间;9种树种鲜叶凋落物的最大持水率均小于其枯叶凋落物的最大持水率。在失水的前12 h,大多数树种鲜叶凋落物的失水率大于其枯叶凋落物的失水率;在失水40 h时,大多数树种鲜叶凋落物的失水率小于其枯叶凋落物的失水率。鲜叶凋落物的最大持水能力主要与叶面积呈正相关,与叶形指数呈负相关;枯叶凋落物的最大持水能力与比叶面积呈正相关,与密度呈负相关。总体而言,鲜叶凋落物的持水能力弱于枯叶凋落物,但由于其现存量大,鲜叶凋落物的持水能力在水土保持和水源涵养中的作用不能被忽视。在构建森林水文模型时需纳入鲜叶凋落物的持水能力,以便更精确估算凋落物在森林水文中的作用。

茶树鲜叶色泽和化学成分与红茶适制性的关系

该研究以白叶1号、紫娟、黄金菊、福鼎大白茶4个茶树品种为材料,观察茶树芽叶性状,测定其鲜叶生化成分含量,分析不同色素含量与生化成分含量间的相关性,并制成红茶进行审评检验。结果显示,叶绿素a含量最高的为紫娟(1.21 mg/g),花青素含量最高的为紫娟(8.86 mg/g),分别为白叶1号、黄金菊和福鼎大白茶的29.53、6.47和4.32倍。相关性分析表明,色素含量与氨基酸含量呈显著负相关,与水浸出物、茶多酚和咖啡碱含量呈显著正相关;对茶树鲜叶色素含量、生化成分含量进行主成分分析发现,代表“色素因子”、“生化因子”、“滋味因子”前3个主成分累计贡献率为96.90%,可以很好地分析不同颜色(品种)鲜叶制成的红茶质量,鲜叶品质得分从高到低依次为紫鹃(T2)>黄金菊(T3)>福鼎大白茶(T4)>白叶1号(T1),与制成的红茶感官审评结果一致。紫鹃茶树品种具有高绿叶素a、叶绿叶素b、类胡萝卜素、花青素含量及高酚氨比,适合加工红茶,适制红茶品种能通过鲜叶的色素含量、生化成分含量的高低来判断。综合分析,该研究能为不同色泽茶树新梢的适制性提供数据支撑。

不同成熟度烤烟鲜叶素质及其判别函数研究

本文以重庆市酉阳土家族苗族自治县板溪基地单元烤烟品种K326为试验原料,研究不同部位、不同成熟度烟叶的单叶重、SPAD值和叶片厚度的差异性。结果表明,中部叶3项指标差异性明显,上部叶中单叶重差异不具有统计学意义,中部叶和上部叶中SPAD值差异显著。以单叶重、SPAD值和叶片厚度为自变量,成熟度为因变量,建立Fisher线性判别函数,并对其判别效果进行检验:中部叶中,对CM3烟叶的判别准确率最高,为70.7%;其次为CM1烟叶,为65.6%;最低是CM2烟叶,准确率为59.2%;整体准确率为65.1%。上部叶中,对BM3烟叶判别的准确率为98.8%,BM2烟叶准确率为40.0%,BM1烟叶准确率为56.1%,整体准确率为65.0%。

新疆地区4个红豆草新品系的生产性能评价

【目的】为客观评价红豆草新品系的生产性能,为新品系的示范推广及品种审定提供基础数据。【方法】试验以新疆农业大学选育的3个红豆草新品系ND056-1、ND056-2、ND056-3为材料,以奇台红豆草为对照(CK),通过观测生育期、株高、干鲜比、茎叶比、鲜草产量、干草产量等指标,对其生产性能进行综合评价。【结果】研究结果表明,四个材料的物候期无差异,ND056-3的平均株高最高为104.2 cm,ND056-2的次之为104.13 cm,CK最低为103.07 cm;ND056-1的干鲜比最高为30.15%,是对照的1.22倍,ND056-2最低为23.73%,是对照的0.96倍;ND056-3的叶茎比最高(1.2266),ND056-2次之(0.9918),分别是对照的1.1倍和1.38倍;全年鲜草产量中,ND056-3的最高为70.58 t/hm^(2),ND056-1次之为66.25 t/hm^(2),分别是对照的1.247倍和1.170倍;全年干草产量中,ND056-1的最高为19.89 t/hm^(2),ND056-3次之为19.32 t/hm^(2),分别是对照的1.30倍和1.27倍。【结论】综合分析认为,新品系ND056-3适合作为青鲜草利用,ND056-1适合作为干草利用。

基于荷叶醇提物-壳聚糖的复合涂膜对低温蓝莓的保鲜效果

为探究荷叶醇提物(LLAE)与壳聚糖(CH)复合涂膜对低温贮藏蓝莓贮藏品质的影响。分别将不同质量浓度的LLAE(0、1.0、2.0及4.0 g/L)与CH复配对蓝莓进行涂膜处理,并在4℃条件对各处理组蓝莓的贮藏品质指标和防御酶系活性进行测定分析。结果表明:与对照组相比,LLAE-CH复合涂膜处理组可有效减缓蓝莓可滴定酸含量和硬度的下降,减小可溶性固形物含量、维生素C含量、花青素含量等营养成分的损失,并有效抑制采后蓝莓果实的失重率、腐烂率、呼吸强度、丙二醛含量的上升,且提高过氧化氢酶活性、过氧化物酶活性和总酚含量。因此,LLAE-CH复合涂膜有效提升蓝莓果实贮藏品质,减少营养物质消耗,提高其抗氧化活性,从而有效延长了蓝莓的货架期,且4.0 g/L LLAE-CH复合涂膜对蓝莓果实的保鲜效果最佳。

A Study on Physiological Character of Fresh Tea Leaves in Different Cold-Resistant Varieties

In order to reveal the cold resistant mechanism of tea plant,two varieties with different cold resistances were studied.The activities of catalase(CAT),peroxidase(POD),superoxide dismutase(SOD) and polyphenol oxidase(PPO) were investigated,and the contents of soluble protein,sugar and malondialdehyde(MDA) were determined in fresh tea leaves in the autumn.The results showed that the activities of POD and the content of soluble sugar were both much higher in the variety with strong cold resistance,and the content of soluble protein was associated with the cold resistance,while the cold resistance of the plant was not related with the activities of the CAT,SOD and PPO,and the content of MDA.It is considered that the activity of POD and contents of soluble sugar could be used as indicators to identify the cold resistance of tea varieties.

不适用烟叶去除方式对烟叶光合速率及产质量的影响

为探讨适宜皖南烤烟适宜的去叶方式,采用田间小区试验,以云烟87为材料,根据不适用烟叶去除时间和数量设置4个处理:B1处理(打顶前10 d摘除3片脚叶、打顶时摘除1片顶叶),B2处理(打顶前5 d摘除3片脚叶、打顶时摘除1片顶叶),B3处理(打顶后5 d摘除4片脚叶),B4处理(打顶后10 d摘除4片脚叶),从烟叶光合特性、产量产值、化学成分、外观和感官质量等全面评价优烟叶优化结构的作用。结果表明,摘除不适用烟叶显著影响烟株剩余烟叶的净光合速率,B3处理较B1处理中部叶和上部叶的净光合速率分别明显降低46%和38%。B3处理烟叶上等烟比例和中上等烟比例均较高,分别较B1处理提高4.7和1.5百分点。B3处理可提高烟叶感官质量,可能是该去叶方式能够合理调控烤烟的光合作用和代谢强度,促进烤后烟叶化学成分更加协调,B3处理能够保障种烟效益、提高烟叶等级结构、提升烟叶感官质量。综合可见,打顶后5 d摘除4片脚叶是皖南烟区适宜的优化烟叶结构措施。

青砖茶原料采收方式与嫩度对茶叶氟含量及品质的影响

目的 探究青砖茶原料采收方式与嫩度对茶叶氟含量及品质的影响,探索有效降低青砖茶氟含量的青砖茶原料采收新技术。方法 选择3个不同地点的茶园对比分析青砖茶采收方式对氟含量的影响,通过感官评价和理化指标检测分析不同嫩度原料加工青砖茶的品质差异。结果 在传统采收方式下, 3个试验茶厂7月和11月每轮次茶叶氟含量平均都在600 mg/kg以上。新的采收方式将传统的采收两轮改为四轮。随着采收轮次的增加(即采收时期的推移),茶叶氟含量呈现下降趋势,新采收方式的茶叶氟含量显著低于传统方式22%~51%。不同枝梢部位含氟量有极显著差异。随着嫩度的下降,氟含量呈先升后降的趋势,以红茎后段的含量最高,绿茎的含量最低。青砖茶感官品质分析表明,随着嫩度的下降,外形调匀度、汤色黄色度下降;带灰白色茎梗的原料加工的成品茶粗老气味较重,滋味较淡薄,提示青砖茶原料并非越老越好。理化分析结果表明,随着嫩度的下降,水浸出物、茶多酚及其氧化产物、氨基酸、可溶性糖含量呈降低趋势,说明利用太老的原料加工青砖茶时不仅品质低下,而且氟含量超标。结论 青砖茶原料宜控制在一定成熟度范围,以成熟绿茎枝叶为宜,生产上可以采取增加采割轮次、缩短生长期、提高采割嫩度等技术措施来有效降低茶叶中的氟含量。

荷叶与海藻酸钠复合鲜湿面配方研究

为充分利用荷叶资源,采用添加荷叶粉、海藻酸钠和减肥茶汤(山楂、陈皮、薏米、荷叶熬制)开发具备荷叶风味的鲜湿面。通过单因素和正交试验相结合的方法,以膨胀率、断条率、感官评价等为评价指标研究荷叶鲜湿面配方。结果表明:影响荷叶鲜湿面制作工艺因素大小依次为荷叶粉>海藻酸钠>减肥茶汤。最佳配方用量荷叶粉用量10 g/kg,海藻酸钠用量2 g/kg,减肥茶汤用量300 mL/kg。海藻酸钠可以弥补荷叶所造成的面条蒸煮品质恶化,所研制鲜湿面色泽翠绿、口感细腻爽滑,具独特荷叶清香。

机采名优茶识别研究与试验——基于近红外光谱技术

机械化采茶过程中,采摘到的碎叶、名优茶鲜叶以及能够加工成大宗茶的带叶枝条和完整单叶混杂在一起,影响了名优茶鲜叶的进一步加工。为此,将机采茶鲜叶作为试验样本进行研究,基于近红外光谱技术和机器学习方法,对名优茶和大宗茶鲜叶样本中的单个完整叶片样本进行分类识别并得出结果。在事先去除带叶枝条、碎叶和单芽之后,通过搭建试验台的方式采集到两类样本的光谱数据,采用标准正态变量变换结合多元散射校正预处理后,使用保留20个主成分的PCA方法进行降维,使用线性判别和支持向量机算法建立分类模型,得到两类样本的识别情况。试验结果表明:两种算法均可识别出名优茶样本,且使用线性判别算法构建的分类模型识别效果最佳,在样本数200、300、400下的识别准确率分别达到98.5%、96.67%和96%。此研究提取的分类模型,可以为实际加工中的名优茶机械化采摘和识别提供技术参考。

不同冷冻胁迫时间对茶鲜叶中酶活性和营养成分的影响

为探究“倒春寒”和模拟冻害条件下茶树鲜叶中酶活性与营养成分的变化,以“倒春寒”发生时茶树实际受冻害和模拟冻害的新梢为研究材料,比较了不同冷冻胁迫时间下茶树鲜叶中多酚氧化酶(PPO)、过氧化物酶(POD)和β-葡萄糖苷酶(β-GC)酶活性及营养成分变化的差异。结果表明,在-3~-5℃冷冻4~6 h的条件下,模拟冻害的鲜叶外形最接近实际受冻情况。随着冷冻时间的增加,鲜叶中PPO酶活性呈现先升高后降低趋势,POD酶活性整体趋于稳定,而β-GC酶的活性整体呈现降低趋势。此外,在冷冻过程中鲜叶茶黄素含量显著增加,酯型儿茶素含量显著降低。这些研究可为茶树遭受冻害时鲜叶质量评估与再利用,以及茶树响应“倒春寒”机制研究提供一定理论依据。

南方红豆杉鲜叶浸提液对3种药用植物种子萌发与幼苗生长的化感作用

为给红豆杉间作套种适栽药用植物提供理论依据,本试验以蒸馏水为对照(CK),对3种药用植物(黄芩、紫花苜蓿和牛蒡)进行2、6 g·L^(-1)和10 g·L^(-1)南方红豆杉鲜叶浸提液处理,研究其对3种药用植物种子萌发、幼苗生长、光合色素含量、丙二醛含量及保护酶活性的影响,分析南方红豆杉鲜叶浸提液对3种药用植物的化感作用。结果表明:南方红豆杉鲜叶浸提液对黄芩和紫花苜蓿种子萌发呈抑制作用,对牛蒡种子萌发呈高浓度促进作用;对紫花苜蓿的根长呈抑制作用,对黄芩的根长呈促进作用。不同浓度南方红豆杉浸提液对黄芩、紫花苜蓿、牛蒡的丙二醛含量、光合色素含量和抗氧化物酶活性的影响不同,其中2 g·L^(-1)浸提液处理紫花苜蓿的丙二醛含量最高,与CK相比提高141.05%(P<0.05);6 g·L^(-1)浸提液处理显著提高牛蒡光合色素含量;2 g·L^(-1)和6 g·L^(-1)浸提液下牛蒡的超氧化物歧化酶(SOD)活性和过氧化物酶(POD)活性最高,比CK分别提高11.21%和164.00%;10 g·L^(-1)浸提液下黄芩的过氧化氢酶(CAT)活性最高,比CK提高345.46%。在红豆杉浸提液对3种药用植物的化感综合效应中,对紫花苜蓿的化感综合效应呈现低浓度促进高浓度抑制态势,其中2 g·L^(-1)浓度下化感综合效应值为0.007;6 g·L^(-1)浓度下牛蒡和10 g·L^(-1)浓度下黄芩的化感综合效应值最大,分别为0.242和0.098。综上,红豆杉浸提液影响3种药用植物的种子萌发和幼苗生长,其中6 g·L^(-1)浓度显著抑制紫花苜蓿的种子萌发和幼苗生长;6 g·L^(-1)浓度对牛蒡的化感综合效应作用最显著。

不同饲料育条件下柞蚕幼虫中肠细菌的多样性分析

为了探讨不同饲料饲育条件下柞蚕(Antheraea pernyi)幼虫肠道细菌群落的变化情况,采用新鲜柞叶育(DJY)和人工饲料育(DJS)2种模式饲育柞蚕1~2龄幼虫,收集柞蚕3龄幼虫中肠样品,通过16S rRNA高通量测序技术分析其肠道菌群的组成和多样性差异。结果显示,在门水平上,人工饲料育与鲜柞叶育柞蚕幼虫肠道优势菌组成相似,均为变形杆菌门(Proteobacteria)、厚壁菌门(Firmicutes)和放线菌门(Actinobacteria),但占比不同,DJS组柞蚕中肠内三者占比分别为83.55%、5.90%和4.77%,DJY组柞蚕中肠内则为90.96%、1.48%和2.04%;在属水平上,二者共同的优势菌以不动杆菌属(Acinetobacter)为主,DJS组柞蚕中肠内其他优势菌属按占比由高到低依次为葡萄球菌属(Staphylococcus)、假单胞菌属(Pseudomonas)、红细菌属(Mangrovibacter)、贝氏谷氨酸杆菌属(Glutamicibacter)和肠杆菌属(Enterobacter),DJY组柞蚕中肠中则为泛菌属(Pantoea)、肠杆菌属(Enterobacter)、弧菌属(Vibrio)、贝氏谷氨酸杆菌属(Glutamicibacter)和葡萄球菌属(Staphylococcus)。此外,菌群数目和丰富度分析表明,DJS组柞蚕中肠细菌物种组成的丰富度和物种多样性比DJY组更高。菌群差异性分析结果显示,DJS组细菌丰富度存在显著差异的有假单胞菌属某菌(Pseudomonas-sp-20-2)和约氏不动杆菌(Acinetobacter johnsonii),DJY组细菌丰富度存在显著差异的有醋酸钙不动杆菌(Acinetobacter calcoaceticus)和古氏不动杆菌(Acinetobacter guillouiae)。