The aim of the present study was to evaluate the nutritional quality of green and purple eggplant, their antioxidant activity and their nutritional efficacy on Wistar rats. For nutritional quality, the parameters meas...The aim of the present study was to evaluate the nutritional quality of green and purple eggplant, their antioxidant activity and their nutritional efficacy on Wistar rats. For nutritional quality, the parameters measured are dry matter, protein, lipid, ash, carbohydrate, iron, phosphorus, calcium, magnesium and energy content. For antioxidant activity, the parameters measured are 50% DDPH free radical inhibition concentration and total polyphenol content. Nutritional efficacy was evaluated in rats fed the control diet and in rats fed the three treated diets containing eggplant meal obtained by replacing 5%, 10%, and 15% of the control diet. The parameters measured are the amount consumed, the weight of the animal and target organs, and hematologic parameters. The results of the nutritional analysis show the following values: 13.31% protein, 2.66% lipids, 0.84% calcium, 0.12% magnesium, 0.43% phosphorus for the Solanum aethiopicum L. species and 13.47% protein, 3.66% lipids, 0.36% calcium, 0.22% magnesium, 0.35% phosphorus for the Solanum melongena L. species. In terms of antioxidant activity, we obtained DDPH inhibition percentages of 40.28 mg/ml for Solanum aethiopicum L. and 12.42 mg/ml for Solanum melongena L., respectively. Finally, hematologic and anthropometric tests showed that for the different diets used, weight loss and an increase in hematologic parameters were observed in the rats tested. This study showed that eggplant has interesting nutritional characteristics and antioxidant activity, contributing to an increase in weight and anthropometric parameters.展开更多
Glucosinolates(GLS) contribute to the unique flavour, nutrition, and plant defence of the Cruciferous vegetables. Understanding the GLS changes through postharvest processing is essential for defined preservation. In ...Glucosinolates(GLS) contribute to the unique flavour, nutrition, and plant defence of the Cruciferous vegetables. Understanding the GLS changes through postharvest processing is essential for defined preservation. In this study, four different fresh-cut types, whole flower(W),floret(F), quarterly cut floret(QF) and shredded floret(FS) of broccoli, were stored for 0, 1, 2 and 3 day(s) to explore GLS responses to postharvest treatments. As a result, seven GLS were identified, mainly including glucoraphanin(RAA), neoglucobrassicin(NEO), and glucobrassicin(GBC)and accounting for 52.69%, 20.12% and 14.99% of the total GLS(21.92 ± 0.48) μmol · g ^(-1 )DW, respectively. FS had the sharpest decrease in GLS after three days of storage(6.55 ± 0.37) μmol · g-1DW, while QF had the least(10.16 ± 0.33) μmol · g ^(-1 )DW. All GLS components decreased over storage, except for 4-methoxyglucobrassicin(4 ME) in FS and QF, suggesting its key role in serious wound defence. The results suggested certain postharvest approaches influenced the flavour and nutrition of broccoli.展开更多
Low temperature is the most common abiotic stress factors during the eggplant cultivation in solar greenhouses.Melatonin plays important roles in plant resistance to low temperature.However,the role of melatonin in re...Low temperature is the most common abiotic stress factors during the eggplant cultivation in solar greenhouses.Melatonin plays important roles in plant resistance to low temperature.However,the role of melatonin in regulating chilling tolerance and extending the preharvest shelf life of eggplant fruits is still unknown.In this study,we investigated the effects of exogenous melatonin on eggplant plants and fruits in response to low temperature.Under simulated low-temperature conditions,exogenous melatonin significantly relieved the chilling symptoms of seedlings by reducing reactive oxygen species (ROS) and malondialdehyde (MDA) levels and relative leakage rates.These reductions were caused by higher superoxide dismutase (SOD) and catalase (CAT) activities and increased endogenous polyamine and melatonin levels compared with those in untreated seedlings.Notably,the expression levels of SOD,CAT1/2,and polyamine synthesis genes (ADC and ODC) were also increased by 100μmol·L~(-1)melatonin,as well as those of genes involved in melatonin synthesis (TDC,T5H,SNAT,ASMT,and COMT) and cold regulation (COR1,CBFa/b,and ZAT2/6/12).To further investigate the effects of melatonin on eggplant leaves and fruits under natural low temperature conditions,100μmol·L~(-1)melatonin was sprayed on the functional leaves at three days before commodity maturation.Melatonin significantly alleviated chilling injury in the leaves and pericarp and extended the preharvest shelf life of the fruit by increasing the expression of COR1,CBF,ZAT2/6/12,and API5 and decreasing the expression of senescence-related genes (NCED1/2 and SAG12).Therefore,100μmol·L~(-1)melatonin improved chilling tolerance and fruit shelf life by upregulating ZAT2/6/12 to affect ROS-and senescence-related processes,which provides a reference for alleviating cold stress and extending the preharvest fruit shelf life in eggplant.展开更多
Water stress early detection is essential for precision farming to improve crop productivity and product quality. The methods usually used are destructive, long and expensive. In this work, we used hyperspectral chlor...Water stress early detection is essential for precision farming to improve crop productivity and product quality. The methods usually used are destructive, long and expensive. In this work, we used hyperspectral chlorophyll fluorescence technology as a rapid, non-destructive approach to detect the water deficiency of eggplant plants using their spectral footprint. So, an experiment was made on 54 eggplant plants subjected to three water treatments: normal irrigation (T<sub>100</sub>), intermediate irrigation (T<sub>50</sub>) and no irrigation (T<sub>0</sub>). The fluorescence spectra were acquired in vivo and in situ using a USB4000 spectrometer from Ocean optics. For the classification of the plants subjected to three water treatments, we used three pretreatments of the raw hyperspectral data in order to suppress the non-informative variability present in these spectra and to obtain robust models. These are the Savitzky-Golay smoothing (SG), the standard normal variable (SNV) and the first derivative of Savitzky-Golay (SG-D1). The preprocessed data were then subjected to two partial least squares discriminant analyses (PLS-DA): Hard PLS-DA and Soft PLS-DA. These statistical approaches are suitable for large samples as it reduces the dimensionality of the data but improves the accuracy of the prediction. The SG-D1 combined with the Soft PLS-DA gave the best discrimination of plants with scores of sensitivity, specificity and total efficiency respectively of 97.33%, 94% and 95% for calibration, 6 days after hydric stress induction. For the plants used for the prediction, the scores are 86%, 91% and 90% respectively. This study shows that hyperspectral chlorophyll fluorescence spectroscopy is a fast and non-destructive technology allowing early detection of water stress in eggplant plants.展开更多
In this study,wild eggplant germplasm No.M239,which is highly susceptible to Verticillium wilt,was used as the experimental material.The physiological and biochemical indices(SOD,PAL,MDA and soluble protein)of M239 ro...In this study,wild eggplant germplasm No.M239,which is highly susceptible to Verticillium wilt,was used as the experimental material.The physiological and biochemical indices(SOD,PAL,MDA and soluble protein)of M239 roots were measured at different times(0,12,24,36,48,60 and 72 h)post inoculation with Verticillium dahliae,and the key time points for the M239 response to Verticillium wilt infection were screened.Then,RNA-Seq technology was used to screen the differentially expressed genes(DEGs)in M239 roots at 0,12 and 48 h post-inoculation(hpi).The transcriptional results of M239 were also compared with those resistance genes from some reported wild relative Solanum species(S.sisymbriifolium and S.aculeatissimum).Then some DEGs were chosen for validation by qRT–PCR.The results showed that 12 and 48 hpi were the turning points in the changes in all physiological and biochemical indices.A total of 6,783 DEGs were identified by RNA-Seq,including 6,141 DEGs(3,046 upregulated and 3,095 downregulated)at the M_12 h vs.M_0 h,1,903 DEGs(792 upregulated and 1,111 downregulated)at M_48 h vs.M_12 h,and 1,261 DEGs that appeared simultaneously in both stages.KEGG enrichment analysis showed that there were 5 metabolic pathways enriched from DEGs,which were mostly related to primary metabolism,such as glycolysis,amino acid and ribosome biogenesis.Compared with the NCBI non-redundant protein(NR)database,one Ve2 homologous gene and 8 PR protein-related genes were screened.Transcription factor analysis showed that there were a large number of DEGs,such as MYB,AP2-EREBP,bHLH,NAC and Orphans in the two stages.Compared with the reported Verticillium wilt-resistant wild eggplant species,it was found that there were fewer genes and enriched metabolic pathways in the M239 response to Verticillium wilt infection,and it also lacked the response of some known key resistance genes.These results proved that the above resistance genes and metabolic pathways played a key role in the wild eggplant response to V.dahliae infection.展开更多
[Objectives]The paper was to study the effect of magnetic field technology on physicochemical properties of fresh-cut apple during storage.[Methods]Using fresh-cut apples as materials,magnetic field assisted refrigera...[Objectives]The paper was to study the effect of magnetic field technology on physicochemical properties of fresh-cut apple during storage.[Methods]Using fresh-cut apples as materials,magnetic field assisted refrigeration and conventional refrigeration control groups were set up,and the preservation effects of magnetic field assisted refrigeration on fresh-cut apples were tested by measuring the changes of microorganisms and physicochemical indicators.In this experiment,the conditions of magnetic field assisted refrigeration group was set as follows:temperature 4℃,magnetic field intensity 5 mT,static magnetic field,and storage for 6 d.[Results]At 6 d post storage,the weight loss rate,total number of colonies and total number of fungi of fresh-cut apples increased;the L value and hardness showed a downward trend;the soluble solid content first increased and then decreased;and the total acid decreased first and then increased.The above indicators in magnetic field assisted refrigeration group changed more gently than that in conventional refrigeration group.Compared with conventional assisted refrigeration,the weight loss rate of fresh-cut apples treated by magnetic field combined with 4℃refrigeration was reduced by 68.00%;the hardness was increased by 12.23%;the total number of colonies and total number of fungi were reduced by 64.15%and 37.50%;and the L value did not change much.High content of soluble solids and total acid was maintained.[Conclusions]Magnetic field assisted refrigeration delays quality deterioration of fresh-cut apples during storage,prolongs shelf life,and further improves their edible quality and commodity value.展开更多
文摘The aim of the present study was to evaluate the nutritional quality of green and purple eggplant, their antioxidant activity and their nutritional efficacy on Wistar rats. For nutritional quality, the parameters measured are dry matter, protein, lipid, ash, carbohydrate, iron, phosphorus, calcium, magnesium and energy content. For antioxidant activity, the parameters measured are 50% DDPH free radical inhibition concentration and total polyphenol content. Nutritional efficacy was evaluated in rats fed the control diet and in rats fed the three treated diets containing eggplant meal obtained by replacing 5%, 10%, and 15% of the control diet. The parameters measured are the amount consumed, the weight of the animal and target organs, and hematologic parameters. The results of the nutritional analysis show the following values: 13.31% protein, 2.66% lipids, 0.84% calcium, 0.12% magnesium, 0.43% phosphorus for the Solanum aethiopicum L. species and 13.47% protein, 3.66% lipids, 0.36% calcium, 0.22% magnesium, 0.35% phosphorus for the Solanum melongena L. species. In terms of antioxidant activity, we obtained DDPH inhibition percentages of 40.28 mg/ml for Solanum aethiopicum L. and 12.42 mg/ml for Solanum melongena L., respectively. Finally, hematologic and anthropometric tests showed that for the different diets used, weight loss and an increase in hematologic parameters were observed in the rats tested. This study showed that eggplant has interesting nutritional characteristics and antioxidant activity, contributing to an increase in weight and anthropometric parameters.
基金supported by the Collaborative Innovation Center of the Beijing Academy of Agriculture and Forestry Sciences(Grant No.KJCX201915)the Youth Scientific Research Funds of Beijing Academy of Agriculture and Forestry Sciences(Grant No.QNJJ201914)the Innovation and Capacity-building Project of Beijing Academy of Agriculture and Forestry Sciences(Grant No.KJCX20200213).
文摘Glucosinolates(GLS) contribute to the unique flavour, nutrition, and plant defence of the Cruciferous vegetables. Understanding the GLS changes through postharvest processing is essential for defined preservation. In this study, four different fresh-cut types, whole flower(W),floret(F), quarterly cut floret(QF) and shredded floret(FS) of broccoli, were stored for 0, 1, 2 and 3 day(s) to explore GLS responses to postharvest treatments. As a result, seven GLS were identified, mainly including glucoraphanin(RAA), neoglucobrassicin(NEO), and glucobrassicin(GBC)and accounting for 52.69%, 20.12% and 14.99% of the total GLS(21.92 ± 0.48) μmol · g ^(-1 )DW, respectively. FS had the sharpest decrease in GLS after three days of storage(6.55 ± 0.37) μmol · g-1DW, while QF had the least(10.16 ± 0.33) μmol · g ^(-1 )DW. All GLS components decreased over storage, except for 4-methoxyglucobrassicin(4 ME) in FS and QF, suggesting its key role in serious wound defence. The results suggested certain postharvest approaches influenced the flavour and nutrition of broccoli.
基金Introduction of Talents for Scientific Research of State Key Laboratory of North China Crop Improvement and Regulation (Grant No.NCCIR2020RC-11)Hebei Fruit Vegetables Seed Industry Science and Technology Innovation Team Project (Grant No.21326309D)+2 种基金Vegetable Innovation Team Project of Hebei Modern Agricultural Industrial Technology System (Grant No.HBCT2018030203)Introduction of Talents for Scientific Research of Hebei Agriculture University (Grant No.YJ2020048)Basic Scientific Research Funds of Provincial Universities of Hebei Province (Grant No.KY2021056) for the provision of funds。
文摘Low temperature is the most common abiotic stress factors during the eggplant cultivation in solar greenhouses.Melatonin plays important roles in plant resistance to low temperature.However,the role of melatonin in regulating chilling tolerance and extending the preharvest shelf life of eggplant fruits is still unknown.In this study,we investigated the effects of exogenous melatonin on eggplant plants and fruits in response to low temperature.Under simulated low-temperature conditions,exogenous melatonin significantly relieved the chilling symptoms of seedlings by reducing reactive oxygen species (ROS) and malondialdehyde (MDA) levels and relative leakage rates.These reductions were caused by higher superoxide dismutase (SOD) and catalase (CAT) activities and increased endogenous polyamine and melatonin levels compared with those in untreated seedlings.Notably,the expression levels of SOD,CAT1/2,and polyamine synthesis genes (ADC and ODC) were also increased by 100μmol·L~(-1)melatonin,as well as those of genes involved in melatonin synthesis (TDC,T5H,SNAT,ASMT,and COMT) and cold regulation (COR1,CBFa/b,and ZAT2/6/12).To further investigate the effects of melatonin on eggplant leaves and fruits under natural low temperature conditions,100μmol·L~(-1)melatonin was sprayed on the functional leaves at three days before commodity maturation.Melatonin significantly alleviated chilling injury in the leaves and pericarp and extended the preharvest shelf life of the fruit by increasing the expression of COR1,CBF,ZAT2/6/12,and API5 and decreasing the expression of senescence-related genes (NCED1/2 and SAG12).Therefore,100μmol·L~(-1)melatonin improved chilling tolerance and fruit shelf life by upregulating ZAT2/6/12 to affect ROS-and senescence-related processes,which provides a reference for alleviating cold stress and extending the preharvest fruit shelf life in eggplant.
文摘Water stress early detection is essential for precision farming to improve crop productivity and product quality. The methods usually used are destructive, long and expensive. In this work, we used hyperspectral chlorophyll fluorescence technology as a rapid, non-destructive approach to detect the water deficiency of eggplant plants using their spectral footprint. So, an experiment was made on 54 eggplant plants subjected to three water treatments: normal irrigation (T<sub>100</sub>), intermediate irrigation (T<sub>50</sub>) and no irrigation (T<sub>0</sub>). The fluorescence spectra were acquired in vivo and in situ using a USB4000 spectrometer from Ocean optics. For the classification of the plants subjected to three water treatments, we used three pretreatments of the raw hyperspectral data in order to suppress the non-informative variability present in these spectra and to obtain robust models. These are the Savitzky-Golay smoothing (SG), the standard normal variable (SNV) and the first derivative of Savitzky-Golay (SG-D1). The preprocessed data were then subjected to two partial least squares discriminant analyses (PLS-DA): Hard PLS-DA and Soft PLS-DA. These statistical approaches are suitable for large samples as it reduces the dimensionality of the data but improves the accuracy of the prediction. The SG-D1 combined with the Soft PLS-DA gave the best discrimination of plants with scores of sensitivity, specificity and total efficiency respectively of 97.33%, 94% and 95% for calibration, 6 days after hydric stress induction. For the plants used for the prediction, the scores are 86%, 91% and 90% respectively. This study shows that hyperspectral chlorophyll fluorescence spectroscopy is a fast and non-destructive technology allowing early detection of water stress in eggplant plants.
基金supported by the National Natural Science Foundation of China(31960594)the Yunnan Fundamental Research Projects(202201AT070074,2019FB059)the Yunnan Technical Innovation Talent Training Project(202205AD160029).
文摘In this study,wild eggplant germplasm No.M239,which is highly susceptible to Verticillium wilt,was used as the experimental material.The physiological and biochemical indices(SOD,PAL,MDA and soluble protein)of M239 roots were measured at different times(0,12,24,36,48,60 and 72 h)post inoculation with Verticillium dahliae,and the key time points for the M239 response to Verticillium wilt infection were screened.Then,RNA-Seq technology was used to screen the differentially expressed genes(DEGs)in M239 roots at 0,12 and 48 h post-inoculation(hpi).The transcriptional results of M239 were also compared with those resistance genes from some reported wild relative Solanum species(S.sisymbriifolium and S.aculeatissimum).Then some DEGs were chosen for validation by qRT–PCR.The results showed that 12 and 48 hpi were the turning points in the changes in all physiological and biochemical indices.A total of 6,783 DEGs were identified by RNA-Seq,including 6,141 DEGs(3,046 upregulated and 3,095 downregulated)at the M_12 h vs.M_0 h,1,903 DEGs(792 upregulated and 1,111 downregulated)at M_48 h vs.M_12 h,and 1,261 DEGs that appeared simultaneously in both stages.KEGG enrichment analysis showed that there were 5 metabolic pathways enriched from DEGs,which were mostly related to primary metabolism,such as glycolysis,amino acid and ribosome biogenesis.Compared with the NCBI non-redundant protein(NR)database,one Ve2 homologous gene and 8 PR protein-related genes were screened.Transcription factor analysis showed that there were a large number of DEGs,such as MYB,AP2-EREBP,bHLH,NAC and Orphans in the two stages.Compared with the reported Verticillium wilt-resistant wild eggplant species,it was found that there were fewer genes and enriched metabolic pathways in the M239 response to Verticillium wilt infection,and it also lacked the response of some known key resistance genes.These results proved that the above resistance genes and metabolic pathways played a key role in the wild eggplant response to V.dahliae infection.
基金Supported by Rural Revitalization Science and Technology Innovation Boost Action Plan of Shandong Province“Apple Step Processing and High Value Utilization Technology Research and Product Creation”(2022TZXD007-04).
文摘[Objectives]The paper was to study the effect of magnetic field technology on physicochemical properties of fresh-cut apple during storage.[Methods]Using fresh-cut apples as materials,magnetic field assisted refrigeration and conventional refrigeration control groups were set up,and the preservation effects of magnetic field assisted refrigeration on fresh-cut apples were tested by measuring the changes of microorganisms and physicochemical indicators.In this experiment,the conditions of magnetic field assisted refrigeration group was set as follows:temperature 4℃,magnetic field intensity 5 mT,static magnetic field,and storage for 6 d.[Results]At 6 d post storage,the weight loss rate,total number of colonies and total number of fungi of fresh-cut apples increased;the L value and hardness showed a downward trend;the soluble solid content first increased and then decreased;and the total acid decreased first and then increased.The above indicators in magnetic field assisted refrigeration group changed more gently than that in conventional refrigeration group.Compared with conventional assisted refrigeration,the weight loss rate of fresh-cut apples treated by magnetic field combined with 4℃refrigeration was reduced by 68.00%;the hardness was increased by 12.23%;the total number of colonies and total number of fungi were reduced by 64.15%and 37.50%;and the L value did not change much.High content of soluble solids and total acid was maintained.[Conclusions]Magnetic field assisted refrigeration delays quality deterioration of fresh-cut apples during storage,prolongs shelf life,and further improves their edible quality and commodity value.