To reveal the genetic diversity of maize germplasm resources in Guizhou Province, we screened 20 pairs of SSR primers out of 100 pairs tested for amplify-ing the al eles in 21 shares of maize germplasm col ected there...To reveal the genetic diversity of maize germplasm resources in Guizhou Province, we screened 20 pairs of SSR primers out of 100 pairs tested for amplify-ing the al eles in 21 shares of maize germplasm col ected there. Total y 85 al eles were identified from the experimental materials, and each primer pair amplified 2-9 al eles, with an average of 4.25. The polymorphism information content (PIC) values varied from 0.19 to 0.74, with an average of 0.50. The clustering analysis by UPG-MA indicated that the genetic distance among the tested lines was 0.22-0.63, with an average of 0.43. This provided the theoretical foundations for establishing the finger print chromatogram of maize, further for exploring and utilizing maize germplasms Guizhou Province.展开更多
基金Supported by Special Fund of Guizhou Academy of Agricultural Science[2X(2007)004]~~
文摘To reveal the genetic diversity of maize germplasm resources in Guizhou Province, we screened 20 pairs of SSR primers out of 100 pairs tested for amplify-ing the al eles in 21 shares of maize germplasm col ected there. Total y 85 al eles were identified from the experimental materials, and each primer pair amplified 2-9 al eles, with an average of 4.25. The polymorphism information content (PIC) values varied from 0.19 to 0.74, with an average of 0.50. The clustering analysis by UPG-MA indicated that the genetic distance among the tested lines was 0.22-0.63, with an average of 0.43. This provided the theoretical foundations for establishing the finger print chromatogram of maize, further for exploring and utilizing maize germplasms Guizhou Province.