Evaluation of the intracellular lipid-lowering effect of polyphenols extract from highland barley in HepG2 cells

Active ingredients from highland barley have received considerable attention as natural products for developing treatments and dietary supplements against obesity.In practical application,the research of food combinations is more significant than a specific food component.This study investigated the lipid-lowering effect of highland barley polyphenols via lipase assay in vitro and HepG2 cells induced by oleic acid(OA).Five indexes,triglyceride(TG),total cholesterol(T-CHO),low density lipoprotein-cholesterol(LDL-C),aspartate aminotransferase(AST),and alanine aminotransferase(ALT),were used to evaluate the lipidlowering effect of highland barley extract.We also preliminary studied the lipid-lowering mechanism by Realtime fluorescent quantitative polymerase chain reaction(q PCR).The results indicated that highland barley extract contains many components with lipid-lowering effects,such as hyperoside and scoparone.In vitro,the lipase assay showed an 18.4%lipase inhibition rate when the additive contents of highland barley extract were 100μg/m L.The intracellular lipid-lowering effect of highland barley extract was examined using 0.25 mmol/L OA-induced HepG2 cells.The results showed that intracellular TG,LDL-C,and T-CHO content decreased by 34.4%,51.2%,and 18.4%,respectively.ALT and AST decreased by 51.6%and 20.7%compared with the untreated hyperlipidemic HepG2 cells.q PCR results showed that highland barley polyphenols could up-regulation the expression of lipid metabolism-related genes such as PPARγand Fabp4.

Pharmacological effects of bioactive agents in earthworm extract:A comprehensive review

This review compiles information from the literature on the chemical composition,pharmacological effects,and molecular mechanisms of earthworm extract(EE)and suggests possibilities for clinical translation of EE.We also consider future trends and concerns in this domain.We summarize the bioactive components of EE,including G-90,lysenin,lumbrokinase,antimicrobial peptides,earthworm serine protease(ESP),and polyphenols,and detail the antitumor,antithrombotic,antiviral,antibacterial,anti-i nflammatory,analgesic,antioxidant,wound-healing,antifibrotic,and hypoglycemic activities and mechanisms of action of EE based on existing in vitro and in vivo studies.We further propose the potential of EE for clinical translation in anticancer and lipid-modifying therapies,and its promise as source of a novel agent for wound healing and resistance to antibiotic tolerance.The earthworm enzyme lumbrokinase embodies highly effective anticoagulant and thrombolytic properties and has the advantage of not causing bleeding phenomena due to hyperfibrinolysis.Its antifibrotic properties can reduce the excessive accumulation of extracellular matrix.The glycolipoprotein extract G-90 can effectively scavenge reactive oxygen groups and protect cellular tissues from oxidative damage.Earthworms have evolved a well-developed defense mechanism to fight against microbial infections,and the bioactive agents in EE have shown good antibacterial,fungal,and viral properties in in vitro and in vivo experiments and can alleviate inflammatory responses caused by infections,effectively reducing pain.Recent studies have also highlighted the role of EE in lowering blood glucose.EE shows high medicinal value and is expected to be a source of many bioactive compounds.

Effects of Nine Kinds of Household Vase Solutions on Fresh-keeping Effect of Lilium Cut Flowers

[Objective]The paper was to study the effects of nine kinds of household vase solutions on fresh-keeping effect of Lilium cut flowers.[Method]Using Lilium"Sorbonne"as the material,nine kinds of household vase solutions were prepared by edible white granulated sugar,white vinegar,akafen powder and vitamin C tablets,respectively.The morphological and physiological indexes of Lilium cut flowers,including vase life,ornamental grade,flower diameter,fresh weight of flower branches,water balance value,soluble protein content and MDA content of petals,were observed,and the effects of nine kinds of household vase solutions on fresh-keeping effect of Lilium cut flowers were studied.[Result]The household vase solution F8(20 g/L white granated sugar+1 packet/L acafen powder+2 tablets/L vitamin C tablets)had the best fresh-keeping effect on Lilium cut flowers,which was beneficial to prolong the vase life and improve the ornamental value of Lilium cut flowers.[Conclusion]The household vase solution of Lilium cut flowers is optimized,which is not only environmentally friendly but also convenient for ordinary families.

Acute Toxicity of Jinchuan Formula Plum Wine Extract and Its Protective Effect on Mice with Liver Injury

[Objectives]To investigate the acute toxicity and hepatoprotective effect of Jinchuan formula plum wine extract on mice,determine its safety range,and evaluate its hepatoprotective effect.[Methods]The median lethal dose(LD_(50))was determined by acute toxicity test with the toxic reaction and mortality of mice as indexes.Sixty Kunming mice were randomly divided into 6 groups:normal control group,model group(ConA-induced liver injury model),Jinchuan formula plum wine high,medium and low dose groups(1.0,0.5,0.25 g/kg)and silybin group(0.1 g/kg).The levels of ALT,AST,LDH in serum and TG,VLDL in liver were measured.After HE staining,the pathological changes of liver tissue in mice were observed,and the liver protective effect of Jinchuan formula plum wine extract was analyzed and evaluated.[Results]LD_(50)was 11.18 g/kg,and the 95%confidence limit of LD_(50)was 10.31-12.05 g/kg.The high-dose group of Jinchuan formula plum wine extract could significantly reduce the serum ALT and AST activities of ConA-induced liver injury mice(P<0.05).[Conclusions]Jinchuan formula plum wine extract is relatively safe,and also has a protective effect on liver injury.

Fresh-keeping Effects of Different Concentrations of 6-BA and B9 on Carnation Cut Flower

Using sucrose, citric acid and vitamin C as the basic liquid, different concentrations of 6-BA and B9 preservatives were added, and the carnation variety ＇ Master＇ was treated. The results showed that the formula of 3% sucrose ＋ 50 mg/L citric acid ＋ 600 mg/L vitamin C ＋ 20 mg/L 6-BA could effectively maintain the water balance of the cut flower, prolong the vase life of the cut flower to 16.4 d, and maximize the flower diameter and the crown height.

Protective effect of Solanum Nigrum Linn green fruit ethanolic extract on alcoholic liver injury in mice

Alcoholic liver injury is a liver disease caused by excessive alcohol consumption,which can lead to chronic liver disease death.Solanum Nigrum Linn taste bitter,cold,has the effect of clearing heat and detoxification,promoting blood and detumescence.Solanum Nigrum Linn fruit contains a variety of antioxidant enzymes,can remove the body produced by aerobic metabolism harmful substances.In this paper,a model of alcohol-induced liver injury in C57BL/6 mice was established to evaluate the protective effect of Solanum Nigrum Linn green fruit(SNGF)ethanolic extract on alcohol-induced liver injury.H&E staining and oil red O(ORO)staining showed that hepatic lobules were clearly demarcated,vacuoles were significantly reduced and lipid droplets were reduced in SNGF ethanolic extract treatment group.Serum levels of TC,TG,LDH,TBA,AKP,ALT and AST were decreased in the SNGF ethanolic extract treatment group,and SNGF ethanolic extract could clear reactive oxygen species(ROS)in time.MDA content was signifi cantly decreased after SNGF ethanolic extract treatment,while superoxide dismutase(SOD)and GSH-Px contents were increased after SNGF ethanolic extract treatment.These results suggest that SNGF ethanolic extract has a protective effect on alcohol-induced liver injury.

Study on the Antimicrobial Effect of the Extracts of Clerodendron bungei Roots

[ Objective ] The aim was to research the antimicrobial effect of the extracts of Clerodendron bungei roots with different solvents on the common plant microbes. [ Method] The bacteria cake method was used to study the antimicrobial capability of Clerodendron bungei roots. [ Result ] The extracts have anti-bacterial action on Rhixoctonia cerealis V. Hoeven, Fusarium graminearum, Rhizoctonia solani and Setosphaeria turcica, especially the extract from distilled water has the strongest anti-bacterial action on Rhixoctonia cerealis V. Hoeven and Fusarium graminearum, the extract from ether has the strongest anti-bacterial action on Fusarium graminearum, the MIC all were 10 mg/ml. The extracts had different antimicrobial effect when pH value was from 5 to 8[ Conclusion] The extracts of Clerodendron bungei roots could inhibit the 4 pathogenic microorganism, and the antibacterial activity was affected by pH value greatly.

Antibacterial Effect of Extracts from Allium mongolicum Regel

[Objective] Study on antibacterial activity and stability of the ethanol extracts from Allium mongolicum Regel.[Method] Taking Allium mongolicum Regel.(AMR)as experimental material,the antibacterial and stability test,antibacterial capacity and antibacterial activity substance stability of extracts were studied.[Result] Size order of antibacterial effect of extracts on these 5 strains tended to be:Saccharomyces cerevisiae > Penicillium sp.> Aspergillusniger> Staphylococcusaureus> Escherichia coli;under neutral or slight alkaline conditions,optimum antibacterial effect presented best;and UV light irradiation basically had no influence on tested strains.[Conclusion] Antibacterial effect of ethanol extract shows great difference among these strains,and antibacterial activity substance is of good heat and UV light stability.

Physiological Effect and Quality Influences of Hedyotis diffusae Extract on Tobacco Infected by TMV

The extract from Hedyotis diffusae was used to treat tobacco plants infected by TMV before and after incidence. The effect of the changes of some physiological and biochemical indices related with the virus-resistance in tobacco leaves on tobacco quality ware determined. The resuits showed that H. diffusae extract could remarkably increase the total phenol content in tobacco leaves ,strength the activity of phenylalanine ammonialyase （ PAL） , improve the membrane permeability and tobacco quality. Prevention treatment was better than therapy treatment. In conclusion, H. diffusae extract could induce plants to produce disease resistance and strength tha antiviral capability of tobacco against TMV.

Study on Scavenging and Disconnecting Effects of Nitrosation Reaction by Extracts of Tetradium Ruticarpum and Glycyrrhizae

In order to investigate and compare the effects of Tetradium ruticarpum and Glycyrrhizae extracts in terms of scavenging sodium nitrite and inhibiting N-ni- troso compounds formation, the 1_9（3^3） orthogonal test was conducted to extract the active components. In addition, the capacities of scavenging sodium nitrite and of in- hibiting the N-nitroso compounds formation were measured to confirm the optimum condition of extracting. When the scavenging rate was considered as the main index, the maximum capacities of scavenging sodium nitrite by the extracts of Tetradi- urn ruticarpum and Glycyrrhizae were 0.836 5 and 0.558 0, respectively. Further- more, when the disconnection rate was considered as the main index, the maximum capacities of inhibiting the N-nitroso compounds formation by the extracts of Tetradi- urn ruticarpum and G/ycyrrhizae were 0.922 8 and 0.625 6, respectively. As a re- suit, the capacity of inhibiting nitrosation reaction by Tetradium ruticarpum was shown to be much stronger than G/ycyrrhizae, no matter the scavenging rate or the disconnection rate as the main index to be considered.

Inhibitory Effects of Extracts from Marigold(Tagetes patula) against Tomato Fusarium Wilt

[ Objective ] The study aimed to explore a new way for the control of Tomato Fusarium Wilt. [ Method ] Different solvents were used to prepare the ex-tracts of marigold, and the inhibitory effects of different extraction solvents and different extraction parts of marigold against Tomato Fusar/um Wilt were compared. [ Result ] Among different solvent extracts of marigold, chloroform extracts had the strongest inhibitory effects against the growth of the pathogen; among the chloro- form extracts from different parts of marigold, root extract had the most obvious inhibitory effect against the disease, followed by flower and leaf extracts, and the in- hibitory effect of stem extract was the weakest. [ Conclusion ] The active components of marigold have inhibitory effect against Tomato Fusarium Wilt, and the plant has good development prospects and application value.

Antidiabetic and haematological effect of aqueous extract of stem bark of Afzelia africana(Smith) on streptozotocin-induced diabetic Wistar rats

Objective:To investigate the antidiabetic properties of aqueous extract of stem bark of Afzelia africana(A.africana)and its beneficial effect on haematological parameters in streptozotocin induced diabetic rats.Methods:A total of 30 rats including 24 diabetic and 6 normal rats were used for this study.Diabetes was induced in male Wistar rats by intraperitoneal injection of streptozotocin.After being confirmed diabetic,animals were orally treated with distilled water or extracts at 100 or 200 mg/kg body weight daily for 10 days.The haematological parameters including red blood and white blood cells and their functional indices were evaluated in diabetic treated groups compared with the controls.Results:The extract significantly reduced the blood glucose levels while the best result was obtained at 200 mg/kg body weight The feed and water intake in diabetic rats were significantly reduced while weight loss was minimized at both dosages.Similarly,the levels of red blood,white blood cells and their functional indices were significantly improved after extract administration at both doses.Conclusions:It can be concluded that the aqueous extract of bark of A.africana possesses antihyperglycemic properties.In addition,the extract can prevent various complications of diabetes and improve some haematological parameters.Further experimental investigation is needed to exploit its relevant therapeutic effect to substantiate its ethnomedicinal usage.

Synergistic effects of plant extracts and antibiotics on Staphylococcus aureus strains isolated from clinical specimens

Objective:This study has been done to evaluate the interaction between water extracts of Psidium guajava, Rosmarinus officinalis,Salvia fruticosa,Majorana syriaca,Ocimum basilucum,Syzygium aromaticum,Laurus nobilis,and Rosa damascena alone and then synergy testing of these extracts with known antimicrobial agents including oxytetracycline HCl,gentamicin sulfate,penicillin G,cephalexin and enrofloxacin.This study was conducted against five S.aureus isolates;one is Methicillin -resistant Staphylococcus aureus(MRSA) and 4 Methicillin - sensitive Staphylococcus aureus(MSSA).Methods:Evaluation of the interaction between plant extracts and different antimicrobial agents has been done using well - diffusion and microdilution methods. Results:The results of the conducted experiments using well - diffusion method demonstrate that these plants showed in vitro interactions between antimicrobial agents and plant extracts were additive,while using microdilution method showed synergistic effects with significant reduction in the MICs of the test antibiotics against these strains of S.aureus.This change in MIC was noticed in all plant extracts against test antibiotics including these plants showed weak antibacterial activity by well diffusion method.Synergism effect was occurred in both sensitive and resistant strains but the magnitude of minimum fold reduction of inhibitory concentration in resistant strains especially MRSA strain was higher than the sensitive strains.Coclusion:This study probably suggests the possibility of concurrent use of these antimicrobial drugs and plant extracts in combination in treating infections caused by S.aureus strains or at least the concomitant administration may not impair the antimicrobial activity of these antibiotics.

Protective effects of organic extracts of Alpinia oxyphylla against hydrogen peroxide-induced cvtotoxicitv in PC12 cells

Alpinia oxyphylla,a traditional herb,is widely used for its neuroprotective,antioxidant and memory-improving effects.However,the neuroprotective mechanisms of action of its active ingredients are unclear.In this study,we investigated the neuroprotective effects of various organic extracts of Alpinia oxyphylla on PC12 cells exposed to hydrogen peroxide-induced oxidative injury in vitro.Alpinia oxyphylla was extracted three times with 95%ethanol(representing extracts 1–3).The third 95%ethanol extract was dried and resuspended in water,and then extracted successively with petroleum ether,ethyl acetate and n-butanol(representing extracts 4–6).The cell counting kit-8 assay and microscopy were used to evaluate cell viability and observe the morphology of PC12 cells.The protective effect of the three ethanol extracts(at tested concentrations of 50,100 and 200μg/mL)against cytotoxicity to PC12 cells increased in a concentration-dependent manner.The ethyl acetate,petroleum ether and n-butanol extracts(each tested at 100,150 and 200μg/mL)had neuroprotective effects as well.The optimum effective concentration ranged from 50–200μg/mL,and the protective effect of the ethyl acetate extract was comparatively robust.These results demonstrate that organic extracts of Alpinia oxyphylla protect PC12 cells against apoptosis induced by hydrogen peroxide.Our findings should help identify the bioactive neuroprotective components in Alpinia oxyphylla.

Allelopathic Effects of Aqueous Extracts of Different Tissues and Rhizosphere Root of Adzuki Bean on Growth of Wheat Seedlings

Seedlings of wheat were used as receptor plants to study the allelopathic effects of Adzuki Bean aqueous extracts from different organs and rhizosphere soil at different concentrations （0, 10, 40 and 160 g/L） using sand culture of pot method. The results indicated that root aqueous extract of adzuki bean presented promotion effects at low concentrations and inhibition effects at the high concentra- tion on the growth of height and young leaf length of wheat seedlings, but inhibition effects at low concentrations and promotion effects at the high concentration on the growth of root length, the number of lateral roots and dry weigh of underground part of wheat seedlings. With the concentrations of rhizosphere soil, root, stem and leaf aqueous extracts of adzuki bean increasing, their inhibitory allelopathic effects on the growth of height and young leaf of wheat seedlings increased; the physiological parameters of wheat seedlings studied at the low concentrations of aqueous extracts, indicated that root and rhizosphere soil aqueous extracts of adzuki bean improved the activities of hyperoxid dismutase （SOD） and peroxide enzyme（POD）, but reduced the contents of chlorophyll and malonaldehyde （MDA）; the physiological parameters of wheat seedlings studied at the high concentration of aqueous extracts indicated that the leaf aqueous extract of adzuki bean inhibited root vigor, and reduced the contents of Chlorophyll and MDA, but it was found that the aqueous extract significantly improved the activities of SOD and POD; and as to the synthetic allelopathic effect （SE）, the allelopathic effects of different tissues and rhizosphere soil of adzuki bean on wheat seedlings were in order of leaf〉stem〉soil〉root.

Allelopathic Effects of Aqueous Extract of Torreya fargesii Aril on Seed Germination and Seedling Growth of Radish, Mustard and Cabbage

[Objective] This study aimed to investigate the allelopathic effects of Torreya fargesii aril. [Method] By indoor bioassay, the effects of different con-centrations （10, 20,40, 80 g/L） of aqueous extract of T. fargesii aril on seed germination rate, seedling root length, seedling height, fresh weight, chlorophyll content, malondialdehyde （MDA） content and anti oxidative enzyme activities of radish, mustard and cabbage were analyzed and compared. [ Result] Different concentrations of aqueous extract of T. fargesii aril inhibited seed germination and seedling growth of three crops, and the inhibitory effects were enhanced with the increase of concentration. Seedling height and fresh weight of three crops were improved by low concentrations of aqueous extract of T. fargesii aril and inhibited by high concentrations of aqueous extract. Overall, aqueous extract of T. fargesii aril exhibited allelopathic inhibitory effects on three crops, and the level of allelopath-ic inhibitory effects demonstrated a descending order of radish 〉 mustard 〉 cabbage. Compared with the control group, 80 g/L aqueous extract of T. fargesii aril almost significantly reduced seed germination rate, seedling root length, seedling height, fresh weight and chlorophyll content, and significantly improved MDA con-tent and antioxidative enzyme （SOD, CAT, POD） activities of radish, mustard and cabbage （P 〈 0 .0 5 ） . [ Conclusion] Aqueous extract of T. fargesii aril exhibited remarkable allelopathic inhibitory effects on seed germination of radish, mustard and cabbage, which indicated that there might be dormancy-associated germination in-hibiting allelochemicals in T. fargesii aril. This study provided theoretical basis for subsequent clarification of the mechanism of seed dormancy of T. fargesii.

Effects of Ginkgo biloba extract EGb761 on neural differentiation of stem cells offer new hope for neurological disease treatment

Stem cell transplantation has brought new hope for the treatment of neurological diseases.The key to stem cell therapy lies in inducing the specific differentiation of stem cells into nerve cells.Because the differentiation of stem cells in vitro and in vivo is affected by multiple factors,the final differentiation outcome is strongly associated with the microenvironment in which the stem cells are located.Accordingly,the optimal microenvironment for inducing stem cell differentiation is a hot topic.EGb761 is extracted from the leaves of the Ginkgo biloba tree.It is used worldwide and is becoming one of the focuses of stem cell research.Studies have shown that EGb761 can antagonize oxygen free radicals,stabilize cell membranes,promote neurogenesis and synaptogenesis,increase the level of brain-derived neurotrophic factors,and replicate the environment required during the differentiation of stem cells into nerve cells.This offers the possibility of using EGb761 to induce the differentiation of stem cells,facilitating stem cell transplantation.To provide a comprehensive reference for the future application of EGb761 in stem cell therapy,we reviewed studies investigating the influence of EGb761 on stem cells.These started with the composition and neuropharmacology of EGb761,and eventually led to the finding that EGb761 and some of its important components play important roles in the differentiation of stem cells and the protection of a beneficial microenvironment for stem cell transplantation.

Reducing oxidative stress and hepatoprotective effect of water extracts from Pu-erh tea on rats with high-fat diet

Reducing oxidative stress and hepatoprotective effect of Pu-erh tea water extracts on rats fed with high-fat diet were researched for explaining health care of Pu-erh tea.Fifty SD rats were divided into five groups.The body weight was measured once a day.The malondialdehyde(MDA)and glucose(Glu)levels and the activities of alanine aminotransferase(ALT),aspartate aminotransferase(AST),nitric oxide synthase(NOS),and pyruvate kinase(PK)in serum were determined.Furthermore,the hepatic glycogen level(HGL)and the activities of hepatic total superoxide dismutase(T-SOD),catalase(CAT),and glutathione peroxidase(GSH-Px)were also measured after continuous administration for 12 weeks.The result demonstrated that Pu-erh extract caused the decreases in body weight,fat index,MDA and NOS levels,and the increases in hepatic T-SOD,CAT and GSH-Px activities,indicating that the extract may be due to inhibiting the increases of body weight and fat index,reducing oxidant stress state and inhibiting lipid peroxidation,thus decreasing the activities of ALT and AST,and protecting the liver in rat.Meanwhile,the extracts could increase the production of hepatic glycogen and the activity of PK,and reduce glucose level,protecting the liver from the diseases associated with type II diabetes.

Bee pollen extract of Malaysian stingless bee enhances the effect of cisplatin on breast cancer cell lines

Objective: To evaluate the antioxidant and antiproliferative effect of methanolic bee pollen extract(BPE) of Malaysian stingless bee [Lepidotrigona terminata(L. terminata)]and its synergistic effect with cisplatin(a chemotherapeutic drug) on MCF-7 cancer cell line.Methods: The antioxidant activity of BPE from L. terminata was measured by using1,1-diphenyl-2-picrylhydrazyl radical(DPPH) assay. Antiproliferative activity at different concentrations of BPE and cisplatin was determined through using MTT assay on MCF-7 and L929 cell lines. An interaction effect(synergistic, additive and antagonistic) between BPE and cisplatin was determined by Compu Syn software based on MTT assay data.Results: The EC50(50% decrement of DPPH inhibition) of BPE was 0.5 mg/m L.L. terminata BPE exhibited antiproliferative activity on both cancer and normal cell lines.The IC50(concentration of drug that was required for 50% of cell inhibition in vitro) of BPE on MCF-7 was 15 mg/m L whereas in normal cell line L929 was 26 mg/m L. The IC50 for cisplatin on MCF-7 was 20 mmol/L. The combination effect of BPE and cisplatin on MCF-7 cells showed that BPE at 15 mg/m L was able to potentiate the inhibitory effect of cisplatin at all different concentrations(2.5–20.0 mg/m L). The average of cancer cells inhibition which was potentiated by BPE was around 50%. A combination index values of less than 1 reported in the Compu Syn software further proved the synergistic effect between BPE and cisplatin, suggesting that BPE was working synergistically with cisplatin.Conclusions: Our study therefore suggested that BPE of Malaysian stingless bee,L. terminata is a potential chemopreventive agent and can be used as a supplementary treatment for chemotherapy drugs. BPE might be able to be used to potentiate the effect of chemotherapy drugs with the possibility to reduce the required dose of the drugs. The molecular mechanisms of how the BPE exerts antiproliferative activity will be a much interesting area to look for in future studies.

The Impact on Extraction Effect of Polysaccharide from Ganoderma lucidum by Different Pretreatment

[Objectives] The research aimed to study the impact on extraction effect of polysaccharide from Ganoderma lucidum( lingzhi) by different pretreatment methods. [Methods] The impacts on extraction of G. lucidum polysaccharide by soaking,microwave and air flow fine pulverization were contrasted,and the extraction effect of G. lucidum polysaccharide by combining the optimal pretreatment manner with hot water extraction,alcohol extraction,alkali extraction,ultrasonic binding enzyme extraction,and microwave extraction was compared. Finally,the property of G. lucidum polysaccharide obtained after air flow fine pulverization pretreatment was detected and analyzed by high performance liquid chromatography. [Results] The optimal pretreatment method was air flow fine pulverization. Compared with traditional method-direct extraction( coarse grinding combining hot water extraction),crude yield changed little,while polysaccharide content and yield were improved by 114% and 104%. The best combination manner was air flow fine pulverization pretreatment combining with alkali extraction. Compared with traditional method,crude yield,polysaccharide content and yield were improved by 76%,78% and 215% respectively. The property of G. lucidum polysaccharide obtained after air flow fine pulverization pretreatment was detected and analyzed by high performance liquid chromatography. It was found that the treatment method had little impact on the property of G. lucidum polysaccharide. [Conclusions]Air flow fine pulverization pretreatment could greatly improve extraction effect of G. lucidum polysaccharide,which mainly improved the content and yield of G. lucidum polysaccharide,and extraction was more complete,with less impact on the property of the extracted polysaccharide. It was speculated that air flow fine pulverization pretreatment mainly destroyed mechanical support wall membrane structure of G. lucidum fine powder,making that intracellular functional substances completely dissolved out of the cell,and the content would be studied in follow-up experiment.