Antisepsis and Fresh-keeping Effects of Natamycin Coating Compounds Treatment on Red Global Grape

The aim was to research fresh-keeping effects of natamycin on cold-pre- served grape. Red globe grapes were processed with compound coating liquid of chitosan with mass fraction at 1% and natamycin with mass fractions at 0.20% （T1）, 0.40% （T2） and 0.60% （T3）, respectively. Grapes processed with water （CK3） and 1% chitosan （CK2） were taken as control groups. Rotten rate, seed shattering rate, mass loss rate, respiratory intensity and related physiological quality in test and control groups were compared. The results indicated that respiratory intensity, mass loss rate, rotten rate and seed shattering rate in CK1 were all higher than those in CK2. In addition, T1, T2 and T3 were lower in the indices than CK1 and CK2, but still kept at a high level in fruit hardness. Furthermore, mass fractions of Vc and titratable acid declined more slowly in T1, T2 and T3, compared with CK1 and CK2. Natamycin better preserved grapes and prolonged storage period. In general, natamycin with mass fraction at 0.4% proved best in fresh-keeping.

Relationships of growth and mortality to enzymatic activity,and the relative mRNA expression of cultured scallops Patinopecten yessoensis in the Yellow Sea, China

The bottom aquaculture of yesso scallop (Patinopecten yessoensis) has developed rapidly in the north of Yellow Sea, China, but not without accompanying productivity problems. We, therefore, conducted field surveys to investigate factors related to growth and mortality rates of bottom-cultured scallops after release. Specifically, we focused on the effects of total antioxidant capacity (TAOC) and three key enzymes involved in antioxidant and metabolic function:glutamic pyruvic transaminase (GPT), lactic dehydrogenase (LDH), and superoxide dismutase (SOD). Across summer and winter, we also measured the relative mRNA expression of GPT and SOD to understand their seasonal variation in the scallops, along with how such variation correlated to growth and mortality. Results show that bottom cultured scallops experienced mass mortality during the first six months post-release. During winter (December), scallops grew more rapidly and suffered less mortality than in summer (August). The observed lower performance probably resulted from less advantageous environmental factors during the summer seasons, such as high temperature and low dissolved oxygen. These environmental stressors enhance protein consumption while decreasing energetic resources in scallops. Furthermore, scallops in summer exhibited high antioxidant levels that probably competed for energy with process integral to growth and survival. These negative factors combined to elevate mortality rates. In conclusion, we provided evidence suggesting correlations between metabolic/antioxidant activity and growth and mortality of bottom-cultured yesso scallops. These correlations implied us an accurate method to estimate the performance of bottom culture system. Suggestions about innovative aquaculture techniques were also discussed in the study. Our results might provide a possible guideline to the improvement of bottom culture techniques for this commercially valuable seafood species.

Yield trait improvement of bay scallops following complete diallel crosses between different scallop stocks

This study describes the complete diaUel hybridization between newly introduced bay scallop stock （W） from Canada and local commercial stock （D） grown under laboratory conditions, in China. Larval survival and growth during all life stages （larvae, spat, and adult） were compared among hybrid （DW, WD） and purebred （DD, WW） populations. Significant heterosis was detected for survival during the larval stage （〉 1% of the mid-parent values）. The mean heterosis （Hm） varied in growth throughout the life span. More than 50% of the Hm values were positive and negative in the DW and WD groups, respectively. The influence of maternal effects and mating types （intrapopulation vs. interpopulation crosses） on growth for all life stages was not consistent. Larval survival did not differ significantly （P〉0.05） with maternal effect or mating type. In the harvest stage, shell length （SL）, shell height （SH）, shell width （SW）, and total weight （TW） were larger in the hybrid compared with the inbred groups. Positive Hm values were observed in SL （1.5%）, SW （5.8%）, and TW （12.3%）, and were more significant in the DW groups （6.1%, 4.5%, 6.8%, and 27.2%）. These results suggest that hybridization between two geographic populations is a good tool for improving bay scallop growth. However, unstable heterosis between the two populations requires further study.

Effects of Nine Kinds of Household Vase Solutions on Fresh-keeping Effect of Lilium Cut Flowers

[Objective]The paper was to study the effects of nine kinds of household vase solutions on fresh-keeping effect of Lilium cut flowers.[Method]Using Lilium"Sorbonne"as the material,nine kinds of household vase solutions were prepared by edible white granulated sugar,white vinegar,akafen powder and vitamin C tablets,respectively.The morphological and physiological indexes of Lilium cut flowers,including vase life,ornamental grade,flower diameter,fresh weight of flower branches,water balance value,soluble protein content and MDA content of petals,were observed,and the effects of nine kinds of household vase solutions on fresh-keeping effect of Lilium cut flowers were studied.[Result]The household vase solution F8(20 g/L white granated sugar+1 packet/L acafen powder+2 tablets/L vitamin C tablets)had the best fresh-keeping effect on Lilium cut flowers,which was beneficial to prolong the vase life and improve the ornamental value of Lilium cut flowers.[Conclusion]The household vase solution of Lilium cut flowers is optimized,which is not only environmentally friendly but also convenient for ordinary families.

Fresh-keeping Effects of Different Concentrations of 6-BA and B9 on Carnation Cut Flower

Using sucrose, citric acid and vitamin C as the basic liquid, different concentrations of 6-BA and B9 preservatives were added, and the carnation variety ＇ Master＇ was treated. The results showed that the formula of 3% sucrose ＋ 50 mg/L citric acid ＋ 600 mg/L vitamin C ＋ 20 mg/L 6-BA could effectively maintain the water balance of the cut flower, prolong the vase life of the cut flower to 16.4 d, and maximize the flower diameter and the crown height.

Fossil Scallops from the Cenozoic Duho Formation of the Pohang Basin,Korea

A great number of fossil scallops were found from the Duho Formation of the Pohang Basin,Korea.Most of the specimens are preserved as slightly altered original carbonate shells,and internal and external molds were also observed in the specimens whose calcareous materials were washed out.In particular, external and internal molds are more frequently observed from the thin and small(less than 1 cm

Characterization of Wnt genes in Argopecten scallops and their involvement in response to different temperature stresses in“Bohai Red”scallops

As“Bohai Red”scallops were originated from the hybrids between the Peruvian scallop(Argopecten purpuratus)and the bay scallop(Argopecten irradians)northern subspecies(Argopecten irradians irradians).Twelve Wnt members were identified from the two subspecies of bay scallop,and 13 Wnt genes were found in the genome of the Peruvian scallop.Protein structure analyses showed that most Wnt genes poses all 5 conserved motifs except Wnt1,Wnt2,Wnt6,and Wnt9 in the bay scallops and Wnt2 and Wnt9 in the Peruvian scallop.Unexpectedly,Wnt8 gene was present while Wnt3 was absent in both the bay scallops and the Peruvian scallop.Phylogenetic analysis revealed that Wnt3 might have disappeared in the early evolution of mollusks.The expression profile of Wnt genes in the“Bohai Red”exposed to different temperatures was examined by qRT-PCR.Results show that expression of Wnt genes responded differentially to temperature changes.The Wnt genes such as Wnt1,Wnt6,Wnt7,Wnt11,and WntA that responded slowly to low and high temperature stresses may be related to the maintenance of basic homeostasis.Other Wnt genes such as Wnt4,Wnt9,Wnt5,and Wnt2 that responded rapidly to low temperature may play an important role in organismal protection against low temperature stress.And yet some Wnt genes including Wnt10,Wnt16,and Wnt8 that responded quickly to high temperature stress may play key roles in response to high temperature stress.The results provide new insights into the evolution and function of Wnt genes in bivalves and eventually benefit culture of“Bohai Red”scallops.

The phylogeny of native and exotic scallops cultured in China based on 16S rDNA sequences

Scallops of the Family Pectinidae are a valuable resource in marine industry of the world. Understanding the phylogeny of the family is important for the development of the industry. In this study, partial 16S mitochondrial rDNA genes were obtained from 8 scallop species that are commonly cultured indigenous and transplanted species in China. Phylogenetic relationships of Pectinidae were analyzed based on the 8 sequences and other 5 published ones in GenBank, representing 9 genera of the family. The molecular phylogeny trees were constructed using 3 methods with software PHYLIP. The results showe that total 13 species of scallops clustered in 4 clades. Pecten maximus joins P. jacobaeus then Amusium pleuronectes in cluster, indicating close relationship of genus Amusium with Pecten in evolution. P. yes- soensis is close to Chlamys farreri and C. islandica. No enough material was available to single out genus Patinopecten as an independent monophyletic subfamily. The position of Adamussium colbecki indicates that it is far from genus Pecten but near to genus Chlamys in evolution.

Kiwi Fruit Fresh-keeping Technology

The vitamin C rich-kiwi fruit-the king of fruits-is good to taste but hard to store. Only a few countries can prevent mildew on the fruit stalk during the storage process. The fresh-keeping technology developed by the Shaanxi Teachers University has enabled China to catch up with the advanced countries in this field.

The suitability assessment on site selection for bottom seeding scallop culture based on analytic hierarchy process

Scallop culture is an important way of bottom-seeding marine ranching,which is of great significance to improve the current situation of fishery resources.However,there are some problems in site-selection evaluation of marine ranching,such as imperfect criteria system,complex structure,untargeted criteria quantification,etc.In addition,no site-selection evaluation method of bottom-seeding culture areas for scallops is available.Therefore,we established a hierarchy structure model according to the analytic hierarchy process(AHP)theory,in which social,physical,chemical,and biological environments are used as main criteria,and marine functional zonation,water depth,current,water temperature,salinity,substrate type,water quality,sediment quality,red tide,phytoplankton,and zooplankton are used as sub-criteria,on which a multi-parameter evaluation system is set up.Meanwhile,the dualism method,assignment method,and membership function method were used to quantify sub-criteria,and a quantitative evaluation for the entire criteria was added,including the evaluation and analysis of two types of unsuitable environmental situations.By overall consideration in scallop yield,quality,and marine ranching construction objectives,the weight of the main criteria could be determined.Five grades in the suitability corresponding to the evaluation result were divided,and the Python language was used to create an evaluation system for efficient calculation and intuitive presentation of the evaluation outcome.Eight marine cases were simulated based on existing survey data,and the results prove that the method is feasible for evaluating and analyzing the site selection of bottom-seeding culture areas for scallops under various environmental situations.The proposed evaluation method can be promoted for the site selection of bottom-seeding marine ranching.This study provided theoretical and methodological references for the site selection evaluation of other types of marine ranching.

ScanSAR的Scalloping辐射误差研究

由于在观测带内采用了多波束扫描的工作模式,使得ScanSAR产生了固有的周期性的scalloping效应,影响了ScanSAR的辐射特性。该文基于ScanSAR工作模式的特点,研究了scalloping产生的机理和性质,定量地分析了scalloping引起的辐射误差以及多普勒中心误差与scalloping的关系,并研究了对scalloping进行校正后的剩余误差问题。

Effect of Fouling in Daya Bay Scallop chlamys nobilis (reeve) Cultivation:Food Competition and Nutrient Release

Biofouling is an important factor that affects bivalve farming industry. Fouling organisms may reduce growth and survival rate of the cultured species. Fouler are often filter feeders, so they are potential competitors for food resource with the cultured species. The present study was conducted to measure the impact of fouling on food uptake and nutrient release in April, 2006 in Daya Bay near Guangzhou, China. Results showed that fouling organisms had significant effect on food uptake and nutrient release. The chlorophyll a uptake rate of fouled scallops was 7.53 / Lh ±1.416 / Lh in April, significantly higher than those of cleaned scallops, Le., 4.23 / Lh±2.744 / Lh. The consumption of total particulate matter by fouled scallops in April was 5.52 / Lh± 0.818 / Lh; the corresponding results for cleaned scallops are 2.49 / Lh ±0.614 / Lh Fouling increased ammonia release significantly. The ammonia release rate of fouled scallops was 33.81/Lh ±7.699 / Lh in April, while cleaned scallops released 2.46 / Lh ±0.511 / Lh ammonia. Phosphate uptake of fouled scallops was 2.01± 0.699 / Lh and cleaned scallops released phosphate 6.01 / Lh ＋ 0.876 / Lh in April. There was not significant difference in nitrate consumption between fouled and cleaned scallops. According to the phytoplankton classification of input and output water samples, fouled scallops filtered more phytoplankton species than cleaned scallops. Therefore, this study showed that fouling contributed much to phytoplankton depletion and concentration increase of ammonia in water.

墨西哥湾扇贝杂交选育系F_(2)代的现实遗传力

为评估墨西哥湾扇贝野生群体与扇贝“渤海红”正交后代黑白壳色速生选育系育种效果,在正交一代中采用黑白壳色为首选,5%最大壳长为二选的育种策略,获得选育系F_1,再以相同方法获得“选F_(2)”。以F_1自繁获得的“非选F_(2)”为对照,研究“选F_(2)”当代遗传获得及各经济性状现实遗传力。试验结果显示:“选F_(2)”存活率相较于“非选F_(2)”无显著性差异,但“选F_(2)”各生长性状均显著优于“非选F_(2)”(P<0.05),其壳长、壳高、壳宽、体质量、闭壳肌质量和软体质量当代遗传获得分别为8.29%、6.65%、10.26%、20.72%、33.98%和25.91%,变异系数分别为7.01%、8.46%、8.50%、14.80%、26.32%和15.98%;选育系各生长性状现实遗传力为0.41~0.47,均属高等遗传力。试验结果表明,对杂交选育F_1代开展壳长选育可带来显著的遗传进展,选育效果显著,且F_(2)代仍有较大继续选育的潜力,试验结果为墨西哥湾扇贝选育系的进一步选育提供基础数据。

Removal of biomass tar by steam reforming over calcined scallop shell supported Cu catalysts

In this study, the main purpose is to develop low-cost catalysts with high activity and stability for high quality syngas production via steam reforming of biomass tar in biomass gasification process. The calcined waste scallop shell（CS） supported copper（Cu） catalysts are prepared for steam reforming of biomass tar. The prepared Cu supported on CS catalysts exhibit higher catalytic activity than those on commercial CaO and Al;O;. Characterization results indicate that Cu/CS has a strong interaction between Cu and CaO in CS support, resulting in the formation of calcium copper oxide phase which could stabilize Cu species and provide new active sites for the tar reforming. In addition, the strong basicity of CS support and other inorganic elements contained in CS support could enhance the activity of Cu/CS. The addition of a small amount of Co is found to be able to stabilize the catalytic activity of Cu/CS catalysts,making them reusable after regeneration without any loss of their activities.

Effects of hypoxia on survival,behavior,and metabolism of Zhikong scallop Chlamys farreri Jones et Preston 1904

Hypoxia,a frequent occurring threat in coastal regions,often results in mass mortalities of marine organisms and brings a serious ecological problem.The commercially important Zhikong scallop Chlamys farreri is being under such a threat as the risks of eutrophication and hypoxia have risen in their culture areas.However,little information has been known concerning their tolerance to hypoxia and their strategy for survival.In the present study,a 20-day experiment was conducted to determine the ef fects of hypoxia on the survival,behavior,and metabolism of Zhikong scallop.With the LC_(50)for dissolved oxygen(DO)being estimated as 1.8 mg/L,the survival of Zhikong scallop can be greatly challenged even under the moderate hypoxic condition of around 2.0 mg/L DO.The survival rate ranged from 69%to 59%when DO dropped from 3.0 to 2.0 mg/L,and it was further reduced to 47%at 1.5 mg/L DO.In hypoxic conditions,the scallops became significantly active,which may be explained as escape attempts to avoid hypoxic water.To save energy,Zhikong scallop would depress their respiration.However,when DO dropped from 3.0 to 2.0 mg/L,the oxygen consumption rate hardly changed.The upregulation of lactate dehydrogenase activity and the unrepressed phosphofructokinase activity,which often result in the unbalanced cellular homeostasis and energy budget,may account for the observed increase in the mortality rate of Zhikong scallops.In general,Zhikong scallop is sensitive to hypoxia events,though possible escape attempts,depressed respiration,and oxaloacetate-pathway may increase their survival chance.

POLYCULTURE OF SCALLOP CHLAMYS FARRERI AND KELP LAMINRIA JAPONICA IN SUNGO BAY

Several polyculture models of scallop Chlamys farreri and kelp Laminaria japonica currently employedin Sungo Bay and other parts of northern China are described in this paper. Economic benefits of differ-ent polyculture models are analysed based on the growth rate, culture density and market price. In addi-tion, site selection, critical environmental conditions and polyculture problems are discussed in detail.

Molecular identification of scallop planktonic larvae using species-specific microsatellites

The identification of scallop larvae is essential to understand the population structure and community dynamics and to assess the potential environmental impacts caused by scallop larvae released or escaped. However, the larvae identification by morphological characteristics is notoriously difficult, mainly due to the small size （usually being less than 150 μm） and vague morphological characteristics among different scallop species. A simple and accurate molecular method was developed to identify four economically farmed scallop species, the Zhikong scallop Chlamys farreri, the noble scallop C. nobilis, the bay scallop Argopecten irradians and the Yesso scallop Mizuhopecten yessoensis. The tests used the high degree of species-specific microsatellite markers, which was specified by transferability analyses, assessed by reference individuals and evaluated by BLAST searches. The sensitivity test indicated that the species-specific microsatellites were sensitive enough for the detection of 1% -2% larvae in mixed plankton samples. Larvae collected from scallop hatcheries and their effluents and from the artificially controlled crosses were well identified to the species/hybrid level. The results demonstrated that the one-step PCR-based assay was technically simple, inexpensive and robust in identification analyses, and also less sensitive to initial quality of template DNA extracted from the ethanol-preserved samples for several years.

Genetic diversity and specific markers in four scallop species, Patinopecten yessoensis, Argopecten irradians, Chlamys nobilis and C.farreri

The AFLP （amplified fragrnent length polymorphism）technique was used to analyse the genetic diversity in four scallop species, Patinopecten yessoensis, A rgopecten irradians, Chlamys nobilis and C. farreri. The genetic similarity indexes of these four species are 0.8415, 0.7863, 0.7190 and 0.6731, while Shannon diversity indexes are 43.52, 58.87, 80.16 and 92.83, respectively. As analyzed, the genetic diversities in two native species, i.e., C, farreri and C. nobilis, are higher than those in other two introduced species, A. irradians and P. yessoensis. The results also showed that C. nobilis and C, farreri shared the most common loci. The genetic distance indicated that C. nobilis and C. farreri are closely related. Moreover, out of 510 AFLP markers, 21 specific bands are found to distinguish the four species scallops and these markers may be applied to the specific germplasm characterization and molecular assistant classification in scallops.

Effects of scallop shell extract on scopolamine-induced memory impairment and MK801-induced locomotor activity

Objective:To evaluate the neuroprotective effects of the organic components of scallop shells(scallop shell extract) on memory impairment and locomotor activity induced by scopolamine or 5-methyl-10,11-dihydro-5H-dibenzo(a,d) cyclohepten-5,10-imine(MK801).Methods:Effect of the scallop shell extract on memory impairment and locomotor activity was investigated using the Y-maze test,the Morris water maze test,and the open field test.Results:Scallop shell extract significantly reduced scopolamine-induced short-term memory impairment and partially reduced scopolamine-induced spatial memory impairment in the Morris water maze test.Scallop shell extract suppressed scopolamine-induced elevation of acetylcholine esterase activity in the cerebral cortex.Treatment with scallop shell extract reversed the increase in locomotor activity induced by scopolamine.Scallop shell extract also suppressed the increase in locomotor activity induced by MK801.Conclusions:Our results provide initial evidence that scallop shell extract reduces scopolamine-induced memory impairment and suppresses MK-801-induced hyperlocomotion.

Effects of Temperature on Haemocyte and Granulocyte Counts and Expressions of Immunity-Related Genes in Haemocytes of Scallop Chlamys farreri After Vibrio anguillarum Infection

Bivalve live in aquatic environment and the water temperature can affect their immunity directly.In this research,the scallop Chlamys farreri was injected with 10^4 or 10^7 CFU mL^-1 Vibrio anguillarum and cultured at 11℃,17℃,23℃,and 28℃,respectively.For the control scallop,only phosphate-buffered saline(PBS)was injected.Then total haemocytes and granulocytes were measured by ELISA using monoclonal antibodies.In the meantime,expressions of six immunity-related genes,including lipopolysaccharide andβ-1,3-glucan binding protein(CfLGBP),C-type lectin(CfLec-2),Toll-like receptor(Cf TLR),Lysozyme(CfLYZ),superoxide dismutase(SOD),and phenoloxidase(CfPO)in haemocytes were measured using quantitative real-time PCR.The results showed that total haemocytes counts in 10^4 CFU mL^-1 injection groups showed no differences compared to the control group at all temperatures.However,they varied significantly in 10^7 CFU mL^-1 injection groups at 3 h at 11℃,6–12 h at 17℃,3–48 h at 23℃,and 12–48 h at 28℃.Granulocytes counts in 10^4 CFU mL^-1 injection groups showed no variance compared to the control group at all temperatures,except for 12 h at 23℃,and 24–36 h at 28℃.They were significantly decreased in 10^7 CFU mL^-1 injection groups during 6–48 h at 11℃,12–48 h at 17℃,3–48 h at 23℃,and 3–72 h at 28℃.The expression levels of six immunity-related genes in haemocytes of 10^7 CFU mL^-1 injection groups were significantly higher than those of control group and 10^4 CFU mL^-1 injection groups at all temperatures.The results indicated that infected with high concentration of vibrios,haemocyte counts,granulocyte counts and the expressions of immunity-related genes in scallop C.farreri were significantly affected by environmental temperature.