Effects of Different Seed Stem Sizes on the Changes of Available Elements in Rhizosphere Soil of Fritillaria thunbergii Miq.

[Objectives]This study was conducted to screen suitable seed stems of Fritillaria thunbergii Miq.from different provenances and to provide a theoretical basis for the high-yielding and high-efficiency cultivation of F.thunbergii Miq.introduced to different places.[Methods]F.thunbergii Miq.from four different provenances including Zhejiang,Nantong and Chongqing were introduced and cultivated in Wanzhou of Chongqing.The contents of available Zn,Fe,Mn,Cu,Mo,N,P,K,Ca and Mg in rhizosphere soil of F.thunbergii Miq.during five growing stages were determined after selecting different stem sizes for field cultivation.[Results]Small stems of Pan an and Ningbo provenances(SSG3,121-160/kg)and middle stems of Nantong and Fengjie provenances(SSG2,81-120/kg)showed higher soil availability.[Conclusions]In the process of introduction and cultivation of F.thunbergii Miq.,high yield and high efficiency can be achieved by selecting smaller seed stems of F.thunbergii Miq.

Current Situation of Fritillaria thunbergii Miq. Production in Yinzhou District of Ningbo City

Yinzhou District of Ningbo City is the home and main production area of Fritillaria thunbergii Miq. Based on the analysis of Fritillaria thunbergii Miq. production in Yinzhou District in 2015,this paper firstly introduced current situation of Fritillaria thunbergii Miq. planting. The competition of Fritillaria thunbergii Miq. products was in disorder,the market price in 2015 dropped to half in 2014,and many Fritillaria thunbergii Miq. growers suffered losses. To keep sustainable development of traditional Fritillaria thunbergii Miq. industry in old production areas and maintain brand and name of genuine materials of " Zhejiang 8 Famous Kinds Herbal Medicines",it is required to actively reduce the inventory,make up short board,strengthen advantages,implement the reform of supply font,keep up with the times and market,and succeed through improving the quality.

TWO NEW STEROIDAL ALKALOIDS,DONGBEININE AND DONGBEIRINE,FROM FRITILLARIA THUNBERGII MIO.VAR.CHEKIANGENSIS

Two new D/E trans-5(?)-cevanine alkaloids,Dongbernine and Dongbeirine,have been isolated from Fritillaria thunbergii Miq.var.chekiangensis together with six known compounds,peimine,peiminine,isoverticine,verticindiol,B—sitosterol daucoterol.

Temperature Effects on Seed Dormancy Relieving of Fritillaria thunbergii Miq

The seed dormancy relieving of Fritillaria thunbergii Miq required 50 days at 8～10℃and 80 days at 3～5℃ In order to study the physiological basis for seed dormancy relieving,peroxidase,acid phosphatase and proteins were assayed by spectrophotometric analysis and electrophoresis The results showed that peroxidase activity and acid phosphatase activity increased and new bands were detected in protein pattems as the seeds were treated with low temperature,these changes resulted in the seed dormancy relieving

Studies on the Chemical Constituents of Four Species of Fritillaria Plants

The bulbs of Fritillaria spp.(Liliaceae)are used in Chinese medicine as antipyretic,anti-tussive,expectorant,etc.In order to search for the bioactive constituents from this genus,thebulbs of four species of medicinal Fritillaria plants have been investigated.Thirteen compoundswere isolated from Fritillaria thunbergii Miq.,three of them were new compounds.Fourteencompounds from Fritillaria thunbergii var.chekiangensis Hsiao et K.C.Hsia collected inDongyang of Zhejiang Province,four of them are new compounds and another one was first iso-

Screening of the Optimal Potassium Fertilization Rate for Maximizing Bulb Yield and Potassium Nutrient Evaluation in Fritillaria thunbergii Miq.

Background:The dry bulb of Fritillaria thunbergii Miq.is a traditional Chinese medicine,and is the leading product of the geo-authentic crude drugs‘Zhebawei’in Zhejiang Province.Fritillaria thunbergii requires more potassium(K)than nitrogen and phosphorus,while the response of F.thunbergii to K has rarely been studied.Objective and Methods:To evaluate the K nutritional status of F.thunbergii,a 2-year field experiment was conducted under six K application rates(0,40,80,120,160,and 200 kg K_(2)O ha−1)with two F.thunbergii cultivars(ZB1,narrow-leaf;ZB2,broad-leaf).Results:The bulbus Fritillariae Thunbergii(BFT)yield increased to a plateau with more than~120 kg K_(2)O ha-1.The BFT quality met the standard of Pharmacopoeia of P.R.China with more than 40.0 kg K_(2)O ha^(-1).Leaf K concentration was significantly correlated with BFT yield,and was more sensitive to K application rate as compared to those of stems and flowers.Potassium application extended the duration of rapid leaf-biomass accumulation and increased the accumulation rate.Two cultivars had similar response patterns to K application,but ZB2 had higher K partial productivity,leaf-biomass,and leaf K concentration than ZB1.Conclusion:108.4-128.0 kg K_(2)O ha^(-1) was the optimal K application range for BFT yield.The potassium nutrition index(KNI)model established based on leaf K concentration has biological significance and can be used for dynamic diagnosis of K nutritional status of F.thunbergii with both accuracy and simplicity.