期刊文献+
共找到7篇文章
< 1 >
每页显示 20 50 100
来源于瘤胃厌氧真菌Neocallimastix frontalis木聚糖酶在毕赤酵母中的表达 被引量:6
1
作者 汪艳 李晓 +1 位作者 陈勇 武运 《生物技术通报》 CAS CSCD 北大核心 2015年第5期186-193,共8页
厌氧真菌Neocallimastix frontalis是瘤胃中降解木聚糖和纤维素的主要微生物之一,其木聚糖酶具有潜在的应用价值。对来源于Neocallimastix frontalis木聚糖酶基因Xyn11B进行密码子优化;通过全基因合成优化后的木聚糖酶基因Xyn11Bm,构建... 厌氧真菌Neocallimastix frontalis是瘤胃中降解木聚糖和纤维素的主要微生物之一,其木聚糖酶具有潜在的应用价值。对来源于Neocallimastix frontalis木聚糖酶基因Xyn11B进行密码子优化;通过全基因合成优化后的木聚糖酶基因Xyn11Bm,构建该基因的酵母表达载体p PIC9K-Xyn11Bm,并在毕赤酵母GS115中诱导表达。摇瓶水平时,重组Xyn11Bm酶活性最高为4 874.8U/m L。在10 L发酵罐中诱导96 h后,重组Xyn11Bm的酶活性为5 139.7 U/m L,菌体湿重和干重达到216.7 g/L和117.3 g/L。酶学性质分析表明,重组Xyn11Bm的最适反应温度为50℃,最适反应p H为5.0。在p H5.0-8.0时该酶具有较好的稳定性,但温度稳定性较差。底物特异性分析表明,重组Xyn11Bm可水解燕麦木聚糖、桦木木聚糖和可溶性木聚糖4-O-Me-D-glucurono-D-xylan,但不降解地衣多糖和大麦β-葡聚糖。结果表明重组Xyn11Bm具有潜在的应用价值。 展开更多
关键词 Neocallimastix frontalis 木聚糖酶 毕赤酵母 密码子优化 酶学性质
下载PDF
Biological Characteristics and Rumen Microorganisms of Gayal(Bos frontalis)in Yunnan Province 被引量:3
2
作者 杨舒黎 苟潇 +3 位作者 冷静 毛华明 邓卫东 吴锡川 《Agricultural Science & Technology》 CAS 2011年第8期1234-1237,共4页
[Objective]The paper was to provide reference for further study and development of gayal(Bos frontalis).[Method]According to the research status at home and abroad,the biological characteristics and rumen microorgan... [Objective]The paper was to provide reference for further study and development of gayal(Bos frontalis).[Method]According to the research status at home and abroad,the biological characteristics and rumen microorganisms of gayal in Yunnan Province was studied.[Result]Gayal in Yunnan had typical body form and very good meat production performance,its muscle fiber diameter was significantly less than other kinds of cattle;the water holding ratio,muscle tenderness and muscle succulency were significantly higher than others;its amount,shape and structure of chromosome were different from yellow cattle(Bos taurus)and wild cattle(Bos gaurus),and the amounts of those chromosomes(2n)were 58,60 and 56,respectively.It could create hybrid with yellow cattle;the gayal's special diet was bamboo,its in vitro dry matter digestibility(IVDMD)on various crude forage was significantly higher than yellow cattle in Yunnan;the viable bacteria and cellulolytic bacteria in rumen were 4.51×109 and 1.63×109 CFU/ml,which was significantly higher than yellow cattle in Yunnan,its dominant bacteria in rumen mainly was cellulolytic bacteria.[Conclusion]Gayal not only had high academic value,but also had a great development value. 展开更多
关键词 Gayal(Bos frontalis)in Yunnan Meat quality Genetic diversity Nutrient digestibility Rumen microorganism
下载PDF
The inflammation influence on corneal surface after frontalis suspension surgery 被引量:3
3
作者 Kang Li Xin-Chun Zhang +2 位作者 Xian-Xian Cai Ya-Dan Quan Rong Lu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2018年第9期1489-1495,共7页
AIM: To study the influence of frontalis muscle flap suspension on ocular surface by analyzing the clinical features and inflammatory cytokines.METHODS: A prospective, observational case series. Thirty-one eyes of 2... AIM: To study the influence of frontalis muscle flap suspension on ocular surface by analyzing the clinical features and inflammatory cytokines.METHODS: A prospective, observational case series. Thirty-one eyes of 25 patients with severe congenital blepharoptosis who underwent frontalis muscle flap suspension surgery with at least 6 mo of follow-up were included in the study. The main outcome measures were margin reflex distance 1(MRD_1), degree of lagophthalmos, ocular surface disease index(OSDI), fluorescein staining(Fl), tear break-up time(BUT), Schirmer I test, and inflammatory cytokine assay.RESULTS: The degrees of lagophthalmos significantly increased after surgery. The OSDI scores significantly increased 1wk postoperatively and then decreased 4wk after operation. The Fl scores reflected corneal epithelial defects in sixteen patients at early stage postoperatively. The BUT and Schirmer I test values remained stable and did not show change compared to those before surgery. The inflammatory cytokines in conjunctival epithelial cells(including IL-1β, IL-6, IL-8, TNF-α, and IL-17A) significantly increased 1wk after the surgery(P〈0.001), then returned to the normal level at 24wk postoperatively. The levels of inflammatory cytokine IL-1β, IL-6, IL-8, TNF-α, and IL-17A elevated significantly and were positively correlated with OSDI and Fl scores.CONCLUSION: Frontalis muscle flap suspension surgery results in lagophthalmos in early period of post-operation and relieved after months. The elevation of inflammatory cytokines level may participate in the occurrence of corneal epithelial defects at the early postoperative stage. 展开更多
关键词 congenital blepharoptosis frontalis muscle flap suspension corneal epithelial defects INFLAMMATION
下载PDF
Spatial Distribution Pattern of the Steppe Toad-headed Lizard(Phrynocephalus frontalis) and Its Influencing Factors 被引量:1
4
作者 Xue LIAN Zhigang JIANG +3 位作者 Xiaoge PING Songhua TANG Junhuai BI Chunwang LI 《Asian Herpetological Research》 SCIE 2012年第1期46-51,共6页
Spatial distribution patterns are associated with life history and behavioral adaptations of animals. For studying the spatial distribution pattern of the steppe toad-headed lizard(Phrynocephalus frontalis) and its in... Spatial distribution patterns are associated with life history and behavioral adaptations of animals. For studying the spatial distribution pattern of the steppe toad-headed lizard(Phrynocephalus frontalis) and its influencing factors,we conducted experiments in Hunshandake Sandy Land in Inner Mongolia,China in July 2009. By calculating the clustered indices,we found that the lizard was aggregately distributed when the sampling quadrat was smaller than 10 m × 10 m,and uniformly distributed when it was greater than 10 m × 10 m. The Nearest Neighbor Rule showed a clustering distribution pattern for P. frontalis and the distribution pattern was quadrat-sampling dependent. Furthermore,the cluster was determined by environmental factors when the sampling quadrat was smaller than 20 m × 20 m,but it was determined by both environmental factors and characteristics of the lizard when it was larger than 20 m × 20 m. Our results suggested that the steppe toad-headed lizards tended to aggregate into suitable habitat patches in desert areas. Additionally,we discussed that the lizard aggregation could be potentially used as an indictor of movement of sand dunes. 展开更多
关键词 sand lizard Phrynocephalus frontalis spatial distribution environmental factor quadrat-sampling effect
下载PDF
Severe unilateral congenital ptosis with poor levator function:tarsoconjunctival mullerectomy plus levator resection vs frontalis sling procedure 被引量:1
5
作者 Abolfazl Kasaee Mostafa Aliabadi +1 位作者 Laily Najafi Mansooreh Jamshidian-Tehrani 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2022年第8期1254-1260,共7页
AIM:To compare frontalis sling and tarsoconjunctival mullerectomy plus levator resection(TCMLR)in subjects with severe unilateral congenital ptosis with poor levator function(LF).METHODS:A prospective non-randomized n... AIM:To compare frontalis sling and tarsoconjunctival mullerectomy plus levator resection(TCMLR)in subjects with severe unilateral congenital ptosis with poor levator function(LF).METHODS:A prospective non-randomized nonblinded single center clinical trial.Fifty patients with severe unilateral congenital ptosis with poor LF were recruited.The frontalis sling and TCMLR were performed and the functional,cosmetic outcomes,complications,and success rate were evaluated at 1,3,and 6mo postoperatively.The t-test,Chi-square,Fishers exact,and nonparametric MannWhitney tests were used by SPSS software.RESULTS:Frontalis sling and TCMLR procedures were performed on 26 and 24 patients respectively.The mean age was 10.97±10.67y.LF was significantly better in the TCMLR group at months 1,3,and 6(P=0.002).Lagophthalmos was more common in the TCMLR group(no significant difference).At month 3,mild punctate epithelial erosions were observed more in the frontalis sling group(P=0.002).Significant complete success rate of 1st and 6th month for the frontalis sling vs TCMLR groups were 50%vs 20.8%(P=0.02),and 38.4%vs 50%(P=0.03)respectively.CONCLUSION:Complete success rate of TCMLR is higher in long-term follow-up in contrast with the frontalis sling in the short-term.Transient complications are more detected in mid-term follow-ups in both groups. 展开更多
关键词 tarsoconjunctival mullerectomy levator resection frontalis sling congenital ptosis
下载PDF
New autologous material for the frontalis suspension technique:superficial temporal fascia
6
作者 Elif Sari Hulda Rifat Ozakpinar Ali Teoman Tellioglu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2016年第2期315-317,共3页
The frontalis suspension technique, which is a well-known surgical procedure, has been used for congenital ptosis for many years [1]. Several autologous and foreign grafts or materials have been preferred for this sus... The frontalis suspension technique, which is a well-known surgical procedure, has been used for congenital ptosis for many years [1]. Several autologous and foreign grafts or materials have been preferred for this suspension, such as collagen, tensor fascia lata, palmaris longus tendon, deep temporal fascia, catgut, prolene, silicone, stainless steel, and supramid suture . Each of these have their own advantages and disadvantages; however, the superficial temporal fascia has not been used for the frontalis suspension method before. 展开更多
关键词 FIGURE New autologous material for the frontalis suspension technique
下载PDF
Comparison of the ability of wavelet index and bispectral index for reflecting regain of consciousness in patients undergone surgery 被引量:13
7
作者 ZHANG Xiao-tong CHENG Hao XIONG Wei WANG Bao-guo 《Chinese Medical Journal》 SCIE CAS CSCD 2010年第12期1520-1523,共4页
Background Wavelet index (WLI) is a new parameter for monitoring depth of anesthesia based on Wavelet analysis. We observed the change of WLI and bispectral index (BIS) in patients regain of consciousness (ROC) ... Background Wavelet index (WLI) is a new parameter for monitoring depth of anesthesia based on Wavelet analysis. We observed the change of WLI and bispectral index (BIS) in patients regain of consciousness (ROC) in the absence of frontalis electromyographic (EMG) interference. The aim of this study was to compare the ability of WLI and BIS for reflecting ROC in patients undergone surgery. Methods Twelve patients who were scheduled for maxillofacial surgeries were enrolled in the study. BIS and WLI values were monitored continually. After being given midazolam and remifentanil, patients were asked to squeeze the investigator's hand ever 30 seconds. Patients were continuously given propofol until loss of consciousness (LOC1). Tunstalrs isolated forearm technique was used to test the surgical consciousness in patients. After total muscle relaxation endotracheal intubations were performed, and the patients were connected to a ventilator. Then, propofol was withdrawn until the patients showed regain of consciousness (ROC1) and an awareness reaction. After the command test, patients were readministered with propofol until loss of consciousness (LOC2). After surgery, all of the sedatives were withdrawn, and the patients were let to regain consciousness (ROC2), Results The BIS values of twelve patients at ROC1 after using muscle relaxant were much lower than those at LOC1 and ROC2 without using muscle relaxant, showing statistical significance (P 〈0.05). Meanwhile, the WLI values of twelve patients at ROC1 after using muscle relaxant were much higher than those at LOC1 and equal to ROC2 without using muscle relaxant, showing statistical significances (P 〈0.05) between ROC1 and LOCI. Conclusions This study showed that under muscle relaxation or facial paralysis, when there is no EMG signal, BIS can not accurately reflect regain of consciousness in surgical patients, but WLI can reflect it accurately. So WLI may have advantages for reflecting state of consciousness in surgical patients. 展开更多
关键词 frontalis electromyographic activity wavelet index bispectral index tunstall's isolated forearmexperiments regain of consciousness
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部