AIM To determine whether fructo-oligosaccharide(FOS) affects visceral sensitivity, inflammation, and production of intestinal short-chain fatty acids(SCFA) in an irritable bowel syndrome(IBS) mouse model.METHODS Mice ...AIM To determine whether fructo-oligosaccharide(FOS) affects visceral sensitivity, inflammation, and production of intestinal short-chain fatty acids(SCFA) in an irritable bowel syndrome(IBS) mouse model.METHODS Mice were randomly assigned to daily oral gavage of saline solution with or without FOS(8 g/kg body weight) for 14 d. Mice were further assigned to receive either daily one-hour water avoidance stress(WAS) or sham-WAS for the first 10 d. After 2 wk, visceral sensitivity was measured by abdominal withdrawal reflex in response to colorectal distension and mucosal inflammation was evaluated. Gas chromatography, real-time reverse transcription PCR, and immunohistochemistry assays were used to quantify cecal concentrations of SCFA, intestinal cytokine expression, and number of intestinal mast cells per high-power field(HPF), respectively.RESULTS Mice subjected to WAS exhibited visceral hypersensitivity and low-grade inflammation. Among mice subjected to WAS, FOS increased visceral hypersensitivity and led to higher cecal concentrations of acetic acid(2.49 ± 0.63 mmol/L vs 1.49 ± 0.72 mmol/L, P < 0.05), propionic acid(0.48 ± 0.09 mmol/L vs 0.36 ± 0.05 mmol/L, P < 0.01), butyric acid(0.28 ± 0.09 mmol/L vs 0.19 ± 0.003 mmol/L, P < 0.05), as well as total SCFA(3.62 ± 0.87 mmol/L vs 2.27 ± 0.75 mmol/L, P < 0.01) compared to saline administration. FOS also increased ileal interleukin(IL)-23 mR NA(4.71 ± 4.16 vs 1.00 ± 0.99, P < 0.05) and colonic IL-1β mR NA(2.15 ± 1.68 vs 0.88 ± 0.53, P < 0.05) expressions as well as increased mean mast cell counts in the ileum(12.3 ± 2.6 per HPF vs 8.3 ± 3.6 per HPF, P < 0.05) and colon(6.3 ± 3.2 per HPF vs 3.4 ± 1.2 per HPF, P < 0.05) compared to saline administration in mice subjected to WAS. No difference in visceral sensitivity, intestinal inflammation, or cecal SCFA levels was detected with or without FOS administration in mice subjected to sham-WAS.CONCLUSION FOS administration intensifies visceral hypersensitivity and gut inflammation in stress-induced IBS mice, but not in the control mice, and is also associated with increased intestinal SCFA production.展开更多
Properties of enzyme production of a Bacillus subtitles strain （ MSJ-5 ） isolated from soil were studied in the test. Enzyme production reached the peak when MSJ-5 being cultured in fermentation medium for 32 h. 6-1...Properties of enzyme production of a Bacillus subtitles strain （ MSJ-5 ） isolated from soil were studied in the test. Enzyme production reached the peak when MSJ-5 being cultured in fermentation medium for 32 h. 6-1,4-D-mannanase was the main component of crude enzyme solution, test results showed that the optimal reaction temperature of the enzyme was 50 ℃, the optimum reaction pH was 7.0, and the enzyme was stable when pH was 5.0 -7.0. Hydrolysis test re- suits showed that β-1,4-D-mannanase produced by MSJ-5 had significant viscosity reduction effects on Konjak mannan, and the major hydrolysis product was man- nan oligosaecharide. The results indicated that 6-1,4-D-mannanase produced by B. subtilis MSJ-5 had application potential in feed ingredients and functional oligo- saccharides industry.展开更多
目的探讨低聚果糖的降血脂作用和机制。方法体内实验:30只昆明种小鼠随机分为对照组、实验低剂量、高剂量共3组,每组10只。实验组小鼠分别灌胃低聚果糖0.8,2.5 g/kg,对照组灌胃等剂量蒸馏水,一日1次,连续30 d,测定血清和肝组织中甘油三...目的探讨低聚果糖的降血脂作用和机制。方法体内实验:30只昆明种小鼠随机分为对照组、实验低剂量、高剂量共3组,每组10只。实验组小鼠分别灌胃低聚果糖0.8,2.5 g/kg,对照组灌胃等剂量蒸馏水,一日1次,连续30 d,测定血清和肝组织中甘油三酯(TG)、总胆固醇(TC)含量。体外实验:测定低聚果糖对高脂血症模型动物血清胆固醇的吸附能力;模拟人体胃和肠道p H环境,取一定量低聚果糖,分别加入猪油、花生油、蛋黄,测定不同p H时低聚果糖吸附甘油三酯和胆固醇的能力。结果体内实验中,实验组小鼠血清TG含量低于对照组(P<0.01)。低聚果糖对高脂血症模型动物血清胆固醇的吸附率为46.87%。p H 3.0,p H 7.0时,低聚果糖对猪油的吸附能力较强;p H 7.0时,对花生油和胆固醇的吸附能力较强。结论低聚果糖能降低正常小鼠血清TG含量,并可减少胃肠道对猪油、花生油和胆固醇的吸收,提示低聚果糖是影响脂类吸收、代谢的重要因素。展开更多
文摘AIM To determine whether fructo-oligosaccharide(FOS) affects visceral sensitivity, inflammation, and production of intestinal short-chain fatty acids(SCFA) in an irritable bowel syndrome(IBS) mouse model.METHODS Mice were randomly assigned to daily oral gavage of saline solution with or without FOS(8 g/kg body weight) for 14 d. Mice were further assigned to receive either daily one-hour water avoidance stress(WAS) or sham-WAS for the first 10 d. After 2 wk, visceral sensitivity was measured by abdominal withdrawal reflex in response to colorectal distension and mucosal inflammation was evaluated. Gas chromatography, real-time reverse transcription PCR, and immunohistochemistry assays were used to quantify cecal concentrations of SCFA, intestinal cytokine expression, and number of intestinal mast cells per high-power field(HPF), respectively.RESULTS Mice subjected to WAS exhibited visceral hypersensitivity and low-grade inflammation. Among mice subjected to WAS, FOS increased visceral hypersensitivity and led to higher cecal concentrations of acetic acid(2.49 ± 0.63 mmol/L vs 1.49 ± 0.72 mmol/L, P < 0.05), propionic acid(0.48 ± 0.09 mmol/L vs 0.36 ± 0.05 mmol/L, P < 0.01), butyric acid(0.28 ± 0.09 mmol/L vs 0.19 ± 0.003 mmol/L, P < 0.05), as well as total SCFA(3.62 ± 0.87 mmol/L vs 2.27 ± 0.75 mmol/L, P < 0.01) compared to saline administration. FOS also increased ileal interleukin(IL)-23 mR NA(4.71 ± 4.16 vs 1.00 ± 0.99, P < 0.05) and colonic IL-1β mR NA(2.15 ± 1.68 vs 0.88 ± 0.53, P < 0.05) expressions as well as increased mean mast cell counts in the ileum(12.3 ± 2.6 per HPF vs 8.3 ± 3.6 per HPF, P < 0.05) and colon(6.3 ± 3.2 per HPF vs 3.4 ± 1.2 per HPF, P < 0.05) compared to saline administration in mice subjected to WAS. No difference in visceral sensitivity, intestinal inflammation, or cecal SCFA levels was detected with or without FOS administration in mice subjected to sham-WAS.CONCLUSION FOS administration intensifies visceral hypersensitivity and gut inflammation in stress-induced IBS mice, but not in the control mice, and is also associated with increased intestinal SCFA production.
基金Supported by National Public Welfare Industry(Agriculture)Scientific Special Project(201203001)Shandong Agricultural Sciences Youth Fund Project(2014QNM21)Shandong Independent Innovation Achievements Special(2013ZHZX2A0402)
文摘Properties of enzyme production of a Bacillus subtitles strain （ MSJ-5 ） isolated from soil were studied in the test. Enzyme production reached the peak when MSJ-5 being cultured in fermentation medium for 32 h. 6-1,4-D-mannanase was the main component of crude enzyme solution, test results showed that the optimal reaction temperature of the enzyme was 50 ℃, the optimum reaction pH was 7.0, and the enzyme was stable when pH was 5.0 -7.0. Hydrolysis test re- suits showed that β-1,4-D-mannanase produced by MSJ-5 had significant viscosity reduction effects on Konjak mannan, and the major hydrolysis product was man- nan oligosaecharide. The results indicated that 6-1,4-D-mannanase produced by B. subtilis MSJ-5 had application potential in feed ingredients and functional oligo- saccharides industry.
文摘目的探讨低聚果糖的降血脂作用和机制。方法体内实验:30只昆明种小鼠随机分为对照组、实验低剂量、高剂量共3组,每组10只。实验组小鼠分别灌胃低聚果糖0.8,2.5 g/kg,对照组灌胃等剂量蒸馏水,一日1次,连续30 d,测定血清和肝组织中甘油三酯(TG)、总胆固醇(TC)含量。体外实验:测定低聚果糖对高脂血症模型动物血清胆固醇的吸附能力;模拟人体胃和肠道p H环境,取一定量低聚果糖,分别加入猪油、花生油、蛋黄,测定不同p H时低聚果糖吸附甘油三酯和胆固醇的能力。结果体内实验中,实验组小鼠血清TG含量低于对照组(P<0.01)。低聚果糖对高脂血症模型动物血清胆固醇的吸附率为46.87%。p H 3.0,p H 7.0时,低聚果糖对猪油的吸附能力较强;p H 7.0时,对花生油和胆固醇的吸附能力较强。结论低聚果糖能降低正常小鼠血清TG含量,并可减少胃肠道对猪油、花生油和胆固醇的吸收,提示低聚果糖是影响脂类吸收、代谢的重要因素。
