Quality and yield are the primary concerns in kiwifruit breeding,but research on the genetic mechanisms of fruit size,shape,and ascorbic acid(ASA)content is currently very limited,which restricts the development of ki...Quality and yield are the primary concerns in kiwifruit breeding,but research on the genetic mechanisms of fruit size,shape,and ascorbic acid(ASA)content is currently very limited,which restricts the development of kiwifruit molecular breeding.In this study,we obtained a total of 8.88 million highly reliable single nucleotide polymorphism(SNP)markers from 140 individuals from the natural hybrid offspring of Actinidia eriantha cv.‘White’using whole genome resequencing technology.A genome-wide association study was conducted on eight key agronomic traits,including single fruit weight,fruit shape,ASA content,and the number of inflorescences per branch.A total of 59 genetic loci containing potential functional genes were located,and candidate genes related to single fruit weight,fruit length,ASA content,number of inflorescences per branch and other traits were identified within the candidate interval,such as AeWUSCHEL,AeCDK1(cell cycle dependent kinase),AeAO1(ascorbic oxidase)and AeCO1(CONSTANS-like 4).After constructing an RNAi vector for AeAO1 and injecting it into the fruit of cv.‘Midao 31’to interfere with the expression of the AeAO1 gene,the results showed that the activity of ascorbic oxidase in the fruit of‘Midao 31’significantly decreased,while the content of ASA significantly increased.This study provides valuable insights into the genetic basis of variation in A.eriantha fruit traits,which may benefit molecular marker-assisted breeding efforts.展开更多
Establishing the Greater Food Approach and promoting the Yangtze River Economic Belt s national major regional development strategy can better support and serve the agricultural power and Chinese-style modernization.T...Establishing the Greater Food Approach and promoting the Yangtze River Economic Belt s national major regional development strategy can better support and serve the agricultural power and Chinese-style modernization.This paper introduces the characteristics of fruit industry in 16 autonomous prefectures and 47 autonomous counties under the jurisdiction of the Yangtze River Economic Belt.It studies the intellectual property resources of brand marks from the aspects of geographical indications,collective trademarks,certification trademarks,well-known trademarks in China and national design patents,and analyzes the main problems of brand and high-quality development of fruit industry in these ethnic autonomous areas.Finally,it puts forward some strategies,such as improving the protection of intellectual property rights of geographical indications,using intellectual property rights of brand signs,building modern seed industry upgrading project,drawing lessons from the experience of thousand villages demonstration project,ensuring that large-scale poverty does not occur,and building a diversified food supply system.展开更多
The baobab, Adansonia digitata L., plays an important role in the economy of local populations. Nowadays, baobab seed oil is highly prized for its many cosmetic and therapeutic applications, and for its composition of...The baobab, Adansonia digitata L., plays an important role in the economy of local populations. Nowadays, baobab seed oil is highly prized for its many cosmetic and therapeutic applications, and for its composition of unsaturated fatty acids, sterols, and tocopherols. However, it undergoes numerous reactions during production, processing, transport, and storage, leading to undesirable products that make it unstable. The aim of this study was to provide local processors with innovative solutions for the treatment of unrefined vegetable oils. To this end, an experimental device for filtering crude oil on activated carbon made from fruit capsules was designed. The results obtained after the treatment show a significant decrease at (p < 5%) in acid value (1.62 to 0.58 mg KOH/g), peroxide value (4.40a to 0.50c mEqO<sub>2</sub>/Kg), chlorophyll concentration (1.81 to 0.50 mg/Kg) and primary and secondary oxidation products. According to these results, activated carbon’s adsorptive power eliminates oxidation products and certain pro-oxidants such as chlorophyll, resulting in a cleaner, more stable and better storable oil.展开更多
To study the effect of exogenous selenium on fruit quality in walnut(Juglans regia L.),8-year-old walnut(Qingxiang)was taken as the research object.In the fruit expansion stage,300 mg/L of sodium selenate,yeast seleni...To study the effect of exogenous selenium on fruit quality in walnut(Juglans regia L.),8-year-old walnut(Qingxiang)was taken as the research object.In the fruit expansion stage,300 mg/L of sodium selenate,yeast selenium and sodium selenite solutions were applied on the leaf of walnut,and the selenium levels in leaves,pericarp and kernel were determined at the ripening stage.The fruit quality,mineral nutrient content,antioxidant enzyme activity,and related genes’expression were analyzed.The results showed that the three exogenous selenium increased the selenium levels in leaves,pericarp and kernel of walnut.They also significantly increased fruit and kernel weights,and kernel linoleic acid,but markedly decreased kernel crude fat and saturated fatty acid.Selenium spraying promoted the absorption of mineral nutrients such as potassium and zinc,but inhibited the absorption of calcium,and had no significant effect on iron and magnesium in the kernel.Three exogenous selenium increased the activities of superoxide dismutase(SOD),peroxidase(POD),ascorbate peroxidase(APX),and catalase(CAT)significantly in the kernel.Except for sodium selenate treatment significantly reduced malondialdehyde(MDA)content in the kernel,the other two selenium sources treatments had no significant effect on MDA and hydrogen peroxide(H2O2)levels.They also increased the expression of JrCu/Zn-SOD,Jr2-Cys POD,JrCyt-APX and JrCAT in kernel,to different extents.These implies that,in the walnut fruit enlargement period,the foliar spraying selenium could increase the selenium content of walnut,affect the mineral nutrient absorption,improve the antioxidant capacity and related genes’expression,and reduce the degree of peroxide,and then improve the quality of fruit.Furthermore,yeast selenium showed the comprehensive effect of the best.展开更多
The effect of soil nutrient content on fruit yield and fruit quality is very important.To explore the effect of soil nutrients on apple quality we investigated 200 fruit samples from 40 orchards in Feng County,Jiangsu...The effect of soil nutrient content on fruit yield and fruit quality is very important.To explore the effect of soil nutrients on apple quality we investigated 200 fruit samples from 40 orchards in Feng County,Jiangsu Province.Soil mineral elements and fruit quality were measured.The effect of soil nutrient content on fruit quality was analyzed by artificial neural network(ANN)model.The results showed that the prediction accuracy was highest(R2=0.851,0.847,0.885,0.678 and 0.746)in mass per fruit(MPF),hardness(HB),soluble solids concentrations(SSC),titratable acid concentration(TA)and solid-acid ratio(SSC/TA),respectively.The sensitivity analysis of the prediction model showed that soil available P,K,Ca and Mg contents had the greatest impact on the quality of apple fruit.Response surface method(RSM)was performed to determine the optimum range of the available P,K,Ca,and Mg contents in orchards In Feng County,which were 10∼20 mg⋅kg^(−1),170∼200 mg⋅kg^(−1),1000∼1500 mg⋅kg^(−1),and 80∼200 mg⋅kg^(−1),respectively.The research also concluded that improving the content of available P and available Ca in orchard soil was crucial to improve apple fruit quality in Feng County,Jiangsu Province.展开更多
Sugar plays an important role in apple fruit development,appearance and quality as well as contributing to a plant’s water stress response.Trehalose and the trehalose biosynthetic metabolic pathways are part of the s...Sugar plays an important role in apple fruit development,appearance and quality as well as contributing to a plant’s water stress response.Trehalose and the trehalose biosynthetic metabolic pathways are part of the sugar signaling system in plants,which are important regulator of water stress response in apple.The effect of water stress treatments applied to apple trees and the corresponding effects of ABA on developmental fruit quality were examined for indicators of fruit quality during fruit development.The results indicated that the severe water stress treatment(W2)occurring after the last stage of fruit cell division caused a decrease in the color and size of fruit.The moderate water stress(W1)occurring after the last stage of fruit cell enlargement(S2)caused an increase in the content of fructose and sorbitol while the apple fruit shape was not affected.These changes in sugar are related to the activity of sugar metabolic enzymes.While the enzymatic activity of vacuolar acid invertase(vAINV)was higher,that of sucrose-phosphate synthase(SPS)was lower in water stress treated fruit throughout the developmental period.This indicates that enhanced sucrose degradation and reduced sucrose synthesis leads to an overall reduced sucrose content during times of drought.Thus,water stress reduced sucrose content.Whereas the content of endogenous trehalose and ABA were the highest in water stress treated fruit.A moderate water stress(W1)imposed on apple trees via water restriction(60%–65%of field capacity)after the fruit cell enlargement phase of fruit development yielded sweeter fruit of higher economic value.展开更多
For a long time,seasonal drought occurs frequently in Southwest China,and the management of water and fertilizer in kiwifruit orchards has no quantitative standards,which seriously affects the yield and quality of kiw...For a long time,seasonal drought occurs frequently in Southwest China,and the management of water and fertilizer in kiwifruit orchards has no quantitative standards,which seriously affects the yield and quality of kiwifruit.Therefore,the effects of water and fertilizer deficit regulation with drip irrigation(WFDRDI)on the quality of kiwifruit at different growth stages were explored to achieve water and fertilizer saving,and green and efficient production of kiwifruit.We select‘Jin Yan'kiwifruit and set two water deficit levels(W_(D20%)and W_(D40%))and three fertilizer deficit levels(F_(D15%),F_(D30%)and F_(D45%))at bud burst to leafing stage(stageⅠ),flowering to fruit set stage(stageⅡ),fruit expansion stage(stageⅢ)and fruit maturation stage(stageⅣ),respectively,with a full irrigation and fertilization as the control treatment(CK)in 2017and 2018.Results showed that the WFDRDI at stageⅡandⅢhad significant effect on fruit physical quality of kiwifruit,specifically,theⅢ-WD40%F_(D30%)andⅢ-W_(D20%)F_(D45%)treatments significantly increased fruit firmness by 13.62 and 15.59%(P<0.05),respectively;theⅡ-W_(D40%)F_(D15%)andⅢ-W_(D40%)F_(D15%)treatments significantly increased dry matter by 8.19 and 6.47%(P<0.05),respectively;theⅢ-W_(D20%)F_(D15%)treatment significantly increased single fruit weight and fruit volume by 9.33 and 12.65%(P<0.05),respectively;theⅡ-W_(D20%)F_(D15%)treatment significantly increased fruit water content by 1.99%(P<0.05).The WFDRDI had an obvious effect on fruit chemical quality of kiwifruit.TheⅢ-W_(D20%)F_(D45%),Ⅳ-W_(D40%)F_(D15%)andⅣ-W_(D20%)F_(D30%)treatments significantly increased vitamin C(Vc)content by 69.96,36.96 and 34.31%(P<0.05),respectively;theⅢ-W_(D40%)F_(D15%)andⅣ-W_(D40%)F_(D15%)treatments significantly increased total soluble solid(TSS)content by 3.79 and 17.05%(P<0.05),respectively,and significantly increased soluble sugar content by 28.61 and 34.79%(P<0.05),respectively;the contents of fructose,glucose and sucrose also had a significantly increasing trend,which was increased significantly by 5.58–19.63%,40.55–60.36%and 54.03–54.92%in theⅢ-WD40%F_(D15%)andⅣ-W_(D40%)F_(D15%)treatments(P<0.05),respectively;sugar–acid ratio was increased significantly in theⅣ-W_(D40%)F_(D15%)treatment by 64.65%(P<0.05).The degree and duration of water and fertilizer deficit had a comprehensive effect on fruit quality of kiwifruit.The WFDRDI at stageⅡandⅢcontribute to improving fruit physical quality,and the threshold of water and fertilizer deficit were 20 and 15%,respectively;stageⅢandⅣare the critical periods for improving fruit chemical quality by water and fertilizer coupling effect,and the threshold of water and fertilizer deficit were 40 and 15%,respectively.Therefore,aiming at precise water and fertilizer saving,theⅠ-W_(D20%)F_(D30%),Ⅱ-W_(D40%)F_(D15%),Ⅲ-W_(D40%)F_(D15%)andⅣ-W_(D40%)F_(D15%)treatments under WFDRDI during the whole growth period of kiwifruit were the best mode to improve quality and production of kiwifruit.展开更多
Olive productivity should be improved through stimulating nutrition,particularly under poor fertility soils.Consequently,the objective of this study was to assess the efficacy of applying organic and bio-fertilizers o...Olive productivity should be improved through stimulating nutrition,particularly under poor fertility soils.Consequently,the objective of this study was to assess the efficacy of applying organic and bio-fertilizers on the physiological growth,yield and fruit quality of olive trees under newly reclaimed poor-fertility sandy soil in an arid environment.During a field experiment carried out at El-Qantara,North Sinai,Egypt over two consecutive seasons(2019-2020 and 2020-2021),olive Kalamata trees were evaluated under three organic fertilizer treatments alone or in combination with three bio-fertilizers treatments.Organic fertilizer was applied as goat manure(16.8 kg/tree/year),or olive pomace(8.5 kg/tree/year)in mid-December of each season vs.untreated trees.The bio-fertilizers were applied as N-fixing bacteria(150 g/tree)was inculated in early March of each season,or amino acid mixture(1.5%)was applied three times,at 70%of full bloom,21 days after full bloom,and a month later in comparison to a non-fertilized trees(control).The cultivar used was Kalamata,a dual-purpose cultivar for oil and table olives whose value increases when processed as table olives.The results indicated that the goat manure followed by olive pomace significantly enhanced photosynthetic pigments(chlorophyll a,b,and carotenoids),leaf mineral contents(N,P,K,Ca,Mg and Fe),tree canopy volume,number of flowers per inflorescence,number of inflorescences per shoot,initial fruit set,fruit retention.For fruit quality,fruit length and width,fruit weight,and total fruit yield was increased compared to the non-fertilized control.Likewise,The bio-fertilizer N-fixing bacteria followed by the amino acid mixture significantly improved all of the aforementioned parameters.Accordingly,it is recommended,both environmentally and economically to utilize organic and bio-fertizers,particularly goat manure combined with N-fixing bacteria,in low-fertility soil to sustain olive production as well as reducing mineral fertilization.展开更多
The flesh color of pummelo(Citrus maxima)fruits is highly diverse and largely depends on the level of carotenoids,which are beneficial to human health.It is vital to investigate the regulatory network of carotenoid bi...The flesh color of pummelo(Citrus maxima)fruits is highly diverse and largely depends on the level of carotenoids,which are beneficial to human health.It is vital to investigate the regulatory network of carotenoid biosynthesis to improve the carotenoid content in pummelo.However,the molecular mechanism underlying carotenoid accumulation in pummelo is not fully understood.In this study,we identified a novel histone methyltransferase gene,CgSDG40,involved in carotenoid regulation by analyzing the flesh transcriptome of typical white-fleshed pummelo,red-fleshed pummelo and extreme-colored F1 hybrids from a segregated pummelo population.Expression of CgSDG40 corresponded to flesh color change and was highly coexpressed with CgPSY1.Interestingly,CgSDG40 and CgPSY1 are located physically adjacent to each other on the chromosome in opposite directions,sharing a partially overlapping promoter region.Subcellular localization analysis indicated that CgSDG40 localizes to the nucleus.Overexpression of CgSDG40 significantly increased the total carotenoid content in citrus calli relative to that in wild type.In addition,expression of CgPSY1 was significantly activated in overexpression lines relative to wild type.Taken together,our findings reveal a novel histone methyltransferase regulator,CgSDG40,involved in the regulation of carotenoid biosynthesis in citrus and provide new strategies for molecular design breeding and genetic improvement of fruit color and nutritional quality.展开更多
Habanero pepper(Capsicum chinense Jacq.)is a crop of economic relevance in the Peninsula of Yucatan.Its fruits have a high level of capsaicinoids compared to peppers grown in other regions of the world,which gives the...Habanero pepper(Capsicum chinense Jacq.)is a crop of economic relevance in the Peninsula of Yucatan.Its fruits have a high level of capsaicinoids compared to peppers grown in other regions of the world,which gives them industrial importance.Soil is an important factor that affects pepper development,nutritional quality,and capsaicinoid content.However,the effect of soil type on fruit development and capsaicinoid metabolism has been little understood.This work aimed to compare the effect of soils with contrasting characteristics,black soil(BS)and red soil(RS),on the expression of genes related to the development of fruits,and capsaicinoid synthesis using a transcriptomic analysis of the habanero pepper fruits.Plants growing in RS had bigger fruits and higher expression of genes related to floral development,fruit abscission,and softening which suggests that RS stimulates fruit development from early stages until maturation stages.Fruits from plants growing in BS had enrichment in metabolic pathways related to growth,sugars,and photosynthesis.Besides,these fruits had higher capsaicinoid accumulation at 25 days post-anthesis,and higher expression of genes related to the branched-chain amino acids metabolism(ketol-acid reductisomerase KARI),pentose phosphate pathway and production of NADPH(glucose-6-phosphate-1-dehydrogenase G6PDH),and proteasome and vesicular traffic in cells(26S proteasome regulatory subunit T4 RPT4),which suggest that BS is better in the early stimulation of pathways related to the nutritional quality and capsaicinoid metabolism in the fruits.展开更多
Increasing photosynthesis and light capture offers possibilities for improving crop yield and provides a sustainable way to meet the increasing global demand for food.However,the poor light transmittance of transparen...Increasing photosynthesis and light capture offers possibilities for improving crop yield and provides a sustainable way to meet the increasing global demand for food.However,the poor light transmittance of transparent plastic films and shade avoidance at high planting density seriously reduce photosynthesis and alter fruit quality in vegetable crops,and therefore it is important to investigate the mechanisms of light signaling regulation of photosynthesis and metabolism in tomato(Solanum lycopersicum).Here,a combination of red,blue,and white(R1W1B0.5)light promoted the accumulation of chlorophyll,carotenoid,and anthocyanin,and enhanced photosynthesis and electron transport rates by increasing the density of active reaction centers and the expression of the genes LIGHT-HARVESTING COMPLEX B(SlLHCB)and A(SlLHCA),resulting in increased plant biomass.In addition,R1W1B0.5 light induced carotenoid accumulation and fruit ripening by decreasing the expression of LYCOPENEβ-CYCLASE(SlCYCB).Disruption of SlCYCB largely induced fruit lycopene accumulation,and reduced chlorophyll content and photosynthesis in leaves under red,blue,and white light.Molecular studies showed that ELONGATED HYPOCOTYL 5(SlHY5)directly activated SlCYCB,SlLHCB,and SlLHCA expression to enhance chlorophyll accumulation and photosynthesis.Furthermore,R1W1B0.5 light-induced chlorophyll accumulation,photosynthesis,and SlHY5 expression were largely decreased in the slphyb1cry1 mutant.Collectively,R1W1B0.5 light noticeably promoted photosynthesis,biomass,and fruit quality through the photoreceptor(SlPHYB1 and SlCRY1)-SlHY5-SlLHCA/B/SlCYCB module in tomato.Thus,the manipulation of light environments in protected agriculture is a crucial tool to regulate the two vital agronomic traits related to crop production efficiency and fruit nutritional quality in tomato.展开更多
Background Providing high-quality roughage is crucial for improvement of ruminant production because it is an essential component of their feed.Our previous study showed that feeding bio-fermented rice straw(BF)improv...Background Providing high-quality roughage is crucial for improvement of ruminant production because it is an essential component of their feed.Our previous study showed that feeding bio-fermented rice straw(BF)improved the feed intake and weight gain of sheep.However,it remains unclear why feeding BF to sheep increased their feed intake and weight gain.Therefore,the purposes of this research were to investigate how the rumen micro-biota and serum metabolome are dynamically changing after feeding BF,as well as how their changes influence the feed intake,digestibility,nutrient transport,meat quality and growth performances of sheep.Twelve growing Hu sheep were allocated into 3 groups:alfalfa hay fed group(AH:positive control),rice straw fed group(RS:negative control)and BF fed group(BF:treatment).Samples of rumen content,blood,rumen epithelium,muscle,feed offered and refusals were collected for the subsequent analysis.Results Feeding BF changed the microbial community and rumen fermentation,particularly increasing(P<0.05)relative abundance of Prevotella and propionate production,and decreasing(P<0.05)enteric methane yield.The histomorphology(height,width,area and thickness)of rumen papillae and gene expression for carbohydrate trans-port(MCT1),tight junction(claudin-1,claudin-4),and cell proliferation(CDK4,Cyclin A2,Cyclin E1)were improved(P<0.05)in sheep fed BF.Additionally,serum metabolome was also dynamically changed,which led to up-regulating(P<0.05)the primary bile acid biosynthesis and biosynthesis of unsaturated fatty acid in sheep fed BF.As a result,the higher(P<0.05)feed intake,digestibility,growth rate,feed efficiency,meat quality and mono-unsaturated fatty acid concentration in muscle,and the lower(P<0.05)feed cost per kg of live weight were achieved by feeding BF.Conclusions Feeding BF improved the growth performances and meat quality of sheep and reduced their feed cost.Therefore,bio-fermentation of rice straw could be an innovative way for improving ruminant production with mini-mizing production costs.展开更多
[Objectives] This study conducted investigations on the climate and soil nutrients of different chestnut orchards in the Yanshan region, selected key ecological factors affecting the growth and fruit quality of chestn...[Objectives] This study conducted investigations on the climate and soil nutrients of different chestnut orchards in the Yanshan region, selected key ecological factors affecting the growth and fruit quality of chestnuts, and provided a theoretical basis for the cultivation, introduction, and scientific planting of high-quality chestnuts. [Methods] The ‘Yanshanzaofeng’ chestnuts in four orchards located in Qianxi, Qianan, Funing, and Qinglong of Hebei Science and Technology Normal University were selected. The climate and soil nutrient conditions of the four orchards were investigated. Growth indicators such as branch length and diameter, nut weight, and internal quality indicators such as starch, fat, and protein content were analyzed. Principal component analysis was conducted on nine climate factors and twelve soil factors in the four chestnut orchards to identify the most closely related ecological factors influencing chestnut growth and quality. [Results] (i) Different orchards had varying climate conditions, with Qianan orchard having higher rainfall than the others, Funing orchard having the highest number of sunshine hours in the growing season, and Qinglong orchard experiencing the greatest temperature difference during the growing season. (ii) Significant differences were found in soil nutrient content among the orchards, with the coefficient of variation for organic matter and mineral elements ranging from 19.1% (S) to 80.3% (available phosphorus). (iii) The main ecological factor influencing chestnut growth was the photosynthetic factor, while fruit quality was influenced by a combination of climatic factors, photosynthetic factors, and nutrient factors. Key ecological factor indicators included: annual precipitation, annual sunshine hours, growing season precipitation, growing season sunshine hours, soil organic matter, available phosphorus, available potassium, alkali nitrogen, copper, zinc, available boron, and sulfur. Soluble solids were significantly positively correlated with growing season sunshine hours, and fat was significantly positively correlated with available potassium and alkali nitrogen, and significantly positively correlated with available phosphorus. [Conclusions] Orchards with high growing season precipitation, long sunshine hours, and high organic matter and nitrogen-phosphorus-potassium content in the soil are more conducive to promoting the growth of ‘Yanshanzaofeng’ chestnuts and improving fruit quality.展开更多
Over the past few decades,the Internet has rapidly diffused across China.The spread of the Internet has had a profound economic and social impact on Chinese rural areas.Existing research shows that Internet access sig...Over the past few decades,the Internet has rapidly diffused across China.The spread of the Internet has had a profound economic and social impact on Chinese rural areas.Existing research shows that Internet access significantly impacts agricultural production and improves smallholder farmers’income.Beyond these,the Internet can affect other dimensions of social welfare.However,research about the impact of Internet access on dietary quality in rural China remains scarce.This study utilizes multi-period panel data from Fixed Observation Point in rural China from 2009 to 2015 to estimate the impact of Internet access on dietary quality and food consumption of rural households and conducts a causal analysis.Regression models with time and household fixed effects allow robust estimation while reducing potential issues of unobserved heterogeneity.The estimates show that Internet access has significantly increased rural household dietary quality(measured by the Chinese Diet Balance Index).Further research finds that Internet access has increased the consumption of animal products,such as aquatic and dairy products.We also examine the underlying mechanisms.Internet access improves dietary quality and food consumption mainly through increasing household income and food expenditure.These results encourage the promotion of Internet access as a valuable tool for nutritional improvements,especially in rural areas.展开更多
Jujube fruits usually suffer from physiological disorders or infectious diseases during storage,leading to quality deterioration,softening,or rotting.Therefore,this study aims to investigate the effect of methionine s...Jujube fruits usually suffer from physiological disorders or infectious diseases during storage,leading to quality deterioration,softening,or rotting.Therefore,this study aims to investigate the effect of methionine soaking treatment on the postharvest jujube fruit decay rate and storage quality.Methionine treatment significantly reduced the decay rate of postharvest jujube fruit and effectively maintained fruit color and titratable acid.Methionine treatment effectively delayed the decrease of firmness,soluble solids,ascorbic acid,and lignin contents in jujube fruit.Methionine treatment reduced the content of alanine and phenylalanine,and increased the content of glycine in jujube fruit,but displayed no significant effect on total amino acid content.In addition,jujube fruits in the methionine treatment group had a higher total phenolic and flavonoid content and antioxidant capacity,both in free and bound forms.Compared with the control,methionine treatment also significantly increased the content of individual phenolic acid fractions(gallic acid,vanillic acid,and syringic acid)and flavonoid fractions(catechin,epicatechin,rutin)in free form as well as individual phenolic acid fractions in bound form(gallic acid and ferulic acid)in jujube fruits.Overall,this study suggested that the methionine treatment could be used as a prospective preservative to reduce the postharvest decay of jujube fruit and alleviate its nutritional quality deterioration during cold storage at 4°C.展开更多
The dramatic increase in intracranial pressure after subarachnoid hemorrhage leads to a decrease in cerebral perfusion pressure and a reduction in cerebral blood flow.Mitochondria are directly affected by direct facto...The dramatic increase in intracranial pressure after subarachnoid hemorrhage leads to a decrease in cerebral perfusion pressure and a reduction in cerebral blood flow.Mitochondria are directly affected by direct factors such as ischemia,hypoxia,excitotoxicity,and toxicity of free hemoglobin and its degradation products,which trigger mitochondrial dysfunction.Dysfunctional mitochondria release large amounts of reactive oxygen species,inflammatory mediators,and apoptotic proteins that activate apoptotic pathways,further damaging cells.In response to this array of damage,cells have adopted multiple mitochondrial quality control mechanisms through evolution,including mitochondrial protein quality control,mitochondrial dynamics,mitophagy,mitochondrial biogenesis,and intercellular mitochondrial transfer,to maintain mitochondrial homeostasis under pathological conditions.Specific interventions targeting mitochondrial quality control mechanisms have emerged as promising therapeutic strategies for subarachnoid hemorrhage.This review provides an overview of recent research advances in mitochondrial pathophysiological processes after subarachnoid hemorrhage,particularly mitochondrial quality control mechanisms.It also presents potential therapeutic strategies to target mitochondrial quality control in subarachnoid hemorrhage.展开更多
Edible mushroom sexual spores have been gaining more interest due to their bioactive components and functions.Spore discharge(SD)is an important factor affecting the quality of edible mushrooms.In this review,the bioa...Edible mushroom sexual spores have been gaining more interest due to their bioactive components and functions.Spore discharge(SD)is an important factor affecting the quality of edible mushrooms.In this review,the bioactive nutrients of sexual spores of edible mushrooms were summarized,the SD mechanism was described,and the relationship between postharvest SD and the quality of edible mushrooms was analyzed.Spores contain various bioactive nutrients that are benefi cial to the human body.Mature mushrooms can actively discharge spores in a process affected by light,relative humidity,and temperature.During storage,the physiological metabolism of spore-bearing gill tissue is vigorous,promoting the release of postharvest spores and changing the nutritional value of fruiting bodies.The flavor of the fruiting bodies also varied signifi cantly during SD.Edible mushroom sexual spores have the potential to become new raw materials for functional food and medical resources.Research on the effect of the mechanism of SD on the quality of edible mushrooms and the development of SD regulation technology may be a new trend in the quality control of edible mushrooms,which will promote the development of the edible mushroom industry.展开更多
[Objectives]The paper was to provide a reference for screening dwarfing rootstock suitable for main spur-type Fuji cultivars in central and southern Hebei Province.[Methods]With spur-type Fuji‘Tianhong 2’as the mate...[Objectives]The paper was to provide a reference for screening dwarfing rootstock suitable for main spur-type Fuji cultivars in central and southern Hebei Province.[Methods]With spur-type Fuji‘Tianhong 2’as the material,the vegetative growth,yield and fruit quality of 8 different rootstock-scion combinations were compared.[Results]‘Tianhong 2’/SH6 as self-rooted rootstock had large average single fruit weight(256.33 g),large number of fruits per plant(188.68),the highest yield[(3250.08±23.42)kg/667 m ^(2)]and the highest colored area(93.5%),and the soluble solid content reached the requirement of high quality fruit(15.78%).[Conclusions]In central and southern Hebei Province,‘Tianhong 2’grafted on SH6 self-rooted rootstock has moderate growth,high yield and good fruit quality,so it can be considered as the preferred rootstock-scion combination in the local area.展开更多
Recently,the fundamental problem with Hybrid Mobile Ad-hoc Net-works(H-MANETs)is tofind a suitable and secure way of balancing the load through Internet gateways.Moreover,the selection of the gateway and overload of th...Recently,the fundamental problem with Hybrid Mobile Ad-hoc Net-works(H-MANETs)is tofind a suitable and secure way of balancing the load through Internet gateways.Moreover,the selection of the gateway and overload of the network results in packet loss and Delay(DL).For optimal performance,it is important to load balance between different gateways.As a result,a stable load balancing procedure is implemented,which selects gateways based on Fuzzy Logic(FL)and increases the efficiency of the network.In this case,since gate-ways are selected based on the number of nodes,the Energy Consumption(EC)was high.This paper presents a novel Node Quality-based Clustering Algo-rithm(NQCA)based on Fuzzy-Genetic for Cluster Head and Gateway Selection(FGCHGS).This algorithm combines NQCA with the Improved Weighted Clus-tering Algorithm(IWCA).The NQCA algorithm divides the network into clusters based upon node priority,transmission range,and neighbourfidelity.In addition,the simulation results tend to evaluate the performance effectiveness of the FFFCHGS algorithm in terms of EC,packet loss rate(PLR),etc.展开更多
Chinese plum(Prunus salicina Lindl.)originates from China and makes a large contribution to the global production of plums.The P.salicina‘Wushancuili'has a green coloration and high fruit quality and is economica...Chinese plum(Prunus salicina Lindl.)originates from China and makes a large contribution to the global production of plums.The P.salicina‘Wushancuili'has a green coloration and high fruit quality and is economically important in eliminating poverty and protecting ecology in the Yangtze River Three Gorges Reservoir.However,rain-induced cracking(rain-cracking,literally skin cracking caused by rain)is a limitation to‘Wushancuili'fruit production and causes severe losses.This study reported a high-quality‘Wushancuili'genome assembly consisting of a 302.17-Mb sequence with eight pseudo-chromosomes and a contig N50 of 23.59 Mb through the combination of Illumina sequencing,Pacific Biosciences HiFiⅢsequencing,and high-throughput chromosome conformation capture technology.A total of 25109 protein-coding genes are predicted and 54.17%of the genome is composed of repetitive sequences.‘Wushancuili'underwent a remarkable orthoselection during evolution.Gene identification revealed that loss-of-function in four core MYB10 genes results in the anthocyanin deficiency and absence of red color,revealing the green coloration due to the residual high chlorophyll in fruit skin.Besides,the occurrence of cracking is assumed to be closely associated with cell wall modification and frequently rain-induced pathogen enrichment through transcriptomic analysis.The loss of MYB10 genes might render fruit more susceptible to pathogen-mediated cracking by weakening the epidermal strength and reactive oxygen species(ROS)scavenging.Our findings provided fundamental knowledge regarding fruit coloration and rain-cracking and will facilitate genetic improvement and cultivation management in Chinese plums.展开更多
基金supported by the National Natural Science Foundation of China(U23A20204,31972474 and 31471157).
文摘Quality and yield are the primary concerns in kiwifruit breeding,but research on the genetic mechanisms of fruit size,shape,and ascorbic acid(ASA)content is currently very limited,which restricts the development of kiwifruit molecular breeding.In this study,we obtained a total of 8.88 million highly reliable single nucleotide polymorphism(SNP)markers from 140 individuals from the natural hybrid offspring of Actinidia eriantha cv.‘White’using whole genome resequencing technology.A genome-wide association study was conducted on eight key agronomic traits,including single fruit weight,fruit shape,ASA content,and the number of inflorescences per branch.A total of 59 genetic loci containing potential functional genes were located,and candidate genes related to single fruit weight,fruit length,ASA content,number of inflorescences per branch and other traits were identified within the candidate interval,such as AeWUSCHEL,AeCDK1(cell cycle dependent kinase),AeAO1(ascorbic oxidase)and AeCO1(CONSTANS-like 4).After constructing an RNAi vector for AeAO1 and injecting it into the fruit of cv.‘Midao 31’to interfere with the expression of the AeAO1 gene,the results showed that the activity of ascorbic oxidase in the fruit of‘Midao 31’significantly decreased,while the content of ASA significantly increased.This study provides valuable insights into the genetic basis of variation in A.eriantha fruit traits,which may benefit molecular marker-assisted breeding efforts.
基金Supported by Special Soft Science Research Project for Hubei Province Science and Technology Innovation Talents and Services(2022EDA060).
文摘Establishing the Greater Food Approach and promoting the Yangtze River Economic Belt s national major regional development strategy can better support and serve the agricultural power and Chinese-style modernization.This paper introduces the characteristics of fruit industry in 16 autonomous prefectures and 47 autonomous counties under the jurisdiction of the Yangtze River Economic Belt.It studies the intellectual property resources of brand marks from the aspects of geographical indications,collective trademarks,certification trademarks,well-known trademarks in China and national design patents,and analyzes the main problems of brand and high-quality development of fruit industry in these ethnic autonomous areas.Finally,it puts forward some strategies,such as improving the protection of intellectual property rights of geographical indications,using intellectual property rights of brand signs,building modern seed industry upgrading project,drawing lessons from the experience of thousand villages demonstration project,ensuring that large-scale poverty does not occur,and building a diversified food supply system.
文摘The baobab, Adansonia digitata L., plays an important role in the economy of local populations. Nowadays, baobab seed oil is highly prized for its many cosmetic and therapeutic applications, and for its composition of unsaturated fatty acids, sterols, and tocopherols. However, it undergoes numerous reactions during production, processing, transport, and storage, leading to undesirable products that make it unstable. The aim of this study was to provide local processors with innovative solutions for the treatment of unrefined vegetable oils. To this end, an experimental device for filtering crude oil on activated carbon made from fruit capsules was designed. The results obtained after the treatment show a significant decrease at (p < 5%) in acid value (1.62 to 0.58 mg KOH/g), peroxide value (4.40a to 0.50c mEqO<sub>2</sub>/Kg), chlorophyll concentration (1.81 to 0.50 mg/Kg) and primary and secondary oxidation products. According to these results, activated carbon’s adsorptive power eliminates oxidation products and certain pro-oxidants such as chlorophyll, resulting in a cleaner, more stable and better storable oil.
基金supported by the Science and Technology Planning Project of Shannan Science and Technology Bureau of Tibet(2021Z21008)the Hubei Province‘14th Five-Year’Major Science and Technology Aid Tibet Project(SCXX-XZCG-22016).
文摘To study the effect of exogenous selenium on fruit quality in walnut(Juglans regia L.),8-year-old walnut(Qingxiang)was taken as the research object.In the fruit expansion stage,300 mg/L of sodium selenate,yeast selenium and sodium selenite solutions were applied on the leaf of walnut,and the selenium levels in leaves,pericarp and kernel were determined at the ripening stage.The fruit quality,mineral nutrient content,antioxidant enzyme activity,and related genes’expression were analyzed.The results showed that the three exogenous selenium increased the selenium levels in leaves,pericarp and kernel of walnut.They also significantly increased fruit and kernel weights,and kernel linoleic acid,but markedly decreased kernel crude fat and saturated fatty acid.Selenium spraying promoted the absorption of mineral nutrients such as potassium and zinc,but inhibited the absorption of calcium,and had no significant effect on iron and magnesium in the kernel.Three exogenous selenium increased the activities of superoxide dismutase(SOD),peroxidase(POD),ascorbate peroxidase(APX),and catalase(CAT)significantly in the kernel.Except for sodium selenate treatment significantly reduced malondialdehyde(MDA)content in the kernel,the other two selenium sources treatments had no significant effect on MDA and hydrogen peroxide(H2O2)levels.They also increased the expression of JrCu/Zn-SOD,Jr2-Cys POD,JrCyt-APX and JrCAT in kernel,to different extents.These implies that,in the walnut fruit enlargement period,the foliar spraying selenium could increase the selenium content of walnut,affect the mineral nutrient absorption,improve the antioxidant capacity and related genes’expression,and reduce the degree of peroxide,and then improve the quality of fruit.Furthermore,yeast selenium showed the comprehensive effect of the best.
基金supported by the National Key Research and Development Program of China(2019YFD1000103)National Natural Science Foundation of China(31872076)+1 种基金supported by the National Key Research and Development Program of China(2019YFD1000103)National Natural Science Foundation of China(31872076).
文摘The effect of soil nutrient content on fruit yield and fruit quality is very important.To explore the effect of soil nutrients on apple quality we investigated 200 fruit samples from 40 orchards in Feng County,Jiangsu Province.Soil mineral elements and fruit quality were measured.The effect of soil nutrient content on fruit quality was analyzed by artificial neural network(ANN)model.The results showed that the prediction accuracy was highest(R2=0.851,0.847,0.885,0.678 and 0.746)in mass per fruit(MPF),hardness(HB),soluble solids concentrations(SSC),titratable acid concentration(TA)and solid-acid ratio(SSC/TA),respectively.The sensitivity analysis of the prediction model showed that soil available P,K,Ca and Mg contents had the greatest impact on the quality of apple fruit.Response surface method(RSM)was performed to determine the optimum range of the available P,K,Ca,and Mg contents in orchards In Feng County,which were 10∼20 mg⋅kg^(−1),170∼200 mg⋅kg^(−1),1000∼1500 mg⋅kg^(−1),and 80∼200 mg⋅kg^(−1),respectively.The research also concluded that improving the content of available P and available Ca in orchard soil was crucial to improve apple fruit quality in Feng County,Jiangsu Province.
基金supported by the National Key Technology R&D Program(Grant No.2014BAD16B06)the project of the China Agriculture Research System(Grant No.CARS-28)。
文摘Sugar plays an important role in apple fruit development,appearance and quality as well as contributing to a plant’s water stress response.Trehalose and the trehalose biosynthetic metabolic pathways are part of the sugar signaling system in plants,which are important regulator of water stress response in apple.The effect of water stress treatments applied to apple trees and the corresponding effects of ABA on developmental fruit quality were examined for indicators of fruit quality during fruit development.The results indicated that the severe water stress treatment(W2)occurring after the last stage of fruit cell division caused a decrease in the color and size of fruit.The moderate water stress(W1)occurring after the last stage of fruit cell enlargement(S2)caused an increase in the content of fructose and sorbitol while the apple fruit shape was not affected.These changes in sugar are related to the activity of sugar metabolic enzymes.While the enzymatic activity of vacuolar acid invertase(vAINV)was higher,that of sucrose-phosphate synthase(SPS)was lower in water stress treated fruit throughout the developmental period.This indicates that enhanced sucrose degradation and reduced sucrose synthesis leads to an overall reduced sucrose content during times of drought.Thus,water stress reduced sucrose content.Whereas the content of endogenous trehalose and ABA were the highest in water stress treated fruit.A moderate water stress(W1)imposed on apple trees via water restriction(60%–65%of field capacity)after the fruit cell enlargement phase of fruit development yielded sweeter fruit of higher economic value.
基金the National Natural Science Foundation of China(51779161 and 52279041)the National Funds for Distinguished Young Scientists of China(51922072)+1 种基金the Sichuan Science and Technology Program,China(2023YFN0024 and 2023NZZJ0015)the Key Development Project of the Chengdu Science and Technology Plan,China(2022-YF05-01008-SN)。
文摘For a long time,seasonal drought occurs frequently in Southwest China,and the management of water and fertilizer in kiwifruit orchards has no quantitative standards,which seriously affects the yield and quality of kiwifruit.Therefore,the effects of water and fertilizer deficit regulation with drip irrigation(WFDRDI)on the quality of kiwifruit at different growth stages were explored to achieve water and fertilizer saving,and green and efficient production of kiwifruit.We select‘Jin Yan'kiwifruit and set two water deficit levels(W_(D20%)and W_(D40%))and three fertilizer deficit levels(F_(D15%),F_(D30%)and F_(D45%))at bud burst to leafing stage(stageⅠ),flowering to fruit set stage(stageⅡ),fruit expansion stage(stageⅢ)and fruit maturation stage(stageⅣ),respectively,with a full irrigation and fertilization as the control treatment(CK)in 2017and 2018.Results showed that the WFDRDI at stageⅡandⅢhad significant effect on fruit physical quality of kiwifruit,specifically,theⅢ-WD40%F_(D30%)andⅢ-W_(D20%)F_(D45%)treatments significantly increased fruit firmness by 13.62 and 15.59%(P<0.05),respectively;theⅡ-W_(D40%)F_(D15%)andⅢ-W_(D40%)F_(D15%)treatments significantly increased dry matter by 8.19 and 6.47%(P<0.05),respectively;theⅢ-W_(D20%)F_(D15%)treatment significantly increased single fruit weight and fruit volume by 9.33 and 12.65%(P<0.05),respectively;theⅡ-W_(D20%)F_(D15%)treatment significantly increased fruit water content by 1.99%(P<0.05).The WFDRDI had an obvious effect on fruit chemical quality of kiwifruit.TheⅢ-W_(D20%)F_(D45%),Ⅳ-W_(D40%)F_(D15%)andⅣ-W_(D20%)F_(D30%)treatments significantly increased vitamin C(Vc)content by 69.96,36.96 and 34.31%(P<0.05),respectively;theⅢ-W_(D40%)F_(D15%)andⅣ-W_(D40%)F_(D15%)treatments significantly increased total soluble solid(TSS)content by 3.79 and 17.05%(P<0.05),respectively,and significantly increased soluble sugar content by 28.61 and 34.79%(P<0.05),respectively;the contents of fructose,glucose and sucrose also had a significantly increasing trend,which was increased significantly by 5.58–19.63%,40.55–60.36%and 54.03–54.92%in theⅢ-WD40%F_(D15%)andⅣ-W_(D40%)F_(D15%)treatments(P<0.05),respectively;sugar–acid ratio was increased significantly in theⅣ-W_(D40%)F_(D15%)treatment by 64.65%(P<0.05).The degree and duration of water and fertilizer deficit had a comprehensive effect on fruit quality of kiwifruit.The WFDRDI at stageⅡandⅢcontribute to improving fruit physical quality,and the threshold of water and fertilizer deficit were 20 and 15%,respectively;stageⅢandⅣare the critical periods for improving fruit chemical quality by water and fertilizer coupling effect,and the threshold of water and fertilizer deficit were 40 and 15%,respectively.Therefore,aiming at precise water and fertilizer saving,theⅠ-W_(D20%)F_(D30%),Ⅱ-W_(D40%)F_(D15%),Ⅲ-W_(D40%)F_(D15%)andⅣ-W_(D40%)F_(D15%)treatments under WFDRDI during the whole growth period of kiwifruit were the best mode to improve quality and production of kiwifruit.
基金The authors extend their appreciation to the Deanship of Scientific Research at Jouf University for funding this work through Research Grant No.(DSR2020-01-2553).
文摘Olive productivity should be improved through stimulating nutrition,particularly under poor fertility soils.Consequently,the objective of this study was to assess the efficacy of applying organic and bio-fertilizers on the physiological growth,yield and fruit quality of olive trees under newly reclaimed poor-fertility sandy soil in an arid environment.During a field experiment carried out at El-Qantara,North Sinai,Egypt over two consecutive seasons(2019-2020 and 2020-2021),olive Kalamata trees were evaluated under three organic fertilizer treatments alone or in combination with three bio-fertilizers treatments.Organic fertilizer was applied as goat manure(16.8 kg/tree/year),or olive pomace(8.5 kg/tree/year)in mid-December of each season vs.untreated trees.The bio-fertilizers were applied as N-fixing bacteria(150 g/tree)was inculated in early March of each season,or amino acid mixture(1.5%)was applied three times,at 70%of full bloom,21 days after full bloom,and a month later in comparison to a non-fertilized trees(control).The cultivar used was Kalamata,a dual-purpose cultivar for oil and table olives whose value increases when processed as table olives.The results indicated that the goat manure followed by olive pomace significantly enhanced photosynthetic pigments(chlorophyll a,b,and carotenoids),leaf mineral contents(N,P,K,Ca,Mg and Fe),tree canopy volume,number of flowers per inflorescence,number of inflorescences per shoot,initial fruit set,fruit retention.For fruit quality,fruit length and width,fruit weight,and total fruit yield was increased compared to the non-fertilized control.Likewise,The bio-fertilizer N-fixing bacteria followed by the amino acid mixture significantly improved all of the aforementioned parameters.Accordingly,it is recommended,both environmentally and economically to utilize organic and bio-fertizers,particularly goat manure combined with N-fixing bacteria,in low-fertility soil to sustain olive production as well as reducing mineral fertilization.
基金supported by the Major Special Projects and Key R&D Projects in Yunnan Province,China(202102AE090054)the National Natural Science Foundation of China(31925034)+1 种基金the Foundation of Hubei Hongshan Laboratory granted to Dr.Qiang Xu,China(2021hszd016)the Key Project of Hubei Provincial Natural Science Foundation,China(2021CFA017)。
文摘The flesh color of pummelo(Citrus maxima)fruits is highly diverse and largely depends on the level of carotenoids,which are beneficial to human health.It is vital to investigate the regulatory network of carotenoid biosynthesis to improve the carotenoid content in pummelo.However,the molecular mechanism underlying carotenoid accumulation in pummelo is not fully understood.In this study,we identified a novel histone methyltransferase gene,CgSDG40,involved in carotenoid regulation by analyzing the flesh transcriptome of typical white-fleshed pummelo,red-fleshed pummelo and extreme-colored F1 hybrids from a segregated pummelo population.Expression of CgSDG40 corresponded to flesh color change and was highly coexpressed with CgPSY1.Interestingly,CgSDG40 and CgPSY1 are located physically adjacent to each other on the chromosome in opposite directions,sharing a partially overlapping promoter region.Subcellular localization analysis indicated that CgSDG40 localizes to the nucleus.Overexpression of CgSDG40 significantly increased the total carotenoid content in citrus calli relative to that in wild type.In addition,expression of CgPSY1 was significantly activated in overexpression lines relative to wild type.Taken together,our findings reveal a novel histone methyltransferase regulator,CgSDG40,involved in the regulation of carotenoid biosynthesis in citrus and provide new strategies for molecular design breeding and genetic improvement of fruit color and nutritional quality.
文摘Habanero pepper(Capsicum chinense Jacq.)is a crop of economic relevance in the Peninsula of Yucatan.Its fruits have a high level of capsaicinoids compared to peppers grown in other regions of the world,which gives them industrial importance.Soil is an important factor that affects pepper development,nutritional quality,and capsaicinoid content.However,the effect of soil type on fruit development and capsaicinoid metabolism has been little understood.This work aimed to compare the effect of soils with contrasting characteristics,black soil(BS)and red soil(RS),on the expression of genes related to the development of fruits,and capsaicinoid synthesis using a transcriptomic analysis of the habanero pepper fruits.Plants growing in RS had bigger fruits and higher expression of genes related to floral development,fruit abscission,and softening which suggests that RS stimulates fruit development from early stages until maturation stages.Fruits from plants growing in BS had enrichment in metabolic pathways related to growth,sugars,and photosynthesis.Besides,these fruits had higher capsaicinoid accumulation at 25 days post-anthesis,and higher expression of genes related to the branched-chain amino acids metabolism(ketol-acid reductisomerase KARI),pentose phosphate pathway and production of NADPH(glucose-6-phosphate-1-dehydrogenase G6PDH),and proteasome and vesicular traffic in cells(26S proteasome regulatory subunit T4 RPT4),which suggest that BS is better in the early stimulation of pathways related to the nutritional quality and capsaicinoid metabolism in the fruits.
基金We thank the Tomato Genetics Resource Center(http://tgrc.ucdavis.edu)for offering slphyb1cry1 mutant,‘Moneymaker’,and‘Ailsa Craig’seeds.This work was funded by the National Natural Science Foundation of China(32122081,32272698)the National Key Research and Development Program of China(2023YFF1002000)+7 种基金the Natural Science Foundation of Liaoning Province for Excellent Youth(2022-YQ-18)the National Key Research and Development Program of China(2019YFD1000300)the China Agriculture Research System(CARS-23)the National Natural Science Foundation of China(31801904,31991184)the Liao Ning Revitalization Talents Program(XLYC1807020)the Young and Middle-aged Science and Technology Innovation Talent Support Program in Shenyang(RC200449)the Ministry of Science and Technology of the People’s Republic of China(DL2022026004L)the Innovative Research Team(Science and Technology)in University of Henan Province(23IRTSTHN024).
文摘Increasing photosynthesis and light capture offers possibilities for improving crop yield and provides a sustainable way to meet the increasing global demand for food.However,the poor light transmittance of transparent plastic films and shade avoidance at high planting density seriously reduce photosynthesis and alter fruit quality in vegetable crops,and therefore it is important to investigate the mechanisms of light signaling regulation of photosynthesis and metabolism in tomato(Solanum lycopersicum).Here,a combination of red,blue,and white(R1W1B0.5)light promoted the accumulation of chlorophyll,carotenoid,and anthocyanin,and enhanced photosynthesis and electron transport rates by increasing the density of active reaction centers and the expression of the genes LIGHT-HARVESTING COMPLEX B(SlLHCB)and A(SlLHCA),resulting in increased plant biomass.In addition,R1W1B0.5 light induced carotenoid accumulation and fruit ripening by decreasing the expression of LYCOPENEβ-CYCLASE(SlCYCB).Disruption of SlCYCB largely induced fruit lycopene accumulation,and reduced chlorophyll content and photosynthesis in leaves under red,blue,and white light.Molecular studies showed that ELONGATED HYPOCOTYL 5(SlHY5)directly activated SlCYCB,SlLHCB,and SlLHCA expression to enhance chlorophyll accumulation and photosynthesis.Furthermore,R1W1B0.5 light-induced chlorophyll accumulation,photosynthesis,and SlHY5 expression were largely decreased in the slphyb1cry1 mutant.Collectively,R1W1B0.5 light noticeably promoted photosynthesis,biomass,and fruit quality through the photoreceptor(SlPHYB1 and SlCRY1)-SlHY5-SlLHCA/B/SlCYCB module in tomato.Thus,the manipulation of light environments in protected agriculture is a crucial tool to regulate the two vital agronomic traits related to crop production efficiency and fruit nutritional quality in tomato.
基金This research was supported by the National Natural Science Foundation of China(32061143034,32161143028)Tibet Regional Science and Technology Collaborative Innovation Project(QYXTZX-NQ2021-01)Fundamental Research Funds for the Central Universities(lzujbky-2022-ct04).
文摘Background Providing high-quality roughage is crucial for improvement of ruminant production because it is an essential component of their feed.Our previous study showed that feeding bio-fermented rice straw(BF)improved the feed intake and weight gain of sheep.However,it remains unclear why feeding BF to sheep increased their feed intake and weight gain.Therefore,the purposes of this research were to investigate how the rumen micro-biota and serum metabolome are dynamically changing after feeding BF,as well as how their changes influence the feed intake,digestibility,nutrient transport,meat quality and growth performances of sheep.Twelve growing Hu sheep were allocated into 3 groups:alfalfa hay fed group(AH:positive control),rice straw fed group(RS:negative control)and BF fed group(BF:treatment).Samples of rumen content,blood,rumen epithelium,muscle,feed offered and refusals were collected for the subsequent analysis.Results Feeding BF changed the microbial community and rumen fermentation,particularly increasing(P<0.05)relative abundance of Prevotella and propionate production,and decreasing(P<0.05)enteric methane yield.The histomorphology(height,width,area and thickness)of rumen papillae and gene expression for carbohydrate trans-port(MCT1),tight junction(claudin-1,claudin-4),and cell proliferation(CDK4,Cyclin A2,Cyclin E1)were improved(P<0.05)in sheep fed BF.Additionally,serum metabolome was also dynamically changed,which led to up-regulating(P<0.05)the primary bile acid biosynthesis and biosynthesis of unsaturated fatty acid in sheep fed BF.As a result,the higher(P<0.05)feed intake,digestibility,growth rate,feed efficiency,meat quality and mono-unsaturated fatty acid concentration in muscle,and the lower(P<0.05)feed cost per kg of live weight were achieved by feeding BF.Conclusions Feeding BF improved the growth performances and meat quality of sheep and reduced their feed cost.Therefore,bio-fermentation of rice straw could be an innovative way for improving ruminant production with mini-mizing production costs.
基金Supported by Sub-project of National Key Research and Development Program of China(2022YFD2200405-4)。
文摘[Objectives] This study conducted investigations on the climate and soil nutrients of different chestnut orchards in the Yanshan region, selected key ecological factors affecting the growth and fruit quality of chestnuts, and provided a theoretical basis for the cultivation, introduction, and scientific planting of high-quality chestnuts. [Methods] The ‘Yanshanzaofeng’ chestnuts in four orchards located in Qianxi, Qianan, Funing, and Qinglong of Hebei Science and Technology Normal University were selected. The climate and soil nutrient conditions of the four orchards were investigated. Growth indicators such as branch length and diameter, nut weight, and internal quality indicators such as starch, fat, and protein content were analyzed. Principal component analysis was conducted on nine climate factors and twelve soil factors in the four chestnut orchards to identify the most closely related ecological factors influencing chestnut growth and quality. [Results] (i) Different orchards had varying climate conditions, with Qianan orchard having higher rainfall than the others, Funing orchard having the highest number of sunshine hours in the growing season, and Qinglong orchard experiencing the greatest temperature difference during the growing season. (ii) Significant differences were found in soil nutrient content among the orchards, with the coefficient of variation for organic matter and mineral elements ranging from 19.1% (S) to 80.3% (available phosphorus). (iii) The main ecological factor influencing chestnut growth was the photosynthetic factor, while fruit quality was influenced by a combination of climatic factors, photosynthetic factors, and nutrient factors. Key ecological factor indicators included: annual precipitation, annual sunshine hours, growing season precipitation, growing season sunshine hours, soil organic matter, available phosphorus, available potassium, alkali nitrogen, copper, zinc, available boron, and sulfur. Soluble solids were significantly positively correlated with growing season sunshine hours, and fat was significantly positively correlated with available potassium and alkali nitrogen, and significantly positively correlated with available phosphorus. [Conclusions] Orchards with high growing season precipitation, long sunshine hours, and high organic matter and nitrogen-phosphorus-potassium content in the soil are more conducive to promoting the growth of ‘Yanshanzaofeng’ chestnuts and improving fruit quality.
基金This study was supported in part by the National Natural Science Foundation of China(71973136 and 72061147002)the 2115 Talent Development Program of China Agricultural University.
文摘Over the past few decades,the Internet has rapidly diffused across China.The spread of the Internet has had a profound economic and social impact on Chinese rural areas.Existing research shows that Internet access significantly impacts agricultural production and improves smallholder farmers’income.Beyond these,the Internet can affect other dimensions of social welfare.However,research about the impact of Internet access on dietary quality in rural China remains scarce.This study utilizes multi-period panel data from Fixed Observation Point in rural China from 2009 to 2015 to estimate the impact of Internet access on dietary quality and food consumption of rural households and conducts a causal analysis.Regression models with time and household fixed effects allow robust estimation while reducing potential issues of unobserved heterogeneity.The estimates show that Internet access has significantly increased rural household dietary quality(measured by the Chinese Diet Balance Index).Further research finds that Internet access has increased the consumption of animal products,such as aquatic and dairy products.We also examine the underlying mechanisms.Internet access improves dietary quality and food consumption mainly through increasing household income and food expenditure.These results encourage the promotion of Internet access as a valuable tool for nutritional improvements,especially in rural areas.
基金supported by the National Key R&D Program of China(2019YFD1002300).
文摘Jujube fruits usually suffer from physiological disorders or infectious diseases during storage,leading to quality deterioration,softening,or rotting.Therefore,this study aims to investigate the effect of methionine soaking treatment on the postharvest jujube fruit decay rate and storage quality.Methionine treatment significantly reduced the decay rate of postharvest jujube fruit and effectively maintained fruit color and titratable acid.Methionine treatment effectively delayed the decrease of firmness,soluble solids,ascorbic acid,and lignin contents in jujube fruit.Methionine treatment reduced the content of alanine and phenylalanine,and increased the content of glycine in jujube fruit,but displayed no significant effect on total amino acid content.In addition,jujube fruits in the methionine treatment group had a higher total phenolic and flavonoid content and antioxidant capacity,both in free and bound forms.Compared with the control,methionine treatment also significantly increased the content of individual phenolic acid fractions(gallic acid,vanillic acid,and syringic acid)and flavonoid fractions(catechin,epicatechin,rutin)in free form as well as individual phenolic acid fractions in bound form(gallic acid and ferulic acid)in jujube fruits.Overall,this study suggested that the methionine treatment could be used as a prospective preservative to reduce the postharvest decay of jujube fruit and alleviate its nutritional quality deterioration during cold storage at 4°C.
基金supported by the National Natural Science Foundation of China,Nos.82130037(to CH),81971122(to CH),82171323(to WL)the Natural Science Foundation of Jiangsu Province of China,No.BK20201113(to WL)。
文摘The dramatic increase in intracranial pressure after subarachnoid hemorrhage leads to a decrease in cerebral perfusion pressure and a reduction in cerebral blood flow.Mitochondria are directly affected by direct factors such as ischemia,hypoxia,excitotoxicity,and toxicity of free hemoglobin and its degradation products,which trigger mitochondrial dysfunction.Dysfunctional mitochondria release large amounts of reactive oxygen species,inflammatory mediators,and apoptotic proteins that activate apoptotic pathways,further damaging cells.In response to this array of damage,cells have adopted multiple mitochondrial quality control mechanisms through evolution,including mitochondrial protein quality control,mitochondrial dynamics,mitophagy,mitochondrial biogenesis,and intercellular mitochondrial transfer,to maintain mitochondrial homeostasis under pathological conditions.Specific interventions targeting mitochondrial quality control mechanisms have emerged as promising therapeutic strategies for subarachnoid hemorrhage.This review provides an overview of recent research advances in mitochondrial pathophysiological processes after subarachnoid hemorrhage,particularly mitochondrial quality control mechanisms.It also presents potential therapeutic strategies to target mitochondrial quality control in subarachnoid hemorrhage.
基金supported by Liaoning Provincial Department of Educational Annual(2019)Scientific Research Fund Project(LSNZD201903)Shenyang Bureau of Science and Technology Annual(2021)Scientific Research Fund Project(21110319)Shenyang Agricultural University,high-end talent introduction fund project(SYAU20160003).
文摘Edible mushroom sexual spores have been gaining more interest due to their bioactive components and functions.Spore discharge(SD)is an important factor affecting the quality of edible mushrooms.In this review,the bioactive nutrients of sexual spores of edible mushrooms were summarized,the SD mechanism was described,and the relationship between postharvest SD and the quality of edible mushrooms was analyzed.Spores contain various bioactive nutrients that are benefi cial to the human body.Mature mushrooms can actively discharge spores in a process affected by light,relative humidity,and temperature.During storage,the physiological metabolism of spore-bearing gill tissue is vigorous,promoting the release of postharvest spores and changing the nutritional value of fruiting bodies.The flavor of the fruiting bodies also varied signifi cantly during SD.Edible mushroom sexual spores have the potential to become new raw materials for functional food and medical resources.Research on the effect of the mechanism of SD on the quality of edible mushrooms and the development of SD regulation technology may be a new trend in the quality control of edible mushrooms,which will promote the development of the edible mushroom industry.
基金Key Research and Development Program of Hebei Province(19226817D)China Apple Research System(CARS-27)+1 种基金Key Technology R&D Program of Hebei Province(16226312D-2)Basic Research Fund Youth Project of Hebei Academy of Agriculture and Forestry Sciences(2021100102).
文摘[Objectives]The paper was to provide a reference for screening dwarfing rootstock suitable for main spur-type Fuji cultivars in central and southern Hebei Province.[Methods]With spur-type Fuji‘Tianhong 2’as the material,the vegetative growth,yield and fruit quality of 8 different rootstock-scion combinations were compared.[Results]‘Tianhong 2’/SH6 as self-rooted rootstock had large average single fruit weight(256.33 g),large number of fruits per plant(188.68),the highest yield[(3250.08±23.42)kg/667 m ^(2)]and the highest colored area(93.5%),and the soluble solid content reached the requirement of high quality fruit(15.78%).[Conclusions]In central and southern Hebei Province,‘Tianhong 2’grafted on SH6 self-rooted rootstock has moderate growth,high yield and good fruit quality,so it can be considered as the preferred rootstock-scion combination in the local area.
文摘Recently,the fundamental problem with Hybrid Mobile Ad-hoc Net-works(H-MANETs)is tofind a suitable and secure way of balancing the load through Internet gateways.Moreover,the selection of the gateway and overload of the network results in packet loss and Delay(DL).For optimal performance,it is important to load balance between different gateways.As a result,a stable load balancing procedure is implemented,which selects gateways based on Fuzzy Logic(FL)and increases the efficiency of the network.In this case,since gate-ways are selected based on the number of nodes,the Energy Consumption(EC)was high.This paper presents a novel Node Quality-based Clustering Algo-rithm(NQCA)based on Fuzzy-Genetic for Cluster Head and Gateway Selection(FGCHGS).This algorithm combines NQCA with the Improved Weighted Clus-tering Algorithm(IWCA).The NQCA algorithm divides the network into clusters based upon node priority,transmission range,and neighbourfidelity.In addition,the simulation results tend to evaluate the performance effectiveness of the FFFCHGS algorithm in terms of EC,packet loss rate(PLR),etc.
基金financially supported by the Construction Program for Chongqing's Distinctive“Wushancuili”Industry(Grant No.4322200370)Strategic Cooperation Project of Chongqing Municipality and Chinese Academy of Agricultural Sciences(Grant No.4322300181)Fundamental Research Funds for Central Universities-Talent induction project(Grant Nos.SWU-KR22001,SWU-KQ22070)。
文摘Chinese plum(Prunus salicina Lindl.)originates from China and makes a large contribution to the global production of plums.The P.salicina‘Wushancuili'has a green coloration and high fruit quality and is economically important in eliminating poverty and protecting ecology in the Yangtze River Three Gorges Reservoir.However,rain-induced cracking(rain-cracking,literally skin cracking caused by rain)is a limitation to‘Wushancuili'fruit production and causes severe losses.This study reported a high-quality‘Wushancuili'genome assembly consisting of a 302.17-Mb sequence with eight pseudo-chromosomes and a contig N50 of 23.59 Mb through the combination of Illumina sequencing,Pacific Biosciences HiFiⅢsequencing,and high-throughput chromosome conformation capture technology.A total of 25109 protein-coding genes are predicted and 54.17%of the genome is composed of repetitive sequences.‘Wushancuili'underwent a remarkable orthoselection during evolution.Gene identification revealed that loss-of-function in four core MYB10 genes results in the anthocyanin deficiency and absence of red color,revealing the green coloration due to the residual high chlorophyll in fruit skin.Besides,the occurrence of cracking is assumed to be closely associated with cell wall modification and frequently rain-induced pathogen enrichment through transcriptomic analysis.The loss of MYB10 genes might render fruit more susceptible to pathogen-mediated cracking by weakening the epidermal strength and reactive oxygen species(ROS)scavenging.Our findings provided fundamental knowledge regarding fruit coloration and rain-cracking and will facilitate genetic improvement and cultivation management in Chinese plums.