[目的 ]扩增和克隆猪带绦虫囊尾蚴 Ag B基因的 c DNA编码区。 [方法 ]提取猪囊尾蚴总 RNA中 ,利用 RT- PCR技术扩增出 Ag B基因 c DNA编码区 ,然后将其克隆到载体 p UC118中进行序列分析。 [结果 ]PCR反应产物为单一条带 ,大小为 2 .6 k...[目的 ]扩增和克隆猪带绦虫囊尾蚴 Ag B基因的 c DNA编码区。 [方法 ]提取猪囊尾蚴总 RNA中 ,利用 RT- PCR技术扩增出 Ag B基因 c DNA编码区 ,然后将其克隆到载体 p UC118中进行序列分析。 [结果 ]PCR反应产物为单一条带 ,大小为 2 .6 kb。测序结果与澳大利亚株猪囊尾蚴 Ag B序列有 99.8%的同源性 ,二者的氨基酸序列有 99.3%的同源性。 [结论 ]成功地克隆了猪囊尾蚴 Ag B基因 c DNA编码区。展开更多
A cDNA encoding a protein is composed of coding region and nontranslated region (NTR). There are two parts of NTR. i.e. 5’ NTR or 3’ NTR, upstream or downstream the coding region respectively. According to neutral t...A cDNA encoding a protein is composed of coding region and nontranslated region (NTR). There are two parts of NTR. i.e. 5’ NTR or 3’ NTR, upstream or downstream the coding region respectively. According to neutral theory of molecular evolution, the following expectations might be true at cDNA level: (i)evolutionary rate of NTR is higher than that of coding region; (ii) synonymous substitutions in coding region evolution are more than missense substitutions; (iii) transition substitutions are more than展开更多
文摘[目的 ]扩增和克隆猪带绦虫囊尾蚴 Ag B基因的 c DNA编码区。 [方法 ]提取猪囊尾蚴总 RNA中 ,利用 RT- PCR技术扩增出 Ag B基因 c DNA编码区 ,然后将其克隆到载体 p UC118中进行序列分析。 [结果 ]PCR反应产物为单一条带 ,大小为 2 .6 kb。测序结果与澳大利亚株猪囊尾蚴 Ag B序列有 99.8%的同源性 ,二者的氨基酸序列有 99.3%的同源性。 [结论 ]成功地克隆了猪囊尾蚴 Ag B基因 c DNA编码区。
文摘A cDNA encoding a protein is composed of coding region and nontranslated region (NTR). There are two parts of NTR. i.e. 5’ NTR or 3’ NTR, upstream or downstream the coding region respectively. According to neutral theory of molecular evolution, the following expectations might be true at cDNA level: (i)evolutionary rate of NTR is higher than that of coding region; (ii) synonymous substitutions in coding region evolution are more than missense substitutions; (iii) transition substitutions are more than
基金国家自然科学基金(the National Natural Science Foundation of China under Grant No.60602066)广东省科技计划项目(the Key Technolo-gies R&D Program of Guangdong Province,China under Grant No.2006B36430001)
