To understand the function of porcine adipocyte-special membrane protein （PAMP） gene and the difference of fat deposition ability among various lean pig breeds, a full-length porcine adipocyte-special membrane prote...To understand the function of porcine adipocyte-special membrane protein （PAMP） gene and the difference of fat deposition ability among various lean pig breeds, a full-length porcine adipocyte-special membrane protein （PAMP） gene was successfully amplified using reverse transcription polymerase chain reaction （RT-PCR） and 5＇-rapid amplification of cDNA end （5＇-RACE）. The open reading frame was 1 587 bp encoding 529 amino acids. The nucleotide sequence of the fulllength PAMP gene was deposited in the GenBank under the accession number EF433431. The PAMP gene mRNA expression was analyzed on three lean pig breeds by quantitative reverse transcription polymerase chain reaction （QRTPCR）. The PAMP gene mRNA levels in YHM （Yorkshire × Hampshire × Meishan） pig and DLY （Duroc × Landrance × Yorkshire） pig were about 0.82 and 0.38 times of that in SW （Shanxi-White） pig, respectively.展开更多
A full-length cDNA of proteinase inhibitor gene with completed open reading frame of 116 amino acids was cloned from Ralstonia solanacearum （Rs） resistant potato leaves using the rapid amplification of cDNA ends （R...A full-length cDNA of proteinase inhibitor gene with completed open reading frame of 116 amino acids was cloned from Ralstonia solanacearum （Rs） resistant potato leaves using the rapid amplification of cDNA ends （RACE） method and designated as StPI. BLAST search against NCBI showed that the StPI gene shared 89% identity with potato proteinase inhibitor I precursor in nucleotide and 74% in amino acid. Analysis of semi-quantitative RT-PCR indicated that this gene was induced by Rs as well as up-regulated by jasmonic acid （JA）. The StPI gene expression reached the highest level during 6-12 h post Rs-inoculation or JA-treatment, and then leveled off. Moreover, this gene was strongly induced by JA and its mRNA accumulation increased more quickly than that of Rs-inoculation. The StPI gene may play a role in potato resistance against Rs. The induction of StPI by Rs invasion may have a similar signal transduction pathway with JA treatment.展开更多
Background This investigation was undertaken to obtain differentially expressed genes related to human glioma using cDNA microarray and the characterization of one novel full-length gene. Methods Total RNA was extract...Background This investigation was undertaken to obtain differentially expressed genes related to human glioma using cDNA microarray and the characterization of one novel full-length gene. Methods Total RNA was extracted from human glioma tissues and normal brain tissues, and mRNA was used to make probes. After hybridization and washing, the results were scanned using a computer system. The gene named 681F05 clone was an expressed gene to human glioma through four-time hybridization and scanning. Subsequently northern blot analysis was performed by northern blot, 5’RACE and bioinformatics. Results Fifteen differentially expressed genes to human glioma were obtained through four-time hybridization and scanning. Northern blot analysis confirmed that 681F05 clone was low-expressed in human brain tissues and over-expressed in human glioma tissues. The analysis of BLASTn and BLASTx showed that 681F05 clone is two cDNA clones encoding two novel proteins that are highly identified to the cyclophilin isoform 10 of C. Elgans, respectively. Sequence analysis revealed the two cDNA clones are two different splicing variants of a novel cycophilin-like gene (PPIL3a and PPIL3b).Conclusions cDNA microarray technology can be successfully used to identify differentially expressed genes. The novel full-length gene of human PPIL3 may be correlated with the formation of human glioma.展开更多
基金the National Natural Science Foundation of China (20011089)the Fi-nance Department Achievement Transformation Project of Shanxi Province in China (2005)
文摘To understand the function of porcine adipocyte-special membrane protein （PAMP） gene and the difference of fat deposition ability among various lean pig breeds, a full-length porcine adipocyte-special membrane protein （PAMP） gene was successfully amplified using reverse transcription polymerase chain reaction （RT-PCR） and 5＇-rapid amplification of cDNA end （5＇-RACE）. The open reading frame was 1 587 bp encoding 529 amino acids. The nucleotide sequence of the fulllength PAMP gene was deposited in the GenBank under the accession number EF433431. The PAMP gene mRNA expression was analyzed on three lean pig breeds by quantitative reverse transcription polymerase chain reaction （QRTPCR）. The PAMP gene mRNA levels in YHM （Yorkshire × Hampshire × Meishan） pig and DLY （Duroc × Landrance × Yorkshire） pig were about 0.82 and 0.38 times of that in SW （Shanxi-White） pig, respectively.
文摘A full-length cDNA of proteinase inhibitor gene with completed open reading frame of 116 amino acids was cloned from Ralstonia solanacearum （Rs） resistant potato leaves using the rapid amplification of cDNA ends （RACE） method and designated as StPI. BLAST search against NCBI showed that the StPI gene shared 89% identity with potato proteinase inhibitor I precursor in nucleotide and 74% in amino acid. Analysis of semi-quantitative RT-PCR indicated that this gene was induced by Rs as well as up-regulated by jasmonic acid （JA）. The StPI gene expression reached the highest level during 6-12 h post Rs-inoculation or JA-treatment, and then leveled off. Moreover, this gene was strongly induced by JA and its mRNA accumulation increased more quickly than that of Rs-inoculation. The StPI gene may play a role in potato resistance against Rs. The induction of StPI by Rs invasion may have a similar signal transduction pathway with JA treatment.
文摘Background This investigation was undertaken to obtain differentially expressed genes related to human glioma using cDNA microarray and the characterization of one novel full-length gene. Methods Total RNA was extracted from human glioma tissues and normal brain tissues, and mRNA was used to make probes. After hybridization and washing, the results were scanned using a computer system. The gene named 681F05 clone was an expressed gene to human glioma through four-time hybridization and scanning. Subsequently northern blot analysis was performed by northern blot, 5’RACE and bioinformatics. Results Fifteen differentially expressed genes to human glioma were obtained through four-time hybridization and scanning. Northern blot analysis confirmed that 681F05 clone was low-expressed in human brain tissues and over-expressed in human glioma tissues. The analysis of BLASTn and BLASTx showed that 681F05 clone is two cDNA clones encoding two novel proteins that are highly identified to the cyclophilin isoform 10 of C. Elgans, respectively. Sequence analysis revealed the two cDNA clones are two different splicing variants of a novel cycophilin-like gene (PPIL3a and PPIL3b).Conclusions cDNA microarray technology can be successfully used to identify differentially expressed genes. The novel full-length gene of human PPIL3 may be correlated with the formation of human glioma.
