Background: The skin serves as the first line of defense for the human body. Direct sunlight contains damaging radiations that can speed up the ageing process of the skin, resulting in wrinkles, leathery skin, dark pa...Background: The skin serves as the first line of defense for the human body. Direct sunlight contains damaging radiations that can speed up the ageing process of the skin, resulting in wrinkles, leathery skin, dark patches, and solar elastosis. Objectives: To evaluate the effect of multiple solar irradiation related factors at the protein level in human dermal fibroblast (HDF). The overall effect of individual solar irradiations such as Infrared A (IRA), blue light (BL), UVA, and UVB on HDF cells and the extent of molecular level aberrations to be assessed and compared against each. Methods: Label-free quantitative proteomics (MS/MS) approach has been adopted in this study to observe the protein level changes induced in the HDF cells through various exposures of full light sources. Following that, downstream insilico analysis has been carried out. Results: In this study, it is demonstrated all the four different solar irradiations significantly contribute to the molecular degeneration of skin cells through various mechanisms. This study confirms that BL down-regulates DNA repair proteins and the skin cells-HDF stimulate the histone proteins as a response mechanism to maintain the chromosomal integrity. Conclusions: The proteomics experiment carried out in the current study intends to support the future sun care products based on full light protection technology that can be custom designed to provide complete protection from the solar radiation. Similar technology could enhance the further investigations for deeper understanding of induction, mode of action, and prevention of skin damage from extensive solar irradiation.展开更多
In order to obtain a deeper understanding of the behavior of the structure under high wind load,this paper conducted an experimental study on a full-scale L-shaped single story light frame wood house under the uniform...In order to obtain a deeper understanding of the behavior of the structure under high wind load,this paper conducted an experimental study on a full-scale L-shaped single story light frame wood house under the uniform lateral load simulated using a gasbag.The investigation involved the performance of light frame wood structure after it experienced the repeated cyclic lateral wind load as well as the performance of the structure under the ultimate lateral load.Then,the study verified that light frame wood structure can resist repeated cyclic wind loads without observable degradation in stiffness during the anticipated serve life,and recommended shear wall percent drift restriction for lateral wind load design of wood structure in serviceability limit states is 1/400 drift,and in ultimate limit states is 1/80 drift.The conclusions of this paper can be benefit for the engineering practice of the light frame wood structures in high wind load regions.展开更多
文摘Background: The skin serves as the first line of defense for the human body. Direct sunlight contains damaging radiations that can speed up the ageing process of the skin, resulting in wrinkles, leathery skin, dark patches, and solar elastosis. Objectives: To evaluate the effect of multiple solar irradiation related factors at the protein level in human dermal fibroblast (HDF). The overall effect of individual solar irradiations such as Infrared A (IRA), blue light (BL), UVA, and UVB on HDF cells and the extent of molecular level aberrations to be assessed and compared against each. Methods: Label-free quantitative proteomics (MS/MS) approach has been adopted in this study to observe the protein level changes induced in the HDF cells through various exposures of full light sources. Following that, downstream insilico analysis has been carried out. Results: In this study, it is demonstrated all the four different solar irradiations significantly contribute to the molecular degeneration of skin cells through various mechanisms. This study confirms that BL down-regulates DNA repair proteins and the skin cells-HDF stimulate the histone proteins as a response mechanism to maintain the chromosomal integrity. Conclusions: The proteomics experiment carried out in the current study intends to support the future sun care products based on full light protection technology that can be custom designed to provide complete protection from the solar radiation. Similar technology could enhance the further investigations for deeper understanding of induction, mode of action, and prevention of skin damage from extensive solar irradiation.
基金Sponsored by the National Natural Science Foundation of China(Grant No. 50508012)
文摘In order to obtain a deeper understanding of the behavior of the structure under high wind load,this paper conducted an experimental study on a full-scale L-shaped single story light frame wood house under the uniform lateral load simulated using a gasbag.The investigation involved the performance of light frame wood structure after it experienced the repeated cyclic lateral wind load as well as the performance of the structure under the ultimate lateral load.Then,the study verified that light frame wood structure can resist repeated cyclic wind loads without observable degradation in stiffness during the anticipated serve life,and recommended shear wall percent drift restriction for lateral wind load design of wood structure in serviceability limit states is 1/400 drift,and in ultimate limit states is 1/80 drift.The conclusions of this paper can be benefit for the engineering practice of the light frame wood structures in high wind load regions.
