With the continuous advancement of science and technology and the advent of the digital era,blockchain technology is increasingly demonstrating its great potential across various industries due to its unique features ...With the continuous advancement of science and technology and the advent of the digital era,blockchain technology is increasingly demonstrating its great potential across various industries due to its unique features of decentralization,transparency,and immutability.In particular,in the cold chain logistics of agricultural products,the application of blockchain technology can not only enhance logistics efficiency but also ensure food safety and boost consumer trust[1].This paper first outlines the advantages of blockchain technology in cold chain logistics for agricultural products,and then clarifies the application pathways of blockchain technology in this field,providing a reference for relevant researchers.展开更多
AIM:To develop a real-time polymerase chain reaction(PCR) method to detect and quantify Campylobacter jejuni(C.jejuni) from stool specimens.METHODS:Primers and a probe for real-time PCR were designed based on the spec...AIM:To develop a real-time polymerase chain reaction(PCR) method to detect and quantify Campylobacter jejuni(C.jejuni) from stool specimens.METHODS:Primers and a probe for real-time PCR were designed based on the specific DNA sequence of the hipO gene in C.jejuni.The specificity of the primers and probe were tested against a set of Campylobacter spp.and other enteric pathogens.The optimal PCR conditions were determined by testing a series of conditions with standard a C.jejuni template.The detection limits were obtained using purified DNA from bacterial culture and extracted DNA from the stool specimen.Two hundred and forty-two specimens were analyzed for the presence of C.jejuni by direct bacterial culture and real-time PCR.RESULTS:The optimal PCR system was determined using reference DNA templates,1 × uracil-DNA glycosylase,3.5 mmol/L MgCl 2,1.25 U platinum Taq polymerase,0.4 mmol/L PCR nucleotide mix,0.48 μmol/L of each primer,0.2 μmol/L of probe and 2 μL of DNA template in a final volume of 25 μL.The PCR reaction was carried as follows:95 ℃ for 4 min,followed by 45 cycles of 10 s at 95 ℃ and 30 s at 59 ℃.The detection limit was 4.3 CFU/mL using purified DNA from bacterial culture and 10 3 CFU/g using DNA from stool specimens.Twenty(8.3%,20/242) C.jejuni strains were isolated from bacterial culture,while 41(16.9%,41/242) samples were found to be positive by realtime PCR.DNA sequencing of the PCR product indicated the presence of C.jejuni in the specimen.One mixed infection of C.jejuni and Salmonella was detected in one specimen and the PCR test for this specimen was positive.CONCLUSION:The sensitivity of detection of C.jejuni from stool specimens was much higher using this PCR assay than using the direct culture method.展开更多
SINOPEC is an integrated energy and chemical company engaging in upstream,midstream and downstream operations. The business of SINOPEC covers E&P of oil and gas, refining,chemical production, as well as sales and ...SINOPEC is an integrated energy and chemical company engaging in upstream,midstream and downstream operations. The business of SINOPEC covers E&P of oil and gas, refining,chemical production, as well as sales and marketing of refined oil products. In the field of oil andnatural gas exploration and development, there are 13 upstream subsidiaries in SINOPEC. By the endof 2003, the proven reserves of crude oil were 3,257 mmbbls, and natural gas reserves totaled2,887.6 bcf. The oil and gas production in 2003 included 270.96 mmbbls of oil and 187.7 bcf ofnatural gas. In the area of middle-stream operation, SINOPEC has 33 refineries. The total capacityof crude distillation is 153.9 million tons/year, about 51% of the total distillation capacity inChina. In 2003, 123.5 million tons of crude oil was processed, around 53% of total crude processedin China.展开更多
基金2021 Fujian Provincial Education and Research Project for Middle and Young Teachers(Science and Technology)“Research on Optimization of Agricultural Product Supply Chain Information Based on Blockchain Technology”(Project No.JAT210628)。
文摘With the continuous advancement of science and technology and the advent of the digital era,blockchain technology is increasingly demonstrating its great potential across various industries due to its unique features of decentralization,transparency,and immutability.In particular,in the cold chain logistics of agricultural products,the application of blockchain technology can not only enhance logistics efficiency but also ensure food safety and boost consumer trust[1].This paper first outlines the advantages of blockchain technology in cold chain logistics for agricultural products,and then clarifies the application pathways of blockchain technology in this field,providing a reference for relevant researchers.
基金Supported by The General Program of National Natural Science Foundation of China,No.81271789the Major State Basic Research Development Program,No.2013CB127204
文摘AIM:To develop a real-time polymerase chain reaction(PCR) method to detect and quantify Campylobacter jejuni(C.jejuni) from stool specimens.METHODS:Primers and a probe for real-time PCR were designed based on the specific DNA sequence of the hipO gene in C.jejuni.The specificity of the primers and probe were tested against a set of Campylobacter spp.and other enteric pathogens.The optimal PCR conditions were determined by testing a series of conditions with standard a C.jejuni template.The detection limits were obtained using purified DNA from bacterial culture and extracted DNA from the stool specimen.Two hundred and forty-two specimens were analyzed for the presence of C.jejuni by direct bacterial culture and real-time PCR.RESULTS:The optimal PCR system was determined using reference DNA templates,1 × uracil-DNA glycosylase,3.5 mmol/L MgCl 2,1.25 U platinum Taq polymerase,0.4 mmol/L PCR nucleotide mix,0.48 μmol/L of each primer,0.2 μmol/L of probe and 2 μL of DNA template in a final volume of 25 μL.The PCR reaction was carried as follows:95 ℃ for 4 min,followed by 45 cycles of 10 s at 95 ℃ and 30 s at 59 ℃.The detection limit was 4.3 CFU/mL using purified DNA from bacterial culture and 10 3 CFU/g using DNA from stool specimens.Twenty(8.3%,20/242) C.jejuni strains were isolated from bacterial culture,while 41(16.9%,41/242) samples were found to be positive by realtime PCR.DNA sequencing of the PCR product indicated the presence of C.jejuni in the specimen.One mixed infection of C.jejuni and Salmonella was detected in one specimen and the PCR test for this specimen was positive.CONCLUSION:The sensitivity of detection of C.jejuni from stool specimens was much higher using this PCR assay than using the direct culture method.
文摘SINOPEC is an integrated energy and chemical company engaging in upstream,midstream and downstream operations. The business of SINOPEC covers E&P of oil and gas, refining,chemical production, as well as sales and marketing of refined oil products. In the field of oil andnatural gas exploration and development, there are 13 upstream subsidiaries in SINOPEC. By the endof 2003, the proven reserves of crude oil were 3,257 mmbbls, and natural gas reserves totaled2,887.6 bcf. The oil and gas production in 2003 included 270.96 mmbbls of oil and 187.7 bcf ofnatural gas. In the area of middle-stream operation, SINOPEC has 33 refineries. The total capacityof crude distillation is 153.9 million tons/year, about 51% of the total distillation capacity inChina. In 2003, 123.5 million tons of crude oil was processed, around 53% of total crude processedin China.
