An open-top chamber experiment was conducted at the University of Michigan Biological Station near Pellston, Michigan, USA, to study the effects of soil fertility and CO2 on leaf, stem and root dark respiration (Rd) o...An open-top chamber experiment was conducted at the University of Michigan Biological Station near Pellston, Michigan, USA, to study the effects of soil fertility and CO2 on leaf, stem and root dark respiration (Rd) of Populus tremuloides. Overall, area-based day-time leaf Rd (Rda) was significantly greater at elevated than at ambient CO2 in high-fertility soil, but not in low-fertility soil. Mass-based leaf Rd (Rdm) was overall greater for high- than for low-fertility soil grown trees at elected, but not at ambient CO2. Nighttime leaf Rd. and Rdm were unthected by soil fertility or CO2, nor was stem Rda, which ranged from 1.0 to 1.4 μmol m-2 s-1 in the spring and 3.5 to 4.5 μmol m-2 s-1 in the summer. Root Rda. was significantly higher in high- than in low-fertility soil, but was unaffected by CO2. Since biomass production of P. tremuloides will be significantly greater at elevated CO2 while specific Rd will either increase or remain unchanged, we predict that carbon loss to the atmosphere through respiration from this ecologically important species would increase at higher CO2. Soil fertility would also interact with elevated CO2 in affecting the carbon flow in the plant-soil-air system.展开更多
In this study,effects of temperature,light and their interactions on allelopathic effects and the functional traits specific leaf area(SLA)and stem mass fraction(SMF)of different allelopathic potential rice accessions...In this study,effects of temperature,light and their interactions on allelopathic effects and the functional traits specific leaf area(SLA)and stem mass fraction(SMF)of different allelopathic potential rice accessions at different growth stages were analyzed.The main results were as follows:Allelopathic responses to temperature and light varied with different allelopathic potential rice accessions at different growth stages.With the rise of temperature and the extension of photoperiod,allelopathic effect increased firstly and then decreased at 2–3 leaf stage,but increased constantly at the 4–5 and 7–8 leaf stages in strong allelopathic rice accessions[O.longistaminata,F1(O.longistaminata×RD23),F2(RL159 and RL169)].Temperature had significant impact on allelopathic effect without considering light factors,but light showed little effect on rice allelopathy at the same temperature conditions.The greatest allelopathic effect was attained with moderate temperature and long photoperiod at 2–3 leaf stage in strong allelopathic rice accessions,but all the rice accessions showed weak allelopathic effects at the low temperature condition(15oC/10oC),and the influence of different factors on allelopathy followed a general trend as temperature>leaf stage>light,indicating that among the multiple factors impacting rice allelopathy,temperature was the main factor.Allelopathic characteristics of F1 and F2 to various temperature and light were similar to O.longistaminata,showing that allelopathic genes from wild rice can be expressed in its descendants.Temperature and light also had significant effects on SLA and SMF,and rice allelopathy was closely correlative to SLA in strong allelopathic rice accessions at the 4–5 and 7–8 leaf stages,but there was no correlation between rice allelopathy and SMF at different growth stages.These results suggested that rice adjust the relationship between allelopathy and SLA and adapt to the varied environments,and that high temperature and long photoperiod can enhance rice allelopathic activity.展开更多
DNA methylation plays a crucial role in regulating plant development and tissue differentiation. In this study, we compared the methylation levels in leaf, root, and stem in Chamaedorea elegans by using the technique ...DNA methylation plays a crucial role in regulating plant development and tissue differentiation. In this study, we compared the methylation levels in leaf, root, and stem in Chamaedorea elegans by using the technique of methylation-sensitive amplified fragment length polymorphism AFLP. Over 19% （42/220） bases were uniformly methy- lated in these tissues. The percentages of polymorphism resulting from varied methylation in mature leaf （L1）, young leaf （L2）, baby leaf （L3）, stem （S）, young root （R1） and lignified root （R2） were 29.5%, 29.0%, 27.1%, 30.7%, 63.0% and 28.3%, respectively. The numbers of poly- morphic loci detected in the leaves of three developmental stages were similar, ranging from 20 to 30. In contrast, roots at the two developmental stages differed greatly, with 145 polymorphic loci detected in R1 and 27 in R2. Our results suggest that the methylation level in leaves slightly increases with aging, while that in roots decreases dramatically with aging.展开更多
基金the National institute for Global Environmental Change (DOENIGEC), Program for Ecosystem Research (DOE-PER Grant D E- FG O Z-9
文摘An open-top chamber experiment was conducted at the University of Michigan Biological Station near Pellston, Michigan, USA, to study the effects of soil fertility and CO2 on leaf, stem and root dark respiration (Rd) of Populus tremuloides. Overall, area-based day-time leaf Rd (Rda) was significantly greater at elevated than at ambient CO2 in high-fertility soil, but not in low-fertility soil. Mass-based leaf Rd (Rdm) was overall greater for high- than for low-fertility soil grown trees at elected, but not at ambient CO2. Nighttime leaf Rd. and Rdm were unthected by soil fertility or CO2, nor was stem Rda, which ranged from 1.0 to 1.4 μmol m-2 s-1 in the spring and 3.5 to 4.5 μmol m-2 s-1 in the summer. Root Rda. was significantly higher in high- than in low-fertility soil, but was unaffected by CO2. Since biomass production of P. tremuloides will be significantly greater at elevated CO2 while specific Rd will either increase or remain unchanged, we predict that carbon loss to the atmosphere through respiration from this ecologically important species would increase at higher CO2. Soil fertility would also interact with elevated CO2 in affecting the carbon flow in the plant-soil-air system.
基金financially supported by the National Natural Science Foundation of China (Grant No. 31260453)the Science and Technology Innovation Talents Project of Yunnan Province, China (Grant No. 2014HB039)
文摘In this study,effects of temperature,light and their interactions on allelopathic effects and the functional traits specific leaf area(SLA)and stem mass fraction(SMF)of different allelopathic potential rice accessions at different growth stages were analyzed.The main results were as follows:Allelopathic responses to temperature and light varied with different allelopathic potential rice accessions at different growth stages.With the rise of temperature and the extension of photoperiod,allelopathic effect increased firstly and then decreased at 2–3 leaf stage,but increased constantly at the 4–5 and 7–8 leaf stages in strong allelopathic rice accessions[O.longistaminata,F1(O.longistaminata×RD23),F2(RL159 and RL169)].Temperature had significant impact on allelopathic effect without considering light factors,but light showed little effect on rice allelopathy at the same temperature conditions.The greatest allelopathic effect was attained with moderate temperature and long photoperiod at 2–3 leaf stage in strong allelopathic rice accessions,but all the rice accessions showed weak allelopathic effects at the low temperature condition(15oC/10oC),and the influence of different factors on allelopathy followed a general trend as temperature>leaf stage>light,indicating that among the multiple factors impacting rice allelopathy,temperature was the main factor.Allelopathic characteristics of F1 and F2 to various temperature and light were similar to O.longistaminata,showing that allelopathic genes from wild rice can be expressed in its descendants.Temperature and light also had significant effects on SLA and SMF,and rice allelopathy was closely correlative to SLA in strong allelopathic rice accessions at the 4–5 and 7–8 leaf stages,but there was no correlation between rice allelopathy and SMF at different growth stages.These results suggested that rice adjust the relationship between allelopathy and SLA and adapt to the varied environments,and that high temperature and long photoperiod can enhance rice allelopathic activity.
基金supported by the Natural Science Foundation of China (30800879)
文摘DNA methylation plays a crucial role in regulating plant development and tissue differentiation. In this study, we compared the methylation levels in leaf, root, and stem in Chamaedorea elegans by using the technique of methylation-sensitive amplified fragment length polymorphism AFLP. Over 19% （42/220） bases were uniformly methy- lated in these tissues. The percentages of polymorphism resulting from varied methylation in mature leaf （L1）, young leaf （L2）, baby leaf （L3）, stem （S）, young root （R1） and lignified root （R2） were 29.5%, 29.0%, 27.1%, 30.7%, 63.0% and 28.3%, respectively. The numbers of poly- morphic loci detected in the leaves of three developmental stages were similar, ranging from 20 to 30. In contrast, roots at the two developmental stages differed greatly, with 145 polymorphic loci detected in R1 and 27 in R2. Our results suggest that the methylation level in leaves slightly increases with aging, while that in roots decreases dramatically with aging.
