Owing to their potential applications,as well as their structural diversity,the discovery of novel secondary metabolites from insect-associated fungi has been of interest to researchers in recent years.The aim of this...Owing to their potential applications,as well as their structural diversity,the discovery of novel secondary metabolites from insect-associated fungi has been of interest to researchers in recent years.The aim of this study was therefore to estimate the diversity of fungi associated with fungus-growing termites and bioprospecting these for potential secondary metabolites.In total,18 fungal species were isolated and described from the gut and comb of Macrotermes barneyi based on 18S ribosomal DNA gene sequence analysis.Antimicrobial activity assays were carried out on all the known fungi,and nine isolates were recorded as active against pathogenic fungi.Xylaria escharoidea,the best performing isolate,was grown at laboratory scale and 4,8-dihydroxy-3,4-dihydronaphthalen-l(2H)was isolated and characterized.The minimum inhibitory concentration of this isolated compound against tested pathogenic organisms was found to be 6.25 fig.In addition,molecular docking studies have revealed that 4,8-dihydroxy-3,4-dihydronaphthalen-l(2H)is a prominent antibacterial agent with a marked interaction with key residues on protein A(agrAc)that regulates the accessory gene.The findings of this study support the drug discovery of antimicrobial properties in insect-associated fungi,which may lead to novel secondary metabolites.展开更多
Ophiostomatalean fungi may facilitate bark beetle colonization and reproduction.In the present study of the fungal community associated with bark beetle species belonging to Tomicus in Yunnan,China,six ophiostomatalea...Ophiostomatalean fungi may facilitate bark beetle colonization and reproduction.In the present study of the fungal community associated with bark beetle species belonging to Tomicus in Yunnan,China,six ophiostomatalean fungi(Ophiostoma canum,O.ips,O.tingens,Leptographium yunnanense,Leptographium sp.1 and Leptographium sp.2)were isolated from the beetles or their galleries;O.canum was the most common fungal species.The distribution of O.canum was associated with stands heavily damaged by Tomicus species and a higher percentage of valid galleries of Tomicus yunnanensis and T.minor in Yunnan pine(Pinus yunnanensis).After inoculation of Yunnan pine with the fungus,a phloem reaction zone formed and monoterpenes accumulated in the phloem.These results suggested that O.canum was pathogenic to Yunnan pine and that the wide distribution of the fungus might be benefi cial to reproduction of pine shoot beetles in Yunnan pine.However,because the reaction zone and monoterpene accumulation were mild,fungal damage of Yunnan pine might be limited.A more integrated study considering all the fungal species should be done to better understand the interactions among bark beetles,blue-stain fungi,and the tree hosts in the region.展开更多
基金This study was supported by grants from the National Natural Science Foundation of China(31272370,31970119).
文摘Owing to their potential applications,as well as their structural diversity,the discovery of novel secondary metabolites from insect-associated fungi has been of interest to researchers in recent years.The aim of this study was therefore to estimate the diversity of fungi associated with fungus-growing termites and bioprospecting these for potential secondary metabolites.In total,18 fungal species were isolated and described from the gut and comb of Macrotermes barneyi based on 18S ribosomal DNA gene sequence analysis.Antimicrobial activity assays were carried out on all the known fungi,and nine isolates were recorded as active against pathogenic fungi.Xylaria escharoidea,the best performing isolate,was grown at laboratory scale and 4,8-dihydroxy-3,4-dihydronaphthalen-l(2H)was isolated and characterized.The minimum inhibitory concentration of this isolated compound against tested pathogenic organisms was found to be 6.25 fig.In addition,molecular docking studies have revealed that 4,8-dihydroxy-3,4-dihydronaphthalen-l(2H)is a prominent antibacterial agent with a marked interaction with key residues on protein A(agrAc)that regulates the accessory gene.The findings of this study support the drug discovery of antimicrobial properties in insect-associated fungi,which may lead to novel secondary metabolites.
文摘Ophiostomatalean fungi may facilitate bark beetle colonization and reproduction.In the present study of the fungal community associated with bark beetle species belonging to Tomicus in Yunnan,China,six ophiostomatalean fungi(Ophiostoma canum,O.ips,O.tingens,Leptographium yunnanense,Leptographium sp.1 and Leptographium sp.2)were isolated from the beetles or their galleries;O.canum was the most common fungal species.The distribution of O.canum was associated with stands heavily damaged by Tomicus species and a higher percentage of valid galleries of Tomicus yunnanensis and T.minor in Yunnan pine(Pinus yunnanensis).After inoculation of Yunnan pine with the fungus,a phloem reaction zone formed and monoterpenes accumulated in the phloem.These results suggested that O.canum was pathogenic to Yunnan pine and that the wide distribution of the fungus might be benefi cial to reproduction of pine shoot beetles in Yunnan pine.However,because the reaction zone and monoterpene accumulation were mild,fungal damage of Yunnan pine might be limited.A more integrated study considering all the fungal species should be done to better understand the interactions among bark beetles,blue-stain fungi,and the tree hosts in the region.
