Greenbug(Schizaphis graminum, Rondani) is a serious insect pest in many wheat growing regions and has been infesting cereal crops in the USA for over a century. Continuous occurrence of new greenbug biotypes makes it ...Greenbug(Schizaphis graminum, Rondani) is a serious insect pest in many wheat growing regions and has been infesting cereal crops in the USA for over a century. Continuous occurrence of new greenbug biotypes makes it essential to explore all greenbug resistant sources available to manage this pest. Gb1, a recessive greenbug resistance gene in DS28A, confers resistance to several economically important greenbug biotypes and is the only gene found to be resistant to greenbug biotype F. A set of 174 F_(2:3)lines from the cross DS28A × Custer was evaluated for resistance to greenbug biotype F in 2020 and 2022. Selective genotyping of the corresponding F_(2) population using single nucleotide polymorphism(SNP) markers generated by genotyping-by-sequencing(GBS) led to the identification of a candidate genomic region for Gb1. Thus, SSR markers previously mapped in this region were used to genotype the entire F2population,and kompetitive allele specific PCR(KASP) markers were also developed from SNPs in the target region.Gb1 was placed in the terminal region of the short arm of chromosome 1A, and its location was confirmed in a second population derived from the cross DS28A × PI 697274. The combined data analysis from the two mapping populations delimited Gb1 to a < 1 Mb interval between 13,328,200 and 14,241,426 bp on1AS.展开更多
为深入了解武夷茶树(Camelliasinensis,异名:TheaboheaL.)的遗传多样性与背景关系,收集126个武夷茶树品种/品系与223个来自12个不同地区的优异茶树品种/品系,共349份茶树资源。采用基因分型测序(Genotyping by sequencing,GBS)技术筛选...为深入了解武夷茶树(Camelliasinensis,异名:TheaboheaL.)的遗传多样性与背景关系,收集126个武夷茶树品种/品系与223个来自12个不同地区的优异茶树品种/品系,共349份茶树资源。采用基因分型测序(Genotyping by sequencing,GBS)技术筛选出973个高质量核心SNP进行茶树遗传多样性及背景关系分析。基于模型的遗传结构(Structure)、系统发育树(NJtree)和主成分分析(PCA)结果表明,349个茶树可分为5个亚群,亚群聚类主要是基于茶树之间的亲缘关系,而不是树型或叶形等形态特征。基因流分析表明,从闽南地区到武夷山地区和武夷山地区到浙江地区存在基因渗入。遗传相似度分析显示,在349个茶树中有136对样本的遗传相似系数大于0.9,其中有26对涉及武夷茶树品种/品系。通过两两比对的辨识度分析,从973个SNP标记中筛选出21个可100%识别349个茶树品种/品系的SNP标记,其中18个SNP标记即可100%识别126个武夷茶树品种/品系,并建立遗传指纹图谱与开发鉴定引物。研究结果为今后武夷茶树种质资源的管理和育种提供有价值的信息。展开更多
目的探讨实时荧光定量聚合酶链式反应(polymerase chain reaction,PCR)技术对检测妊娠晚期B族链球菌(group B streptococcus,GBS)感染的效果。方法选取2021年1—12月江苏省丹阳市妇幼保健院接收的180例妊娠晚期孕妇,均进行实时荧光定量...目的探讨实时荧光定量聚合酶链式反应(polymerase chain reaction,PCR)技术对检测妊娠晚期B族链球菌(group B streptococcus,GBS)感染的效果。方法选取2021年1—12月江苏省丹阳市妇幼保健院接收的180例妊娠晚期孕妇,均进行实时荧光定量PCR检测,以基因测序法为金标准,分析该技术对GBS感染的诊断效能,并根据金标准将患者分为阳性组、阴性组,比较两组不良结局情况。结果实时荧光定量PCR技术检查诊断孕晚期GBS感染的准确率为99.44%、灵敏度为92.86%、特异度为100.00%,与金标准比较一致性较好(Kappa=0.96)。阳性组早产、胎膜早破、宫内感染、胎儿窘迫发生率分别为14.29%、14.29%、21.43%、21.43%,均高于阴性组,差异有统计学意义(χ^(2)=12.741、12.741、24.281、24.281,P<0.05)。阳性组新生儿感染、低体重儿发生率分别为14.29%、21.43%,均高于阴性组,差异有统计学意义(χ^(2)=12.741、24.281,P<0.05)。结论对妊娠晚期GBS感染采用实时荧光定量PCR技术检查诊断效能高,有利于预测不良妊娠结局,可指导临床诊疗工作的开展。展开更多
基金supported by the USDA ARS CRIS Project (3072– 21000–009–00D)。
文摘Greenbug(Schizaphis graminum, Rondani) is a serious insect pest in many wheat growing regions and has been infesting cereal crops in the USA for over a century. Continuous occurrence of new greenbug biotypes makes it essential to explore all greenbug resistant sources available to manage this pest. Gb1, a recessive greenbug resistance gene in DS28A, confers resistance to several economically important greenbug biotypes and is the only gene found to be resistant to greenbug biotype F. A set of 174 F_(2:3)lines from the cross DS28A × Custer was evaluated for resistance to greenbug biotype F in 2020 and 2022. Selective genotyping of the corresponding F_(2) population using single nucleotide polymorphism(SNP) markers generated by genotyping-by-sequencing(GBS) led to the identification of a candidate genomic region for Gb1. Thus, SSR markers previously mapped in this region were used to genotype the entire F2population,and kompetitive allele specific PCR(KASP) markers were also developed from SNPs in the target region.Gb1 was placed in the terminal region of the short arm of chromosome 1A, and its location was confirmed in a second population derived from the cross DS28A × PI 697274. The combined data analysis from the two mapping populations delimited Gb1 to a < 1 Mb interval between 13,328,200 and 14,241,426 bp on1AS.
文摘为深入了解武夷茶树(Camelliasinensis,异名:TheaboheaL.)的遗传多样性与背景关系,收集126个武夷茶树品种/品系与223个来自12个不同地区的优异茶树品种/品系,共349份茶树资源。采用基因分型测序(Genotyping by sequencing,GBS)技术筛选出973个高质量核心SNP进行茶树遗传多样性及背景关系分析。基于模型的遗传结构(Structure)、系统发育树(NJtree)和主成分分析(PCA)结果表明,349个茶树可分为5个亚群,亚群聚类主要是基于茶树之间的亲缘关系,而不是树型或叶形等形态特征。基因流分析表明,从闽南地区到武夷山地区和武夷山地区到浙江地区存在基因渗入。遗传相似度分析显示,在349个茶树中有136对样本的遗传相似系数大于0.9,其中有26对涉及武夷茶树品种/品系。通过两两比对的辨识度分析,从973个SNP标记中筛选出21个可100%识别349个茶树品种/品系的SNP标记,其中18个SNP标记即可100%识别126个武夷茶树品种/品系,并建立遗传指纹图谱与开发鉴定引物。研究结果为今后武夷茶树种质资源的管理和育种提供有价值的信息。
文摘目的探讨实时荧光定量聚合酶链式反应(polymerase chain reaction,PCR)技术对检测妊娠晚期B族链球菌(group B streptococcus,GBS)感染的效果。方法选取2021年1—12月江苏省丹阳市妇幼保健院接收的180例妊娠晚期孕妇,均进行实时荧光定量PCR检测,以基因测序法为金标准,分析该技术对GBS感染的诊断效能,并根据金标准将患者分为阳性组、阴性组,比较两组不良结局情况。结果实时荧光定量PCR技术检查诊断孕晚期GBS感染的准确率为99.44%、灵敏度为92.86%、特异度为100.00%,与金标准比较一致性较好(Kappa=0.96)。阳性组早产、胎膜早破、宫内感染、胎儿窘迫发生率分别为14.29%、14.29%、21.43%、21.43%,均高于阴性组,差异有统计学意义(χ^(2)=12.741、12.741、24.281、24.281,P<0.05)。阳性组新生儿感染、低体重儿发生率分别为14.29%、21.43%,均高于阴性组,差异有统计学意义(χ^(2)=12.741、24.281,P<0.05)。结论对妊娠晚期GBS感染采用实时荧光定量PCR技术检查诊断效能高,有利于预测不良妊娠结局,可指导临床诊疗工作的开展。
