Objective The aim of this study was to investigate macular perfusion changes and ganglion cell complex(GCC)loss in patients with unexplained visual loss following vitrectomy and silicone oil(SO)tamponade,and to evalua...Objective The aim of this study was to investigate macular perfusion changes and ganglion cell complex(GCC)loss in patients with unexplained visual loss following vitrectomy and silicone oil(SO)tamponade,and to evaluate the correlation between retinal blood flow and GCC loss using optical coherence tomography angiography(OCTA)and optical coherence tomography(OCT).Methods This retrospective study included seven eyes(seven patients)with unexpected visual loss after vitrectomy and SO tamponade.OCTA was used to evaluate the alterations in retinal vessel density(VD)in the superficial capillary plexus(SCP),deep capillary plexus(DCP),and radial peripapillary capillary plexus(RPCP).OCT was used to measure the thickness of GCC and retinal nerve fiber layer(RNFL).Medical records of patients were reviewed.Results Quantitative analysis of OCTA images revealed a significant reduction in SCP VD in the affected eyes compared with the controls(all sections P<0.05).No difference was found in GCC thickness,but FLV(focal loss volume)and GLV(global loss volume)were significantly higher in the affected eyes(both P<0.001).SCP VD was inversely correlated with FLV and GLV.Conclusions Silicone oil-related severe visual loss was associated with superficial retinal microvasculature damage and ganglion cell apoptosis.展开更多
AIM： To evaluate the thickness of the retinal layers in the macula using spectral-domain optical coherence tomography（SD-OCT） in patients with neuromyelitis optica（NMO）. METHODS： Spectralis SD-OCT, utilizing aut...AIM： To evaluate the thickness of the retinal layers in the macula using spectral-domain optical coherence tomography（SD-OCT） in patients with neuromyelitis optica（NMO）. METHODS： Spectralis SD-OCT, utilizing automated macular layer segmentation, was performed in 26 NMO patients and 26 healthy controls. Visual function including visual field tests and pattern visual evoked potential were recorded in study subjects.RESULTS： Forty-one eyes from 26 NMO patients and52 eyes from 26 age- and sex-matched healthy controls were included. Besides total macular volume, peri-paipillary retinal nerve fiber layer（RNFL） thickness, the thickness of macular RNFL, ganglion cell layer（GCL） and inner plexiform layer（IPL） were also significantly reduced in NMO patients compared to those inhealthy controls（P 〈0.000）. No differences were found in the thickness of macular inner nuclear layer（INL）, outer plexiform layer（OPL）, and outer nuclear layer（ONL） between the two groups. Reversely, the outer retinal layer（ORL） was shown to be thicker in NMO than controls（P 〈0.05）.Compared with the peri-papillary RNFL thickness, the GCL thickness was demonstrated to correlate with visual function better.CONCLUSION： The study provides in vivo evidence of retinal neural loss in NMO patients and demonstrates abetter structure-function correlation between retinal ganglion cell and visual function than peri-papillary RNFL does. In addition, no evidence of primary neural damage is found. Besides, the photoreceptor cells and retinal pigments epithelial（RPE） cells presumably proliferated in compensation in NMO after retinal neural loss.展开更多
目的探讨不同程度的噪声性听力损伤(NIHL)与大鼠耳蜗螺旋神经节细胞(SGC)Caspase-3表达水平的关系,分析SGC噪声损伤的凋亡机制。方法 SPF级雄性SD大鼠30只随机分为3组,每组10只。除对照组外,其余2组分别予以90、110 d B SPL的白噪声暴露...目的探讨不同程度的噪声性听力损伤(NIHL)与大鼠耳蜗螺旋神经节细胞(SGC)Caspase-3表达水平的关系,分析SGC噪声损伤的凋亡机制。方法 SPF级雄性SD大鼠30只随机分为3组,每组10只。除对照组外,其余2组分别予以90、110 d B SPL的白噪声暴露,每天6 h,连续28 d,暴露前1 d和暴露结束后第7 d进行听性脑干反应(ABR)检测和SGC病理学检查,免疫组化法分析SGC的Caspase-3表达水平。结果各组间大鼠平均永久性听阈位移(PTS)水平变化差异有统计学意义(F=1 103.21,P<0.01),随噪声暴露强度的增加,PTS水平也增加;各组大鼠SGC的Caspase-3平均灰度值(IA)差异有统计学意义(F=1 190.93,P<0.01),随噪声暴露强度的增加,IA值亦增加;病理学观察发现各噪声暴露组均出现SGC的损伤,110 d B SPL噪声暴露组出现明显的SGC溶解现象。结论轻度NIHL即可诱发耳蜗SGC的凋亡。展开更多
基金supported by Capital Medical University Affiliated Beijing Tongren Hospital Key Medical Development Plan[trzdyxzy201801].
文摘Objective The aim of this study was to investigate macular perfusion changes and ganglion cell complex(GCC)loss in patients with unexplained visual loss following vitrectomy and silicone oil(SO)tamponade,and to evaluate the correlation between retinal blood flow and GCC loss using optical coherence tomography angiography(OCTA)and optical coherence tomography(OCT).Methods This retrospective study included seven eyes(seven patients)with unexpected visual loss after vitrectomy and SO tamponade.OCTA was used to evaluate the alterations in retinal vessel density(VD)in the superficial capillary plexus(SCP),deep capillary plexus(DCP),and radial peripapillary capillary plexus(RPCP).OCT was used to measure the thickness of GCC and retinal nerve fiber layer(RNFL).Medical records of patients were reviewed.Results Quantitative analysis of OCTA images revealed a significant reduction in SCP VD in the affected eyes compared with the controls(all sections P<0.05).No difference was found in GCC thickness,but FLV(focal loss volume)and GLV(global loss volume)were significantly higher in the affected eyes(both P<0.001).SCP VD was inversely correlated with FLV and GLV.Conclusions Silicone oil-related severe visual loss was associated with superficial retinal microvasculature damage and ganglion cell apoptosis.
文摘AIM： To evaluate the thickness of the retinal layers in the macula using spectral-domain optical coherence tomography（SD-OCT） in patients with neuromyelitis optica（NMO）. METHODS： Spectralis SD-OCT, utilizing automated macular layer segmentation, was performed in 26 NMO patients and 26 healthy controls. Visual function including visual field tests and pattern visual evoked potential were recorded in study subjects.RESULTS： Forty-one eyes from 26 NMO patients and52 eyes from 26 age- and sex-matched healthy controls were included. Besides total macular volume, peri-paipillary retinal nerve fiber layer（RNFL） thickness, the thickness of macular RNFL, ganglion cell layer（GCL） and inner plexiform layer（IPL） were also significantly reduced in NMO patients compared to those inhealthy controls（P 〈0.000）. No differences were found in the thickness of macular inner nuclear layer（INL）, outer plexiform layer（OPL）, and outer nuclear layer（ONL） between the two groups. Reversely, the outer retinal layer（ORL） was shown to be thicker in NMO than controls（P 〈0.05）.Compared with the peri-papillary RNFL thickness, the GCL thickness was demonstrated to correlate with visual function better.CONCLUSION： The study provides in vivo evidence of retinal neural loss in NMO patients and demonstrates abetter structure-function correlation between retinal ganglion cell and visual function than peri-papillary RNFL does. In addition, no evidence of primary neural damage is found. Besides, the photoreceptor cells and retinal pigments epithelial（RPE） cells presumably proliferated in compensation in NMO after retinal neural loss.
文摘目的探讨不同程度的噪声性听力损伤(NIHL)与大鼠耳蜗螺旋神经节细胞(SGC)Caspase-3表达水平的关系,分析SGC噪声损伤的凋亡机制。方法 SPF级雄性SD大鼠30只随机分为3组,每组10只。除对照组外,其余2组分别予以90、110 d B SPL的白噪声暴露,每天6 h,连续28 d,暴露前1 d和暴露结束后第7 d进行听性脑干反应(ABR)检测和SGC病理学检查,免疫组化法分析SGC的Caspase-3表达水平。结果各组间大鼠平均永久性听阈位移(PTS)水平变化差异有统计学意义(F=1 103.21,P<0.01),随噪声暴露强度的增加,PTS水平也增加;各组大鼠SGC的Caspase-3平均灰度值(IA)差异有统计学意义(F=1 190.93,P<0.01),随噪声暴露强度的增加,IA值亦增加;病理学观察发现各噪声暴露组均出现SGC的损伤,110 d B SPL噪声暴露组出现明显的SGC溶解现象。结论轻度NIHL即可诱发耳蜗SGC的凋亡。
