Objective:To investigate the expression levels of glyceraldehyde-3-phosphate dehydrogenase(GAPDH)and explore its prognostic value across 24 different human cancers.This investigation was conducted using comprehensive ...Objective:To investigate the expression levels of glyceraldehyde-3-phosphate dehydrogenase(GAPDH)and explore its prognostic value across 24 different human cancers.This investigation was conducted using comprehensive bioinformatics and in vitro approaches,incorporating multiple layers of analysis.Methods:GAPDH expression and methylation levels were assessed using bioinformatics tools and validated in cell lines through RNA-seq and targeted bisulfite-seq analyses.The potential prognostic significance of GAPDH was evaluated using the KM plotter.Additionally,cBioPortal was employed to investigate genetic alterations associated with this gene.Pathway analysis was conducted using DAVID.Furthermore,a correlation analysis between GAPDH expression and CD8+T immune cells was performed using TIMER and CDT.Finally,a gene-drug interaction network analysis was conducted using Cytoscape to examine the relationship between GAPDH and various drugs.Results:GAPDH was found to be commonly upregulated in 24 types of human cancers,with its upregulation significantly correlated with poor relapse-free survival(RFS)and overall survival(OS)in BLCA,CESC,HNSC,KIRP,LIHC,and LUAD.This suggests that GAPDH plays a significant role in the development of these cancers.GAPDH upregulation was also associated with various clinicopathological features in patients with BLCA,CESC,HNSC,KIRP,LIHC,and LUAD.Pathway analysis revealed GAPDH’s involvement in diverse pathways.Additionally,notable correlations were observed between GAPDH expression and its promoter methylation level,genetic alterations,and CD8+T immune cell levels.Moreover,several regulatory drugs targeting GAPDH were identified,with the potential to modulate its expression and potentially prevent conditions such as BLCA,CESC,HNSC,KIRP,LIHC,and LUAD.Conclusion:Based on our findings,GAPDH emerges as a promising diagnostic and prognostic biomarker for BLCA,CESC,HNSC,KIRP,LIHC,and LUAD.展开更多
本研究旨在建立鸭GAPDH基因的real-time PCR技术,为鸭功能基因mRNA水平的定量分析提供有用的方法学基础.根据GenBank中鸭GAPDH基因的序列设计引物,建立了基于SYBR Green I染料技术的real-time PCR方法.结果表明,所建立的方法具有快速、...本研究旨在建立鸭GAPDH基因的real-time PCR技术,为鸭功能基因mRNA水平的定量分析提供有用的方法学基础.根据GenBank中鸭GAPDH基因的序列设计引物,建立了基于SYBR Green I染料技术的real-time PCR方法.结果表明,所建立的方法具有快速、高通量、线性范围广以及重复性强等特点.研究结果为鸭GAPDH作为内参基因用于鸭相关基因定量表达分析奠定了基础.展开更多
文摘Objective:To investigate the expression levels of glyceraldehyde-3-phosphate dehydrogenase(GAPDH)and explore its prognostic value across 24 different human cancers.This investigation was conducted using comprehensive bioinformatics and in vitro approaches,incorporating multiple layers of analysis.Methods:GAPDH expression and methylation levels were assessed using bioinformatics tools and validated in cell lines through RNA-seq and targeted bisulfite-seq analyses.The potential prognostic significance of GAPDH was evaluated using the KM plotter.Additionally,cBioPortal was employed to investigate genetic alterations associated with this gene.Pathway analysis was conducted using DAVID.Furthermore,a correlation analysis between GAPDH expression and CD8+T immune cells was performed using TIMER and CDT.Finally,a gene-drug interaction network analysis was conducted using Cytoscape to examine the relationship between GAPDH and various drugs.Results:GAPDH was found to be commonly upregulated in 24 types of human cancers,with its upregulation significantly correlated with poor relapse-free survival(RFS)and overall survival(OS)in BLCA,CESC,HNSC,KIRP,LIHC,and LUAD.This suggests that GAPDH plays a significant role in the development of these cancers.GAPDH upregulation was also associated with various clinicopathological features in patients with BLCA,CESC,HNSC,KIRP,LIHC,and LUAD.Pathway analysis revealed GAPDH’s involvement in diverse pathways.Additionally,notable correlations were observed between GAPDH expression and its promoter methylation level,genetic alterations,and CD8+T immune cell levels.Moreover,several regulatory drugs targeting GAPDH were identified,with the potential to modulate its expression and potentially prevent conditions such as BLCA,CESC,HNSC,KIRP,LIHC,and LUAD.Conclusion:Based on our findings,GAPDH emerges as a promising diagnostic and prognostic biomarker for BLCA,CESC,HNSC,KIRP,LIHC,and LUAD.
文摘本研究旨在建立鸭GAPDH基因的real-time PCR技术,为鸭功能基因mRNA水平的定量分析提供有用的方法学基础.根据GenBank中鸭GAPDH基因的序列设计引物,建立了基于SYBR Green I染料技术的real-time PCR方法.结果表明,所建立的方法具有快速、高通量、线性范围广以及重复性强等特点.研究结果为鸭GAPDH作为内参基因用于鸭相关基因定量表达分析奠定了基础.
