[Objective] This research aimed at constructing two gateway-compatible plant expression vectors for functional genomics of abiotic stress in tomato.[Method] pK2GW7I was generated from the plant expression vector pK2GW...[Objective] This research aimed at constructing two gateway-compatible plant expression vectors for functional genomics of abiotic stress in tomato.[Method] pK2GW7I was generated from the plant expression vector pK2GW7,0 by replacing the CaMV 35S promoter with abiotic-stress inducible plant promoter pRD29A,which was derived from the promoter of Arabidopsis gene RD29A.pKGW121 was generated by replacing the CaMV 35S of the plant expression vector pRD410 with the gateway recombinant cassette(attR1-cmR-ccdB-attR2)from pK2GW7,0.Constitutive and root-specific promoters were tested on pKGW121.[Result] Two gateway-compatible destination vectors,pK2GW7I and pKGW121,were successfully constructed;practicability test proved that pKGW121 was applicable for promoter analysis.[Conclusion] With the incorporation of gateway cloning technology or abiotic-stress inducible promoter,pKGW121 and pK2GW7I will be promising in large-scale investigation of tomato functional genes associated with various abiotic stresses.A high-throughput workflow for the construction of plant transformation vector was proposed and validated.展开更多
传统的基于专用短程通信(dedicated short range communication,DSRC)的车载网络(vehicular ad hoc network,VANET)通信架构难以满足车联网数据传输的服务质量(quality of service,QoS)需求,通过移动网关将数据上传至服务器,由服务器决...传统的基于专用短程通信(dedicated short range communication,DSRC)的车载网络(vehicular ad hoc network,VANET)通信架构难以满足车联网数据传输的服务质量(quality of service,QoS)需求,通过移动网关将数据上传至服务器,由服务器决策传输给目标车辆,可以扩大数据广播域,极大减少数据远程传输时延.结合移动云服务的思想,提出了一种新的车联网架构和数据传输方法.首先给出了网关服务者(gateway server,GWS)向云端注册服务信息的具体流程;其次提出了一种云端服务网关选取方法,该方法结合云端的历史数据和实时数据,动态决定参与服务的网关服务者及其服务范围,网关消费者(gateway consumer,GWC)在获取服务广播消息后,综合考虑通信负载、链路稳定度、信道质量等性能参数来选出最优的网关服务者,并将数据传输给网关服务者,再由其上传到云端;最后在OMNeT++实验环境下,针对不同的交通场景,对该方法传输性能进行了评估.结果表明该方法获得较低传输延迟的同时,能够保证较高的传输成功率,理论分析也证明了该方法的有效性.展开更多
Based on the analysis of developing target, function and constitution of cloth order system under wap gateway, the mobile communication principle of cloth order system under wap gateway is given out. The implementatio...Based on the analysis of developing target, function and constitution of cloth order system under wap gateway, the mobile communication principle of cloth order system under wap gateway is given out. The implementation method for information communication of cloth order system are elaborated by using programming technology of active server page (ASP)3.0 and wireless markup language (WML). The browsing and inquiry of cloth information, sending and receiving of cloth order can be easily realized by the developed order system on mobile internet according to user’s requirements.展开更多
基金Supported by the Natural Science Foundation of China(30771461)the Wuhan Chenguang Program of Hubei Provicne of China(20055003059-24)
文摘[Objective] This research aimed at constructing two gateway-compatible plant expression vectors for functional genomics of abiotic stress in tomato.[Method] pK2GW7I was generated from the plant expression vector pK2GW7,0 by replacing the CaMV 35S promoter with abiotic-stress inducible plant promoter pRD29A,which was derived from the promoter of Arabidopsis gene RD29A.pKGW121 was generated by replacing the CaMV 35S of the plant expression vector pRD410 with the gateway recombinant cassette(attR1-cmR-ccdB-attR2)from pK2GW7,0.Constitutive and root-specific promoters were tested on pKGW121.[Result] Two gateway-compatible destination vectors,pK2GW7I and pKGW121,were successfully constructed;practicability test proved that pKGW121 was applicable for promoter analysis.[Conclusion] With the incorporation of gateway cloning technology or abiotic-stress inducible promoter,pKGW121 and pK2GW7I will be promising in large-scale investigation of tomato functional genes associated with various abiotic stresses.A high-throughput workflow for the construction of plant transformation vector was proposed and validated.
文摘传统的基于专用短程通信(dedicated short range communication,DSRC)的车载网络(vehicular ad hoc network,VANET)通信架构难以满足车联网数据传输的服务质量(quality of service,QoS)需求,通过移动网关将数据上传至服务器,由服务器决策传输给目标车辆,可以扩大数据广播域,极大减少数据远程传输时延.结合移动云服务的思想,提出了一种新的车联网架构和数据传输方法.首先给出了网关服务者(gateway server,GWS)向云端注册服务信息的具体流程;其次提出了一种云端服务网关选取方法,该方法结合云端的历史数据和实时数据,动态决定参与服务的网关服务者及其服务范围,网关消费者(gateway consumer,GWC)在获取服务广播消息后,综合考虑通信负载、链路稳定度、信道质量等性能参数来选出最优的网关服务者,并将数据传输给网关服务者,再由其上传到云端;最后在OMNeT++实验环境下,针对不同的交通场景,对该方法传输性能进行了评估.结果表明该方法获得较低传输延迟的同时,能够保证较高的传输成功率,理论分析也证明了该方法的有效性.
文摘Based on the analysis of developing target, function and constitution of cloth order system under wap gateway, the mobile communication principle of cloth order system under wap gateway is given out. The implementation method for information communication of cloth order system are elaborated by using programming technology of active server page (ASP)3.0 and wireless markup language (WML). The browsing and inquiry of cloth information, sending and receiving of cloth order can be easily realized by the developed order system on mobile internet according to user’s requirements.
