Objective:This study aims to explore the metabolic mechanism of the liver protective effect of an aqueous extract of Gei Herba(AEG)in blood deficiency(BD)mice.Methods:The BD mouse model was established by acetylphenyl...Objective:This study aims to explore the metabolic mechanism of the liver protective effect of an aqueous extract of Gei Herba(AEG)in blood deficiency(BD)mice.Methods:The BD mouse model was established by acetylphenylhydrazine and cyclophosphamide.A total of forty-eight female Kunming mice(18–22 g)were randomly assigned to six groups:a control group,a BD model group,a positive drug group(Danggui Yixue oral liquid),and three AEG treatment groups receiving a daily AEG dose of 1,2,4 g/kg for nine days.The serum levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST),and the protein expression of IL-1,IL-2,IL-3 and TNF-αwere determined by enzyme-linked immunosorbent assay.The liquid chromatography-mass spectrometry(LC-MS)based metabolomics was used to identify liver metabolites,and the MetaboAnalyst platform was used to analyze the metabolic pathways.Results:AEG reduced serum AST,increased IL-1,IL-2,IL-3 and decreased TNF-αto effectively alleviate liver damage in BD mice.Furthermore,metabolomics analysis revealed that the disordered mice liver metabolic profile was corrected after AEG treatment,five differential metabolites were upregulated,and the mechanism was mainly related to increasing the primary bile acid biosynthesis.Conclusion:AEG has a regulatory effect on abnormal liver metabolism in BD mice.展开更多
GEIS(Grid Based Emergency Inter-Act System)是一个基于主体网格的城市应急联动与社会综合服务系统,通过主体网格和通信系统将治安、消防、卫生急救、交通、公共设施、自然灾害等突发事件的应急联动与综合服务,调度集成在一个管理平台...GEIS(Grid Based Emergency Inter-Act System)是一个基于主体网格的城市应急联动与社会综合服务系统,通过主体网格和通信系统将治安、消防、卫生急救、交通、公共设施、自然灾害等突发事件的应急联动与综合服务,调度集成在一个管理平台中,通过共享指挥平台和基础信息,实现统一接处警,统一指挥、联合行动、快速反应、为市民提供更加便捷的紧急救援及相关服务,为政府科学决策和处置各种紧急与灾害事件提供技术支持,为城市公共安全提供技术保障。GEIS的一个突出优势是将范例推理(Case Base Reasoning,CBR)与规则推理(Rule Base Reasoning,RBR)两种人工智能技术相结合,实现了快速预案生成系统。它能在紧急情况发生的时候,为应急部门提供快速的辅助预案支持,为科学决策打下良好基础。目前,GEIS已经应用到抗洪抢险和交通事故处理中,效果表明它具有跨平台性好,稳定性强,响应速度快,易操作的独特优势。展开更多
Background:To explore active components of Lanbuzheng(Gei herba)and its underlying complex mechanism in treating blood deficiency induced by chemotherapy drug based on network pharmacology and mice experimental valida...Background:To explore active components of Lanbuzheng(Gei herba)and its underlying complex mechanism in treating blood deficiency induced by chemotherapy drug based on network pharmacology and mice experimental validation.Methods:Active components of Lanbuzheng(Gei herba)were screened by Lipinski’s rule of five.Targets acted with active components were predicted by PharmMapper database,and targets whose function associated with blood deficiency were screened by Therapeutic Target Database and UniProt.The networks of component-target and target-pathway were constructed by Cytoscape.The levels of peripheral blood and organ indexes were detected in the animal experiments.Results:One hundred and seventy-three components of Lanbuzheng(Gei herba)were collected,and 60 active components were screened according to the rule of five.According to the degree value of compounds,the top 5 compounds were docosyl trans ferulate,C32 decursin,agrimonolide 6-O-β-D-glucoside,degree=11,173-ethoxyphaeophorbide,and eugenol.Finally,59 targets associated with blood deficiency were obtained and the top 5 targets were MAPK14,TTR,CDK2,AKR1B1 and AR.Based on the interaction network of componenttarget and target-pathway,it’s found that 60 active components could act with 59 targets and 44 pathways for treating blood deficiency.And then,the mice experiments showed that Lanbuzheng(Gei herba)could enrich blood by increasing the levels of red blood cell,white blood cell,hemoglobin,red blood cell specific volume and platelet,and the indexes of liver,thymus and spleen,which validated the treating effect of Lanbuzheng(Gei herba).Conclusion:In this study,a network pharmacology approach and animal experiments were established to explore the nourishing blood effect of Lanbuzheng(Gei herba).The results demonstrated that Lanbuzheng(Gei herba)could improve blood deficiency and provide a theoretical basis for the further research on the in-depth mechanism of Lanbuzheng(Gei herba).展开更多
Global Energy Interconnection(GEI)is a bold venture for the generation,transmission,and use of clean energy,applying a smart grid as well as ultra-high voltage transmissions to transport renewable energy over long dis...Global Energy Interconnection(GEI)is a bold venture for the generation,transmission,and use of clean energy,applying a smart grid as well as ultra-high voltage transmissions to transport renewable energy over long distances and with minimal losses.Through its organizational arm,the Global Energy Interconnection Development Cooperation Organization(GEIDCO),GEI pursues展开更多
文摘Objective:This study aims to explore the metabolic mechanism of the liver protective effect of an aqueous extract of Gei Herba(AEG)in blood deficiency(BD)mice.Methods:The BD mouse model was established by acetylphenylhydrazine and cyclophosphamide.A total of forty-eight female Kunming mice(18–22 g)were randomly assigned to six groups:a control group,a BD model group,a positive drug group(Danggui Yixue oral liquid),and three AEG treatment groups receiving a daily AEG dose of 1,2,4 g/kg for nine days.The serum levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST),and the protein expression of IL-1,IL-2,IL-3 and TNF-αwere determined by enzyme-linked immunosorbent assay.The liquid chromatography-mass spectrometry(LC-MS)based metabolomics was used to identify liver metabolites,and the MetaboAnalyst platform was used to analyze the metabolic pathways.Results:AEG reduced serum AST,increased IL-1,IL-2,IL-3 and decreased TNF-αto effectively alleviate liver damage in BD mice.Furthermore,metabolomics analysis revealed that the disordered mice liver metabolic profile was corrected after AEG treatment,five differential metabolites were upregulated,and the mechanism was mainly related to increasing the primary bile acid biosynthesis.Conclusion:AEG has a regulatory effect on abnormal liver metabolism in BD mice.
文摘GEIS(Grid Based Emergency Inter-Act System)是一个基于主体网格的城市应急联动与社会综合服务系统,通过主体网格和通信系统将治安、消防、卫生急救、交通、公共设施、自然灾害等突发事件的应急联动与综合服务,调度集成在一个管理平台中,通过共享指挥平台和基础信息,实现统一接处警,统一指挥、联合行动、快速反应、为市民提供更加便捷的紧急救援及相关服务,为政府科学决策和处置各种紧急与灾害事件提供技术支持,为城市公共安全提供技术保障。GEIS的一个突出优势是将范例推理(Case Base Reasoning,CBR)与规则推理(Rule Base Reasoning,RBR)两种人工智能技术相结合,实现了快速预案生成系统。它能在紧急情况发生的时候,为应急部门提供快速的辅助预案支持,为科学决策打下良好基础。目前,GEIS已经应用到抗洪抢险和交通事故处理中,效果表明它具有跨平台性好,稳定性强,响应速度快,易操作的独特优势。
基金This study was supported by the National Natural Science Foundation of China(No.81760746 and 81803838)Education Department of Guizhou Province of China(GNYL[2017]006)+3 种基金Provincial Department of Education youth talent support program(qiankehe KY[2017]078)Key project at central government level:The ability establishment of sustainable use for valuable Chinese medicine resources(2060302)Science and Technology Department of Zunyi City of Zuniyi City of Guizhou Province of China([2016]35,[2016]33)Innovation talent team of Guizhou science and Technology Department(qiankehe platform talents[2020]5007).
文摘Background:To explore active components of Lanbuzheng(Gei herba)and its underlying complex mechanism in treating blood deficiency induced by chemotherapy drug based on network pharmacology and mice experimental validation.Methods:Active components of Lanbuzheng(Gei herba)were screened by Lipinski’s rule of five.Targets acted with active components were predicted by PharmMapper database,and targets whose function associated with blood deficiency were screened by Therapeutic Target Database and UniProt.The networks of component-target and target-pathway were constructed by Cytoscape.The levels of peripheral blood and organ indexes were detected in the animal experiments.Results:One hundred and seventy-three components of Lanbuzheng(Gei herba)were collected,and 60 active components were screened according to the rule of five.According to the degree value of compounds,the top 5 compounds were docosyl trans ferulate,C32 decursin,agrimonolide 6-O-β-D-glucoside,degree=11,173-ethoxyphaeophorbide,and eugenol.Finally,59 targets associated with blood deficiency were obtained and the top 5 targets were MAPK14,TTR,CDK2,AKR1B1 and AR.Based on the interaction network of componenttarget and target-pathway,it’s found that 60 active components could act with 59 targets and 44 pathways for treating blood deficiency.And then,the mice experiments showed that Lanbuzheng(Gei herba)could enrich blood by increasing the levels of red blood cell,white blood cell,hemoglobin,red blood cell specific volume and platelet,and the indexes of liver,thymus and spleen,which validated the treating effect of Lanbuzheng(Gei herba).Conclusion:In this study,a network pharmacology approach and animal experiments were established to explore the nourishing blood effect of Lanbuzheng(Gei herba).The results demonstrated that Lanbuzheng(Gei herba)could improve blood deficiency and provide a theoretical basis for the further research on the in-depth mechanism of Lanbuzheng(Gei herba).
文摘Global Energy Interconnection(GEI)is a bold venture for the generation,transmission,and use of clean energy,applying a smart grid as well as ultra-high voltage transmissions to transport renewable energy over long distances and with minimal losses.Through its organizational arm,the Global Energy Interconnection Development Cooperation Organization(GEIDCO),GEI pursues
