Aim A novel method has been developed for evaluation of the levels of total residual protein in antibiotics produced by fermentation using gel filtration chromatography (GFC) combined with Bradford assay based on dete...Aim A novel method has been developed for evaluation of the levels of total residual protein in antibiotics produced by fermentation using gel filtration chromatography (GFC) combined with Bradford assay based on determination of residual protein in lincomycin hydrochloride. Methods The chromatographic conditions were SuperdexTM peptide column, 0.01 mol*L-1 phosphate buffer solution as mobile phase, and flow rate of 1 mL·min-1. Five hundred microliters of lincomycin hydrochloride solution (3 g of lincomycin hydrochloride dissolved in 10 mL of mobile phase) was injected into the chromatograph and the eluted solution was collected between 6 min and 14.5 min (protein eluted from column within this period), and the residual content of total protein in the eluted solution was assayed using Bradford assay method. Results The average recovery was more than 90% for bovine serum albumin, the calibration equation for the range of 0-12 μg·mL-1 of protein was y=-0.002 4x2+0.064 2x+0.002 9, r2=0.999 9, RSD=0.1%-0.9%, and the LOD and LOQ were 3 and 10 ng·mL-1 of protein, respectively. Conclusion The novel method for determining the residual protein in ferment antibio-tics is simple, rapid, and precise.展开更多
In order to recover the SiO_2 contained in the mother liquor in the course ofNaY zeolite synthesis to minimize pollution, the influence of various preparation conditions on thefiltering velocity of gel slurry was stud...In order to recover the SiO_2 contained in the mother liquor in the course ofNaY zeolite synthesis to minimize pollution, the influence of various preparation conditions on thefiltering velocity of gel slurry was studied using the SiO_2/Al_2O_3 gel recovered from the NaYmother liquor in the laboratory. The results of study had shown that at a SiO_2/Al_2O_3 ratio in thefeed equating to 9∶1 the SiO_2 recovery rate and Al_2O_3 utilization rate were high with a fasterflow velocity of the filtrate. The pH value of the system had great impact on the flow velocity offiltrate. Between the two methods for regulating the pH value, the one for formation ofsilica/alumina gel slurry by addition of sulfuric acid prior to adding aluminium sulfate in thesolution could secure a faster filtration velocity. The filtration velocity was decreased in tandemwith increasing SiO_2 concentration in mother liquor, meanwhile an increase in dry filter cakeyield.展开更多
文摘Aim A novel method has been developed for evaluation of the levels of total residual protein in antibiotics produced by fermentation using gel filtration chromatography (GFC) combined with Bradford assay based on determination of residual protein in lincomycin hydrochloride. Methods The chromatographic conditions were SuperdexTM peptide column, 0.01 mol*L-1 phosphate buffer solution as mobile phase, and flow rate of 1 mL·min-1. Five hundred microliters of lincomycin hydrochloride solution (3 g of lincomycin hydrochloride dissolved in 10 mL of mobile phase) was injected into the chromatograph and the eluted solution was collected between 6 min and 14.5 min (protein eluted from column within this period), and the residual content of total protein in the eluted solution was assayed using Bradford assay method. Results The average recovery was more than 90% for bovine serum albumin, the calibration equation for the range of 0-12 μg·mL-1 of protein was y=-0.002 4x2+0.064 2x+0.002 9, r2=0.999 9, RSD=0.1%-0.9%, and the LOD and LOQ were 3 and 10 ng·mL-1 of protein, respectively. Conclusion The novel method for determining the residual protein in ferment antibio-tics is simple, rapid, and precise.
文摘In order to recover the SiO_2 contained in the mother liquor in the course ofNaY zeolite synthesis to minimize pollution, the influence of various preparation conditions on thefiltering velocity of gel slurry was studied using the SiO_2/Al_2O_3 gel recovered from the NaYmother liquor in the laboratory. The results of study had shown that at a SiO_2/Al_2O_3 ratio in thefeed equating to 9∶1 the SiO_2 recovery rate and Al_2O_3 utilization rate were high with a fasterflow velocity of the filtrate. The pH value of the system had great impact on the flow velocity offiltrate. Between the two methods for regulating the pH value, the one for formation ofsilica/alumina gel slurry by addition of sulfuric acid prior to adding aluminium sulfate in thesolution could secure a faster filtration velocity. The filtration velocity was decreased in tandemwith increasing SiO_2 concentration in mother liquor, meanwhile an increase in dry filter cakeyield.
