Background:Microfluidic systems have advantages such as a high throughput,small reaction volume,and precise control of the cellular position and environment.These advantages have allowed microfluidics to be widely use...Background:Microfluidic systems have advantages such as a high throughput,small reaction volume,and precise control of the cellular position and environment.These advantages have allowed microfluidics to be widely used in several fields of synthetic biology in recent years.Results'.In this article,we reviewed the microfluidic-based methods for synthetic biology from two aspects:the construction of synthetic gene circuits and the analysis of synthetic gene systems.We used some examples to illuminate the progresses and challenges in the steps of synthetic gene circuits construction and approaches of gene expression analysis with microfluidic systems.Conclusion:Comparing to traditional methods,microfluidic tools promise great advantages in the synthetic genetic circuit building and analysis process.Moreover,new microfluidic systems together with the mathematical modeling of synthetic circuits or consortiums are desirable to perform complex genetic circuit construction and understand the natural gene regulation in cells and population interactions better.展开更多
基金This study was supported the National Key Research and Development Project(SQ2018YFA090070-03 and 2020YFA0906900)the National Natural Science Foundation of China(Nos.11974002 and 11674010).
文摘Background:Microfluidic systems have advantages such as a high throughput,small reaction volume,and precise control of the cellular position and environment.These advantages have allowed microfluidics to be widely used in several fields of synthetic biology in recent years.Results'.In this article,we reviewed the microfluidic-based methods for synthetic biology from two aspects:the construction of synthetic gene circuits and the analysis of synthetic gene systems.We used some examples to illuminate the progresses and challenges in the steps of synthetic gene circuits construction and approaches of gene expression analysis with microfluidic systems.Conclusion:Comparing to traditional methods,microfluidic tools promise great advantages in the synthetic genetic circuit building and analysis process.Moreover,new microfluidic systems together with the mathematical modeling of synthetic circuits or consortiums are desirable to perform complex genetic circuit construction and understand the natural gene regulation in cells and population interactions better.
基金国家重点基础研究发展规划(973)(the National Grand Fundamental Research 973 Program of China under Grant No.2004CB318103)国家自然科学基金(the National Natural Science Foundation of China under Grant No.60133010)江西省研究生创新基金项目(No YC07A073)
