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Identification of a gene engineering antibody against cystic echinococcosis in liver
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作者 Xin-Hua Chen Hao Wen +3 位作者 Yao-Xin Zhang Xiao-Hui Feng Xiao-Mei Lu Dong Ma the Xinjiang Hydatid Clinical Research Institute and the Department of Infectious Diseases First Teaching Hospital, Xinjiang Medical University, Urumqi 830054, China 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2003年第3期383-386,共4页
OBJECTIVE: To identify a gene engineering antibody against cystic echinococcosis in liver. METHODS: A single chain of variable fragment of human antibodies (ScFvs) was selected from the library by using affinity selec... OBJECTIVE: To identify a gene engineering antibody against cystic echinococcosis in liver. METHODS: A single chain of variable fragment of human antibodies (ScFvs) was selected from the library by using affinity selection technique with the recombinant antigen on solid surface. The positive clones were demonstrated by ELISA and their DNA sequences were also determined. RESULTS: The DNA sequence data showed that the antibody gene is composed of 768bp. In addition, a specific combination capacity with recombinant Echinococcus granulosus antigen B (r-EgB) was demonstrated by ELISA. CONCLUSION: The obtained gene engineering antibody against r-EgB may have potential implications in immunological treatment and drug targeting delivery. 展开更多
关键词 cystic echinococcosis in liver gene engineering antibody phage display single chain of varlable fragment of human antibody recombinant Echinococcus granulosus antigen B
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GENE ENGINEERING EB VIRUS MEMBRANE ANTIGEN IN DETECTION OF MA-IgA ANTIBODY(COMPARISON WITH VCA-IgA AND EA-IgA ANTIBODIES)
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作者 刘孟忠 李振权 皮国华 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1991年第2期33-36,共4页
With gene engineering EB virus membrane antigen as the diagnostic antigen, indirect immunofluo-rescence (IF) assay was used to detect IgA antibody against EB virus membrane antigen (MA-IgA) in sera from 202 nasopharyn... With gene engineering EB virus membrane antigen as the diagnostic antigen, indirect immunofluo-rescence (IF) assay was used to detect IgA antibody against EB virus membrane antigen (MA-IgA) in sera from 202 nasopharyngeal carcinoma (NPC) patients and 315 controls (normal and patients with other tumors). MA-IgA antibody was positive in 96.8% of the pretreatment NPC patients with a GMT of 1:36.3. MA-IgA detection by this method was more sensitive than EA-IgA detection by IE. In contrast, patients with tumors other than NPC were negative for MA-IgA antibody. 9.1% of VCA-IgA positive persons were MA-IgA positive with a GMT of less than 1:5. No MA-IgA positive was found in VCA-IgA negatives. The results indicated that this method was relatively specific. In the treatment group, the positive rate and GMT of MA-IgA antibody declined with increase in survival time and the decline was faster than VCA-IgA. When recurrence or distant metastasis developed, similar to VCA-IgA and EA-IgA antibodies, the positive rate and GMT of MA-IgA antibody increased to its pretreatment level. Therefore, MA-IgA detection might be valuable in the early diagnosis and monitor of NPC. 展开更多
关键词 IgA COMPARISON WITH VCA-IgA AND EA-IgA ANTIBODIES gene ENGINEERING EB VIRUS MEMBRANE ANTIGEN IN DETECTION OF MA-IgA antibody VCA MA EA
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