期刊文献+
共找到2,434篇文章
< 1 2 122 >
每页显示 20 50 100
PCA3 and TMPRSS2-ERG gene fusions as diagnostic biomarkers for prostate cancer 被引量:13
1
作者 Zheng Yang Lu Yu Zhe Wang 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2016年第1期65-71,共7页
The incidence of prostate cancer (PCa) is rising steadily among males in many countries. Serum prostate-specific antigen (PSA) is widely applied to clinical diagnosis and screening of PCa. However, the so-called g... The incidence of prostate cancer (PCa) is rising steadily among males in many countries. Serum prostate-specific antigen (PSA) is widely applied to clinical diagnosis and screening of PCa. However, the so-called grey area of PSA levels 4.0-10.0 ng/mL has a low specificity of 25-40% resulting in a high rate of negative biopsy and overtreatment. So in order to treat PCa patients in early stage, there is an urgent need for new biomarkers in PCa diagnosis. The PCA3 gene, a non-coding RNA (ncRNA) that is highly expressed in prostate cancer (PCa) cells, has been identified as a molecular biomarkers to detect PCa, of which PCA3 has already under clinical application. PCA3 is strongly overexpressed in malignant prostate tissue compared to benign or normal adjacent one. Newly, PCA3 is considered to be a promising biomarker in clinical diagnosis and targeted therapy. The diagnostic significance of PCA3, however, is awaiting further researches. Moreover, it has been demonstrated recently that TMPRSS2-ERG gene fusion is identified as the predominant genetic change in patients diagnosed with PCa. Recent study revealed that combination of the PC43 and TMPRSS2-ERG gene fusion test optimizes PCa detection compared with that of single biomarker, which would lead to a considerable reduction of the number of prostate biopsies. In this review, we focused on the potential use of PCA3 and TMPRSS2-ERG gene fusion detection in the diagnosis of PCa. 展开更多
关键词 Prostate prostate cancer antigen 3 (PCA3) TMPRSS2-ERG gene fusion prostate cancer (PCa) biomarker
下载PDF
Profiling of gene fusion involving targetable genes in Chinese gastric cancer
2
作者 Zhen-Hua Liu Bo-Wen Zhu +11 位作者 Min Shi Yu-Rong Qu Xun-Jun He Hong-Ling Yuan Jie Ma Wei Li Dan-Dan Zhao Zheng-Chuang Liu Bao-Ming Wang Chun-Yang Wang Hou-Quan Tao Tong-Hui Ma 《World Journal of Gastrointestinal Oncology》 SCIE 2022年第8期1528-1539,共12页
BACKGROUND Approximately half of all new cases of gastric cancer(GC)and related deaths occur in China.More than 80%of patients with GC are diagnosed at an advanced stage,which results in poor prognosis.Although HER2-d... BACKGROUND Approximately half of all new cases of gastric cancer(GC)and related deaths occur in China.More than 80%of patients with GC are diagnosed at an advanced stage,which results in poor prognosis.Although HER2-directed therapy and immune checkpoint inhibitors have been somewhat successful,new drugs are still needed for the treatment of GC.Notably,several gene fusion-targeted drugs have been approved by the United States Food and Drug Administration for solid tumors,including GC,such as larotrectinib for NTRK fusion-positive cancers and zenocutuzumab for NRG1 fusion-positive cancers.However,gene fusions involving targetable genes have not been well characterized in Chinese patients with GC.AIM To identify the profile of fusions involving targetable genes in Chinese patients with GC using clinical specimens and determine the distribution of patients with gene fusion variants among the molecular subtypes of GC.METHODS We retrospectively analyzed gene fusion events in tumor tissue samples from 954 Chinese patients with GC.Clinicopathological characteristics were obtained from their medical records.Genetic alterations,such as single nucleotide variants,indels,amplifications,and gene fusions,were identified using a targeted sequencing panel containing 825 genes.Fusions were validated by fluorescence in situ hybridization(FISH)using break-apart probes.The microsatellite instability(MSI)status was evaluated using MSIsensor from the targeted sequencing panel data.Tumor mutational burden(TMB)was calculated using the total number of nonsynonymous mutations divided by the total genomic targeted region.Chi-square analysis was used to determine the enrichment of gene fusions associated with the molecular subtypes of GC.RESULTS We found that 1.68%(16/954)of patients harbored 20 fusion events involving targetable genes.RARA fusions(n=5)were the most common,followed by FGFR2,BRAF,MET,FGFR3,RET,ALK,EGFR,NTRK2,and NRG1 fusions.Two of the RARA fusions,EML4-ALK(E6:E20)and EGFRSEPTIN14(E7:E10),have been identified in other tumors but not in GC.Surprisingly,18 gene fusion events were previously not reported in any cancer types.Twelve of the eighteen novel gene fusions included complete exons encoding functional domains of targetable genes,such as the tyrosine kinase domain of receptor tyrosine kinases and the DNA-and ligand-binding domains of RARA.Consistent with the results of detection using the targeted sequencing fusion panel,the results of FISH(fluorescence in situ hybridization)confirmed the rearrangement of FGFR2 and BRAF in tumors from patients 04 and 09,respectively.Genetic analysis indicated that the fusion genes were significantly enriched in patients with ERBB2 amplification(P=0.02);however,there were no significant differences between fusion-positive and fusion-negative patients in age,sex,MSI status,and TMB.CONCLUSION We characterized the landscape of fusions involving targetable genes in a Chinese GC cohort and found that 1.68%of patients with GC harbor potential targetable gene fusions,which were enriched in patients with ERBB2 amplification.Gene fusion detection may provide a potential treatment strategy for patients with GC with disease progression following standard therapy. 展开更多
关键词 gene fusion Targetable genes Gastric cancer Chinese population ERBB2 amplification
下载PDF
Secretory carcinoma——impact of translocation and gene fusions on salivary gland tumor
3
作者 Ryoko Inaki Masanobu Abe +4 位作者 Liang Zong Takahiro Abe Aya Shinozaki-Ushiku Tetsuo Ushiku Kazuto Hoshi 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2017年第5期379-384,共6页
Secretory carcinoma(SC), previously described as mammary analogue secretory carcinoma(MASC), is a recently described salivary gland tumor which morphologically resembles mammary secretory carcinoma. The first desc... Secretory carcinoma(SC), previously described as mammary analogue secretory carcinoma(MASC), is a recently described salivary gland tumor which morphologically resembles mammary secretory carcinoma. The first description of SC/MASC, reported by Skálová et al. in 2010, was as a rare salivary carcinoma imitating secretory carcinoma of the breast. SC/MASC is a unique salivary gland tumor with morphological overlap with acinic cell carcinoma(Aci CC), mucoepidermoid carcinoma(MEC), and adenocarcinoma not otherwise specified(ADCNOS). SC/MASC shares similar clinicopathological features with Aci CC. As a critical difference between SC/MASC and Aci CC, SC/MASC characteristically has the chromosomal translocation t(12;15)(p13;q25) which leads to a fusion gene between the ETV6 gene on chromosome 12 and the NTRK3 gene on chromosome 15. This genetic background is an important differential diagnostic finding for excluding other salivary gland tumors and may be a critical factor determining the prognosis for patients with SC/MASC. Research in recent years has provided a large body of new data on SC/MASC and suggests the possibility that the ETV6-NTRK3 translocation could be a therapeutic target. Here, we review the morphological and clinicopathological features of SC/MASC and discuss new directions for therapy. 展开更多
关键词 Secretory carcinoma mammary analogue secretory carcinoma MASC ETV6-NTRK3 fusion gene salivary gland tumor
下载PDF
Gene fusions and chimeric RNAs,and their implications in cancer 被引量:4
4
作者 Hao Wu Xiaorong Li Hui Li 《Genes & Diseases》 SCIE 2019年第4期385-390,共6页
Gene fusions are appreciated as ideal cancer biomarkers and therapeutic targets.Chimeric RNAs are traditionally thought to be products of gene fusions,and thus,also cancerspecific.Recent research has demonstrated that... Gene fusions are appreciated as ideal cancer biomarkers and therapeutic targets.Chimeric RNAs are traditionally thought to be products of gene fusions,and thus,also cancerspecific.Recent research has demonstrated that chimeric RNAs can be generated by intergenic splicing in the absence of gene fusion,and such chimeric RNAs are also found in normal physiology.These new findings challenge the traditional theory of chimeric RNAs exclusivity to cancer,and complicates use of chimeric RNAs in cancer detection.Here,we provide an overview of gene fusions and chimeric RNAs,and emphasize their differences.We note that gene fusions are able to generate chimeric RNAs in accordance with the central dogma of biology,and that chimeric RNAs may also be able to influence the generation of the gene fusions per the“horse before the cart”hypothesis.We further expand upon the“horse before the cart”hypothesis,summarizing current evidence in support of the theory and exploring its potential impact on the field. 展开更多
关键词 Chimeric RNA cis-splicing between adjacent genes Chromosomal rearrangement gene fusion Intergenic splicing TRANS-SPLICING
原文传递
Detection of the SYT-SSX Fusion Gene in Synovial Sarcoma 被引量:1
5
作者 杨翎 范钦和 张炜明 《Journal of Nanjing Medical University》 2004年第1期36-39,共4页
Objective: To investigate the feasibility and significance of detecting SYT-SSX fusion gene in paraffin-embedded tissues of synovial sarcoma (SS) by reverse-transcriptase polymerase chain reaction(RT-PCR) methods. Met... Objective: To investigate the feasibility and significance of detecting SYT-SSX fusion gene in paraffin-embedded tissues of synovial sarcoma (SS) by reverse-transcriptase polymerase chain reaction(RT-PCR) methods. Methods: Twenty cases of SS tumors from archival materials were collected and all samples were formalin-fixed and paraffin-embedded (FFPE). SYT-SSX fusion transcript was detected by RT-PCR. Home-keeping gene Porphobilinogen Deaminase (PBGD) was regarded as internal control.Results: PBGD mRNA was detected in all 20 tumor cases (100%). SYT-SSX fusion transcript was detected in 18 tumor cases (90%). In 18 SYT-SSX positive SS cases, there are 12 present SYT-SSX1 fusion transcript and 6 present SYT-SSX2 fusion transcript. SYT-SSX1 fusion transcript can be seen in 9 monophasic SS and 3 biphasic SS. In 6 SYT-SSX2 positive SS cases, 4 were monophasic SS and 2 were biphasic. Conclusion: Detection of SYT-SSX fusion transcripts in FFPE tissues for diagnosis of SS is feasible and sensitive. Subtypes of SYT-SSX fusion gene may provide prognosis information. 展开更多
关键词 synovial sarcoma SYT-SSX fusion gene RT-PCR
下载PDF
Identification of cancer gene fusions based on advanced analysis of the human genome or transcriptome
6
作者 Lu Wang 《Frontiers of Medicine》 SCIE CSCD 2013年第3期280-289,共10页
Many gene fusions have been recognized as important diagnostic and/or prognostic markers in human malignancies.In recent years,novel gene fusions have been identified in cases without prior knowledge of the genetic ba... Many gene fusions have been recognized as important diagnostic and/or prognostic markers in human malignancies.In recent years,novel gene fusions have been identified in cases without prior knowledge of the genetic background.Accompanied by a powerful computational data analysis method,new genome-wide screening approaches were used to detect cryptic genomic aberrations.This review focused on advanced genome-wide screening approaches in fusion gene identification,such as microarray-based approaches,next-generation sequencing,and NanoString nCounter gene expression system.The fundamental rationale and strategy for fusion gene identification using each biotech platform are also discussed. 展开更多
关键词 gene fusion CANCER MICROARRAY next-generation sequencing NanoString nCounter system
原文传递
Construction of the recombinant expression vector for CD80-IgG fusion gene and its expression in Chinese hamster ovary cells
7
作者 WEI HE FANG LIU +3 位作者 LING BO LIU MIN ZHANG ZHONG BO HU PING ZOU 《Journal of Microbiology and Immunology》 2005年第4期293-300,共8页
To construct the recombinant expression functionally in Chinese hamster ovary cells in order vector for CD80-IgG fusion gene and to express it to be used as an effective method to eliminate the immune escape of leukem... To construct the recombinant expression functionally in Chinese hamster ovary cells in order vector for CD80-IgG fusion gene and to express it to be used as an effective method to eliminate the immune escape of leukemic cells, the cDNA encoding the signal and extracellular domains of murine CD80 was generated by PCR amplification from plasmid pcDNMB7 containing the full length cDNA of murine CD80 and those of murine IgG1, in which the Fc fragment was obtained through RT-PCR amplification from murine spleen cells. These two cDNAs were then cloned in tandem into eukaryotic expression vector pcDNA3.0 and the resultant recombinant plasmid pcDNA/CD80-IgG was then transfected to Chinese hamster ovary cells with liposome transfection reagent. The cell clones constitutively expressing CD80-IgG fusion protein were obtained by G418 screening. Western blotting and dot ELISA assay were used to detect the expression of the fusion protein in the supernatants of these cells. Meanwhile, the fusion protein expressed was then purified with affinity chromatography, and its biological activity was demonstrated by flow cytometry, MTr colorimetry and ELISA assay. The experimental resuits showed that these two inserts were successfully cloned into plasmid pcDNA3.0, and the highly purified fusion protein was obtained. This fusion protein was proved to be able to upregulate the density of CD80 on leukemic cells, deliberately promote the proliferative reactions of mouse allogenic lymphocytes and increase the killing activity against WEHI-3 cells from 49.7 % up to 84.6 %. In addition, this fusion protein could also enhance the IL-2 secretion from allogenic lymphocytes activated by tumorspecific antigens. It is concluded that the recombinant vector constructed can be functionally expressed in the mammalian cells, thus providing a solid foundation for the further investigation on the mechanism to eliminate the immune escape of leukemic cells in vivo. 展开更多
关键词 gene fusion Recombinant fusion proteins Immune escape Immunotherapy
下载PDF
Transgenic rice expressing a novel phytase-lactoferricin fusion gene to improve phosphorus availability and antibacterial activity 被引量:4
8
作者 WANG Zuo-ping DENG Li-hua +4 位作者 WENG Lü-shui DENG Xiang-yang FU Xi-qin XIN Ye-yun XIAO Guo-ying 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2017年第4期774-788,共15页
The developing trends of livestock production are efficiency availability of phytate phosphorus and abuse of antibiotics. safety and sustainability, which face two major challenges: low As a solution phytases and ant... The developing trends of livestock production are efficiency availability of phytate phosphorus and abuse of antibiotics. safety and sustainability, which face two major challenges: low As a solution phytases and antimicrobial peptides are applied as feed additives. However, phytases and antimicrobial peptides are susceptible to proteases, costly by fermentation and potential toxic to production hosts. We transformed an optimized phytase-lactoferricin fusion gene PhyLfdriven by an en- dosperm-specific promoter Gt13aP and Bar (bialaphos resistance) gene as a selection maker into rice. The Bar and PhyLf genes were integrated into the rice genome, stably inherited and expressed. Their phosphinothricin acetyl transferase (PAT) protein content oftransgenic plants with glufosinate resistance varied between 50.45-93.39 IJg g-l. Fusion protein expressed especially in the seeds of transgenic rice had a summit phytase activity at 32.30 U g-l, which increased by 61.71-fold com- pared to the control/check group (CK) and 7.54-fold compared to un-optimized transgenic plant. The highest inorganic phosphorus (Pi) content of the transgenic seeds reached 13.15 mg g-~, increased by 12.77-fold compared to that of CK. Preliminary antibacterial experiments showed that the enterokinase hydrolysate product of fusion protein could inhibit the growth of Escherichia coil DH5a. These results indicated that the protein PhyLf has the potential to increase availability of feed phytate phosphorus, improve consumer's immunity and reduce the use of antibiotics. 展开更多
关键词 RICE PHYTASE antimicrobial peptide LACTOFERRICIN fusion gene Bar gene herbicide resistance
下载PDF
Cloning of α-β fusion gene from Clostridium perfringens and its expression 被引量:5
9
作者 Jia-Ning Bai Yan Zhang Bao-Hua Zhao 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第8期1229-1234,共6页
AIM: To study the cloning of α-β fusion gene from Closindium perfringens and the immunogenidty of 0-6 fusion expression. METHODS: Cloning was accomplished after PCR amplification from strains NCTC64609 and C58-1 o... AIM: To study the cloning of α-β fusion gene from Closindium perfringens and the immunogenidty of 0-6 fusion expression. METHODS: Cloning was accomplished after PCR amplification from strains NCTC64609 and C58-1 of the protective antigen genes of α-toxin and β-toxin. The fragment of the gene was cloned using plasmid pZCPAB. This fragment coded for the gene with the stable expression of α-β fusion gene binding. In order to verify the exact location of the α-β fusion gene, domain plasmids were constructed. The two genes were fused into expression vector pBV221. The expressed α-β fusion protein was identified by ELISA, SDS-PAGE, Western blotting and neutralization assay. RESULTS: The protective co-toxin gene (cpa906) and the β-toxin gene (cpb930) were obtained. The recombinant plasmid pZCPAB carrying α-β fusion gene was constructed and transformed into BL21(DE3). The recombinant strain BL21(DE3)(pZCPAB) was obtained. After the recombinant strain BL21(DE3)(pZCPAB) was induced by 42℃, its expressed product was about 22.14% of total cellular protein at SDS-PAGE and thin-layer gel scanning analysis. Neutralization assay indicated that the antibody induced by immunization with α-βfusion protein could neutralize the toxicity of α-toxin and β-toxin. CONCLUSION: The obtained α-toxin and β-toxin genes are correct. The recombinant strain BL21(DE3)(pZCPAB) could produce α-β fusion protein. This protein can be used for immunization and is immunogenic. The antibody induced by immunization with α-β fusion protein could neutralize the toxicity of α-toxin and β-toxin. 展开更多
关键词 Clostridium perfringens α-β fusion gene Cloning and expression
下载PDF
Stable Expression of Hantavirus H8205 Strain G1/IL-2 Gene and Immune Protection of the Fusion Gene 被引量:1
10
作者 熊颖 袁媛 +2 位作者 贾珉 余冰 黄汉菊 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2007年第2期124-127,共4页
To explore the feasibility of stable expression of Hantavirus H8205 strain G1 segment and human IL-2 fusion gene in Vero cells, and to examine the immune protection effects on mice vaccinated with this recombinant euk... To explore the feasibility of stable expression of Hantavirus H8205 strain G1 segment and human IL-2 fusion gene in Vero cells, and to examine the immune protection effects on mice vaccinated with this recombinant eukaryotic expression vector containing Hantavirus G1 gene and IL-2 gene. With the help of lipofectamine, the Vero cells were transfected with pcDNA3.1/HisB-IL-2-G1 and the positive cells were selected by G418. IFAT and SDS-PAGE elec- trophoresis were used to determine the stable transfection and expression of recombinant protein. Each mouse was inoculated with plasmids intramuscularly (i.m.) three times, 2 boosts were given at 2-week intervals, serum anti-hantavirus antibodies were detected by ELISA and neutralizing antibodies (NAb) were detected by Plaque Reduction Neutralization Test. The fusion protein expressed in Vero cells was 78 kD, corresponding to the estimated molecular size. The neutralizing antibody titers of mice with pcDNA3.1/HisB-IL-2-G1 were 1:20-1:80. IL-2/G1 fusion gene could be transferred in Vero cells and stably express the fusion protein. Specific humeral immune responses in mice can be induced with the recombinant eukaryotic expression vector containing the fusion gene, which lays the foundation for further development of therapeutic HTNV vaccine. 展开更多
关键词 HANTAVIRUS fusion gene stable expression immune effect
下载PDF
Contruction of the Genetic Engineering Strain Expressed Nontoxic ST_1-LT_B Fusion Protein Against Enterotoxigenic Eschenichia coli 被引量:1
11
作者 BAIJia-ning SUNYi-min BIANYan-qing ZHAOBao-hua 《Agricultural Sciences in China》 CAS CSCD 2004年第7期535-540,共6页
Thermostable enterotoxinⅠ(ST1) mutant genes and thermolabile enterotoxin B subunit (LTB)genes were amplified by PCR from plasmids of Eschenichia coli C83902. The recombinantexpression plasmid pZST3LTB containing ST1-... Thermostable enterotoxinⅠ(ST1) mutant genes and thermolabile enterotoxin B subunit (LTB)genes were amplified by PCR from plasmids of Eschenichia coli C83902. The recombinantexpression plasmid pZST3LTB containing ST1-LTB fusion gene was constructed by recombinantDNA technique and then transformed into Escherichia coli BL21(DE3). The ST1-LTB fusionprotein was highly expressed in recombinant strain BL21(DE3)(pZST3LTB) and the fusionprotein was about 38.53% of total cellular protein by SDS-PAGE and thin-layer gelscanning analysis. More important, mice immunized with crude preparation containing thefusion protein inclusion bodies or inactivated recombinant strain produced antibodiesthat were able to recognize ST1 in vitro. These sera antibodies were able to neutralizethe biological activity of native ST1 in the suckling mouse assay. Hence the ST1-LTBfusion protein was nontoxic and immunogenic, the constructed recombinant strain BL21(DE3)(pZST3LTB) could be used as a candidate of vaccine strain. 展开更多
关键词 Thermostable enterotoxinⅠgene Thermolabile enterotoxin B subunit gene fusion gene fusion protein gene expression
下载PDF
Fusion genes in solid tumors: an emerging target for cancer diagnosis and treatment 被引量:4
12
作者 Brittany C.Parker Wei Zhang 《Chinese Journal of Cancer》 SCIE CAS CSCD 2013年第11期594-603,共10页
Studies over the past decades have uncovered fusion genes, a class of oncogenes that provide immense diagnostic and therapeutic advantages because of their tumor-specific expression. Originally associated with hemotol... Studies over the past decades have uncovered fusion genes, a class of oncogenes that provide immense diagnostic and therapeutic advantages because of their tumor-specific expression. Originally associated with hemotologic cancers, fusion genes have recently been discovered in a wide array of solid tumors, including sarcomas, carcinomas, and tumors of the central nervous system. Fusion genes are attractive as both therapeutic targets and diagnostic tools due to their inherent expression in tumor tissue alone. Therefore, the discovery and elucidation of fusion genes in various cancer types may provide more effective therapies in the future for cancer patients. 展开更多
关键词 融合基因 癌症患者 治疗性 实体瘤 肿瘤组织 中枢神经系统 诊断工具 特异性表达
下载PDF
Detection of fusion gene in cell-free DNA of a gastric synovial sarcoma 被引量:5
13
作者 Shinpei Ogino Hirotaka Konishi +10 位作者 Daisuke Ichikawa Junichi Hamada Katsutoshi Shoda Tomohiro Arita Shuhei Komatsu Atsushi Shiozaki Kazuma Okamoto Sanae Yamazaki Satoru Yasukawa Eiichi Konishi Eigo Otsuji 《World Journal of Gastroenterology》 SCIE CAS 2018年第8期949-956,共8页
Synovial sarcoma(SS) is genetically characterized by chromosomal translocation, which generates SYT-SSX fusion transcripts. Although SS can occur in any body part, primary gastric SS is substantially rare. Here we des... Synovial sarcoma(SS) is genetically characterized by chromosomal translocation, which generates SYT-SSX fusion transcripts. Although SS can occur in any body part, primary gastric SS is substantially rare. Here we describe a detection of the fusion gene sequence of gastric SS in plasma cell-free DNA(cf DNA). A gastric submucosal tumor was detected in the stomach of a 27-year-old woman and diagnosed as SS. Candidate intronic primers were designed to detect the intronic fusion breakpoint and this fusion sequence was confirmed in intron 10 of SYT and intron 5 of SSX2 by genomic polymerase chain reaction(PCR) and direct sequencing. A locked nucleic acid(LNA) probe specificto the fusion sequence was designed for detecting the fusion sequence in plasma and the fusion sequence was detected in preoperative plasma cfD NA, while not detected in postoperative plasma cfD NA. This technique will be useful for monitoring translocation-derived diseases such as SS. 展开更多
关键词 fusion gene GASTRIC SYNOVIAL SARCOMA PLASMA Cell free DNA
下载PDF
INHIBITION OF APOPTOSIS BY bcr-abl FUSION GENE IN K562 CELLS
14
作者 王春红 孙秉中 袁跃传 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1999年第3期174-176,共3页
Objective: To investigate the effect of bcr-abl fusion gene on CML cell apoptosis. Methods: Apoptosis of ex-vivo cultured K562 cells were observed after exposure to synthetic 18 mer antisense oligodeoxynucleotide comp... Objective: To investigate the effect of bcr-abl fusion gene on CML cell apoptosis. Methods: Apoptosis of ex-vivo cultured K562 cells were observed after exposure to synthetic 18 mer antisense oligodeoxynucleotide complementary to the bcr-abl junction (b3a2). Results: Apoptosis of K562 cells was significantly increased associated with inhibition of bcr-abl expression. Conclusion: bcr-abl fusion gene formation due to chromosome translocation may be the major mechanism of CML via inhibition of apoptosis. 展开更多
关键词 chronic myeloid leukemia bcr-abl fusion gene APOPTOSIS Antisense oligodeoxynucleotides
下载PDF
Detection of TMPRSS2:ERG fusion gene in circulating prostate cancer cells
15
作者 Xueying Mao Greg Shaw +9 位作者 Sharon Y. James Patricia Purkis Sakunthala C. Kudahetti Theodora Tsigani Saname Kia Bryan D. Young R. Tim D. Oliver Dan Berney David M. Prowse Yong-Jie Lu 《Asian Journal of Andrology》 SCIE CAS CSCD 2008年第3期467-473,共7页
Aim: To investigate the existence of TMPRSS2:ERG fusion gene in circulating tumor cells (CTC) from prostate cancer patients and its potential in monitoring tumor metastasis. Methods- We analyzed the frequency of T... Aim: To investigate the existence of TMPRSS2:ERG fusion gene in circulating tumor cells (CTC) from prostate cancer patients and its potential in monitoring tumor metastasis. Methods- We analyzed the frequency of TMPRSS2: ERG and TMPRSS2:ETV1 transcripts in 27 prostate cancer biopsies from prostatectomies, and TMPRSS2:ERG transcripts in CTC isolated from 15 patients with advanced androgen independent disease using reverse transcription polymerase chain reaction (RT-PCR). Fluorescence in situ hybridization (FISH) was applied to analyze the genomic truncation of ERG, which is the result of TMPRSS2:ERG fusion in 10 of the 15 CTC samples. Results: TMPRSS2: ERG transcripts were found in 44% of our samples, but we did not detect expression of TMPRSS2:ETV1. Using FISH analysis we detected chromosomal rearrangements affecting the ERG gene in 6 of 10 CTC samples, including 1 case with associated TMPRSS2:ERG fusion at the primary site. However, TMPRSS2:ERG transcripts were not detected in any of the 15 CTC samples, including the 10 cases analyzed by FISH. Conclusion: Although further study is required to address the association between TMPRSS2:ERG fusion and prostate cancer metastasis, detection of genomic truncation of the ERG gene by FISH analysis could be useful for monitoring the appearance of CTC and the potential for prostate cancer metastasis. 展开更多
关键词 TMPRSS2:ERG fusion gene prostate cancer METASTASIS circulating tumor cells fluorescence in situ hybridization polymerase chain reaction
下载PDF
Cloned s-Lap Gene Coding Area, Expression and Localization of s-Lap/GFP Fusion Protein in Mammal Cells
16
作者 SONGYi-shu SONGZhi-yu +4 位作者 LIHong-jun WuYin BAOYong-li TANDa-peng LIYu-xin 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2005年第3期298-300,共3页
s-Lap is a new gene sequence from pig retinal pigment epithelial(RPE) cells, which was found and cloned in the early period of apoptosis of RPE cells damaged with visible light. We cloned the coding area sequence of t... s-Lap is a new gene sequence from pig retinal pigment epithelial(RPE) cells, which was found and cloned in the early period of apoptosis of RPE cells damaged with visible light. We cloned the coding area sequence of the novel gene of s-Lap and constructed its recombinant eukaryotic plasmid pcDNA3.1-GFP/s-lap with the recombinant DNA technique. The expression and localization of s-lap/GFP fusion protein in CHO and B_~16 cell lines were studied with the instantaneously transfected pcDNA3.1-GFP/s-lap recombinant plasmid. ~s-Lap/GFP fusion protein can be expressed in CHO and B_~16 cells with a high rate expression in the nuclei. 展开更多
关键词 s-Lap gene fusion protein Mammal cell EXPRESSION LOCALIZATION
下载PDF
VISUALIZATION OF HEAD AND NECK CANCER MODELS WITH A TRIPLE FUSION REPORTER GENE
17
作者 YING ZHENG QIAOYA LIN +2 位作者 HONGLIN JIN JUAN CHEN ZHIHONG ZHANG 《Journal of Innovative Optical Health Sciences》 SCIE EI CAS 2012年第4期48-56,共9页
The development of experimental animal models for head and neck tumors generally rely on the biol uminescence imaging to achieve the dynamic monitoring of the tumor growth and metastasis due to the complicated anatomi... The development of experimental animal models for head and neck tumors generally rely on the biol uminescence imaging to achieve the dynamic monitoring of the tumor growth and metastasis due to the complicated anatomical structures.Since the bioluminescence imaging is largely affected by the intracellular luciferase expression level and external D-luciferin concentrations,its imaging accuracy requires further confirmation.Here,a new triple fusion reportelr gene,which consists of a herpes simplex virus type 1 thymidine kinase(TK)gene for radioactive imaging,a far-red fuorescent protein(mLumin)gene for fuorescent imaging,and a firefly luciferase gene for bioluminescence imaging,was introduced for in vrivo observation of the head and neck tumors through multi-modality imaging.Results show that fuorescence and bioluminescence signals from mLumin and luciferase,respectively,were clearly observed in tumor cells,and TK could activate suicide pathway of the cells in the presence of nucleotide analog-ganciclovir(GCV),demonstrating the effecti veness of individual functions of each gene.Moreover,subcutaneous and metastasis animal models for head and neck tumors using the fusion reporter gene-expressing cell lines were established,allowing multi-modality imaging in vio.Together,the established tumor models of head and neck cancer based on the newly developed triple fusion reporter gene are ideal for monitoring tumor growth,assessing the drug therapeutic efficacy and verifying the effec-tiveness of new treatments. 展开更多
关键词 Head and neck cancer tumor metastasis model three fusion reporter gene far-red fluorescent protein frefly luciferase multi-modality imaging
下载PDF
Expression of GST-IL-1 fusion gene
18
作者 陈梅红 王字玲 +3 位作者 邓健蓓 赵忠良 陈南春 苏成芝 《Journal of Medical Colleges of PLA(China)》 CAS 1996年第2期79-83,共5页
Two GST-IL-1 fusion genes were constructed by inserting different cDNA fragments of human interleukin1 (IL-1) into the 3'-terminus of GST gene in the fusion protein expression vector pGEX-4T. After IPTG induction ... Two GST-IL-1 fusion genes were constructed by inserting different cDNA fragments of human interleukin1 (IL-1) into the 3'-terminus of GST gene in the fusion protein expression vector pGEX-4T. After IPTG induction ,SDS-PAGE was employed to detect the gene expression. No corresponding protein encoded by GST gene fused with the whole-length 816 bp IL-1 cDNA was observed, nor was free GST protein. However, the fusion protein of GST and IL-1 cDNA without the 189 bp at the 5'- terminus was detected, amounting to 30% of the total bacterial protein expressed. This might suggest that the sequence of 1-189 bp of IL-1 cDNA affected the expression of the fusion gene. That is to say, the downstream sequence distant from the translation start codon AUG in the target gene could significantly affect the expression of the fusion gene. 展开更多
关键词 gene EXPRESSION fusion PROTEIN INTERLEUKIN-1 GLUTATHIONE-S-TRANSFERASE
下载PDF
Fusion of EGFP and porcine α 1,3GT genes decrease GFP expression
19
作者 Yongxiang Zhao Jing Tang +11 位作者 Qin Yao Yuan Zhou Huange Zhao Xiaoyun Zeng Jiaqi Shi Guorong Luo Xiaoxun Xie Sufang Zhou Zuguo Liu Xiaoling Lu Donghai Lin Jianming Liu 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2010年第12期925-929,共5页
Objective:To investigate the effect of fusion proteins expressed by the fused gene of porcineα1,3 galactosyltransierase(α1,3 GT) and enhanced green fluorescent protein(EGFP) on the green fluorescence intensity of EG... Objective:To investigate the effect of fusion proteins expressed by the fused gene of porcineα1,3 galactosyltransierase(α1,3 GT) and enhanced green fluorescent protein(EGFP) on the green fluorescence intensity of EGFP.Methods:The fragment containingα1.3GT was firstly recovered after the pcDNA3.1-α1.3GT recombinant vector were digested with BamHl and EcoRI,and then,the resultant fragment was ligated to the pEGFP-N1 vector which was also digested with the same enzymes.The new recombinant eukaryotic expression pEGFP/a 1,3GT vector was obtained and sequenced.The pEGFP/α1,3GT was used to transfect human lung carcinoma cells A549 and HEKC 293FT,and the expression of EGFP was quantitatively analyzed by fluorescent microscope and flow cytometry.Results:The positive percentage of A549 was 80.5%,and that of 293 FT was 86.5%48 hours after the two cell lines both were transfected by pEGFP-N1.The positive percentage of A549 was 75.8%,and that of 293 FT was 81.2%48 hours after the two cell lines were transfected by pEGFP/α1.3GT.The mean fluorescence intensities of A549 transfected with pEGFP-N1 and pEGFP/α1.3GT were 1.21 and 0.956,respectively when compared with that of A549 without transfection.Meanwhile,the those of the 293FT that were transfected with pEGFP-N1 and pEGFP/αl,3GT were 7.66 and 1.00.respectively when compared with that of 293FT cells without transfection.Conclusions:These results suggested that the expression of EGFP gene fused with porcineα1,3GT gene was partly inhibited. 展开更多
关键词 Enhanced green FLUORESCENT protein Porcineα1.3 GALACTOSYLTRANSFERASE fusion gene Fluorescence intensity
下载PDF
Xp11.2易位/TFE3基因融合相关性肾细胞癌的超声表现 被引量:1
20
作者 陈娟 蔡迪明 《中国医学影像学杂志》 CSCD 北大核心 2024年第2期180-184,共5页
目的探讨Xp11.2易位/TFE3基因融合相关性肾细胞癌(Xp11.2/TFE3-tRCC)的超声表现。资料与方法回顾性分析四川大学华西医院2009年4月—2022年2月手术经病理证实Xp11.2/TFE3-tRCC的10例患者,收集其超声图像及临床资料。观察肿块的边界、形... 目的探讨Xp11.2易位/TFE3基因融合相关性肾细胞癌(Xp11.2/TFE3-tRCC)的超声表现。资料与方法回顾性分析四川大学华西医院2009年4月—2022年2月手术经病理证实Xp11.2/TFE3-tRCC的10例患者,收集其超声图像及临床资料。观察肿块的边界、形态、内部回声、血流信号情况及超声造影表现等。结果共纳入10例肿块,其中4例行超声造影。Xp11.2/TFE3-tRCC的超声表现呈多样性及多变性,肿块好发于髓质(5例)。常规超声上6例表现为类似良性病灶的边界较清楚、形态较规则的实性结节,彩色多普勒显示5例为点状血流信号。超声造影上则更倾向于恶性肿块(3例),具体表现为不均匀强化,多伴有周边不均匀增强环。结论Xp11.2/TFE3-tRCC的常规超声表现多倾向良性,而超声造影则表现为恶性病灶,因此超声造影可作为诊断可疑Xp11.2/TFE3-tRCC的潜在方法。 展开更多
关键词 肾细胞癌 超声检查 Xp11.2易位 TFE3基因融合 病理学 外科
下载PDF
上一页 1 2 122 下一页 到第
使用帮助 返回顶部