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Characteristics of corneal dystrophies:a review from clinical,histological and genetic perspectives 被引量:8
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作者 Ze-Nan Lin Jie Chen Hong-Ping Cui 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2016年第6期904-913,共10页
Corneal dystrophy is a common type of hereditary corneal diseases. It includes many types, which have varied pathology, histology and clinical manifestations. Recently, the examination techniques of ophthalmology and ... Corneal dystrophy is a common type of hereditary corneal diseases. It includes many types, which have varied pathology, histology and clinical manifestations. Recently, the examination techniques of ophthalmology and gene sequencing advance greatly, which do benefit to our understanding of these diseases. However, many aspects remain still unknown. And due to the poor knowledge of these diseases, the results of the treatments are not satisfactory. The purpose of this review was to summarize the clinical, histological and genetic characteristics of different types of corneal dystrophies. 展开更多
关键词 corneal dystrophy CLINIC HISTOLOGY genemutation
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Clinical and biological characteristics of colorectal cancer with familial predisposition
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作者 吴保平 张亚历 +2 位作者 周殿元 高春芳 赖卓胜 《Journal of Medical Colleges of PLA(China)》 CAS 2001年第2期90-93,共4页
Objective:To evaluatethemicrosatelliteinstability(MSI),expressionof mismatchrepair(MMR)gene(hMLH1,hMSH2)andproliferationkineticsincolorectalcancer(CRC)withfamilialpredisposition.Method:Forty-sixcasesof CRC were studie... Objective:To evaluatethemicrosatelliteinstability(MSI),expressionof mismatchrepair(MMR)gene(hMLH1,hMSH2)andproliferationkineticsincolorectalcancer(CRC)withfamilialpredisposition.Method:Forty-sixcasesof CRC were studiedusingsilverstainingpolymerasechainreaction-singlestrandconformation polymorphism(PCR-SSCP)technique,streptavidin-peroxidase(SP)immunohistochemicalmethodand flowcyto-metry.Results:In CRCpatientswithfamilialpredisposition,theMSI-positiveratewas higherthanin sporadicCRC(P<0.05).FamilialpredispositionandpositiveMSIwerestronglyrelatedto earlyageof canceronset,theproclivity for proximalcoloniccancer,poordifferentiatedandextracolorectalnmalignancies(P<0.01,P<0.05).Theincidence of negativeexpressionof hMLH1intumortissueandhMLH1,hMSH2innormalcolorectaltissueswassignificantly higher(P<0.05).Thenegativeexpressionof hMLH1togetherwithhMSH2was relatedwithpositiveMSI(P<0.05).InMSI-positiveCRCcells,theproliferationcellnucleusantigen(PCNA)expression,proliferationindexandS-phase cellsdecreasedobviously(P<0.01,P<0.05).Conclusion:In CRCwithfamilialpredisposition,MSI mightbe an importantcontributor.Therateof hMLH1andhMSH2mutationincreasedintumorandnormaltissue,andtheproli-ferationactivityof theircancercellwaslower. 展开更多
关键词 COLORECTALCANCER familialpredisposition microsatelliteinstability mismatchrepairgene genemutation GENEEXPRESSION CELLPROLIFERATION
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