Mouse TGF-β1 gene was microinjected into male pronuclei of F2 hybrid fertilized eggs obtained by mating CSJLF1 and C57BL/6J inbred strains to generate transgenic mice with over-expressed TGF-β1 gene. The rate of fou...Mouse TGF-β1 gene was microinjected into male pronuclei of F2 hybrid fertilized eggs obtained by mating CSJLF1 and C57BL/6J inbred strains to generate transgenic mice with over-expressed TGF-β1 gene. The rate of founder production is 31% and Southern blot analysis of founder mice tail DNAS gave an integration efficiency of 33%. TGF-β1 gene could be stably integrated to the chromosomes of transgenic mice and transmitted to their progeny at a rate of 33% in the second generation. Dot blot analysis of tail RNA of some transgenic mice indicated a moderate expression of the transgene. The most interesting finding of the present work is the striking deviation from the normal male:female sex ratio in transgenic mice,with an average ratio of 6.7:1. The possible nature of the predominance of male sex in transgenic mice overexpressing TGF-β1 is discussed.展开更多
Understanding physiological responses in saline agriculture may facilitate wheat breeding programs.Based on a screening test,the Ningmai-14(NM-14)and Yangmai-23(YM-23)wheat cultivars were selected for further experime...Understanding physiological responses in saline agriculture may facilitate wheat breeding programs.Based on a screening test,the Ningmai-14(NM-14)and Yangmai-23(YM-23)wheat cultivars were selected for further experiments to understand the underlying salinity tolerance mechanism.This study investigated the effects of five salinity levels such as Control(CK)=0(without NaCl stress),S1=0.20%,S2=0.25%,S3=0.30%and S4=0.35%of NaCl concentrations of soil on wheat plants.The results showed that increased salinity concentration reduced the growth and yield of wheat cultivars(NM-14 and YM-23).However,YM-23(12.7%)yielded more than NM-14 at maximum salinity stress.The higher salinity(S4)increased the concentration of Na^(+)(4.3 to 5.8-fold)and P contents(2.5 to 2.2-fold),while reducing the average concentrations of K^(+),Cu,and K^(+)/Na^(+)ratio.The higher salinity(S4)reduced the spikelet length by 21.35%(followed by grain spike−1),and the starch content by 18.81%.In the YM-23 cultivar,higher salinity increased superoxide dismutase(SOD),total antioxidant capacity(TAC),and amylase.Compared to NM-14,induced expression of TaYUC2,6,and TaGA13ox,20ox genes were recorded in YM-23.Similarly,in YM-23 the stress-specific genes such as TaHSP70,90 were enhanced whereas,TaSOS1,2 were suppressed.Overall,our study revealed that salt tolerant cultivars modulate hormonal and antioxidant activities,thus maintaining high growth.展开更多
The current study proposes a novel technique for feature selection by inculcating robustness in the conventional Signal to noise Ratio(SNR).The proposed method utilizes the robust measures of location i.e.,the“Median...The current study proposes a novel technique for feature selection by inculcating robustness in the conventional Signal to noise Ratio(SNR).The proposed method utilizes the robust measures of location i.e.,the“Median”as well as the measures of variation i.e.,“Median absolute deviation(MAD)and Interquartile range(IQR)”in the SNR.By this way,two independent robust signal-to-noise ratios have been proposed.The proposed method selects the most informative genes/features by combining the minimum subset of genes or features obtained via the greedy search approach with top-ranked genes selected through the robust signal-to-noise ratio(RSNR).The results obtained via the proposed method are compared with wellknown gene/feature selection methods on the basis of performance metric i.e.,classification error rate.A total of 5 gene expression datasets have been used in this study.Different subsets of informative genes are selected by the proposed and all the other methods included in the study,and their efficacy in terms of classification is investigated by using the classifier models such as support vector machine(SVM),Random forest(RF)and k-nearest neighbors(k-NN).The results of the analysis reveal that the proposed method(RSNR)produces minimum error rates than all the other competing feature selection methods in majority of the cases.For further assessment of the method,a detailed simulation study is also conducted.展开更多
AIM To investigate the role of genetic variants of angiotensin converting enzyme(ACE) and angiotensinogen(AGT) genes in the antiproteinuric efficacy of ACE inhibitor therapy in diabetic nephropathy(DN) patients.METHOD...AIM To investigate the role of genetic variants of angiotensin converting enzyme(ACE) and angiotensinogen(AGT) genes in the antiproteinuric efficacy of ACE inhibitor therapy in diabetic nephropathy(DN) patients.METHODS In the present study, 270 type 2 diabetes mellitus patients with nephropathy were enrolled and treated with ACE inhibitor(ramipril) and followed at 6 mo for renal function and albumin excretion by estimating serum creatinine, end stage renal disease, and albumin/creatinine ratio(ACR) in urine. Genotyping of ACE I/D and AGT M235 T polymorphisms were performed by using primer specific polymerase chain reaction(PCR) and PCR-RFLP techniques, respectively. RESULTS Forty-eight percent of DN patients(responders) benefited with respect to proteinuria from ACE inhibitor therapy at 6 mo follow-up. A significant reduction in ACR was observed after 6 mo treatment with ACE inhibitor irrespective of whether DN patients were micro-albuminuric(≥ 30 and < 300 mg/g creatinine) or macro-albuminuric(≥ 300 mg/g creatinine) at the time of enrollment. However, macro-albuminuric patients(55%) showed better response to therapy. A reduction in urinary ACR was found independent of genotypes of ACE I/D and AGT M235 T polymorphisms although macro-albuminuric patients having TT genotype showed statistically insignificant increased response(72%). CONCLUSION ACE inhibitor therapy reduced urinary ACR by ≥ 30% in 50% of DN patients and the response is independent of ACE I/D and AGT M235 T polymorphisms.展开更多
In this paper, based on binomial formula, a law of genetic inheritance is proposed, and it would have certain reference value for future genetic inheritance research. Besides, a recursive algorithm for recessive inher...In this paper, based on binomial formula, a law of genetic inheritance is proposed, and it would have certain reference value for future genetic inheritance research. Besides, a recursive algorithm for recessive inheritance is also proposed. Given any two of the male parent, female parent and filial generation, the genomic information of the third one can be calculated uniquely.展开更多
Palm oil is of great potential as one of the sustainable alternatives to fish oil(FO) in aquafeeds.In this present study,five isonitrogenous diets(32% crude protein) with elevated palm oil levels of 0%,2%,4%,6% and 8%...Palm oil is of great potential as one of the sustainable alternatives to fish oil(FO) in aquafeeds.In this present study,five isonitrogenous diets(32% crude protein) with elevated palm oil levels of 0%,2%,4%,6% and 8% were used during an 8-week feeding trial to evaluate its effects on RNA/DNA ratio and lipoprotein lipase(LPL) and Myo D m RNA expressions in muscle of Oreochromis niloticus.The results showed that RNA,DNA content as well as ratio of RNA to DNA were significantly affected(P < 0.05),in each case the highest was recorded in fish group subjected to 6% palm oil level.There was a strong positive correlation between nucleic acid concentration(RNA concentration and RNA:DNA ratio) and specific growth rate(SGR),protein efficiency ratio(PER),while a negative correlation existed between nucleic acid concentration(RNA concentration and RNA:DNA ratio) and feed conversion ratio(FCR).The m RNA expressions of LPL and Myo D in muscle were not significantly affected by the different palm oil levels,although the highest expression was observed in fish fed with 6% palm oil level.There also existed a strong positive correlation between the m RNA expression of LPL,Myo D and SGR,PER,while their correlation with FCR was negative.In conclusion,elevated palm oil affected the RNA,DNA concentration as well as RNA/DNA ratio significantly,although the m RNA expression of LPL and Myo D were not affected significantly by elevated palm oil levels.展开更多
Objective： To discuss strategies and methods of normalization on how to deal with and analyze data for different chips with the combination of statistics, mathematics and bioinformatics in order to find significant d...Objective： To discuss strategies and methods of normalization on how to deal with and analyze data for different chips with the combination of statistics, mathematics and bioinformatics in order to find significant difference genes. Methods： With Excel and SPSS software, high or low density chips were analyzed through total intensity normalization （TIN） and locally weighted linear regression normalization （LWLRN）. Results： These methods effectively reduced systemic errors and made data more comparable and reliable. Conclusion： These methods can search the genes of significant difference, although normalization methods are being developed and need to be improved further. Great breakthrough will be obtained in microarray data normalization analysis and transformation with the development of non-linear technology, software and hardware of computer.展开更多
Objective:To study the value of serum pepsinogenⅠ andⅡ ratio (PGR) for evaluating the malignant biological behaviors in gastric cancer lesions.Methods:The patients with gastric cancer who underwent radical surgery i...Objective:To study the value of serum pepsinogenⅠ andⅡ ratio (PGR) for evaluating the malignant biological behaviors in gastric cancer lesions.Methods:The patients with gastric cancer who underwent radical surgery in 363 Hospital between July 2015 and February 2018 were selected as the gastric cancer group in the study, and the volunteers who had physical examination in 363 Hospital during the same period were selected as the control group. Serum was collected to measure pepsinogenⅠ and pepsinogenⅡ and then calculate the PGR;gastric cancer lesions were collected to measure the expression of oncogenes and angiogenesis genes.Results:Serum PGR level of the gastric cancer group was significantly lower than that of the control group, serum PGR level of the patients with moderately-highly differentiated gastric cancer was significantly higher than that of the patients with lowly differentiated gastric cancer, serum PGR level of the patients with TNM stage III gastric cancer was significantly lower than that of the patients with TNM stage I+II gastric cancer, and serum PGR level of the gastric cancer patients with lymph node metastasis was significantly lower than that of the gastric cancer patients without lymph node metastasis;PGR=3.8 was taken as the cutoff point, and p53 and TXNIP mRNA expression in the lesions of the gastric cancer patients with PGR < 3.8 were significantly lower than those of the gastric cancer patients with PGR > 3.8 while Bcl-2,β-catenin, Survivin, COX-2, HIF-1α, VEGF, c-Met and CNPY2 mRNA expression were significantly higher than those of the gastric cancer patients with PGR > 3.8.Conclusion:The decrease of PGR in serum of patients with gastric cancer is valuable for evaluating the pathological process and malignant biological behaviors.展开更多
Objective:To study the correlation between the ultrasonic elastic strain ratio of thyroid cancer and the malignant biological characteristics of cancer cells in the lesions.Methods:A total of 90 patients with thyroid ...Objective:To study the correlation between the ultrasonic elastic strain ratio of thyroid cancer and the malignant biological characteristics of cancer cells in the lesions.Methods:A total of 90 patients with thyroid cancer who accepted surgical treatment in our hospital between March 2015 and September 2016 were selected as the observation group, and 50 patients who received surgery in our hospital during the same period and were with clear pathological diagnosis of thyroid adenoma were selected as the control group. According to the median of ultrasonic elastic strain ratio, the observing group were further divided into high elastic strain ratio group and low elastic strain ratio group (n=45). RT-PCR was used to determine the mRNA expression of proliferation genes, invasion genes and autophagy genes in lesions of all groups of patients.Results: The mean elastic strain ratio of observation group was significantly lower than that of control group. Proliferation gene FOXA1 mRNA expression in lesions of low elastic strain ratio group and high elastic strain ratio group were higher than those of control group while PP4R1 and Smac mRNA expression were lower than those of control group, and as the elastic strain ratio decreased, the FOXA1 mRNA expression increased while PP4R1 and Smac mRNA expression decreased;invasion genes BCORL1, SATB1, Twist1 and CXCR4 mRNA expression in lesions of low elastic strain ratio group and high elastic strain ratio group were higher than those of control group, and as the elastic strain ratio decreased, the BCORL1, SATB1, Twist1 and CXCR4 mRNA expression increased;autophagy genes ARHI, Beclin1, LC3 and PTEN mRNA expression in lesions of low elastic strain ratio group and high elastic strain ratio group were lower than those of control group, and as the elastic strain ratio decreased, the ARHI, Beclin1, LC3 and PTEN mRNA expression decreased.Conclusion:The ultrasonic elastic strain ratio of thyroid cancer is negatively correlated with tumor cell proliferation and invasion activity, and positively correlated with autophagy activity.展开更多
As the most abundant living entities in the environment,viruses have been well recognized as crucial members in sustaining biogeochemical cycling.However,the significance of viruses in soil ecosystem multifunctionalit...As the most abundant living entities in the environment,viruses have been well recognized as crucial members in sustaining biogeochemical cycling.However,the significance of viruses in soil ecosystem multifunctionality remains under-explored.In this study,we used metagenomics and meta-viromics analysis to investigate the role of soil viruses in soil ecosystem functions under heavy,light,and no organochlorine pesticides(OCPs)contamination.In the three types of soil samples collected,lightcontaminated soils supported the highest level of multifunctionality,followed by heavy-contaminated soils and clean soils.Additionally,our results revealed a positive correlation between bacterial community evenness and multifunctionality index(p<0.05).Dominant bacterial species with biodegradation and stress resistance advantages exhibited higher abundance in OCPaffected soils,potentially playing a core functional supporting role.Furthermore,our results indicated that the species richness and diversity of bacteriophages were positively correlated with multifunctionality(p<0.05)in OCP-affected soils.Bacteriophages in OCP-affected soils regulate host metabolism and enhance soil ecosystem multifunctionality by infecting functional bacterial hosts and encoding AMGs related to soil element cycling.Our findings emphasize the potential effect of phages on ecosystem multifunctionality in contaminated soil,suggesting that phages may serve as contributors to soil ecology beyond bacteria and other microorganisms.Therefore,in polluted or constrained soils,further research could potentially translate phage communities and related ecological processes into artificial methods for application in soil pollution remediation or ecological restoration.展开更多
Petroleum hydrocarbons,mainly consisting of n-alkanes and polycyclic aromatic hydrocarbons(PAHs),are considered as priority pollutants and biohazards in the environment,eventually affecting the ecosystem and human hea...Petroleum hydrocarbons,mainly consisting of n-alkanes and polycyclic aromatic hydrocarbons(PAHs),are considered as priority pollutants and biohazards in the environment,eventually affecting the ecosystem and human health.Though many previous studies have investigated the change of bacterial community and alkane degraders during the degradation of petroleum hydrocarbons,there is still lack of understanding on the impacts of soil alkane contamination level.In the present study,microcosms with different n-alkane contamination(1%,3%and 5%)were set up and our results indicated a complete alkane degradation after 30 and 50 days in 1%-and 3%-alkane treatments,respectively.In all the treatments,alkanes with medium-chain length(C_(11)-C_(14))were preferentially degraded by soil microbes,followed by C27-alkane in 3%and 5%treatments.Alkane contamination level slightly altered soil bacterial community,and the main change was the presence and abundance of dominant alkane degraders.Thermogemmatisporaceae,Gemmataceae and Thermodesulfovibrionaceae were highly related to the degradation of C_(14)-and C_(27)-alkanes in 5%treatment,but linked to alkanes with medium-chain(C11-C18)in 1%treatment and C21-alkane in 3%treatment,respectively.Additionally,we compared the abundance of three alkane-monooxygenase genes,e.g.,alk_A,alk_P and alk_R.The abundance of alk_R gene was highest in soils,and alk_P gene was more correlated with alkane degradation efficiency,especially in 5%treatment.Our results suggested that alkane contamination level showed non-negligible effects on soil bacterial communities to some extents,and particularly shaped alkane degraders and degrading genes significantly.This study provides a better understanding on the response of alkane degraders and bacterial communities to soil alkane concentrations,which affects their biodegradation process.展开更多
文摘Mouse TGF-β1 gene was microinjected into male pronuclei of F2 hybrid fertilized eggs obtained by mating CSJLF1 and C57BL/6J inbred strains to generate transgenic mice with over-expressed TGF-β1 gene. The rate of founder production is 31% and Southern blot analysis of founder mice tail DNAS gave an integration efficiency of 33%. TGF-β1 gene could be stably integrated to the chromosomes of transgenic mice and transmitted to their progeny at a rate of 33% in the second generation. Dot blot analysis of tail RNA of some transgenic mice indicated a moderate expression of the transgene. The most interesting finding of the present work is the striking deviation from the normal male:female sex ratio in transgenic mice,with an average ratio of 6.7:1. The possible nature of the predominance of male sex in transgenic mice overexpressing TGF-β1 is discussed.
基金the National Natural Science Foundation of China(32101817)Jiangsu Agriculture Science and this work was funded by the National Natural Science Foundation of China(32101817)+3 种基金Jiangsu Agriculture Science and Technology Innovation Fund(CX(21)3111)the Natural Science Foundation of the Jiangsu Higher Education Institutions(21KJD210001)the Scientific and Technological Innovation Fund of Carbon Emissions Peak and Neutrality of Jiangsu Provincial Department of Science and Technology(BE2022304)the project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)for their financial support.
文摘Understanding physiological responses in saline agriculture may facilitate wheat breeding programs.Based on a screening test,the Ningmai-14(NM-14)and Yangmai-23(YM-23)wheat cultivars were selected for further experiments to understand the underlying salinity tolerance mechanism.This study investigated the effects of five salinity levels such as Control(CK)=0(without NaCl stress),S1=0.20%,S2=0.25%,S3=0.30%and S4=0.35%of NaCl concentrations of soil on wheat plants.The results showed that increased salinity concentration reduced the growth and yield of wheat cultivars(NM-14 and YM-23).However,YM-23(12.7%)yielded more than NM-14 at maximum salinity stress.The higher salinity(S4)increased the concentration of Na^(+)(4.3 to 5.8-fold)and P contents(2.5 to 2.2-fold),while reducing the average concentrations of K^(+),Cu,and K^(+)/Na^(+)ratio.The higher salinity(S4)reduced the spikelet length by 21.35%(followed by grain spike−1),and the starch content by 18.81%.In the YM-23 cultivar,higher salinity increased superoxide dismutase(SOD),total antioxidant capacity(TAC),and amylase.Compared to NM-14,induced expression of TaYUC2,6,and TaGA13ox,20ox genes were recorded in YM-23.Similarly,in YM-23 the stress-specific genes such as TaHSP70,90 were enhanced whereas,TaSOS1,2 were suppressed.Overall,our study revealed that salt tolerant cultivars modulate hormonal and antioxidant activities,thus maintaining high growth.
基金King Saud University for funding this work through Researchers Supporting Project Number(RSP2022R426),King Saud University,Riyadh,Saudi Arabia.
文摘The current study proposes a novel technique for feature selection by inculcating robustness in the conventional Signal to noise Ratio(SNR).The proposed method utilizes the robust measures of location i.e.,the“Median”as well as the measures of variation i.e.,“Median absolute deviation(MAD)and Interquartile range(IQR)”in the SNR.By this way,two independent robust signal-to-noise ratios have been proposed.The proposed method selects the most informative genes/features by combining the minimum subset of genes or features obtained via the greedy search approach with top-ranked genes selected through the robust signal-to-noise ratio(RSNR).The results obtained via the proposed method are compared with wellknown gene/feature selection methods on the basis of performance metric i.e.,classification error rate.A total of 5 gene expression datasets have been used in this study.Different subsets of informative genes are selected by the proposed and all the other methods included in the study,and their efficacy in terms of classification is investigated by using the classifier models such as support vector machine(SVM),Random forest(RF)and k-nearest neighbors(k-NN).The results of the analysis reveal that the proposed method(RSNR)produces minimum error rates than all the other competing feature selection methods in majority of the cases.For further assessment of the method,a detailed simulation study is also conducted.
基金Supported by Department of Biotechnology,Government of India,New Delhi(DBT Project),No.BT/PR 4640/MED/30/716/2012
文摘AIM To investigate the role of genetic variants of angiotensin converting enzyme(ACE) and angiotensinogen(AGT) genes in the antiproteinuric efficacy of ACE inhibitor therapy in diabetic nephropathy(DN) patients.METHODS In the present study, 270 type 2 diabetes mellitus patients with nephropathy were enrolled and treated with ACE inhibitor(ramipril) and followed at 6 mo for renal function and albumin excretion by estimating serum creatinine, end stage renal disease, and albumin/creatinine ratio(ACR) in urine. Genotyping of ACE I/D and AGT M235 T polymorphisms were performed by using primer specific polymerase chain reaction(PCR) and PCR-RFLP techniques, respectively. RESULTS Forty-eight percent of DN patients(responders) benefited with respect to proteinuria from ACE inhibitor therapy at 6 mo follow-up. A significant reduction in ACR was observed after 6 mo treatment with ACE inhibitor irrespective of whether DN patients were micro-albuminuric(≥ 30 and < 300 mg/g creatinine) or macro-albuminuric(≥ 300 mg/g creatinine) at the time of enrollment. However, macro-albuminuric patients(55%) showed better response to therapy. A reduction in urinary ACR was found independent of genotypes of ACE I/D and AGT M235 T polymorphisms although macro-albuminuric patients having TT genotype showed statistically insignificant increased response(72%). CONCLUSION ACE inhibitor therapy reduced urinary ACR by ≥ 30% in 50% of DN patients and the response is independent of ACE I/D and AGT M235 T polymorphisms.
文摘In this paper, based on binomial formula, a law of genetic inheritance is proposed, and it would have certain reference value for future genetic inheritance research. Besides, a recursive algorithm for recessive inheritance is also proposed. Given any two of the male parent, female parent and filial generation, the genomic information of the third one can be calculated uniquely.
基金supported by China Agriculture Research System (CARS-49)Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding (ZF1206)
文摘Palm oil is of great potential as one of the sustainable alternatives to fish oil(FO) in aquafeeds.In this present study,five isonitrogenous diets(32% crude protein) with elevated palm oil levels of 0%,2%,4%,6% and 8% were used during an 8-week feeding trial to evaluate its effects on RNA/DNA ratio and lipoprotein lipase(LPL) and Myo D m RNA expressions in muscle of Oreochromis niloticus.The results showed that RNA,DNA content as well as ratio of RNA to DNA were significantly affected(P < 0.05),in each case the highest was recorded in fish group subjected to 6% palm oil level.There was a strong positive correlation between nucleic acid concentration(RNA concentration and RNA:DNA ratio) and specific growth rate(SGR),protein efficiency ratio(PER),while a negative correlation existed between nucleic acid concentration(RNA concentration and RNA:DNA ratio) and feed conversion ratio(FCR).The m RNA expressions of LPL and Myo D in muscle were not significantly affected by the different palm oil levels,although the highest expression was observed in fish fed with 6% palm oil level.There also existed a strong positive correlation between the m RNA expression of LPL,Myo D and SGR,PER,while their correlation with FCR was negative.In conclusion,elevated palm oil affected the RNA,DNA concentration as well as RNA/DNA ratio significantly,although the m RNA expression of LPL and Myo D were not affected significantly by elevated palm oil levels.
基金the National Natural Science Foundation of China(No. 60371034)the Scientific Research Foundation of Third Military Medical University(2007XG20)
文摘Objective： To discuss strategies and methods of normalization on how to deal with and analyze data for different chips with the combination of statistics, mathematics and bioinformatics in order to find significant difference genes. Methods： With Excel and SPSS software, high or low density chips were analyzed through total intensity normalization （TIN） and locally weighted linear regression normalization （LWLRN）. Results： These methods effectively reduced systemic errors and made data more comparable and reliable. Conclusion： These methods can search the genes of significant difference, although normalization methods are being developed and need to be improved further. Great breakthrough will be obtained in microarray data normalization analysis and transformation with the development of non-linear technology, software and hardware of computer.
文摘Objective:To study the value of serum pepsinogenⅠ andⅡ ratio (PGR) for evaluating the malignant biological behaviors in gastric cancer lesions.Methods:The patients with gastric cancer who underwent radical surgery in 363 Hospital between July 2015 and February 2018 were selected as the gastric cancer group in the study, and the volunteers who had physical examination in 363 Hospital during the same period were selected as the control group. Serum was collected to measure pepsinogenⅠ and pepsinogenⅡ and then calculate the PGR;gastric cancer lesions were collected to measure the expression of oncogenes and angiogenesis genes.Results:Serum PGR level of the gastric cancer group was significantly lower than that of the control group, serum PGR level of the patients with moderately-highly differentiated gastric cancer was significantly higher than that of the patients with lowly differentiated gastric cancer, serum PGR level of the patients with TNM stage III gastric cancer was significantly lower than that of the patients with TNM stage I+II gastric cancer, and serum PGR level of the gastric cancer patients with lymph node metastasis was significantly lower than that of the gastric cancer patients without lymph node metastasis;PGR=3.8 was taken as the cutoff point, and p53 and TXNIP mRNA expression in the lesions of the gastric cancer patients with PGR < 3.8 were significantly lower than those of the gastric cancer patients with PGR > 3.8 while Bcl-2,β-catenin, Survivin, COX-2, HIF-1α, VEGF, c-Met and CNPY2 mRNA expression were significantly higher than those of the gastric cancer patients with PGR > 3.8.Conclusion:The decrease of PGR in serum of patients with gastric cancer is valuable for evaluating the pathological process and malignant biological behaviors.
文摘Objective:To study the correlation between the ultrasonic elastic strain ratio of thyroid cancer and the malignant biological characteristics of cancer cells in the lesions.Methods:A total of 90 patients with thyroid cancer who accepted surgical treatment in our hospital between March 2015 and September 2016 were selected as the observation group, and 50 patients who received surgery in our hospital during the same period and were with clear pathological diagnosis of thyroid adenoma were selected as the control group. According to the median of ultrasonic elastic strain ratio, the observing group were further divided into high elastic strain ratio group and low elastic strain ratio group (n=45). RT-PCR was used to determine the mRNA expression of proliferation genes, invasion genes and autophagy genes in lesions of all groups of patients.Results: The mean elastic strain ratio of observation group was significantly lower than that of control group. Proliferation gene FOXA1 mRNA expression in lesions of low elastic strain ratio group and high elastic strain ratio group were higher than those of control group while PP4R1 and Smac mRNA expression were lower than those of control group, and as the elastic strain ratio decreased, the FOXA1 mRNA expression increased while PP4R1 and Smac mRNA expression decreased;invasion genes BCORL1, SATB1, Twist1 and CXCR4 mRNA expression in lesions of low elastic strain ratio group and high elastic strain ratio group were higher than those of control group, and as the elastic strain ratio decreased, the BCORL1, SATB1, Twist1 and CXCR4 mRNA expression increased;autophagy genes ARHI, Beclin1, LC3 and PTEN mRNA expression in lesions of low elastic strain ratio group and high elastic strain ratio group were lower than those of control group, and as the elastic strain ratio decreased, the ARHI, Beclin1, LC3 and PTEN mRNA expression decreased.Conclusion:The ultrasonic elastic strain ratio of thyroid cancer is negatively correlated with tumor cell proliferation and invasion activity, and positively correlated with autophagy activity.
基金supported by the National Natural Science Foundation of China(Grant Nos.42077106,42277115,and 42177113)the Key R&D Project of Jiangsu Province(Modern Agriculture,Grant No.BE2022322)the Fundamental Research Funds for the Central Universities(Grant No.YDZX2023023).
文摘As the most abundant living entities in the environment,viruses have been well recognized as crucial members in sustaining biogeochemical cycling.However,the significance of viruses in soil ecosystem multifunctionality remains under-explored.In this study,we used metagenomics and meta-viromics analysis to investigate the role of soil viruses in soil ecosystem functions under heavy,light,and no organochlorine pesticides(OCPs)contamination.In the three types of soil samples collected,lightcontaminated soils supported the highest level of multifunctionality,followed by heavy-contaminated soils and clean soils.Additionally,our results revealed a positive correlation between bacterial community evenness and multifunctionality index(p<0.05).Dominant bacterial species with biodegradation and stress resistance advantages exhibited higher abundance in OCPaffected soils,potentially playing a core functional supporting role.Furthermore,our results indicated that the species richness and diversity of bacteriophages were positively correlated with multifunctionality(p<0.05)in OCP-affected soils.Bacteriophages in OCP-affected soils regulate host metabolism and enhance soil ecosystem multifunctionality by infecting functional bacterial hosts and encoding AMGs related to soil element cycling.Our findings emphasize the potential effect of phages on ecosystem multifunctionality in contaminated soil,suggesting that phages may serve as contributors to soil ecology beyond bacteria and other microorganisms.Therefore,in polluted or constrained soils,further research could potentially translate phage communities and related ecological processes into artificial methods for application in soil pollution remediation or ecological restoration.
基金supported by the National Scientific Foundation of China(No.41672227).
文摘Petroleum hydrocarbons,mainly consisting of n-alkanes and polycyclic aromatic hydrocarbons(PAHs),are considered as priority pollutants and biohazards in the environment,eventually affecting the ecosystem and human health.Though many previous studies have investigated the change of bacterial community and alkane degraders during the degradation of petroleum hydrocarbons,there is still lack of understanding on the impacts of soil alkane contamination level.In the present study,microcosms with different n-alkane contamination(1%,3%and 5%)were set up and our results indicated a complete alkane degradation after 30 and 50 days in 1%-and 3%-alkane treatments,respectively.In all the treatments,alkanes with medium-chain length(C_(11)-C_(14))were preferentially degraded by soil microbes,followed by C27-alkane in 3%and 5%treatments.Alkane contamination level slightly altered soil bacterial community,and the main change was the presence and abundance of dominant alkane degraders.Thermogemmatisporaceae,Gemmataceae and Thermodesulfovibrionaceae were highly related to the degradation of C_(14)-and C_(27)-alkanes in 5%treatment,but linked to alkanes with medium-chain(C11-C18)in 1%treatment and C21-alkane in 3%treatment,respectively.Additionally,we compared the abundance of three alkane-monooxygenase genes,e.g.,alk_A,alk_P and alk_R.The abundance of alk_R gene was highest in soils,and alk_P gene was more correlated with alkane degradation efficiency,especially in 5%treatment.Our results suggested that alkane contamination level showed non-negligible effects on soil bacterial communities to some extents,and particularly shaped alkane degraders and degrading genes significantly.This study provides a better understanding on the response of alkane degraders and bacterial communities to soil alkane concentrations,which affects their biodegradation process.
