Genetic variability and trait association analysis in linseed(Linum usitatissimum L.)for yield and related traits

Diversity information mining about a crop for different attributes is an essential step for effective breeding programs.The present investigation evaluates the quantum of genetic variability and determines the relationship among the important agro-economic traits based on two years of phenotypic data of 210 accessions of linseed.The traits,capsule weight per plant,capsule per plant,husk weight per plant,and seed weight per plant exhibited comparatively higher genetic coefficient of variation(GCV)and phenotypic coefficient of variation(PCV).In contrast,oil content and seed per capsule exhibited a lower value.The high magnitude of broad sense heritability was observed for all traits except seeds per capsule and husk weight per plant.The trait,capsules per plant,plant height,and days to 50%flowering showed high genetic advance coupled with high heritability.Hierarchical cluster analysis grouped 210 accessions into six distinct clusters.Out of 210,144(68.57%)accessions were grouped into three clusters(I,II,and III),in which cluster-III was the largest,containing 64 accessions followed by cluster II and cluster-I.The highest inter-cluster distance was observed between clusters-I and V(127.85),while the lowest was between clusters-II and IV(27.09).The positive correlation of capsule weight per plant with the seed weight per plant and a negative correlation with the days to 50%flowering indicates that high yielding linseed varieties with early flowering/maturity could be developed through direct and indirect selection.Further,seed yield and oil content could be enhanced together as indicated by ghe positive association among these two important traits.In this study,high yielding accessions with moderate to high oil content such as GP36,GP31,GP14,GP54,GP26,GP24,GP34,GP21,GP37 and GP27 and early flowering(less than 70 days)accessions such as GP2,GP26,GP27,CG33,CG44,CG42,CG132,and CG31 identified as potential genetic materials that could be exploited for developing early maturing varieties with high yield.In addition,information’s on various genetic parameters will help breeders to devise suitable breeding methodology for linseed genetic improvement for targeted traits.

Estimation of Variability, Heritability and Genetic Advance for Phenological, Physiological and Yield Contributing Attributes in Wheat Genotypes under Heat Stress Condition

The investigation was carried out in focusing the genetic variability for different traits of wheat influenced by heat tolerance mechanism to find out relationships among phenological, physiological and yield contributing traits. Spring wheat cultivar of 25 genotypes were selected and cultivated under late sowing condition at the Regional Wheat Research Institute, Shympur, Rajshahi, Bangladesh from December, 2016 to April, 2017. Significant variability among the genotypes exposed for different traits related to heat tolerance. Results showed that the genotypes G24, G10, G01, G13, G16, G25 and G14 ranked as better category considering maximum number of traits in mean performance indicating their tolerance to heat stress under late sowing condition. Phenotypic variances (σg2) of all traits were greater than those of genotypic variances (σg2). The same trends were also found in their co-efficient of variances. The phenotypic co-efficient of variances (PCV) of all traits were greater compare to those of genotypic co-efficient of variances (GCV) and their values were closer to each other. The heading days (HD), canopy temperature at vegetative stage (CTvg), canopy temperature at grain filling stage (CTgf), biomass, plant height (PH), spike/m2 (SPM), spikelet/spike (SPS), grain/ spike (GPS), thousand grain weight (TGW) and yield exhibited higher heritability (hb2) estimated under irrigated late sowing (ILS) condition. Under the same ILS condition SPAD, SPM, SPS, GPS, TGW and yield showed moderate to high genetic advance (GA) obtained through computing their mean percentage (%) and the rest traits HD, maturity days (MD), CTvg, CTgf, biomass, PH and harvest index (HI) exposed smaller genetic advance (% mean). The co-efficient of variation (CV%) of all attributes in all genotypes were significantly lower (1.36 - 6.96). Both heritability and genetic advance were found lower for MD, SPAD and HI indicated their non additive genetic effects for which these traits might not be recommended for selection. However, spike/m2, spikelet/spike, grain/spike, thousand grain weight and yield belonged to higher heritability and high to moderate genetic advance in mean percentage (%) along with wide genetic variation and lower environmental influence in heat stress situation indicated the most likely heritability due to the effects of additive genes that might be suggested as effective process of selection for these traits in heat stress condition.

Genetic variability,heritability and genetic advance in linseed(Linum usitatissimum L)genotypes for seed yield and other agronomic traits

The experiment was conducted using simple lattice design with two replication and the trails was totally consisted fifty six genotypes.Data on seed yield and other Agronomic traits were used to estimate the genetic variability parameters,heritability and genetic advance(GA).Analysis of variance revealed highly significant and significant difference for all studied traits.Evaluated characters were exhibited different levels of variability,heritability and genetic advance among the studied genotypes.Low to high phenotypic coefficient of variation(PCV)and genotypic coefficient of variation(GCV)were recorded.The highest GCV and PVC values were found particularly for lodging percent(76.65%and 90.63%),harvest index(42.26%and 47.92%)yield per hectare(41.23%and 48.19%)and number of capsule per branch(30.81%and 37.25%)respectively,whereas low GCV and PCV(8.27%and 9.73%respectively)manifested for days to maturate.The highest broad sense heritability value manifested for harvest index(77.78%)followed by seed yield per hectare(73.21%),while lowest heritability(3.78%)revealed only for seed per capsule.In present study low to moderate genetic advance were manifested and high heritability and genetic advance as percentage of mean(>50)was recorded for lodging percentage,number of capsule per branch,seed yield per hectare and harvest index,indicating predominance of additive gene action for these characters.Therefore the result of this study suggests existence of variability for seed yield and other agronomic traits in these linseed genotypes,which should be exploited in future breeding.

Genetic evaluation of twenty seed sources of Asparagus racemosus

A field trial of 20 seed sources of Asparagus racemosus was conducted at the Forest Research Institute, Dehradun, Uttarakhand, India to evaluate their performance of different economic traits. Genotypic variance, phenotypic variance, genotypic coefficient of variance （GCV） and phenotypic coefficient of variance （PCV） for number of shoots, shoot height, shoot weight, number of roots, root length, root diameter and root weight were calculated. Maximum genotypic and phenotypic variance was observed in shoot height among the shoot - related traits and root length among the root - related traits. For the shoot height, genotypic variance, phenotypic variance, genotypic coefficient of variance, phenotypic coefficient of variance were 231.80, 3924.80, 61.26 and 1037.32, respectively, where those of the root length were 9.55, 16.80, 23.46 and 41.27, respectively. The maximum genetic advance and genetic gain were obtained for shoot height among the shoot-related traits and root length among the root-related traits. Index values were developed for all the seed sources based on the four most important traits, and Panthnagar （Uttrakhand）, Jodhpur （Rajasthan）, Dehradun （Uttarakhand）, Chandigarh （Punjab）, Jammu （Jammu and Kashmir） and Solan （Himachal Pradesh）, were promising seed sources for root production.

Agro-Morphological Characterization and Genetic Dissection of Linseed(Linum usitatissimum L.)Genotypes

Linseed is a multipurpose crop and the crop needs further improvement to increase production and yield due to its high value and demand.This study aimed to assess the extent and pattern of genetic variability of forty linseed genotypes based on diverse agro–morphological and yield attributes.The field experiment was conducted following a Randomized Complete Block Design with three replications.Linseed germplasm showed a wide range of phenotypic expression,genetic variability and heritability for 30 studied traits.A low to high phenotypic coeffi-cient of variation(PCV)and genotypic coefficient of variation(GCV)were observed.The lowest genotypic(σ2 g)and phenotypic variances(σ2 p)were found in capsule diameter(CD),length of calyx(LC),capsule length(CL),seed length(SL),and seed breadth(SB).High broad-sense heritability(h2 b)with high genetic advance as a percentage of mean(GAM)were observed in days to germination started(DGS),days to 80%emergence(DE),plant height at 28 and 40 DAS,number of flowers(NFPP),filled capsules(NFCPP)and yield per plant(YPP)indicating additive gene action exists for these characters.Hierarchical cluster analysis separated 40 genotypes into five clusters,where Clusters I to V assembled with 13,4,4,5 and 14 genotypes,respectively.Considering yield and yield attributes,Cluster-IV(G3,G4,G6,G10 and G31)genotypes showed promising while,Cluster-II(G2,G16,G35,G36)and Cluster-III(G1,G33,G39 and G40)genotypes were dominant on plant morphological traits.Based on principal component analysis(PCA),few characters such as YPP,NFPP,NFCPP,days to first flowering and capsule formation,early emergence,days to branch initiation and plant heights at different growth stages revealed important and effective traits for consideration in the selection of linseed breeding programs.

Breeding Potential of Some Exotic Tomato Lines:A Combined Study of Morphological Variability,Genetic Divergence,and Association of Traits

Tomato(Solanum lycopersicum L.)is called‘the poor man’s orange’due to its low price and improved nutritional values.An experiment was conducted to study the breeding potential of some exotic tomato lines by assessing various qualitative and quantitative traits conferring yield and quality attributes.Among the qualitative traits,greater variability was observed for growth type,stem hairiness,and fruit shape and size.A determinate growth habit was observed in the genotype AVTO9802 while the genotype AVTO0102 produced yellow color fruits.A significant(p≤0.01)variation was also observed for the studied quantitative traits.Based on yield and traits attributed to yield,the genotypes AVTO0314,GPB0107,GPB0120 and AVTO9802 were selected as promising genotypes.The differences between the genotypic and phenotypic coefficients of variation(GCV and PCV)of the studied quantitative traits were very low.This suggests that the apparent variation was mainly due to the genotypes.The higher GCVand PCV values were observed for the number of primary branches plant^(−1)(NPB),number of fruits cluster^(−1)(NFC),individual fruit weight(IFW)and total soluble solids(TSS).High heritability was recorded for all quantitative traits in a broad sense.However,the individual fruit diameter showed the highest heritability(99.56).The highest(102.75)genetic advance(GA)was observed for the number of fruits plant^(−1)(NFP).High heritability coupled with high GA as percentage of mean were recorded for the traits NFP,NFC,fruit yield plant^(−1)(FYP)and IFW.FYP showed a significant positive correlation with NFC(0.714^(***))and a negative correlation with days to the first harvest(−0.539^(***))and plant height(−0.492**).Principal component analysis revealed that the first four components explained 78.5%of the total variation among the genotypes.Thus,the promising genotypes(AVTO0314,GPB0107,GPB0120,AVTO9802 and AVTO0102)isolated from this study can be used for developing high-yielding and high-quality tomato varieties.

Variability and divergence in Pongamia pinnata for further use in tree improvement

A total of 24 candidate plus trees（CPTs） of Pongamia pinnata（L.） Pierre.were selected to elucidate their variation and diversity based on thirteen quantitative traits（4 pod traits,6 seed traits of parent trees and 3 progeny traits） at Forest Research Centre,Institute of Forest Productivity-Mandar,Ranchi district during 2005-2007.The results show that,CPT-19 had maximum for seven traits viz,pod length（65.6 mm）,100-pod weight（542.4 g）,seed 2D（two dimension） area（351.2 mm2）,seed length（27.9 mm）,seed breadth（17.4 mm）,100-seed weight（217.9 g） and plant height（164.3 cm）.The traits,100-pod weight and 100-seed weight had a high heritability（98.4%,96.9%） accompanied with high genetic advance（46.0%,34.9%）.There is a positive significant correlation between 100-pod weight and 100-seed weight traits at both genotypic and phenotypic levels with plant height,collar diameter and volume index at 30 MAS（months after sowing）.Volume index expressed a moderate heritability（47.4%） accompanied with high genetic advance（48.4%）,indicating that the character is governed by additive gene effects.In divergence study,24 accessions were grouped into 6 clusters on the basis of non-hierarchical euclidian cluster analysis.The genotypes in cluster IV（CPT-5,CPT-6,CPT-7,CPT-12,CPT-16,CPT18,CPT-22） and cluster III（CPT-4,CPT-8,CPT-9,CPT-20,CPT-21） were most heterogeneous and can be best used within group hybridization.The wide diversity exists between the cluster V and II,followed by cluster II and I and crosses between CPTs of these clusters may result in substantial segregates.It is revealed that the existence of substantial variation and diversity can be utilized for genetic resource conservation and further tree improvement programmers of the species.

Variation,inheritance and correlation of growth characteristics of Populus deltoides Bartr.at various ages in the central-plain region of Punjab,India

The growth performance of twenty poplar （Populus deltoides Bartr.） clones, planted in the central-plain region of Punjab in north-western India, was evaluated using a randomized block design with three replications and plot size of four plants. Significant differences among clones were observed for diameter at breast height （DBH）, height and volume per tree at age five, six and eight years. Clone L-48/89 recorded significantly superior volume than the control （G-48） at all ages, with superiority of 51.4, 43.9 and 48.5 per cent at age five, six and eight years, respectively. The phenotypic and genotypic coefficients of variation were the highest for volume （26.55-34.66% and 15.84-26.00%） and the minimum for tree height （8.43-12.13% and 4.71-7.59%）. The broad sense heritability was relatively higher for DBH （0.42-0.55） and genetic advance as per cent of mean was highest for volume （19.48-40.18）. All genetic parameters increased with age. All traits showed significant positive correlation with each other.

DYNAMIC ADVANCED PLANNING AND SCHEDULING WITH FROZEN INTERVAL FOR NEW ORDERS

A dynamic advanced planning and scheduling （DAPS） problem is addressed where new orders arrive on a continuous basis. A periodic policy with frozen interval is adopted to increase stability on the shop floor. A genetic algorithm is developed to find a schedule at each rescheduling point for both original orders and new orders that both production idle time and penalties on tardiness and earliness of orders are minimized. The proposed methodology is tested on a small example to illustrate the effect of the frozen interval. The results indicate that the suggested approach can improve the schedule stability while retaining efficiency.

Variability in growth characteristics for different genotypes of Eucalyptus tereticornis (SM.)

Eighteen clones of Eucalyptus tereticornis （Sm.） were evaluated for three years by adopting randomized block design for various growth parameters at Hoshiarpur, Punjab, India and compared with two checks. Significant variations were recorded for height, diameter at breast height （DBH） and clear bole height （CBH）. The broad sense heritability was low to moderate for both height and CBH. The genetic gain for height and CBH increased substantially pe se with the increase in age of trees. The average genetic gain for thres years was recorded maximum for height （159.60%） followed by DBH （110.97%） and CBH （70.34%）. Clone 17 attained maximum DBH over other genotypes for second and third year followed by clones 14 and 11. Clone 5 showed an upward trend for DBH and maintained its superiority for CBH as the age of the tree increased. Similarly, clone 11 changed its ranking from 9th to 8th to 3rd for DBH and from 9th to 4th to 2nd for CBH, respectively for the age of one, two and three years. Nonetheless, clones 6 and 10 per- formed poorly for all the characters studied. Clones 17, 14 and 5 were found to be the most promising clones for commercial deployment.

Identification of diverse genotypes with high oil content in Madhuca latifolia for further use in tree improvement

Madhuca latifolia Macb.commonly known as Indian butter tree,is an open-pollinated plant.Improvement in seed and oil yields depends on the progress in the desired characters in the base material and the genetic variability available in the collected germplasm.We evaluated 23 genotypes of M.latifolia to understand genetic variability,character association and divergence in seed traits and oil content for use in breeding programs.Variation was recorded in seed length（27.3–38.6 mm）,seed breadth（15.6–19.1 mm）,two dimensional（2D） surface area（328.3–495.4 mm2）,100 seed weight（216.8–285.3 g）,acid value（13.4–25.8 mg KOH/g）,iodine number（62.4–78.6） and oil content（37.8–51.0 %）.High estimates of genotypic coefficient of variation,broad senseheritability and genetic gain were observed for seed oil content.Variability studies for seed traits revealed that genotype CPT-16 had the highest 100-seed weight（281.5 g） and oil content（51 %）.Highly significant genotypic and phenotypic correlations were observed.The100-seed weight was positively and significantly correlated with oil content at both phenotypic（r = 0.57） and genotypic（r = 0.60） levels.Cluster analysis of the scores of the first three principle components（80.83 %） resulted in four clusters,consisting of 4,7,3 and 9 genotypes in the first,second,third and fourth clusters,respectively.Cluster 3was distinguished from others based on significantly higher means for most seed traits except seed breadth,acid value,iodine number and oil content.Cluster 1 appeared more divergent as it had significantly higher means for acid value and iodine number.A comparative assessment of means of the four clusters for 100-seed weight and oil content suggested that cluster 3 would be useful for higher 100-seed weight and oil content.Hence these genotypes,CPT-3,CPT-6 and CPT-15 in cluster 3 can be used for direct selection and utilization in breeding programs.

Inheritance of Angular Leaf Spot [Phaeoisariopsis griseola （Sacc.） Ferr] Resistance in Common Bean （Phaseolus vulgaris L,） Population Developed from Kablanketi x Mexico 54

The genetic resistance to angular leaf spot （ALS） caused by Phaeoisariopsis griseola in the common bean cultivar Mexico 54 was investigated on disease reactions in parental, F1, F2 and backcross generations derived from crosses between a resistant cultivar Mexico 54 and a susceptible cultivar Kablanketi under screen house conditions. The heritability （h2） estimate was as high as 0.719 indicating a successful transfer of ALS resistance among progenies and thus selection can be performed in early generation. High heritability coupled with high expected genetic advance of 39.5% is considered to be more useful in predicting the outcome of selecting the best individuals. Chi-square values were computed to determine whether the observed ratios for disease reactions deviated from expected Mendelian ratios for a single, dominant gene controlling resistance to angular leaf spot in common bean. Based on the resistance of the F2, and the backcross generation to the resistant parent, a 3 resistant： 1 susceptible segregation ratio in the F2 and a 1 resistant： 1 susceptible segregation ratio in the backcross generation to the susceptible parent was obtained implying that resistance to the isolate ofPhaeoisariopsis griseola is governed by a single, dominant gene.

Heritability and Correlation among Sugarcane (Saccharum spp.) Yield and Some Agronomic and Sugar Quality Traits in Ethiopia

To assess broad sense heritability and phenotypic and genetic correlations among sugarcane yield components, an experiment was conducted at Wonji and Metehara Sugar Estates of Sugar Corporation of Ethiopia during 2012/2013. High broad sense heritability (h2) was detected for stalk diameter (0.730), single cane weight (0.672), millable cane number (0.624), stalk height (0.624) and pol % (0.608), indicating that these traits could be selected for easily. Expected genetic gain of the yield components was moderate to high. All traits had low to high genetic correlations (rg = -0.005 to 0.884) with cane yield and (rg = 0.027 to 0.999) with sugar yield. On average genetic correlations were higher than phenotypic correlations. High Genotypic Coefficient of Variation (GCV), broad sense heritability and expected genetic advance were recorded for stalk diameter, single cane weight and millable cane number. A selection strategy based on these traits could lead to improvement in cane and sugar yield.

Genetic counseling, prenatal screening and diagnosis of Down syndrome in the second trimester in women of advanced maternal age： a prospective study

Background The incidence of autosomal trisomy in livebirths is strongly dependent on maternal age. Special consideration is given to the provision of prenatal screening and cytogenetic testing to women of advanced maternal age （AMA）. The aim of this study was to evaluate the effectiveness of second trimester prenatal screening and amniocentesis for Down syndrome （DS） and compare the trends of choice of screening and amniocentesis among AMA women. Methods A total of 5404 AMA patients with natural singleton pregnancy were recruited for this prospective study from January 2008 to December 2010. The gestational weeks were from 15 weeks to 20~6 weeks. The patients referred were grouped into a screening group （2107 cases） and an amniocentesis group （3297 cases） by their own decision. The prevalence of DS was compared between the two groups by chi-square test. Choice rates for each maternal age with trends were compared by regression analysis. Results There were 18 cases of fetal DS detected in the screening group with a prevalence of 8.54%o （18/2107）. Twenty- five cases of fetal DS were diagnosed in the amniocentesis group with a prevalence of 7,58%0 （25/3297）. No statistical difference was observed in the prevalence of DS between the screening and amniocentesis group （P=0.928）. The invasive testing rate for DS in the amniocentesis group was 5.54 times higher than that of the screening group （1/131.88 vs. 1/23.78）. With the increase of the maternal age, the choice of amniocentesis increased while the choice of the screening showed an opposite trend. The choice of the AMA women between the screening and amniocentesis was significantly age relevant （P=0.012）. Conclusions The second trimester serum screening age alone to screen for DS. We suggest educating screening and amniocentesis options. in combination with maternal age was more effective than maternal the patients by recommending AMA women be informed of both

Multi-objective optimization strategy for distribution network considering V2Genabled electric vehicles in building integrated energy system

Based on the large-scale penetration of electric vehicles(EV)into the building cluster,a multi-objective optimal strategy considering the coordinated dispatch of EV is proposed,for improving the safe and economical operation problems of distribution network.The system power loss and node voltage excursion can be effectively reduced,by taking measures of time-of-use(TOU)price mechanism bonded with the reactive compensation of energy storage devices.Firstly,the coordinate charging/discharging load model for EV has been established,to obtain a narrowed gap between load peak and valley.Next,a multi-objective optimization model of the distribution grid is also defined,and the active power loss and node voltage fluctuation are chosen to be the objective function.For improving the efficiency of optimization process,an advanced genetic algorithm associated with elite preservation policy is used.Finally,reactive compensation capacity supplied by capacitor banks is dynamically determined according to the varying building loads.The proposed strategy is demonstrated on the IEEE 33-node test case,and the simulation results show that the power supply pressure can be obviously relieved by introducing the coordinated charging/discharging behavior of EV;in the meantime,via reasonable planning of the compensation capacitor,the remarkably lower active power loss and voltage excursion can be realized,ensuring the safe and economical operation of the distribution system.