Background: The suitability of micro-computed tomography (CT) for soft tissue applications has been well documented. Although the application of micro-CT to the three dimensional (3D) structure of the tongue muscle ha...Background: The suitability of micro-computed tomography (CT) for soft tissue applications has been well documented. Although the application of micro-CT to the three dimensional (3D) structure of the tongue muscle has been reported, a 3D rendering and/or a schematic view of the tongue muscle has yet to be published. Material and Method: First, muse tongues were fixed and decalcified, and then the vertical muscle (Ve), the transverse muscle (Tr), and/or the genioglossus muscle of the mouse tongue (Ge) were analyzed using micro-CT and are shown in this report in rendered images and pattern diagrams. Results: 1) The Tr is classified into three parts: the first part extends from the middle to the apical part of the tongue;the second part is strongly connected to the superior longitudinal muscles of the tongue (Lo);the third part fans out from the middle to the root of the tongue. 2) The Ve is classified into two main groups: the first group joins the dorsal and the lateral parts of the tongue;the second group joins the dorsal part and the floor of the tongue. 3) Ge is classified into four parts: three parts comprise the Ge apical and middle parts of the tongue, with one part in the inferior longitudinal muscles of the tongue, one joining the lingual septum of the tongue (LS), and the other joining the sub-surface of the dorsal part of the Lo. The remaining Ge exits in a fan-like manner through the root of the tongue and then joins the Tr.展开更多
目的研究低频复合生理频率慢性电刺激对低压缺氧兔颏舌肌肌球蛋白重链(myosin heavy chain,MHC)亚型表达的影响。方法成年日本大白兔24只,雌雄不拘,体质量(2.3±0.1)kg,按随机数字表法分为4组(n=6)。A:正常对照组,B:慢性低压缺氧组,...目的研究低频复合生理频率慢性电刺激对低压缺氧兔颏舌肌肌球蛋白重链(myosin heavy chain,MHC)亚型表达的影响。方法成年日本大白兔24只,雌雄不拘,体质量(2.3±0.1)kg,按随机数字表法分为4组(n=6)。A:正常对照组,B:慢性低压缺氧组,C:10Hz电刺激组,D:10+40Hz电刺激组。B、C、D组兔置于慢性低压缺氧环境中饲养后,分别给予C组兔颏舌肌低频(10Hz)电刺激,D组兔颏舌肌低频复合生理频率(10+40Hz)电刺激,B组兔不予电刺激。采用SDS-聚丙烯酰胺凝胶电泳及半定量反转录聚合酶链式反应(RT-PCR)法,分析4组颏舌肌MHC各亚型表达变化。结果①与正常对照组比较,慢性低压缺氧组颏舌肌MHCⅠ亚型比例减少[(0.69±0.07)%vs(17.48±4.00)%,P<0.05],MHCⅡa亚型比例增加[(79.89±5.09)%vs(73.33±4.17)%,P<0.05];MHCⅠ亚型mRNA表达量下降[(0.69±0.07)vs(1.05±0.06),P<0.05]。②与慢性低压缺氧组比较,10Hz电刺激组颏舌肌MHCⅠ亚型比例[(18.78±3.49)%vs(11.37±4.27)%,P<0.05]及10+40Hz电刺激组颏舌肌MHCⅠ亚型比例[(23.40±4.65)%vs(11.37±4.27)%,P<0.05]均增加,而10+40Hz电刺激组颏舌肌MHCⅡa亚型比例[(67.90±5.66)%vs(79.89±5.09)%,P<0.05]减少;与慢性低压缺氧组比较,10Hz电刺激组颏舌肌MHCⅠ亚型mRNA表达量[(0.79±0.07)vs(0.69±0.07),P<0.05]及10+40Hz电刺激组颏舌肌MHCⅠ亚型mRNA表达量[(0.97±0.08)vs(0.69±0.07),P<0.05]均增高,而MHCⅡa亚型mRNA表达量差异不显著。结论慢性电刺激后低压缺氧兔颏舌肌MHC呈现Ⅱa向Ⅰ型转变,即"由快向慢"转变。其中低频复合生理频率电刺激组变化的趋势更加明显。展开更多
文摘Background: The suitability of micro-computed tomography (CT) for soft tissue applications has been well documented. Although the application of micro-CT to the three dimensional (3D) structure of the tongue muscle has been reported, a 3D rendering and/or a schematic view of the tongue muscle has yet to be published. Material and Method: First, muse tongues were fixed and decalcified, and then the vertical muscle (Ve), the transverse muscle (Tr), and/or the genioglossus muscle of the mouse tongue (Ge) were analyzed using micro-CT and are shown in this report in rendered images and pattern diagrams. Results: 1) The Tr is classified into three parts: the first part extends from the middle to the apical part of the tongue;the second part is strongly connected to the superior longitudinal muscles of the tongue (Lo);the third part fans out from the middle to the root of the tongue. 2) The Ve is classified into two main groups: the first group joins the dorsal and the lateral parts of the tongue;the second group joins the dorsal part and the floor of the tongue. 3) Ge is classified into four parts: three parts comprise the Ge apical and middle parts of the tongue, with one part in the inferior longitudinal muscles of the tongue, one joining the lingual septum of the tongue (LS), and the other joining the sub-surface of the dorsal part of the Lo. The remaining Ge exits in a fan-like manner through the root of the tongue and then joins the Tr.
文摘目的研究低频复合生理频率慢性电刺激对低压缺氧兔颏舌肌肌球蛋白重链(myosin heavy chain,MHC)亚型表达的影响。方法成年日本大白兔24只,雌雄不拘,体质量(2.3±0.1)kg,按随机数字表法分为4组(n=6)。A:正常对照组,B:慢性低压缺氧组,C:10Hz电刺激组,D:10+40Hz电刺激组。B、C、D组兔置于慢性低压缺氧环境中饲养后,分别给予C组兔颏舌肌低频(10Hz)电刺激,D组兔颏舌肌低频复合生理频率(10+40Hz)电刺激,B组兔不予电刺激。采用SDS-聚丙烯酰胺凝胶电泳及半定量反转录聚合酶链式反应(RT-PCR)法,分析4组颏舌肌MHC各亚型表达变化。结果①与正常对照组比较,慢性低压缺氧组颏舌肌MHCⅠ亚型比例减少[(0.69±0.07)%vs(17.48±4.00)%,P<0.05],MHCⅡa亚型比例增加[(79.89±5.09)%vs(73.33±4.17)%,P<0.05];MHCⅠ亚型mRNA表达量下降[(0.69±0.07)vs(1.05±0.06),P<0.05]。②与慢性低压缺氧组比较,10Hz电刺激组颏舌肌MHCⅠ亚型比例[(18.78±3.49)%vs(11.37±4.27)%,P<0.05]及10+40Hz电刺激组颏舌肌MHCⅠ亚型比例[(23.40±4.65)%vs(11.37±4.27)%,P<0.05]均增加,而10+40Hz电刺激组颏舌肌MHCⅡa亚型比例[(67.90±5.66)%vs(79.89±5.09)%,P<0.05]减少;与慢性低压缺氧组比较,10Hz电刺激组颏舌肌MHCⅠ亚型mRNA表达量[(0.79±0.07)vs(0.69±0.07),P<0.05]及10+40Hz电刺激组颏舌肌MHCⅠ亚型mRNA表达量[(0.97±0.08)vs(0.69±0.07),P<0.05]均增高,而MHCⅡa亚型mRNA表达量差异不显著。结论慢性电刺激后低压缺氧兔颏舌肌MHC呈现Ⅱa向Ⅰ型转变,即"由快向慢"转变。其中低频复合生理频率电刺激组变化的趋势更加明显。
