Pharmacological, laser scanning confocal microscopic （LSCM）, and spectrophotographic approaches were used to study the roles of hydrogen sulfide （H2S） and nitric oxide （NO） in signaling transduction of stomatal ...Pharmacological, laser scanning confocal microscopic （LSCM）, and spectrophotographic approaches were used to study the roles of hydrogen sulfide （H2S） and nitric oxide （NO） in signaling transduction of stomatal movement in response to ethylene in Viciafaba L. Ethylene treatment resulted in the dose-dependent stomatal closure under light, and this effect was blocked by the inhibitors of H2S biosynthesis in V. faba L. Additionally, ethylene induces H2S generation and increases L-/D-cysteine desulfhydrase （pyridoxalphosphate-dependent enzyme） activity in leaves of V. faba L. Inhibitors of H2S biosynthesis have no effect on the ethylene-induced stomatal closure, NO accumulation, and nitrate reductase （NR） activity in guard cells or leaves of II. faba L. Moreover, the ethylene-induced increase of H2S levels and L-/D- cysteine desulfhydrase activity declined when NO generation was inhibited. Therefore, we conclude that H2S and NO probably are involved in the signal transduction pathway of ethylene-induced stomatal closure. H2S may represent a novel component downstream of NO in the ethylene-induced stomatal movement in V. faba L.展开更多
To analyze the nutritional composition of faba bean(Vicia faba L.) seed, estimation models were developed for protein, starch, oil, and total polyphenol using near infrared spectroscopy(NIRS). Two hundred and forty-fo...To analyze the nutritional composition of faba bean(Vicia faba L.) seed, estimation models were developed for protein, starch, oil, and total polyphenol using near infrared spectroscopy(NIRS). Two hundred and forty-four samples from twelve producing regions were measured in both milled powder and intact seed forms. Partial least squares(PLS) regression was applied for model development. The model based on ground seed powder was generally superior to that based on the intact seed. The optimal seed powder-based models for protein, starch, and total polyphenol had coefficients of correlation(r2) of 0.97, 0.93 and 0.89, respectively. The relationship between nutrient contents and twelve producing areas was determined by two-step cluster analysis. Three distinct groupings were obtained with region-constituent features, i.e., Group 1 of high oil, Group 2 of high protein, and Group 3 of high starch as well as total polyphenol. The clustering accuracy was 79.5%. Moreover, the nutrition contents were affected by seeding date, longitude, latitude, and altitude of plant location. Cluster analysis revealed that the differences in the seed were strongly influenced by geographical factors.展开更多
Trace elements in four kinds of the Genus Hippophae L pulps were analysed byPIXE. Optimization of the system performance was done by carefully selecting the absorbers, Analytical sensitivities of the PIXE system were ...Trace elements in four kinds of the Genus Hippophae L pulps were analysed byPIXE. Optimization of the system performance was done by carefully selecting the absorbers, Analytical sensitivities of the PIXE system were obtained by means of "external standard method".展开更多
[Objectives] This study was conducted to give play to the yield-increasing potential of new broad bean varieties. [Methods] The correlation analysis and principal component analysis of eight main agronomic traits of 1...[Objectives] This study was conducted to give play to the yield-increasing potential of new broad bean varieties. [Methods] The correlation analysis and principal component analysis of eight main agronomic traits of 15 autumn-sown broad bean varieties in 2017-2018 were carried out. [Results] There were extremely complicated correlations between the agronomic traits. Among the various agronomic traits, the number of pods per plant showed a significant negative correlation from the number of seeds per pod(P<0.05), and significant negative correlations with pod length and 100-grain weight(P<0.01). The number of seeds per pod was significantly positively correlated with pod length(P<0.01). The 100-grain weight of broad beans was significantly positively correlated with the pod length(P<0.01), and was significantly negatively correlated with the number of pods per plant(P<0.01). Three principal components were established by principal component analysis, and the contribution rate of the first principal component was the highest, which was 40.18%. The contribution rates of the second principal component and the third principal component to the total variance were 24.08% and 13.55%, respectively. The score equations of the principal components were derived. [Conclusions] This study provides a theoretical reference for the application and promotion of new varieties of autumn broad beans.展开更多
基金supported by the National Natural Science Foundation of China (30970288 and 31170237)the National Science Foundation of Shandong Province, China(ZR2010CM024)the Foundation of State Key Laboratory of Plant Physiology and Biochemistry, China(SKLPPBKF11001)
文摘Pharmacological, laser scanning confocal microscopic （LSCM）, and spectrophotographic approaches were used to study the roles of hydrogen sulfide （H2S） and nitric oxide （NO） in signaling transduction of stomatal movement in response to ethylene in Viciafaba L. Ethylene treatment resulted in the dose-dependent stomatal closure under light, and this effect was blocked by the inhibitors of H2S biosynthesis in V. faba L. Additionally, ethylene induces H2S generation and increases L-/D-cysteine desulfhydrase （pyridoxalphosphate-dependent enzyme） activity in leaves of V. faba L. Inhibitors of H2S biosynthesis have no effect on the ethylene-induced stomatal closure, NO accumulation, and nitrate reductase （NR） activity in guard cells or leaves of II. faba L. Moreover, the ethylene-induced increase of H2S levels and L-/D- cysteine desulfhydrase activity declined when NO generation was inhibited. Therefore, we conclude that H2S and NO probably are involved in the signal transduction pathway of ethylene-induced stomatal closure. H2S may represent a novel component downstream of NO in the ethylene-induced stomatal movement in V. faba L.
基金financed by the Modern Agro-industry Technology Research System (nycyty-018: Guixing Ren)the National Infrastructure of Crop Germplasm Resources and the Sci & Tech Innovation Program of CAAS
文摘To analyze the nutritional composition of faba bean(Vicia faba L.) seed, estimation models were developed for protein, starch, oil, and total polyphenol using near infrared spectroscopy(NIRS). Two hundred and forty-four samples from twelve producing regions were measured in both milled powder and intact seed forms. Partial least squares(PLS) regression was applied for model development. The model based on ground seed powder was generally superior to that based on the intact seed. The optimal seed powder-based models for protein, starch, and total polyphenol had coefficients of correlation(r2) of 0.97, 0.93 and 0.89, respectively. The relationship between nutrient contents and twelve producing areas was determined by two-step cluster analysis. Three distinct groupings were obtained with region-constituent features, i.e., Group 1 of high oil, Group 2 of high protein, and Group 3 of high starch as well as total polyphenol. The clustering accuracy was 79.5%. Moreover, the nutrition contents were affected by seeding date, longitude, latitude, and altitude of plant location. Cluster analysis revealed that the differences in the seed were strongly influenced by geographical factors.
基金The Project Supported by National Natural Science Foundation of China
文摘Trace elements in four kinds of the Genus Hippophae L pulps were analysed byPIXE. Optimization of the system performance was done by carefully selecting the absorbers, Analytical sensitivities of the PIXE system were obtained by means of "external standard method".
基金Supported by Hubei Agricultural Science and Technology Innovation Center Fund(2016-620-000-001-006)Hubei Agricultural Germplasm Resource Sharing Platform(PTJX2015000008)National Modern Agriculture Industrial Technology System Construction Project(CARS-09)
文摘[Objectives] This study was conducted to give play to the yield-increasing potential of new broad bean varieties. [Methods] The correlation analysis and principal component analysis of eight main agronomic traits of 15 autumn-sown broad bean varieties in 2017-2018 were carried out. [Results] There were extremely complicated correlations between the agronomic traits. Among the various agronomic traits, the number of pods per plant showed a significant negative correlation from the number of seeds per pod(P<0.05), and significant negative correlations with pod length and 100-grain weight(P<0.01). The number of seeds per pod was significantly positively correlated with pod length(P<0.01). The 100-grain weight of broad beans was significantly positively correlated with the pod length(P<0.01), and was significantly negatively correlated with the number of pods per plant(P<0.01). Three principal components were established by principal component analysis, and the contribution rate of the first principal component was the highest, which was 40.18%. The contribution rates of the second principal component and the third principal component to the total variance were 24.08% and 13.55%, respectively. The score equations of the principal components were derived. [Conclusions] This study provides a theoretical reference for the application and promotion of new varieties of autumn broad beans.
