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Is Bat Guano a Reservoir of <i>Geomyces destructans</i>?
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作者 Janez Mulec Elizabeth Covington Julia Walochnik 《Open Journal of Veterinary Medicine》 2013年第2期161-167,共7页
Bat guano from six different karst caves in Slovenia was screened by PCR for the presence of Geomyces destructans [1]. Two identical DNA sequences from guano heaps in two of the caves with recent bat droppings were re... Bat guano from six different karst caves in Slovenia was screened by PCR for the presence of Geomyces destructans [1]. Two identical DNA sequences from guano heaps in two of the caves with recent bat droppings were retrieved. These sequences were closely related to several unidentified Geomyces clones and to pathogenic G. destructans, but definitive identification remained elusive. Despite the presence of Geomyces species, thus far there has been no report of Whitenose syndrome (WNS) symptoms in bats in Slovenia. Overall physical and chemical conditions in guano, particularly in large guano heaps, offer a suitable environment which might serve as a reservoir for pathogenic Geomyces strains and could represent a permanent reservoir for in situ infection of bats. Screening of guano and analysis of more data related to guano ecology could lend clues to control WNS outbreaks and mortality linked to G. destructans. 展开更多
关键词 geomyces destructans BAT GUANO CAVE Slovenia
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Improving the antioxidant activity and enriching salvianolic acids by the fermentation of Salvia miltiorrhizae with Geomyces luteus 被引量:5
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作者 Yun XING Le CAI +4 位作者 Tian-peng YIN Yang CHEN Jing YU Ya-rong WANG Zhong-tao DING 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2016年第5期391-398,共8页
The antioxidant activities and total phenolic content of fermented Salvia miltiorrhiza with fungus Geomyces luteus were investigated. The results revealed that G. luteus fermentation could significantly improve the an... The antioxidant activities and total phenolic content of fermented Salvia miltiorrhiza with fungus Geomyces luteus were investigated. The results revealed that G. luteus fermentation could significantly improve the antioxidant activity and total phenolic content of S. miltiorrhiza. The main antioxidant constituents were characterized by spec- troscopic analysis as salvianolic acids. High-performance liquid chromatography (HPLC) quantification also showed the enhanced content of salvianolic acid B after fermentation. The present study suggests that G. luteus fermentations are effective in the S. miltiorrhiza salvianolic acids' enrichment process. 展开更多
关键词 Salvia miltiorrhiza geomyces luteus Salvianolic acids ANTIOXIDANT FERMENTATION
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低温秸秆降解复合菌系分离及菌株鉴定
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作者 吴清云 萨如拉 +3 位作者 杨恒山 杨陶阳 王涛 雷梓莉 《中国农学通报》 2023年第21期62-67,共6页
为内蒙古冷凉地区秸秆促熟还田提供优良微生物菌剂,需要明确低温秸秆降解复合菌系菌种组成。以15℃实验室条件下能降解玉米秸秆的复合菌系-8号为材料,采用选择性培养基-羧甲基纤维素钠固体培养基分离获得单菌株,并从菌株形态学、生理生... 为内蒙古冷凉地区秸秆促熟还田提供优良微生物菌剂,需要明确低温秸秆降解复合菌系菌种组成。以15℃实验室条件下能降解玉米秸秆的复合菌系-8号为材料,采用选择性培养基-羧甲基纤维素钠固体培养基分离获得单菌株,并从菌株形态学、生理生化特性、细菌16S rRNA、真菌ITS rDNA和?-tubulin基因序列分析方法对其进行鉴定。结果表明,8号菌系用羧甲基纤维素钠固体培养基分离到真菌8-F和细菌8-B。8-F菌落为粉白色、毡绒状、革兰氏阴性,菌丝经常缠绕成绳状,分生孢子梗呈树状或葡萄穗状分枝、直径1.5~2.0μm,分生孢子顶生或间生、倒梨状、大小2.5~4.5×2.0~3.0μm,其ITS rDNA序列与GenBank数据库中数据系统比对可知,与Geomyces sp.具有99%的同源性;根据以上特点鉴定为Geomyces pannorum。8-B菌细胞杆状、革兰氏阴性、不生孢,菌落呈浅黄色、不规则形状,能产10种酶、能利用37种碳源,其16S rDNA序列与Stenotrophomonas humi的同源性为99.71%,根据以上特点鉴定为寡养单胞菌属(Stenotrophomonas sp.)。在低温(15℃)实验室条件下能降解玉米秸秆的复合菌系优势菌种是Geomyces pannorum和Stenotrophomonas maltophilia。 展开更多
关键词 富集 驯化 geomyces pannorum 嗜麦芽寡养单胞菌
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