Bioassay and GC-MS determination indicated that inhibitors of seeds of Fraxinus mandshurica Rupr were distributed mainly in zones of β-inhibitors (including ABA) and of high and low Rf value. The inhibitory activity ...Bioassay and GC-MS determination indicated that inhibitors of seeds of Fraxinus mandshurica Rupr were distributed mainly in zones of β-inhibitors (including ABA) and of high and low Rf value. The inhibitory activity of outer seed coat of the dormant seed (control treatment) was higher than that of seeds with only the inner coat attached e.g. ABA was 114 and 54ng/g fresh weight respectively. The inhibitory effect of No. 12 zone was even higher than ABA. The inhibitory activity of all the zones decreased after stratification (St) and burying (AO) treatment, especially that of the outer seed coat, whose ABA content decreased to 7(St) and 11(AO) ng / g(fw). However, the decrease in inhibition of root growth lagged bchind'thc decrease in germination. It is concluded that burial is advantageous to regeneration. Inhibitors of the outer seed coat and seeds were mostly destroyed under the adverse conditions of natural dissemination (Di treatment) indicating that Di was not conducive to regeneration. MS analysis revealed that the largest peaks of No. 6, 7 and 12 zones were linoleic acid, olcic acid and 17-C, 18-C, and 20-C long chain fatty acids. Several coumarins were found in No. 6 and 7 zones, and cadincncs, farncsol and scsquitcrpcncs were found in No. 6 and 12 zones having common bioorigins with ABA.展开更多
Protein kinase inhibitor 6-DMAP was used to explore the effect of protein phosphorylation on germinal vesicle breakdown(GVBD) and chromatin/chromosome behavior of mousc oocytes at different stages .Protein phosphoryl...Protein kinase inhibitor 6-DMAP was used to explore the effect of protein phosphorylation on germinal vesicle breakdown(GVBD) and chromatin/chromosome behavior of mousc oocytes at different stages .Protein phosphorylation induced by 6-DMAP-sensitive protein kinases was not required for GVBD 2 hrs after culture. 6-DMAP stimulated transition to interphase in oocytes after GVBD and inhibited extrusion of the first polar body(PB1) and the second polar body(PB2). This drug also induced the reformation of nucleus in metaphase II(M II) oocytes spontaneously,without the release of cortical granules(CGs), a result that is different from the conclusion obtained before.展开更多
文摘Bioassay and GC-MS determination indicated that inhibitors of seeds of Fraxinus mandshurica Rupr were distributed mainly in zones of β-inhibitors (including ABA) and of high and low Rf value. The inhibitory activity of outer seed coat of the dormant seed (control treatment) was higher than that of seeds with only the inner coat attached e.g. ABA was 114 and 54ng/g fresh weight respectively. The inhibitory effect of No. 12 zone was even higher than ABA. The inhibitory activity of all the zones decreased after stratification (St) and burying (AO) treatment, especially that of the outer seed coat, whose ABA content decreased to 7(St) and 11(AO) ng / g(fw). However, the decrease in inhibition of root growth lagged bchind'thc decrease in germination. It is concluded that burial is advantageous to regeneration. Inhibitors of the outer seed coat and seeds were mostly destroyed under the adverse conditions of natural dissemination (Di treatment) indicating that Di was not conducive to regeneration. MS analysis revealed that the largest peaks of No. 6, 7 and 12 zones were linoleic acid, olcic acid and 17-C, 18-C, and 20-C long chain fatty acids. Several coumarins were found in No. 6 and 7 zones, and cadincncs, farncsol and scsquitcrpcncs were found in No. 6 and 12 zones having common bioorigins with ABA.
文摘Protein kinase inhibitor 6-DMAP was used to explore the effect of protein phosphorylation on germinal vesicle breakdown(GVBD) and chromatin/chromosome behavior of mousc oocytes at different stages .Protein phosphorylation induced by 6-DMAP-sensitive protein kinases was not required for GVBD 2 hrs after culture. 6-DMAP stimulated transition to interphase in oocytes after GVBD and inhibited extrusion of the first polar body(PB1) and the second polar body(PB2). This drug also induced the reformation of nucleus in metaphase II(M II) oocytes spontaneously,without the release of cortical granules(CGs), a result that is different from the conclusion obtained before.
