Exploration,Evaluation and Screening of Excellent Asparagus Germplasm Resources

[Objectives]This study was conducted to solve the problem of lacking excellent germplasm resources in asparagus cultivation in China.[Methods]Twenty asparagus germplasm resources were introduced from home and abroad,and evaluated and screened from biological traits,yield,quality,disease resistance and stress resistance.[Results]Six excellent asparagus cultivars were screened out.Four cultivars,Jinguan,Feicuimingzhu,Jiyulvlu3 and Potron,performed well in agronomic traits,yield,commodity quality and disease resistance,and these four varieties had strong resistance to stem wilt,so they are suitable for planting in North China.JX1502 and Jersey knight showed strong salt tolerance,and exhibited a high yield in saline-alkali soil with a soil salt content of 0.56%.indicating that they are suitable for cultivation in coastal saline-alkali areas in China.[Conclusions]This study provides a theoretical basis for the development of asparagus industry in Hebei Province.

Documentation Concordance,Sharing and Utilization of Tea Germplasm Resources in Yunnan

In this paper,the research achievements and progress of Yunnan tea germplasm resource in past sixty years are systematically reviewed from the following aspects：exploration,collecting,conservation,protection,identification,evaluation and shared utilization.Simultaneously,the current problems and the suggestions about subsequent development of tea germplasm resources in Yunnan were discussed,including superior and rare germplasm collection,tea genetic diversity research,biotechnology utilization in tea germplasm innovation,super gene exploration and function,the construction of utilization platform,biological base of species and population conservation.

ISSR Analysis of Genetic Relationship of Germplasm Resource in Prunus mume Sibe. et Zucc

Using ISSR technique to analyze the diversity and genetic relationship of germplasm resources in 39 Prunus mume Sibe. et Zucc., the result showed that 10 primers were screened with high resolution from 51 primers, 120 fragments were amplified, of which 98 were polymorphic loci, accounting for 81.67% of total. Tested materials were divided into 3 classes, as was fundamentally accorded with the traditional classification base on horticulture. There was no obvious difference in geographic relationship among the clustering results.

Research on Background and Utilization of Germplasm Resources in Maize

[Objective] The research aimed at introducing the background and utilization of germplasm resources in maize.[Method] The research progress on genetic background and utilization of maize germplasm resources was comprehensively reviewed in this paper,including maize heterotic groups and heterotic patterns,their flexible application in breeding and the utilization of various types of original materials.[Result] The results show that germplasm resource is the premise and basis for maize breeding.In-depth understanding and a reasonable selection of germplasm resources can significantly improve the breeding efficiency and speed up the breeding process.At the same time,it is considered that the selection and construction of original materials in maize breeding can not be limited to the heterotic groups and heterotic patterns,but has to be used reasonablely according to different types of germplasm resources and individual breeding objective and idea as well.[Conclusion] This study had broadened the idea and provided inspiration for the maize breeding practice in China.

Screening of Drought Stress Indicators and Analysis of Drought Resistance in Cultivated Rice Germplasm Resources in Guangxi

This study aimed to screen drought stress indicators and analyze the drought resistance in 105 cultivated rice germplasm resources in Guangxi Zhuang Autonomous Region under drought stress in the whole growth period based on D value of seven morphological traits including panicle neck thickness,effective panicle number per plant,1 000-grain weight,filled grain number per panicle,total grain number,seed setting rate and yield per plant.According to the results,under drought stress,yield per plant exhibited significant positive correlation with panicle neck thickness and exhibited extremely significant positive correlation with other five morphological traits.Based on the identification and evaluation of comprehensive drought-resistance index D value,seven highly drought-resistant cultivated rice varieties were screened,accounting for 6.67％.Upland rice germplasm resources in Guangxi Zhuang Autonomous Region possess strong drought-resistance capacity,which could be used to screen highly drought-resistant rice germplasm materials.This study provided material basis and theoretical reference for breeding drought resistant rice cultivars in Guangxi Zhuang Autonomous Region.

Study on Present Status of Plant Germplasm Resources Conservation and Countermeasures

Plant germplasm resources is the material foundation and key point for agricultural production, crop breeding and bioengineering. China is one of the centers for the origin of the cultivated plants worldwide, as well as one of the countries with most abundant plant resources and the highest biodiversity. Strengthening research in the conservation and utilization of plant germplasm resources is of significant value and importance. In this study, the present status of conservation, utilization, and existing problems of plant germplasm resources in China and around the world were reviewed; further, through analyzing measures taken by global countries for plant germplasm resources protection, countermeasures for plant germplasm resources conservation in China were also presented from three aspects, namely, collection and conservation, information network construction and national legislation and policies.

Research Progress on Germplasm Resources of Common Bean

The origin, classification, utilization and evaluation of germplasm resources, and breeding methods were introduced. The researches of biotic or/and abiotic stress, exploration of genomics and proteomics in common bean in recent years were reviewed in this paper, in order to provide theoretical reference for utilization and innovation of germplasm resources in common bean.

Fruit Anatomy of sect. Paracamellia with Reference to Their Significance of Germplasm Resources Exploitation

[Objective] To utilize the germplasm resources,new systematic evidence for the classification of sect.Paracamellia,genus Camellia is found in this article.[Method] Fruit anatomy of 10 species representing sect.Paracamellia was investigated.And the main component of sect.Paracamellia in the genus Camellia was studied by MVSP software.[Result] The exocarp was thin;mesocarp was broad,having a large number of stone cells;endocarp was lignified,and separated easily from seed.The principle components analysis suggested that the distance between species was far,showing far relation;the current study did not support the formation of Camellia grijsii（C.grijsii）and Camellia yuhsienensis（C.yuhsienensis）,Camellia shensiensis（C.shensiensis）should not be recognized as a variety of C.yuhsienensis.In addition,it also proved Ming＇s combining of Camellia confusa（C.confusa）and Camellia kissi（C.kissi）was unnatural.[Conclusion] The study provided theoretical basis for the development and application of sect.Paracamellia in the genus Camellia.

Collection and Preservation of Cashew Germplasms Resources Research Progress in China

The cashew tree is a typical tropical nut tree species in China, and the collection, identification and innovated utilization of excellent cashew germplasm resources are of great significance to increase the income of farmers and agricultural benefits in the tropical area of China. This study summarized the major advances on cashew germplasm resources in China, analyzed the major research progress and existing problems from the aspects of the collection and evaluation, resistances, genetic diversity of cashew germplasm resources, and put forward the work ideas to analyze the construction of core germplasm bank and the genetic stability of the major biological characteristics of cashew germplasm resources, which were of important significance for the improvement of the cultivar classification and resource evaluation system of cashew germplasm resources in China.

Study on Germplasm Resources of Tartary Buckwheat (Fagopyrum tataricum) in Shanxi Province

This study analyzed the tartary buckwheat recorded in the books Catalogue of Crop Germplasm Resources in Shanxi Province and Catalogue of Chinese Buckwheat Germplasm Resources by Excel 2003 and DPS 7.5 from the aspects of distribution areas,main agronomic traits and nutritional components,so as to lay a foundation for the selection of excellent buckwheat germplasm resources and improvement of buckwheat varieties.The results showed that different buckwheat varieties vary in plant height,node number of the main stem,grain weight per plant,grain weight per plant and kilo-grain weight.Compared with the national average values,buckwheat in Shanxi is higher in protein and fat,but lower in vitamin E and vitamin PP;it is rich in Ca,Mg,Cu,P,Se,Fe,Mn and Zn;and it contains 18 amino acids with the content of glutamic acid being the highest and the content of tryptophane being the lowest.After analyzing and classifying the tartary buckwheat germplasm resources recorded in Catalogue of Grop Germplasm Resources in Shanxi Province and Catalogue of Chinese Buckwheat Germplasm Resources,breeders could breed new varieties with high yield and quality and strong stress resistance by crossbreeding with these germplasm resources.

Evaluation and Screening of Aceraceae Germplasm Resources

[Objective] The aim was to screen out excellent Aceraceae germplasm resources which can be applied in gardens and urban landscape. [Method] The set of 17 biological traits of 24 Aceraceae species were observed and recorded. Taking into consideration aesthetic evaluation of different groups of people, the studied species were graded in quantitative scale. The species with higher overall score is considered better germplasm resource. [Result] The Aceraceae species with top ten highest overall scores were： Acer japonicum ＇Aconitifolium＇ （45.5）, Acer palmatum var. thunbergii （43.8）, Acer palmatum （41.8）, Acer elegantulum （39.2）, Acer elegantulum var. macrurum （39.2）, Acer rubrum （39.1）, Acer Cordatum（38.8）, Acer truncatum （38.3）, Acer olivaceum （37.7） and Acer acutum （37.3）. [Conclusion] The above ten species can be used preferentially as excellent Aceraceae germplasm resources for landscaping.

Investigation on Resources Status of Wild Platycodon grandiflourus in Changbai Mountain Area

[Objective] The aim was to investigate the status of wild Platycodon grandiflourus resources in Changbai Mountain area. [ Method] The habitats and growth environment of the wild Platycodon grandiflourus were investigated, and made a collection of the germplasm resources at 24 sites in Changbai Mountain area. [ Result ] Most of the exiting wild Platycodon grandiflourus survived because of its small roots that means no value and grew in poor or remote conditions that led to much difficulty to dig. The character of wild Platycodon grandiflourus was obviously inferior to cultivated Platycodon grandiflourus. [ Condusion] This study lays a foundation for the genetic diversity analysis and the establishment of germplasm resource pool of wild Platycodon grandflourus in Changbai Mountain Area.

Morphological Diversity Analysis of Red-seed Watermelon (Citrullus lanatus ssp. vulgaris var. megalaspermus Lin et Chao) Germplasm Resources

[Objective] This study aimed to analyze the morphological diversity of red- seed watermelon （Citrullus lanatus ssp. vulgaris var. megalaspermus Lin et Chao） germplasm resources. [Method] Multiple cluster analysis and principal components analysis on the morphological traits of 51 red-seed watermelon germplasm resources were carried out. [Result] The coefficient of variations （CVs） of 39 morphological traits in 51 red-seed watermelon idioplasm resources ranged from 5.37% to 66.95%, with an average of 22.87%. The average of Shannon diversity information indices was 1.55. Among them, the Shannon diversity information index of seed length was the highest （2.16） and that of seed shell figure pattern was the lowest （0.32）. In ad- dition, the morphological diversity information indices of quantity characters were higher than that of quality characters. The principal components analysis revealed that the variance contribution rates of the first, second and third principal compo- nents were 19.49%, 15.32% and 9.55%, respectively. Cluster analysis divided the 51 materials into three broad branches based on the morphological traits. There was only one material in the fist branch and two in the second branch, and all the three materials were wild. The other 48 materials were divided into the third branch and all of them were cultivars. [Conclusion] This study provided a theoretical basis for the protection and utilization of red-seed watermelon resources.

Track Monitoring on Viability of Rice Germplasm Resources and Regeneration under Low Temperature Storage

53 rice germplasm resources warehoused during 1981-1984 were regarded as materials to monitor the viability at warehouse time and different years after warehoused. The results showed that seed germination rates of different rice germplasm resources assumed descending trend in storage, with annual decreasing rate between 0.12%-3.05% ; the seed germination rates of most cultivars were above 75% after stored for 26 years; forecasting analysis based on the germination rate of 75% as reference showed a huge difference of safe storage life for different rice germplasm resources, ranging from 12 to 50 years, even longer time. The results suggest that track monitoring on viability and regeneration of rice cultivars is of great importance for germplasm resources conservation.

Research on Coconut Germplasm Resource in Hainan Province

The researches on coconut germplasm resource of Hainan Province and its importance were reviewed in this study.The classification and characters of coconut germplasm resource in Hainan Province,as well as its development and utilization of were summarized.In addition,some of the problems in researches on coconut germplasm resource and the potential research directions were discussed.

Characterization of 124 Flue-cured Tobacco Germplasm Resources

[Objective] This study aimed to characterize 124 flue-cured tobacco germplasm resources, and to provide reference for making the best use of the existing flue-cured tobacco germplasm resources. [Method] A total of 124 flue-cured tobacco germplasm resources were studied via field experiment and statistic analysis to investigate characteristics of their growth period, morphologic characters, main agronomic traits, and economic characters. [Result] There were significant differences among the 124 flue-cured tobacco germplasm resources in growth period, morphologic characters, main agronomic traits, and economic characters. The coefficients of variation （CV） varied in a range of 7.9%-22.5%. Among them, CV of bud stage was higher, and that of corolla length was lower. Many germplasm resources were attacked seriously by black shank disease at the end of field experiment, including those with disease index higher than 90, like Kutsaga 110, TI706, Ky 151, KM10, Longshe, Chaoxian Kangchi, Kutsaga 51E, Yongding No.1, and those with disease index among 75-90 such as Liaoyan No. 8, Lingnong No.2, Shandong Duoye, 581, Cunjingyan, KRK26, and Special 400. The tobacco root knot nematode invasion was light in some germplasm or never happened in other germplasm resources. TMV happened sporadically. CV 87, Yunyan 99, Yunyan 85, K326, Yunyan 87, Zhubuo No.1, T64 and T66 produced higher yield, production value, proportion of good leaves and sold at a higher price. Finally, we screened out the germpalsm resources with good comprehensive economic values and high disease resistance such as CV 87, Yunyan 99, Yunyan 85, K326, Yunyan 87, Zhubuo No.1, T64, T66, Honghuadajinyan, G-28, Taiyan No. 8, 77089-12, Yunyan 97, K346, V2, K149, NC567, NC297, Yunyan 201, Yunyan 203, KRK22. [Conclusion] This study provides reference for making better use of tobacco germplasm resources, and breeding new varieties.

Development and Utilization of Naturally Colored Cocoon Resources of Bombyx mori

This paper summarized the diversity and prominent characteristics of naturally colored cocoon resources of Bombyx mori, and introduced the production methods of colored cocoons, the development and utilization of naturally colored cocoon resources at home and abroad as well as the variety breeding instances, based on which suggestions were proposed for the development of colored cocoon industry.

Genetic diversity and population genetic structure of Paeonia suffruticosa by chloroplast DNA simple sequence repeats(cpSSRs)

Paeonia suffruticosa Andr.is an endemic shrub flower in China with 2n=10.This study used 228 cultivars from four populations,i.e.,Jiangnan,Japan,Northwest,and Zhongyuan,as materials to explore the genetic diversity levels among different populations of tree peony varieties.The results showed that 34 bands were amplified using five pairs of cp SSR primers,with an average of 6.8 bands per primer pair.The average number of different alleles(N_(a)),effective alleles(N_(e)),Shannon's information index(I),diversity(H),and polymorphic information content(PIC)were 3.600,2.053,0.708,0.433,and 0.388,respectively.The PIC value was between 0.250 and 0.500,indicating a moderate level of polymorphism for the five cp SSR primer pairs.The genetic diversity levels of peony cultivars varied among different populations,with the Northwest population showing relatively lower levels(I=0.590,H=0.289,and PIC=0.263).A total of 52 haplotypes were identified in the four examined populations,and the number of haplotypes per population ranged from 11 to 22.Forty-four private haplotypes were detected across populations,and the Northwest population exhibiting the highest count of private haplotypes with 17.The mean number of effective number of haplotypes(N_(eh)),haplotypic richness(R_h),and diversity(H)were 8.351,6.824,and 0.893,respectively.Analysis of molecular variance indicated that genetic variation within tree peony germplasm was greater than that between germplasm resources,and the main variation was found within individuals of peony germplasm.Cluster analysis,principal coordinate analysis,and genetic structure analysis classified tree peonies from different origins into two groups,indicating a certain degree of genetic differentiation among these four tree peony cultivation groups.This study provides a theoretical basis for the exploration,utilization,and conservation of peony germplasm resources,as well as for research on the breeding of excellent varieties.

Germplasm resources of three wood plant species enriched with nervonic acid

Nervonic acid(NA)is a very-long-chain monounsaturated fatty acid with pharmaceutical and nutraceutical functions that plays an important role in treating several neurological disorders.One major source of NA is plant seed oil.Here we report fatty acid profiles of seeds and germplasm diversity of six plant species,including three woody plants with high amounts of NA-enriched seed oil,Malania oleifera,Macaranga adenantha,and M.indica.M.oleifera had the largest seed(average 7.40 g single seed),highest oil content(58.71%),and highest NA level(42.22%).The germplasm diversity of M.oleifera is associated with its habitat but not elevation.Seeds of M.adenantha contained higher NA levels(28.41%)than M.indica(21.77%),but M.indica contained a significantly higher oil content(29.22%)and seed yield.M.adenantha germplasm varied among populations,with one population having seeds with high oil content(22.63%)and NA level(37.78%).Although M.indica grow naturally at a range of elevations,no significant differences were detected between M.indica populations.These results suggest that M.indica and M.oleifera have greater potential as a source of NA,which will contribute to constructing a germplasm resource nursery and establishing a selection and breeding program to improve the development of NA-enriched plants.

Identification of Cold Tolerance of Rice Germplasm Resource at Germinating Stage in Cold Region

Total 75 rice varieties （lines） in Heilongjiang Province （or cold region） as germplasm resources were identified for cold tolerance at germinating stage by controlling temperature in artificial incubator. The results showed that the shooting seed rate at the germinating stage could be used as the evaluation index of cold tolerance. The cold tolerance was recorded on 1-9 scale and could be identified by the criteria of five indexes such as highly tolerant （HT）, tolerant （T）, moderately tolerant （MT）, susceptible （S）, highly susceptible （HS）.