Sweet Cherry (<i>Prunus avium</i>L.) Fruit Drop Reduction by Plant Growth Regulators (Naphthalene Acetic Acid NAA and Gibberellic Acid GA₃)

This study was conducted during 2019/2020 on sweet cherry trees (Prunus Avium L.) (Bing and Hardy Giant) cultivar planted at Sergaya-Al_Zabadani area of Rural Damascus, to reduce fruit drop of sweet cherry. The experiment included 4 foliar applications: T1: control, T2: GA3 (100 ppm), T3: NAA (20 ppm), T4: (100 ppm GA3 + 20 ppm NAA). Fruit set and fruit drop percentage, fruiting factor, and yield were recorded. The results showed that treatment with (100 ppm GA3 + 20 ppm NAA) recorded higher fruit set percentage (73.81% and 75.62%), and fruiting factor (48.38% and 50.04%) respectively; In addition to fruit yield (40.19 and 41.21 kg/tree) for both cultivars, compared to the control (9.13, 6.60 kg/tree). Therefore, it can be concluded that GA3 + NAA treatment reduced Sweet cherry fruit drop better than other treatments, where fruit drop percentage didn’t exceed (63.11% and 62.01%) in both cultivars (Bing and Hardy Giant) respectively, compared to the control (80.92% and 80.64%).

外源GA_(3)对水分胁迫下川黄柏抗性的影响

【目的】为实际栽培中利用外源激素提高川黄柏苗期成活率提供参考。【方法】以1年生川黄柏幼苗为试材,在自然渍水及干旱胁迫下,采用不同浓度的外源GA_(3)进行不同周期的喷施处理,测定分析各处理下川黄柏幼苗渗透调节物质含量、抗氧化酶活性、抗氧化剂含量和膜脂过氧化产物含量等生理指标的变化特征。【结果】在渍水胁迫下,外源喷施GA_(3)溶液提高了川黄柏可溶性糖、可溶性蛋白和还原型谷胱甘肽(GSH)的含量,增加了超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性,且与对照组相比差异显著(P<0.05),丙二醛(MDA)含量总体呈增加趋势,但与对照组相比差异不显著(P>0.05)。复水后0~2 d,干旱胁迫下的对照组及GA_(3)处理组(200 mg/L,喷施间隔30 d)植株各生理指标均逐步恢复正常,受间断降水影响;复水后3~5 d,对照组及GA_(3)处理组植株各生理指标出现反复变化。复水后0~5 d时,与对照组相比,GA_(3)处理提高了植株的可溶性糖含量、可溶性蛋白含量、SOD活性、POD活性、CAT活性及GSH含量,并且加快了这些指标的恢复速度,降低了MDA含量。水分胁迫条件下,大多数指标间呈现不显著的相关关系,呈显著或极显著相关的指标间均为正相关关系。干旱和渍水胁迫条件下,激素处理组总体上表现为促进植株生理指标向适应环境的方向改变,短周期喷施高浓度GA_(3)的促进效果较为明显,且喷施周期与喷施浓度有交互效应。【结论】在川黄柏早期栽培中,适当喷施GA_(3)可以提高植株对渍水胁迫和干旱胁迫的适应能力。

IAA和GA_3对棉花短纤维突变体纤维长度的离体诱导作用

以棉花等基因系超短纤维突变体 (Ligoncotton ,Li1 )及其野生型 (li1 )为材料 ,用胚珠离体培养方法 ,研究IAA(生长素 )和GA3(赤霉酸 )与纤维细胞伸长的关系。研究表明 :(1)在含单激素IAA或GA3培养基内 ,离体诱导突变体胚珠产生的纤维长度分别约为 1 86mm和 2 1mm ,比在对照 (不含激素 )培养基内的纤维长度分别增长 86 %和 110 % ,说明外源激素IAA或GA3对突变体纤维伸长能力的缺损有补偿作用。 (2 )在同时含有IAA和GA3两种激素的培养基中 ,突变体和野生型胚珠产生的纤维长度分别为 3 4 1mm和 7 2 3mm ,是它们在田间自然条件下纤维长度的 76 %和 2 4 3% ,均显著高于只含一种激素培养基内的纤维长度 ,说明IAA与GA3间在诱导纤维伸长上有协同作用 ,而且 ,这种作用对突变体比对野生型大 3 1倍 ,即在一定程度上能解除突变体纤维伸长受到的阻碍。 (3)突变体胚珠在含GA3和IAA培养基内经培养2 1d后 ,胚珠IAA和GA3平均含量 ,虽稍高于对照培养基内野生型胚珠的含量 ,但明显小于GA3和IAA培养基内野生型胚珠的平均含量 ,而且其纤维长度也显著小于野生型的纤维长度。说明在离体培养条件下 ,突变体的纤维伸长比野生型需更高的胚珠GA3和IAA水平 ,但由于突变体在遗传上的缺损 。

采后GA_3诱导菠萝多酚氧化酶活性升高的机理

在多酚氧化酶（ＰＰＯ）提取过程中，直接加入ＧＡ３溶液，不能使ＰＰＯ活性增加。采后ＧＡ３诱导菠萝（Ａｎａｎａｓｃｏｍｏｓｕｓ（Ｌ．）Ｍｅｒ．）ＰＰＯ活性升高的同时，显著提高了酸性磷酸酯酶的活性，这是果实组织的Ｐｉ浓度明显升高的主要原因。随着果实的后熟，磷脂酶Ｄ活性不断下降，ＧＡ３处理显著延缓了该酶的下降速度。虽然采后ＡＢＡ处理使果实ＰＰＯ活性有小幅度增加，但ＡＢＡ处理能使ＧＡ３对ＰＰＯ活性的诱导明显加强。

外源IAA和GA_3对杨树应拉木形成的影响

【目的】探索外源激素吲哚乙酸(IAA)和赤霉素(GA_3)对杨树应拉木形成的影响,揭示杨树应拉木的形成机理。【方法】以人工倾斜45°的当年生中林46杨枝条为试材,连续施加不同含量的IAA(0.01,0.10和1.00mg/g)和GA_3(0.10,1.00和10.00mg/g)对枝条生长进行调控,分别观测不同处理下枝条的生长量、角度、偏心率、纤维形态、组织比量和胶质纤维比率,分析这2种外源激素对杨树应拉木形成的影响。【结果】施加1.00 mg/g外源IAA抑制了纤维细胞的生长及胶质纤维和导管的形成,不仅枝条角度变大,而且枝条生长受到限制;施加1.00和10.00mg/g外源GA_3可促进纤维细胞、胶质纤维的形成和枝条的生长,使枝条偏心率显著增大,且高含量GA_3的作用效果更明显;0.01mg/g IAA和0.10mg/g GA_3对枝条的作用效果基本一致,均促进了枝条横向疏导组织的形成,使其偏心率明显减小,但对倾斜枝条应拉木形成的作用并不明显。【结论】IAA和GA_3均能够影响杨树应拉木的形成,高含量的IAA明显抑制应拉木的形成,而高含量的GA_3可明显促进应拉木的形成。

外源GA_(3)对紫斑牡丹幼苗叶片解剖结构与光合特性及内源激素含量的影响

以1年生紫斑牡丹幼苗为试验材料,采用不同浓度(0、100、300、500 mg/L)赤霉素(GA_(3))喷施叶片处理,通过透射电镜、扫描电镜、光学显微镜观察幼苗叶片解剖结构,光合仪测定幼苗光合参数并以酶联免疫吸附法测叶片内源激素含量,探究外源GA_(3)对紫斑牡丹幼苗叶片解剖结构、光合特性和内源激素水平的影响。结果表明:(1)低浓度GA_(3)处理的紫斑牡丹叶肉细胞增大,栅栏组织外层细胞中叶绿体数量增加,高浓度GA_(3)处理则与之相反;GA_(3)处理叶片的栅栏组织/海绵组织比值(P/S)、组织结构紧密度(CTR)均下降,而其组织结构疏松度(SR)增加;GA_(3)处理的幼苗叶片的叶肉细胞内各叶绿体大小显著大于对照,随着GA_(3)处理浓度增加,紫斑牡丹叶肉细胞内叶绿体的体积趋于增大,类囊体垛叠凝聚逐渐松散,叶绿体上淀粉颗粒在300 mg/L GA_(3)处理中较明显;叶片气孔长度、宽度、气孔器大小、气孔开度和气孔密度随着GA_(3)浓度升高先升高后下降,同时叶片上表皮角质层厚度随GA_(3)浓度的升高而增加。(2)紫斑牡丹叶片净光合速率(Pn)、气孔导度(Cond)、蒸腾速率(Tr)、水分利用率(WUE)在100和300 mg/L GA_(3)处理下大都显著高于对照,且300 mg/L GA_(3)处理显著高于其余处理,而其在500 mg/L GA_(3)处理下显著低于对照。(3)紫斑牡丹叶片脱落酸(ABA)和吲哚乙酸(IAA)含量均在500 mg/L GA_(3)下显著高于对照,而在其余浓度处理下不同程度低于对照,叶片内源玉米素核苷(ZR)和GA_(3)含量均在300 mg/L GA_(3)处理下显著高于其余处理和对照,而其余处理相比对照均无显著变化;叶片的ZR/ABA、ZR/IAA、ZR/GA_(3)和(IAA+GA_(3)+ZR)/ABA比值都在300 mg/L GA_(3)处理下显著高于其他处理,叶片的IAA/ABA和ABA/GA_(3)比值均在500 mg/L GA_(3)处理下显著高于其他处理。研究发现,适宜浓度外源GA_(3)处理,能显著提高紫斑牡丹幼苗叶片光合速率、水分利用效率及蒸腾速率,调节植物体内源激素的含量及平衡,从而使叶片能合成较多有机物,促进幼苗生长。

Effect of exogenous GA3 on flowering quality, endogenous hormones, and hormone-and flowering-associated gene expression in forcing-cultured tree peony（Paeonia suffruticosa）

Gibberellins(GAs)promote flowering in the forcing-cultured tree peony(Paeonia suffruticosa),however,the mechanism of regulating flowering is not fully understood.In this study,exogenous GA3 was applied to five-year-old Luoyang Hong plants to explore responses in terms of endogenous hormones,flowering quality,and the hormone-and flowering-associated gene expression.Exogenous GA3 application significantly promoted flower bud development and new branch growth,as well as improved flowering quality.Exogenous GA3 application also stimulated the synthesis of endogenous GA3 and indole-3-acetic acid(IAA)but reduced abscisic acid(ABA)levels.To further elucidate the regulatory mechanism,eight genes for GA biosynthesis and signaling,including PsCPS,PsKS,PsGA3ox,PsGA2ox,PsGID1b,PsGID1c,PsDELLA,and PsGID2 were cloned for the first time,and sequence analysis was also performed.The results suggested that all the cloned genes have conserved structure as each homologous gene reported in the other species.Phylogenetic trees constructed by the each cloned gene showed that the phylogenetic evolutionary relationship of P.suffruticosa was closely related to Vitis vinifera.The expression patterns of the above genes,and genes for ABA and IAA biosynthetic and signaling,and the flowering time were also investigated.Most of the above genes showed higher expression in the control buds than those in the GA3 treated buds at six developmental stages,whereas the expression levels of PsSOC1 and PsSPL9 were up-regulated by GA3 treatment.The results also showed that the GA-biosynthetic and signaling pathways are conserved in tree peony,and the PsCPS,PsGA3ox,PsGA2ox,PsGID1,PsDELLA,and PsGID2 genes are necessary for feedback regulation of GAs.Furthermore,hormone changes promoted PsSOC1 and PsSPL9 expression,and repressed PsSVP expression,which contributed to the improvement flowering quality in tree peony of forcing culture.

Roles of abscisic acid and gibberellins in maintaining primary and secondary dormancy of Korean pine seeds

Primary dormancy of seeds of Korean pine(Pinus koraiensis Sieb.et Zucc.)after dispersal in the autumn and the induction of secondary dormancy the fi rst summer following seed dispersal limit the regeneration of mixed broadleaved Korean pine forests in Northeast China.This study was to determine how changes in the levels of abscisic acid(ABA)and gibberellic acid(GA)maintain primary and secondary dormancy of Korean pine seeds under germination conditions.We transferred seeds with one of fi ve primary dormancy states or three secondary dormancy states to germination conditions and measured changes in the levels of ABA,GA 1+3(GA 1 and GA 3)and GA 4+7(GA 4 and GA 7)in the seed coat,megagametophyte and embryo during incubation.Seed coat ABA levels in primary dormant seeds(PDS)and ABA levels in various parts of secondary dormant seeds(SDS)gradually declined during incubation but were still higher than in seeds for which dormancy was progressively released.GA 4+7 and GA 1+3 levels in embryos greatly decreased 35%and 24%,respectively,during incubation of SDS,and thus,the ratio of ABA to GA 4+7 in embryos and megagametophytes signifi cantly increased.The ratio of ABA to GA 1+3 in various parts of SDS increased slightly during incubation.In contrast,in seeds for which secondary dormancy was already released,GA 4+7 and GA 1+3 levels in the embryo,GA 4+7/ABA ratio in the embryo and seed coat,and the GA 1+3/ABA in the embryo and megagametophyte signifi cantly increased during incubation.There was no trend in the changes in the levels of ABA,GA 4+7 or GA 1+3 in embryos and megagametophytes of PDS or the levels of GA 4+7 or GA 1+3 in megagametophytes of SDS during incubation.The results suggest that high ABA levels in the seed coat maintain primary dormancy of Korean pine seeds.Maintenance of secondary dormancy involves a reduction of GA 4+7,GA 1+3,GA 4+7/ABA,and GA 1+3/ABA and the retention of high ABA levels.

气相色谱法测定7-氨基-3-(丙烯-1-基)-3-头孢烯-4-羧酸中溶剂残留量

建立气相色谱法同时测定7-氨基-3-(丙烯-1-基)-3-头孢烯-4-羧酸中丙酮、四氢呋喃、甲醇和二氯甲烷溶剂残留量的方法。7-氨基-3-(丙烯-1-基)-3-头孢烯-4-羧酸样品使用0.1%氢氧化钠溶液进行超声溶解,使用HP-FFAP毛细管柱进行化合物的分离,氢离子火焰检测器进行测定,外标法定量。丙酮、甲醇、二氯甲烷和四氢呋喃均能获得基线分离,甲醇、丙酮、四氢呋喃和二氯甲烷的质量浓度分别在5.1~657.6 mg/L,2.1~677.1 mg/L,2.3~681.9 mg/L和2.4~728.0 mg/L范围内与其色谱峰面积具有良好线性关系,线性相关系数为0.9994~0.9999,甲醇、丙酮、四氢呋喃和二氯甲烷定量限分别为107.1、52.9、57.5、60.0 mg/kg。丙酮、甲醇、二氯甲烷和四氢呋喃测定结果的相对标准偏差均不大于2.56%(n=6),平均加标回收率为92.53%~109.96%。该方法适用于7-氨基-3-(丙烯-1-基)-3-头孢烯-4-羧酸中溶剂残留量的定性、定量分析。

Effect of Spraying with GA₃on the Quality and Oil Content of Syrian Okra Seeds <i>Abelmoschus esculentus</i>(L.)

This study aimed to estimate the effect of spraying of okra plants, Abelmoschus esculentus (L.) with GA3 on the quality and oil content of the seeds. For this, the local okra variety was selected and used, which was growing widespread in the coastal region of Syria. This study was carried out in Al-Haffa area, Lattakia governorate, Syria (with 105 MSL elevation) for two seasons 2019-2020. The results showed that the effect of spraying with GA3 was more evident in the quality of the seeds as well as oil content at 75 ppm concentration with 68.7 g weight of 1000 seeds and 5.31% seed oil percentage. This study also showed that oil of local okra variety seed was rich in the essential fatty acids namely, linoleic, palmitic, oleic, stearic and linolenic, treatment with 75 ppm concentration of GA3 was more effective in the seed content of essential fatty acids with 42.30%, 32.55%, 9.20%, 6.73% and 5.72%, respectively. Finally, our results were indicated that treatment of okra plants at 75 ppm of GA3 enhanced the quality and oil content of seeds compared to the other concentrations.

GA和SA互作对黄花蒿生长及青蒿素合成的影响

以青蒿素为基础的联合疗法是当今治疗疟疾的最有效、最重要的手段.近年来随着研究的深入,青蒿素越来越多的药理作用,如抗肿瘤、抗糖尿病等被发现和应用研究,因此青蒿素市场需求量极大.本课题旨在研究水杨酸(salicylic acid,SA)、赤霉素(gibberellic acid,GA)、水杨酸与赤霉素互作(SA+GA)对黄花蒿(Artemisia annua L.)植株的生长和青蒿素质量比的影响.研究表明,SA诱导会显著降低黄花蒿植株的农艺性状、生理生化指标以及青蒿素合成关键酶基因的表达水平,并降低青蒿素质量比.GA处理会显著增高黄花蒿植株的大部分农艺性状、生理生化指标,以及青蒿素合成相关基因的表达量,促进青蒿素生物合成;而SA+GA处理会产生拮抗作用,影响黄花蒿的农艺性状、生理生化指标,以及青蒿素合成相关基因的表达量,从而影响青蒿素的生物合成.

Separation of gibberellic acid (GA3) by macroporous adsorption resins

The adsorption effects of several macroporousadsorption resins for gibberellic acid (GA3) were investigated.The dynamic adsorption capacity is 58.38 mg/g drybeads for resin R4 and 96.46 mg/g dry beads for resin R5which is consistent with the surface area. Aqueous methanol(50%, V/V) is a good eluent and the yield of GA3 isabove 95%. The concentration of GA3 could increase fivefoldafter an adsorption-elution cycle and this is importantwhen considering further crystallization of GA3 in anindustrial process.

Effects of Gibberellic Acid (GA_(3)) and Salicylic Acid (SA) postharvest treatments on the quality of fresh Barhi dates at different ripening levels in the Khalal maturity stage during controlled atmosphere storage

Barhi dates at Khalal maturity stage are well-known with their pleasant taste,crispy texture,and bright yellow color.It is necessary to extend the duration of Barhi Khalal stage which is too short for effective marketing.This study aimed to inspect the effects of Gibberellic Acid(GA_(3))and Salicylic Acid(SA)postharvest treatments on retaining the high quality of Khalal Barhi fruits during controlled atmosphere storage.Fresh samples of Barhi fruits at Khalal stage harvested at three different ripening levels were dipped after harvesting in GA3(150 ppm)or SA(2.0 mmol/L)and subsequently stored in controlled atmosphere(0°С,5%O_(2),5%CO_(2),80%±5%RH).The results revealed that the GA_(3) and SA treatments reduced the percentage of weight loss and decay in the fruits,while the total soluble solids increased.Moreover,GA_(3) and SA treatments were significantly efficient in limiting the changes in fruit color and texture of Barhi dates compared to the control.Sensorial results support the experimental data and disclosed that the GA_(3)(150 ppm)treatment in the controlled atmosphere(CA)storage was better in conserving the quality of Barhi at the Khalal maturity stage and delaying ripening process.

3-羟基丁酸的气相色谱分析

采用ＢＦ３－ＣＨ３ＯＨ酯化衍生法，以正辛酸作内标，建立了３－羟基丁酸、巴豆酸、３－羟基丁醛、巴豆醛等混合体系中３－羟基丁酸的气相色谱分析方法。利用标准样对照并结合质谱分析确定了化学合成３－羟基丁酸反应混合物的组成。该法线性范围为０．４０～１６０．ｇ／Ｌ，相关系数为ｒ＝０．９９９５。方法快速、简单、重现性好，并用于化学合成３－羟基丁酸反应混合物的分析，结果令人满意。

毛细管气相色谱法测定海狗油胶丸中ω-3多不饱和脂肪酸的含量

目的:用毛细管气相色谱法测定海狗油中二十碳五烯酸(EPA)、二十二碳五烯酸(DPA)和二十二碳六烯酸(DHA)的含量。方法:采用HP-INNOWAX毛细管柱(30 m×0.320 mm,0.25 μm),柱温150℃,保持5 min后,以10℃·min-1升至200℃后,继续以5℃·min-1升至240℃,保持7 min,载气为氮气(99.999%)。进样口温度为240℃,检测器温度为270℃,FID检测器。结果:EPA甲酯平均回收率为99.5%,RSD为1.2%;DPA甲酯平均回收率为98.8%,RSD为1.1%;DHA甲酯平均回收率为98.5%,RSD为0.9%。结论:此法操作简单、准确,重复性、稳定性好,是一种可行的ω-3多不饱和脂肪酸含量测定方法。

酱油中3-氯-1,2-丙二醇的气相色谱-质谱分析

建立测定酱油中的痕量 3 氯 1 ,2 丙二醇 (3 MCPD)的方法。用PR小柱预分离试样 ,用苯基硼酸作为衍生化试剂 ,气相色谱 质谱联用测定。其线性范围为 0 .0 0 1～ 1 0mg kg;相对标准偏差为 1 .5 % ;检测下限为0 .0 0 1mg kg。该方法已成功应用于酱油中痕量 3

梨幼果中脱落酸和吲哚-3-乙酸的简易气相色谱分析

采用溴甲基五氟苯(PFB-Br)对其衍生、酯化成各具有5个氟的两种衍生物,直接进样测定梨幼果中的脱落酸和吲哚-3-乙酸的含量,能减少前处理的复杂步骤,脱落酸和吲哚-3-乙酸回收率分别为95.8%、84.5%,具有较高的可靠性。

气相色谱法测定用重氮甲烷预处理的2-甲基-3-硝基苯甲酸

建立了一种采用气相色谱测定 2 甲基 3 硝基苯甲酸含量的分析方法。用重氮甲烷对 2 甲基 3 硝基苯甲酸进行酯化预处理 ,以CP Sil 43CB石英毛细管柱 (2 5m× 0 32mmi d × 0 2 μm)分离 ,氢火焰离子化检测器检测 ,归一化法定量。平均回收率为 99 81 % ,相对标准偏差 (RSD)为 0 0 8% ,最低检出限为 3× 1 0 - 1 1 g。方法简便实用 ,稳定可靠 ,实现了用气相色谱法对这种高沸点、高纯度物质的定量分析 ,可用于该类物质的纯度检验 ,也可用于新产品开发及生产。

反相气相色谱法表征1-烯丙基-3-甲基氯代咪唑离子液体的表面性质

采用反相气相色谱(IGC)技术研究了不同温度下1-烯丙基-3-甲基氯代咪唑([AMIM]Cl)的表面性质。以正己烷、正庚烷、正辛烷和正壬烷作为非极性探针分子测定[AMIM]Cl在343.15、353.15、363.15和373.15 K温度下的表面色散自由能;以二氯甲烷、三氯甲烷、丙酮、乙酸乙酯、四氢呋喃作为极性探针分子测定离子液体Lewis酸碱性质,并测定了吸附自由能和吸附自由焓变等热动力学参数。实验结果表明,[AMIM]Cl的酸解离平衡常数Ka为0.34,碱解离平衡常数Kb为1.68,其表面呈Lewis两性偏碱性特点。在343.15、353.15、363.15和373.15 K温度下,[AMIM]Cl的表面色散自由能分别为52.26、50.82、46.08和42.05 mJ/m2。这一结果对研究离子液体的表面性质及应用有指导作用。

Effects of Exogenous Plant Hormones on Growth Status and Secondary Metabolism of Houttuynia cordata Thunb.

[Objectives]To improve the yield and secondary metabolite content of medicinal plants and to further develop and utilize the medicinal and other functions of medicinal plants.[Methods]We used the sterile tissue culture method with Houttuynia cordata Thunb.as the research object.Different concentrations of 1-naphthalene acetic acid(NAA),auxin(indole-3-acetic acid,IAA)and gibberellin acid(GA_(3))were added to the group culture medium of H.cordata to investigate the effects of exogenous plant hormones on plant height,root length,fresh weight,morphological characteristics,four phenolics and 20 volatile compounds.[Results]The results showed that the exogenous plant hormone of 3 mg/L GA_(3)significantly increased plant height by 79.9%over the control;the exogenous plant hormone of 3 mg/L IAA significantly increased root length by 52.6%over the control;and the exogenous plant hormone of 1 mg/L GA_(3)significantly increased fresh weight of single plant by 458.2%over the control.In the treatment group of 1 mg/L NAA,chlorogenic acid content was significantly increased by 52.6%compared with the control;in the treatment group of 1 mg/L IAA,chlorogenic acid,rutin,isodendrin and quercetin content were significantly increased by 109.1%,100.6%,173.8%,and 198.7%compared with the control,respectively;in the treatment of 3 mg/L GA_(3),chlorogenic acid,rutin,isoquercitin,and quercitin content were significantly increased by 65.3%,104.9%,139.0%and 191.2%over the control.In addition,the content of volatile compounds was significantly higher in all H.cordata treated with exogenous plant hormones of 2 mg/L NAA,1 mg/L IAA,and 3 mg/L GA_(3);however,the content of volatile compounds was lower in all of the treatments with 2 mg/L GA_(3).[Conclusions]Different exogenous plant hormones have certain effects on the growth morphology and secondary metabolic content of H.cordata,which provides theoretical basis and technical support for the development and utilization of medicinal plants.