Periodontitis, is an infectious ailment of multifactorial origin, that brings about destruction of bone and surrounding tissues. There are various oral pathogens that may be responsible for the destruction. The host e...Periodontitis, is an infectious ailment of multifactorial origin, that brings about destruction of bone and surrounding tissues. There are various oral pathogens that may be responsible for the destruction. The host encounters these microbial invasions and their products by the production and release of inflammatory mediators from the cells within the body. Glutathione-S-transferase (GST) are a group of enzymes that utilize glutathione in conditions resulting in oxidative stress. These enzymes play a key role in the detoxifycation of such substance. It aids in preventing damage to important cellular components caused by release of free reactive oxygen species. Ceruloplasmin is a ferroxidase enzyme. It plays a role as an anti-inflammatory agent, by its ability to scavenge free radicals within the body. The present study was targeted at evaluating the levels of Glutathione-S-Transferase (GST) and Ceruloplasmin as diagnostic markers for patients with chronic periodontitis in gingival crevicular fluid (GCF) and the gingival tissues. Thirty patients were divided into two groups. Experimental group comprising of 15 subjects with chronic perio- dontitis and the control group was composed of 15 healthy individuals. Highly significant changes in GST between the diseased and normal patients (P = 0.001) were detected. There was a decrease in GST level in both gingival tissue & GCF in diseased patients when compared to the control patients. The ceruloplasmin levels in GCF and gingival tissues showed no difference between the control and diseased group. Hence,these results indicate a relationship suggesting that GST produced during chronic inflammation could be used as biomarker that indicate periodontal disease .展开更多
目的:观察3种口腔护理方法对慢性牙周炎患者牙周临床指标的影响。方法:选择牙周基础治疗1个月的中、重度慢性牙周炎患者60例,随机分为3组(n=20),A组用奥硝唑+复方氯己定漱口;B组用30 m L/L过氧化氢棉签擦拭;C组用生理盐水漱口。检查并...目的:观察3种口腔护理方法对慢性牙周炎患者牙周临床指标的影响。方法:选择牙周基础治疗1个月的中、重度慢性牙周炎患者60例,随机分为3组(n=20),A组用奥硝唑+复方氯己定漱口;B组用30 m L/L过氧化氢棉签擦拭;C组用生理盐水漱口。检查并记录基线和1、3个月的探诊深度(PD)、菌斑指数(PLI)、附着丧失(AL)以及龈沟液(GCF)量,并采用酶联免疫吸附法检测GCF中基质金属蛋白酶(MMP-3)的水平。结果:与基线相比,在口腔护理后1、3个月,A、B组PD、PLI、GCF量、MMP-3水平均显著降低(P<0.05),AL仅A组在3个月时显著降低(P<0.05);而C组的各项牙周临床指标均无明显变化(P>0.05)。结论:奥硝唑+复方氯己定漱口和30 m L/L过氧化氢擦拭的口腔护理方式对慢性牙周炎患者的牙周临床指标有明显抑制作用,且奥硝唑+复方氯己定漱口的疗效显著。展开更多
目的:探讨慢性牙周炎患者龈沟液中核因子-κB受体活化子配体(receptor activator for NF-κBligand,RANKL)和骨保护素(osteoprotegerin,OPG)的变化及意义。方法:采用滤纸条法收集23例正常对照者和34例慢性牙周炎患者龈沟液(gingival cre...目的:探讨慢性牙周炎患者龈沟液中核因子-κB受体活化子配体(receptor activator for NF-κBligand,RANKL)和骨保护素(osteoprotegerin,OPG)的变化及意义。方法:采用滤纸条法收集23例正常对照者和34例慢性牙周炎患者龈沟液(gingival crevicular fluid,GCF)标本,ELISA法测定上清液RANKL和OPG含量,利用Optimas5.0图像分析软件对检测牙的根尖片进行灰度分析。结果:对照组和慢性牙周炎组临床指标(PD、AL、PLI和SBI)之间存在显著性统计学差异(P<0.01)。2组GCF中RANKL、OPG和RANKL/OPG比值之间存在显著性统计学差异(P<0.01)。慢性牙周炎组GCF中OPG浓度与PD和AL之间存在负相关关系(分别为P<0.01和P<0.05),RANKL浓度及RANKL/OPG比值与根尖片灰度值之间存在负相关关系(P<0.05),GCF中RANKL和OPG浓度及RANKL/OPG比值与PLI和SBI之间无相关关系(P>0.05)。结论:RANKL和OPG在慢性牙周炎患者的牙槽骨组织破坏过程中发挥作用。展开更多
文摘Periodontitis, is an infectious ailment of multifactorial origin, that brings about destruction of bone and surrounding tissues. There are various oral pathogens that may be responsible for the destruction. The host encounters these microbial invasions and their products by the production and release of inflammatory mediators from the cells within the body. Glutathione-S-transferase (GST) are a group of enzymes that utilize glutathione in conditions resulting in oxidative stress. These enzymes play a key role in the detoxifycation of such substance. It aids in preventing damage to important cellular components caused by release of free reactive oxygen species. Ceruloplasmin is a ferroxidase enzyme. It plays a role as an anti-inflammatory agent, by its ability to scavenge free radicals within the body. The present study was targeted at evaluating the levels of Glutathione-S-Transferase (GST) and Ceruloplasmin as diagnostic markers for patients with chronic periodontitis in gingival crevicular fluid (GCF) and the gingival tissues. Thirty patients were divided into two groups. Experimental group comprising of 15 subjects with chronic perio- dontitis and the control group was composed of 15 healthy individuals. Highly significant changes in GST between the diseased and normal patients (P = 0.001) were detected. There was a decrease in GST level in both gingival tissue & GCF in diseased patients when compared to the control patients. The ceruloplasmin levels in GCF and gingival tissues showed no difference between the control and diseased group. Hence,these results indicate a relationship suggesting that GST produced during chronic inflammation could be used as biomarker that indicate periodontal disease .
文摘目的:观察3种口腔护理方法对慢性牙周炎患者牙周临床指标的影响。方法:选择牙周基础治疗1个月的中、重度慢性牙周炎患者60例,随机分为3组(n=20),A组用奥硝唑+复方氯己定漱口;B组用30 m L/L过氧化氢棉签擦拭;C组用生理盐水漱口。检查并记录基线和1、3个月的探诊深度(PD)、菌斑指数(PLI)、附着丧失(AL)以及龈沟液(GCF)量,并采用酶联免疫吸附法检测GCF中基质金属蛋白酶(MMP-3)的水平。结果:与基线相比,在口腔护理后1、3个月,A、B组PD、PLI、GCF量、MMP-3水平均显著降低(P<0.05),AL仅A组在3个月时显著降低(P<0.05);而C组的各项牙周临床指标均无明显变化(P>0.05)。结论:奥硝唑+复方氯己定漱口和30 m L/L过氧化氢擦拭的口腔护理方式对慢性牙周炎患者的牙周临床指标有明显抑制作用,且奥硝唑+复方氯己定漱口的疗效显著。
