Mesenchymal stem cells （MSCs） derived from bone marrow are a well-characterized population of adult stem cells that can be maintained and propagated in culture for a long time with the capacity to form a variety of ...Mesenchymal stem cells （MSCs） derived from bone marrow are a well-characterized population of adult stem cells that can be maintained and propagated in culture for a long time with the capacity to form a variety of cell types. This study investigated the characteristics of dairy goat bone marrow MSCs （gMSCs） and their differentiation potential toward germ cells in vitro, and to test their potential in vivo, these ceils were transplanted into seminiferous tubes of endogenous germ cells-depleted mouse models. The results showed that characteristic gMSC lines were established and a small population of gMSCs transdifferentiated into male germ cell-like cells which expressed Stra8 after induction with retinoic acid （RA）, as analysed by reverse transcription-polymerase chain reaction （RT-PCR） and immunofluorescence. Further, we transplanted the gMSCs into endogenous germ cells-depleted mouse models. A variety of analysis demonstrated that gMSCs might differentiate into male germ cells and helped spermatogenesis in endogenous germ cells depleted mouse models at 30 d after transplantation. The gMSCs could be used as a potential source of cells for reproductive studies and a neoadjuvant therapy for the spermatogenesis anomaly. Moreover, these cells may offer a new strategy for male infertility and an alternative approach for production of transgenic animals.展开更多
The gingiva, the masticatory portion of the oral mucosa, is excised and discarded frequently during routine dental treatments and following tooth extraction, dental crown lengthening, gingivectomy and periodontal surg...The gingiva, the masticatory portion of the oral mucosa, is excised and discarded frequently during routine dental treatments and following tooth extraction, dental crown lengthening, gingivectomy and periodontal surgeries. Subsequent to excision, healing eventually happens in a short time period after gingival surgery. Clinically, the gingival tissue can be collected very easily and, in the laboratory, it is also very easy to isolate gingival-derived mesenchymal stem cells (GMSCs) from this discarded gingival tissue. GMSCs, a stem cell population within the lamina propria of the gingival tissue, can be isolated from attached and free gingiva, inflamed gingival tissu-es, and from hyperplastic gingiva. Comparatively, they constitute more attractive alternatives to other dental-derived mesenchymal stem cells due to the availability and accessibility of gingival tissues. They have unique immunomodulatory functions and well-documented self-renewal and multipotent differentiation properties. They display positive signals for Stro-1, Oct-4 and SSEA-4 pluripotency-associated markers, with some co-expre-ssing Oct4/Stro-1 or Oct-4/SSEA-4. They should be considered as the best stem cell source for cell-based therapies and regenerative dentistry. The clinical use of GMSCs for regenerative dentistry represents an attrac-tive therapeutic modality. However, numerous biological and technical challenges need to be addressed prior to considering transplantation approaches of GMSCs as clinically realistic therapies for humans.展开更多
Peri-implant epithelial sealing is the first line of defense against external pathogens or stimuli;hence,an essential process to prevent peri-implantitis.Laminin 332(LN332)is the main component of the internal basal l...Peri-implant epithelial sealing is the first line of defense against external pathogens or stimuli;hence,an essential process to prevent peri-implantitis.Laminin 332(LN332)is the main component of the internal basal lamina and participates in peri-implant epithelial sealing by forming hemidesmosomes(HDs)with integrinα6β4.In thiswork,poly(D,L-lactide)(PDLLA)-LN332 composite coating was successfully constructed by a method similar to layer-by-layer assembly,displaying staged LN332 release for as long as 28days.The PDLLA-LN332 composite coating can activate the intracellular PI3K-Akt pathway via binding to cellular integrinα6β4,which can promote adhesion,migration and proliferation of HaCaT cells and further enhance the expression of keratinocyte HD-related molecules,including integrinα6β4,LN332 and plectin.Furthermore,the PDLLA-LN332 composite coating can promote the adhesion,spreading and proliferation of gingival mesenchymal stem cells and accelerate their epithelial differentiation.Therefore,the PDLLA-LN332 composite coating can enhance implant soft tissue sealing,warranting further in vivo study.展开更多
基金supported by the grants from the National Natural Science Foundation of China(30972097)the Key Program of Ministry of Education of China (109148)+2 种基金the Proram for New Century Excellent Talents in University, China (NCET-09-0654)the Scientific Research Program of Shaanxi Province, China (2011K02-06)the China Postdoctoral Science Foundation(20080431253)
文摘Mesenchymal stem cells （MSCs） derived from bone marrow are a well-characterized population of adult stem cells that can be maintained and propagated in culture for a long time with the capacity to form a variety of cell types. This study investigated the characteristics of dairy goat bone marrow MSCs （gMSCs） and their differentiation potential toward germ cells in vitro, and to test their potential in vivo, these ceils were transplanted into seminiferous tubes of endogenous germ cells-depleted mouse models. The results showed that characteristic gMSC lines were established and a small population of gMSCs transdifferentiated into male germ cell-like cells which expressed Stra8 after induction with retinoic acid （RA）, as analysed by reverse transcription-polymerase chain reaction （RT-PCR） and immunofluorescence. Further, we transplanted the gMSCs into endogenous germ cells-depleted mouse models. A variety of analysis demonstrated that gMSCs might differentiate into male germ cells and helped spermatogenesis in endogenous germ cells depleted mouse models at 30 d after transplantation. The gMSCs could be used as a potential source of cells for reproductive studies and a neoadjuvant therapy for the spermatogenesis anomaly. Moreover, these cells may offer a new strategy for male infertility and an alternative approach for production of transgenic animals.
文摘The gingiva, the masticatory portion of the oral mucosa, is excised and discarded frequently during routine dental treatments and following tooth extraction, dental crown lengthening, gingivectomy and periodontal surgeries. Subsequent to excision, healing eventually happens in a short time period after gingival surgery. Clinically, the gingival tissue can be collected very easily and, in the laboratory, it is also very easy to isolate gingival-derived mesenchymal stem cells (GMSCs) from this discarded gingival tissue. GMSCs, a stem cell population within the lamina propria of the gingival tissue, can be isolated from attached and free gingiva, inflamed gingival tissu-es, and from hyperplastic gingiva. Comparatively, they constitute more attractive alternatives to other dental-derived mesenchymal stem cells due to the availability and accessibility of gingival tissues. They have unique immunomodulatory functions and well-documented self-renewal and multipotent differentiation properties. They display positive signals for Stro-1, Oct-4 and SSEA-4 pluripotency-associated markers, with some co-expre-ssing Oct4/Stro-1 or Oct-4/SSEA-4. They should be considered as the best stem cell source for cell-based therapies and regenerative dentistry. The clinical use of GMSCs for regenerative dentistry represents an attrac-tive therapeutic modality. However, numerous biological and technical challenges need to be addressed prior to considering transplantation approaches of GMSCs as clinically realistic therapies for humans.
基金supported by the National Natural Science Foundation of China(No.81970971)Shaanxi Key Research and Development Program(No.2022SF-179).
文摘Peri-implant epithelial sealing is the first line of defense against external pathogens or stimuli;hence,an essential process to prevent peri-implantitis.Laminin 332(LN332)is the main component of the internal basal lamina and participates in peri-implant epithelial sealing by forming hemidesmosomes(HDs)with integrinα6β4.In thiswork,poly(D,L-lactide)(PDLLA)-LN332 composite coating was successfully constructed by a method similar to layer-by-layer assembly,displaying staged LN332 release for as long as 28days.The PDLLA-LN332 composite coating can activate the intracellular PI3K-Akt pathway via binding to cellular integrinα6β4,which can promote adhesion,migration and proliferation of HaCaT cells and further enhance the expression of keratinocyte HD-related molecules,including integrinα6β4,LN332 and plectin.Furthermore,the PDLLA-LN332 composite coating can promote the adhesion,spreading and proliferation of gingival mesenchymal stem cells and accelerate their epithelial differentiation.Therefore,the PDLLA-LN332 composite coating can enhance implant soft tissue sealing,warranting further in vivo study.
