A Glassy Carbon Electrode Modified with Cellulose Nanofibrils from Ammophila arenaria for the Sensitive Detection of L-Trytophan

L-tryptophan is an essential amino acid for human health. Nanofibrillated cellulose (NFC) from marram grass (Ammophila arenaria) extracted from plants harvested in the center of Tunisia was used for the first time for the modification of a glassy carbon electrode (GCE), for the sensitive detection of L-tryptophan (Trp). After spectroscopic and morphological characterization of the extracted NFC, the GC electrode modification was monitored through cyclic voltammetry. The NFC-modified electrode exhibited good analytical performance in detecting Trp with a wide linear range between 7.5 × 10−4 mM and 10−2 mM, a detection limit of 0.2 µM, and a high sensitivity of 140.0 µA∙mM−1. Additionally, the NFC/GCE showed a good reproducibility, good selectivity versus other amino acids, uric acid, ascorbic acid, and good applicability to the detection of Trp in urine samples.

Voltammetric behavior of sedative drug midazolam at glassy carbon electrode in solubilized systems

Redox behavior of midazolam was studied at a glassy carbon electrode in various buffer systems,supporting electrolytes and pH using differential paise,square-wave and cyclic voltammetry.Based on its reduction behavior,a direct differential pulse voltammetric method has been developed and validated for the determination of midazolam in parenteral dosage.Three welldefined peaks were observed in 0.1％ SLS,Britton-Robinson (BR) buffer of pH 2.5.The effect of surfaetants like sodium lauryl sulfate (SLS),cetyl trimethyl ammonium bromide (CTAB) and Tween 20 was studied.Among these surfactants SLS showed significant enhancement in reduction peak.The cathodic peak currents were directly proportional to the concentration of midazolam with correlation coetfficient of 0.99.

Electrooxidation of sulfanilamide and its voltammetric determination in pharmaceutical formulation,human urine and serum on glassy carbon electrode

For the first time, sulfanilamide(SFD) was determined in otologic solution, human urine and serum by electroanalytical techniques on glassy carbon electrode(GCE). The cyclic voltammetry(CV) experiments showed an irreversible oxidation peak at t 1.06 V in 0.1 mol/L BRBS(p H ? 2.0) at 50 m V/s. Different voltammetric scan rates(from 10 to 250 m V/s) suggested that the oxidation of SFD on the GCE was a diffusioncontrolled process. Square-wave voltammetry(SWV) method under optimized conditions showed a linear response to SFD from 5.0 to 74.7 μmol/L(R ? 0.999) with detection and quantification limits of 0.92 and3.10 μmol/L, respectively. The developed SWV method showed better results for detection limit and linear range than the chronoamperometry method. It has been successfully applied to determine SFD concentration in pharmaceutical formulation, human urine and serum samples with recovery close to 100%.

Voltammetric sensor based on cobalt-poly(methionine)-modified glassy carbon electrode for determination of estriol hormone in pharmaceuticals and urine

A voltammetric sensor based on the electropolymerization of cobalt-poly(methionine)(Co-poly(Met)) on a glassy carbon electrode (GCE) was developed and applied for the determination of estriol by differential pulse voltammetry (DPV) for the first time. The electrochemical properties of the Co-poly(Met)/GCE were analysed by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) were used to characterize the polymers on the GCE surface. The deposition of the Co-poly(Met) film on the GCE surface enhanced the sensor electronic transfer. CV studies revealed that estriol exhibits an irreversible oxidation peak at t0.58 V for the Co-poly(Met)/GCE (vs. Ag/AgCl reference electrode) in 0.10 mol/L Britton-Robinson buffer solution (pH=7.0). Different voltammetric scan rates (10-200 mV/s) suggested that the estriol oxidation on the Co-poly(Met)/GCE surface is controlled by adsorption and diffusion processes. Based on the optimized DPV conditions, the linear responses for estriol quantification were from 0.596 μmol/L to 4.76 μmol/L (R2 =0.996) and from 5.66 μmol/L to 9.90 μmol/L (R2 =0.994) with a limit of detection (LOD) of 0.0340 μmol/L and a limit of quantification (LOQ) of 0.113 μmol/L. The DPV-Co-poly(Met)/GCE method provided good intra-day and inter-day repeatability with RSD values lower than 5%. Also, no interference of real sample matrices was observed on the estriol voltammetric response, making the DPV-Copoly( Met)/GCE highly selective for estriol. The accuracy test showed that the estriol recovery was in the ranges 96.7%-103% and 98.7%-102% for pharmaceutical tablets and human urine, respectively. The estriol quantification in pharmaceutical tablets performed by the Co-poly(Met)/GCE-assisted DPV method was comparable to the official analytical protocols.

Cyclic Voltammetry Determination of Epinephrine with a Nano-gold Modified Glassy Carbon Electrode in the Presence of High Concentration Ascorbic Acid

Nano-gold (NG) modified glassy carbon electrodes (GCEs) were used for determination of epinephrine (EP) in the presence of high concentration ascorbic acid (AA) by cyclic voltammetry (CV). This modified electrode can not only catalytically oxidize EP and AA, but also separate the catalytic peak potentials of EP and AA by about 183.5 mV. In pH = 7.0 ogisogate byffer solution, the linear range of epinephrine was 5 106 ~ 1 ?10-4 mol/L.

Direct Electrochemistry and Electrocatalysis of Hemoglobin at PAMAM Dendrimer-MWNTs-Au Nanoparticles Composite Film Modified Glassy Carbon Electrode

The direct electron transfer of hemoglobin at the PAMAM-MWNTs-AuNPs composite film modified glassy carbon electrode was studied. In a phosphate buffer solution（PBS, pH=7.0）, the formal potential（E^0） of Hb was -0.105 V versus SCE, the electron transfer rate constant was 4.66 s-1. E^0＇ of Hb at the modified electrode was linearly varied in a pH range of 5.0-8.0 with a slope of-49.2 mV/pH. The Hb/PAMAM-MWNTs-AuNPs/GCE gave an excellent electrocatalytic response to the reduction of hydrogen peroxide. The catalytic current increased linearly with H2O2 concentration in a range of 1.0× 10^-6 to 2.2× 10^-3 mol/L. The detection limit was 2.0× 10^-7 mol/L at a signal to noise ratio of 3. The Michaelis-Menten constant（Km^app） was 2.95 mmol/L.

Glassy carbon electrode modified with multi-walled carbon nanotubes sensor for the quantihication of antihistamine drug pheniramine in solubilized systems

A sensitive electroanalytical method for quantification of pheniramine in pharmaceutical formulation has been investigated on the basis of the enhanced electrochemical response at glassy carbon electrode modified with multi-walled carbon nanotubes in the presence of sodium lauryl sulfate.The experimental results suggest that the phcniramine in anionic surfactant solution exhibits electrocatalytic effect resulting in a marked enhancement of the peak current response.Peak current response is linearly dependent on the concentration of pheniramine in the range 200-1500 μg/mL with correlation coefficient 0.9987.The limit of detection is 58.31 μg/m L.The modified electrode shows good sensitivity and repeatability.

Voltammetric Response of Epinephrine at Carbon Nanotube Modified Glassy Carbon Electrode and Activated Glassy Carbon Electrode

The electrochemical behavior of epinephrine at activated glassy carbon electrode and carbon nanotube-coated glassy carbon electrode was studied. Epinephrine could exhibit an anodic peak at about 0.2 V （vs. SCE） at bare glassy carbon electrode, but it was very small. However, when the electrode was activated at certain potential （i.e. 1.9 V） or modified with carbon nanotube, the peak became more sensitive, resulting from the increase in electrode area in addition to the electrostatic attraction. Under the selected conditions, the anodic peak current was linear to epinephrine concentration in the range of 3.3×10^-7 - 1.1×10^-5 mol/L at activated glassy carbon electrode and in the range of 1.0×10^-5 - 5.0×10^-5 mol/L at carbon nanotube-coated electrode. The correlation coefficients were 0.998 and 0.997, respectively. The determination limit was 1.0×10^-7 mol/L. The two electrodes have been successfully applied for the determination of epinephrine in adrenaline hydrochloride injection with recovery of 95%-104%.

Voltammetric Response and Electrocatalysis of Glassy Carbon Electrode Modified with Monolayer Nickel Hydroxide

The glassy carbon (GC) electrode modified with a monolayer nickel hydroxide (GC/Ni(OH) 2) was prepared by immersion of GC substrate in 1.0×10 -3 mol/L NiSO 4 solution, and then cyclic voltammetric scanning in 0.20 mol/L KOH. Similarly, GC/Co(OH) 2 electrode was prepared too. The experiments showed that the voltammetric behavior of GC/Ni(OH) 2 electrode in 0.20 mol/L KOH is more stable than that of GC/ Co(OH) 2. It was found that the GC/Ni(OH) 2 electrode acts as an effective electrocatalysis for the oxidation of hydrazine.

Voltammetric quantitation of nitazoxanide by glassy carbon electrode

The present study reports voltammetric reduction of nitazoxanide in Britton-Robinson （B-R） buffer by cyclic and square-wave voltammetry at glassy carbon electrode. A versatile fully validated voltammetric method for quantitative determination of nitazoxanide in pharmaceutical formulation has been proposed. A squrewave peak current was linear over the nitazoxanide concentration in the range of 20-140 ~tg/mL. The limit of detection （LOD） and limit of quantification （LOQ） was calculated to be 5.23 la~/mL and 17.45 la~/mL, respectively.

Voltammetric Detection of Nanomolar Levels of Hypoxanthine in the Presence of Copper(Ⅱ) at Glassy Carbon Electrode

Nanomolar levels of the hypoxanthine in NaOH electrolyte cantaining copper(Ⅱ) can be determined by anodic stripping voltammetry at a glassy carbon electrode. In the present article hypoxanthine Cu + is shown to be adsorbed on the electrode surface in the presence of an excess of copper(Ⅱ). After accumulation period, hypoxanthine Cu + was stripped from the electrode surface and the anodic current coming near to the oxidation of Cu(Ⅰ) to Cu(Ⅱ) was measured. A linear calibration curve in the range of 5 nmol/L 1.5 mmol/L hypoxanthine, with a detection limit of 0.5 nmol/L hypoxanthine were obtained.

A Functionalized Multi-walled Carbon Nanotube and Gold Nanoparticle Composite-modified Glassy Carbon Electrode for the Detection of Enterovirus 71

Hand, foot, and mouth disease (HFMD) is a common infectious disease in children, occurring primarily in preschool children[1_3] with infants under three years old being gen erally susceptible. The disease is caused by various enteroviruses, among which EV71 and Coxsackievirus A group 16 (Cox A16) are the most comm on ⑷.According to in formation released by the Chinese Center for Disease Control and Prevention on June 8, 2016, EV71 infection- related HFMD has been prevalent among infants and young children in China since 2007, with a high incidenee and many deaths.

The Electrochemical Behavior and the Determination of Bavistin on Glassy Carbon Electrode

In this paper, the electrochendcal behavior of bavistin (MBC) on glassy carbon electrode is reported. In a base solution of pH=9.0 NH3-NH4Cl, a sensitive anodic peak was found by cyclic voltammetry. Differential pulse stripping voltanunetry was applied for determing MBC in grains. The detection limit is 4×10-8mo/L.The recovery is from 91.3% to 95.7%. The method has advantages of simplicity and high sensitivity.

DIRECT ELECTROCHEMICAL BEHAVIOUR OF CYTOCHROME C AT A BARE GLASSY CARBON ELECTRODE

A reversible electron transfer between horse heart cytochrome c and a bare glassy carbon electrode was found and the dependence of direct electrochemical behaviotw on the electrode surface state was discussed.

Electrochemical behavior of paclitaxel and its determination at glassy carbon electrode

The electrochemical behavior of paclitaxel drug was studied at a glassy carbon electrode in phosphate buffer solutions using cyclic and differential-pulse voltammetric techniques.The oxidation process was shown to be irreversible over the pH range(3.0e10.4)and was diffusion controlled.Effects of anodic peak potential(E_(p)),anodic peak current(Ipa),scan rate,pH,heterogeneous rate constant(k^(0)),etc have been discussed.A possible electrooxidation mechanism was proposed.An analytical method was developed for the determination of paclitaxel in phosphate buffer solution at pH¼7.0 as a supporting electrolyte.The anodic peak current varied linearly with paclitaxel concentration in the range 1.0×10^-(6)M to 1.0×10^-(5)M with a limit of detection(LOD)of 1.23×10^(-8)M and limit of quantification(LOQ)of 4.10×10^(-8)M.The proposed method was successfully applied to the determination of paclitaxel in pure and real samples.

Electrochemical Behavior of Chalcone at a Glassy Carbon Electrode and Its Analytical Applications

A simple and rapid method was developed using cyclic, differential pulse and square wave voltammetric techniques for the determination of trace-level chalcone at a glassy carbon electrode. Chalcone could produce two anodic peaks at about 0.514 V and 1.478 V and a cathodic peak at about -0.689 V. The differential pulse voltammerty presents a good linear response as compared to square wave voltammetry in the range of 0.2 - 10 μM with a detection limit of 0.18 μM. The proposed method was used successfully for its quantitative determination in spiked human plasma and urine as real samples.

Electro Analytical Determination of Di Hydroxybenzene Isomers Using Glassy Carbon Electrode

In this study, the electrochemical oxidation of CT （catechol）, HQ （hydroquinone） and RS （resorcinol） was investigated using cyclic and linear sweep voltammetries at GCE （glassy carbon electrode）. The GCE showed an excellent electro activity and reversibility towards the oxidation of these isomers at different conditions. HQ and CT showed one defined oxidation peak and one defined reduction peak while RS showed one defined oxidation peak. These isomers were determined also in their binary and tertiary mixtures. The calibration curves for CT, HQ and RS were obtained in the ranges of 5 × 10-6 to 1 × 10-3 mol.dm-3, 5 × 10-6 to 5 × 10-4mol.dm-3 and 1 × 10-5 to 1× 10-3 mol.dm-3, respectively. The detection limits were 9 ×10-7, 3 × 10-7, 6 × 10-6 mol.dm3 for CT, HQ and RS, respectively. At the optimal experimental conditions, these isomers were determined in different water samples. Also, the removal of catechol from aqueous solution by adsorption on activated charcoal and alumina was studied. After 24 h, 88.7% and 65.9% of catechol was removed using charcoal and alumina, respectively.

Use of Graphene and Cucurbit[7]uril Electrodes for the Determination of Amantadine in Biological Fluids

A differential pulse voltammetry (DPV) method for amantadine (AT) determination is developed. To this end, all the chemical and instrumental variables affecting the determination of amantadine are optimized. These studies have used three types of glassy-carbon electrode, first electrode which has not undergone surface modification or coating, to then modify the working electrode surface with two kinds of suspensions: graphene and graphene-cucurbit[7]uril (CB[7]). From studies of the mechanisms governing the electrochemical response of amantadine, it was concluded that it is an electrochemically system with a diffusive reduction phenomenon. Under optimal conditions and with the appropriate electrode modification, we proceed to study the relation between the peak intensity with the analyte concentration. Thus, we find that when the electrode surface is covered with graphene-CB[7], two linear sections are obtained, the first one in the concentration range of between 0.05 μg·mL﹣1 and 0.75 μg·mL﹣1;and the second one between 1.00 μg·mL﹣1 and 6.00 μg·mL﹣1, with Er (%) = 87 and R.S.D. = 0.94% (n = 10 at 0.5 μg·mL﹣1 level). The minimum detectable amount was 15 ng·mL﹣1 while a concentration of 44 ng·mL﹣1 was calculated as determination limit. The optimized method was applied to the determination of amantadine in biological fluids.

羧基化多壁碳纳米管修饰电极测定左氧氟沙星

探究了左氧氟沙星在羧基化多壁碳纳米管(c-MWCNTs)修饰电极上的电化学行为,并采用循环伏安法对左氧氟沙星含量进行了测定.结果表明,c-MWCNTs修饰电极对左氧氟沙星有明显的电催化作用;在pH值为5.0的NaH_(2)PO_(4)Na_(2)HPO_(4)缓冲溶液中,左氧氟沙星溶液的氧化峰电流与浓度在1×10^(-5)~4×10^(-4)mol/L范围内呈良好线性关系,相关系数为0.998,检出限为2.87×10^(-6)mol/L;在最优测试条件下,左氧氟沙星片剂的加标回收率为98.7%~103.0%,相对标准偏差为1.31%~3.99%(n=5);羧基化多壁碳纳米管修饰玻碳电极(c-MWCNTs/GCE)对左氧氟沙星有较好的电化学响应,灵敏度高,可用于药品中左氧氟沙星测定.

马来酸氯苯那敏在GCE和MWCNT/GCE上的电催化氧化及电分析方法

目的:研究马来酸氯苯那敏(chlorphenamine maleate,CPM)在玻碳电极(GCE)及多壁碳纳米管修饰玻碳电极(MWCNT/GCE)上的电化学行为、电化学动力学和电化学分析厅法。方法:采用循环伏安法(CV)、计时库仑法(CC)、计时电流法(CA)、线性扫描伏安法(LSV)。结果:与 GCE 相比,CPM 在 MWCNT/GCE 上氧化峰电流明显增大,氧化峰电位负移90 mV,表明MWCNT/GCE 对 CPM 电化学氧化具有良好的催化作用。同时研究了实验条件对 CPM 电化学行为的影响。CPM 住 GCE 及MWCNT/GCE 上在扫描速度10～1000 mV·s^(-1)范围内氧化峰电流(I_(pa))与扫描速度平方根(v^(1/2))成正比,其电化学氧化反应是-受扩散控制的电极过程。研究了 CPM 在 GCE 及 MWCNT/GCE 上氧化峰电流与浓度分别在5.0×10^(-5)～1.5×10^(-3)mol·L^(-1)范围内呈良好的线性关系,检出限分别为1.0×10^(-5),5.0×10^(-6)mol·L^(-1)。RSD 在0.56%～1.8%之间,加样回收率在99,5%～103.8%之间。同时分别测定了 CPM 在 GCE 及 MWCNT/GCE 上的电极过程动力学参数:电荷转移系数(α)分别为0.77,0.90;扩散系数(D)分别为5.60×10^(-8),6.48×10^(-8)cm^2·s^(-1);电极反应速率常数(k_j)分别为2.02×10^(-3),5.45×10^(-3)s^(-1)。结论:该方法灵敏度高,操作简单,可用于 CPM 的电化学测定。