Effects of high dose glucocorticoid on expression and mRNA transcription of corticotropin releasing hormone in hypothalamus paraventricular nucleus of rats

Objective： To explore the effect of high dose of glucocorticoid （GC） on the synthesis of corticotropin releasing hormone （CRH） and transcription of its mRNA in hypothalamus paraventricular nuclei （PVN） in order to investigate its difference with that of traditional GC effects and to add a new possible explanation to the mechanism of clinical applications of high dose of GC. Methods： A total of 60 rats were divided into 5 groups： blank control, 10^-6 mol/L dexamethasone （DEX） group, 10^-9 mol/L DEX group, 0.9% saline group and GR blocking group （10^-2mol/L RU486）. All agents were administrated through the femoral vein. CRH protein expression was measured by immunohistochemistry and laser confocal scanning microscopy （LCSM）; CRH mRNA level was explored by in situ hybridization. Results： 10^-6 mol/L DEX made CRH mRNA transcripted after 20 min and its protein expressed in PVN after 30 min, while normal level of DEX and 0.9% saline could not. If GR was blocked in advance, the effect of high dose of DEX disappeared. Conclusion ： High dose of GC can have CRH increased in PVN, which differs to the effect of traditional GC. And mGRH may play an important role in the effect of high dose of GC but not classic iGR.

Effects of glucocorticoid combined with antibiotics on serum infection indexes, acute phase proteins and stress hormones in patients with severe pneumonia

Objective: To study the effects of glucocorticoid combined with antibiotics on serum infection indexes, acute phase proteins and stress hormones in patients with severe pneumonia. Methods: a total of 80 patients with severe pneumonia who were hospitalized between August 2014 and January 2017 were retrospectively analyzed and divided into the routine treatment group (n=46) who received conventional antibiotic therapy and the combined treatment group (n=34) who received glucocorticoid combined with antibiotic therapy, and the differences in infection indexes, acute proteins and stress hormones were compared between the two groups of patients before and after treatment. Results: The differences in serum levels of infection indexes, acute phase proteins and stress hormones were not statistically significant between the two groups before treatment. After 1 week of treatment, serum infection indexes CRP and PCT levels of observation group were lower than those of control group;serum acute phase proteins α1-AT, α1-AG and CER levels were lower than those of control group;serum stress hormones Cor, AngⅠ and AngⅡ levels were lower than those of control group. Conclusion:Glucocorticoid combined with antibiotics can effectively inhibit systemic infection and stress and optimize the illness in patients with severe pneumonia.

Occurrences of Glucocorticoids in Aquatic Environment and Their Removal during Wastewater Treatment

Glucocorticoids(GCs) are a group of endocrine-disrupting compounds(EDCs) frequently prescribed against various medical conditions.Recently,GCs have been shown to be effective in managing septic shock in patients infected with the 2019 novel coronavirus(COVID-19).Due to colossal consumption and potential risks to aquatic organisms,GCs have immensely attracted the focus of the scientific research community as a water pollutant.Therefore,the aim of this paper is to review the current knowledge on the occurrence of various GCs in the aquatic environment and their removal during wastewater treatment.A variety of GCs are ubiquitous in surface water,hospital wastewater,and sewage water worldwide.And the minimum concentration in volume is below 0.01 ng/L,and the maximum one is 10 000 ng/L,and enter the environment through hospital and urban wastewater discharging.Compared with natural GCs,higher risks to aquatic environments could be induced by synthetic GCs.The current activated sludge processes used in wastewater treatment plants(WWTPs) are not fully effective in eliminating GCs,some of which may further increase the risk of GC in the environment.In comparison with the aerobic process in WWTPs,the anaerobic and anoxic processes were found to be more efficient for GC degradation.Of the studied GCs,fluticasone propionate,clobetasol propionate,fluocinolone acetonide,and triamcinolone acetonide need more attention due to their low removal efficiencies and strong toxicity.Among the advanced treatment processes,reverse osmosis,ultraviolet irradiation,CaO_(2),and plasma could achieve significant GC activity removal while micro/ultra-filtration,chlorination,and ozonation were less efficient.

GC-MS联用技术检测水性化妆品中性激素成分的方法研究

[目的 ]研究建立水性化妆品中性激素成分的测定方法。 [方法 ]采用气相色谱 质谱 (GC MS)联用技术同时分析水性化妆品中的 7种性激素成分。样品经提取、去脂、使用C18固相提取小柱净化 ,目标物用七氟丁酸酐衍生化 ,用GC MS SIM分析。 [结果 ] 7种性激素的平均回收率在 75 %～ 10 9.7%之间 ,方法检出限为 0 .0 2 5～ 0 .0 5 0 μg/ml ,方法相对标准偏差为 8.4%～ 16.3 %。 [结论

生物体内5种游离生物激素的负化学源GC-MS连续分析研究

生物激素对生物体的生长发育和繁殖有着重要的生物学意义.已经有大量生物体体液或组织中的激素测定报道[1～4],但大多是针对某种激素或是一类激素的研究,还未见将5种游离生物激素(肾上腺素、去甲肾上腺素、多巴胺、17β-雌二醇和孕酮)两类激素一次性连续测定的报道.……

林蛙油中激素类成分的GC-MS分析

目的研究林蛙油中激素类成分的种类及含量。方法利用气相色谱-质谱联用(GC-MS)的方法,对林蛙油中激素类成分进行测定。结果经过初步分析,林蛙油中激素类成分为乙酸雄甾酮(0.33%)、前列腺素(0.35%)、美屈孕酮(0.52%)、胆固醇(23.94%)及雌二醇(0.87%)。结论本实验方法稳定、有效,适用于林蛙油中激素类成分的定性定量分析。

小鼠卵泡颗粒细胞在生殖衰老过程中基因、蛋白表达变化研究

为研究生殖衰老对小鼠卵泡颗粒细胞(Granulosa cells,GCs)发育的影响,本试验对课题组前期的转录组与蛋白质组数据进行了分析,这些数据来自于6周龄(年轻组)、10月龄(衰老组)的健康雌性昆明(KM)小鼠。结果显示,转录组数据与蛋白组数据得到的差异基因与差异蛋白的符合程度并不高,共筛选出95个基因和蛋白同时在转录水平和蛋白水平发生上调,有22个基因在转录水平和蛋白水平发生下调,采用qPCR与Westernblot方法对其中的相关基因的表达水平进行了验证。结果表明,与年轻组相比,衰老组卵泡颗粒细胞的凋亡相关基因Ndrg1和Sting1表达显著上升(P<0.05),抗氧化相关基因Gpx7、生长因子相关基因Gdf9、Bmpr1b与激素合成、分泌相关基因Esr2、Lhcgr表达显著下降(P<0.05)。综上说明机体发生生殖衰老后卵泡颗粒细胞的凋亡水平上升,抗氧化功能下降,激素作用途径受损。

不同剂量糖皮质激素替代治疗对腺垂体功能减退症患者激素暴露的影响

目的探讨不同剂量糖皮质激素替代治疗对腺垂体功能减退症患者激素暴露的影响。方法回顾性分析2018年10月至2021年10月间于濮阳市中医医院就诊的72例腺垂体功能减退症患者临床资料,根据不同用药剂量分为低剂量组(27例)、正常剂量组(24例)和高剂量组(21例)。比较3组患者糖化血红蛋白、空腹血糖、钾离子、钠离子、尿酸、舒张压、收缩压、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇及总胆固醇各项临床指标数据;将3组糖尿病、高血压病、血脂异常、慢性胃炎、非酒精性脂肪肝、冠心病、向心性肥胖、精神症状发生情况进行比较;比较3组患者治疗前后的躯体功能、生理功能、健康状态、认知功能、社会功能、角色功能。结果3组患者糖化血红蛋白、空腹血糖、钾离子、钠离子、尿酸水平比较均差异无统计学意义(P均>0.05);高剂量组患者舒张压、收缩压及低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、总胆固醇水平均高于低剂量组与正常剂量组(P均<0.05)。高剂量组发生高血压病、血脂异常、慢性胃炎、非酒精性脂肪肝病、冠心病、向心性肥胖、精神症状患者的占比高于低剂量组与正常剂量组(P均<0.05)。3组患者治疗后生存质量均有所提升,低剂量组患者健康状态优于正常剂量组与高剂量组患者(P<0.05)。3组躯体功能、生理功能、认知功能、社会功能及角色功能比较均差异无统计学意义(P均>0.05)。结论腺垂体功能减退症患者采用不同剂量糖皮质激素的个性化代替治疗对其激素暴露的影响程度也有所不同,治疗过程中需监测患者血脂、血糖变化,低剂量的糖皮质激素替代疗法可有效降低患者激素暴露的风险,临床可根据患者不同症状进行个体化给药治疗,提高患者生存质量。

内源性甾体激素GC/MS分析中酮基衍生规律的研究

本实验以甲氧胺(MOX)和N-甲基-N-(三甲基硅烷)三氟乙酰胺(MSTFA)为衍生化试剂,采用肟化-硅烷化方法对12种内源性甾体激素进行衍生,通过GC/MS解析鉴定衍生产物结构,研究甾体激素的衍生规律。结果表明,肟化-硅烷化反应可以提高甾体激素的信号,但孕酮(P)、雄烯二酮(ASD)、雌酮(E1)、睾酮(T)、皮质甾酮(B)、可的松(E)受共轭结构影响,产生多种同分异构体。[M-31]^+和[M-15]^+,[M-90]^+和[M-72]^+可分别作为肟化和硅烷化反应的鉴定依据。在80℃,加热40 min,酮基与MOX的摩尔比为1∶20是肟化反应的最佳条件。采用单反应离子检测(SRM)模式,衍生后甾体激素的检测灵敏度可达1μg/L,线性范围为1~200μg/L。该方法可为相关激素类成分的分析提供检测技术支持。

Expression of hippocampal corticosteroid receptors,as well as corticotrophin-releasing hormone and vasopressin in the hypothalamic paraventricular nucleus,in fornix transected rats

BACKGROUND： The hippocampus regulates the hypothalamic-pituitary-adrenal axis through negative feedback. The hypothalamic paraventricular nucleus receives neuronal input from the hippocampus via the fomix, OBJECTIVE： To explore whether the negative feedback effect of the hippocampus on the hypothalamic-pituitary-adrenal axis is contributed to the inhibitory effect of mineralocorticoid receptor （MR） and glucocorticoid receptor （GR） in the hippocampus on the paraventricular nucleus via the fornix. DESIGN, TIME AND SETTING： Randomized, controlled, animal experiment. The study was performed at the Department of Histology and Embryology, China Medical University between September 2006 and September 2008. MATERIALS： Rabbit anti-rat anti-MR and rabbit anti-rat anti-GR antibodies were purchased from Santa Cruz Biotechnology, USA. Rabbit anti-rat anti-corticotrophin releasing hormone （CRH） and rabbit anti-rat anti-arginine vasopressin antibodies were purchased from Wuhan Boster. METHODS： A total of 90 male, Wistar rats were randomly divided into model and sham-surgery groups （n = 45）. Fornix transection was performed in the model group, while the sham-surgery group underwent surgery, but no fornix transection. MAIN OUTCOME MEASURES： Immunohistochemistry was used to examine MR and GR expression in the hippocampus, as well as CRH and anti-arginine vasopressin in the paraventricular nucleus. Western blot was used to measure alterations in MR, GR, and CRH protein expression following fomix transection. RESULTS： Compared with the sham-surgery group, there were no obvious changes in MR and GR expression in the hippocampus, or CRH and anti-arginine vasopressin expression in the paraventdcular nucleus within 4 days of fornix transection. However, after 7-10 days, significantly decreased MR and GR expression in the hippocampus, and increased CRH and anti-arginine vasopmssin expression in the paraventricular nucleus were observed （P 〈 0.05-0.01）. CONCLUSION： Negative feedback from the hippocampus on the hypothalamic-pituitary-adrenal axis might be mediated through the fornix, and the corticosterene actions mediated by hippocampal corticosteroid receptors indirectly modulated the hypothalamic-pituitary-adrenal axis.

Functional interactions between steroid hormones and neurotrophin BDNF

Brain-derived neurotrophic factor(BDNF),a critical neurotrophin,regulates many neuronal aspects including cell differentiation,cell survival,neurotransmission,and synaptic plasticity in the central nervous system(CNS) .Though BDNF has two types of receptors,high affinity tropomyosin-related kinase(Trk) B and low affinity p75 receptors,BDNF positively exerts its biological effects on neurons via activation of TrkB and of resultant intracellular signaling cascades including mitogenactivated protein kinase/extracellular signal-regulated protein kinase,phospholipase Cγ,and phosphoinositide 3-kinase pathways.Notably,it is possible that alteration in the expression and/or function of BDNF in the CNS is involved in the pathophysiology of various brain diseases such as stroke,Parkinson's disease,Alzheimer's disease,and mental disorders.On the other hand,glucocorticoids,stress-induced steroid hormones,also putatively contribute to the pathophysiology of depression.Interestingly,in addition to the reduction in BDNF levels due to increased glucocorticoid exposure,current reports demonstrate possible interactions between glucocorticoids and BDNF-mediated neuronal functions. Other steroid hormones,such as estrogen,are involved in not only sexual differentiation in the brain,but also numerous neuronal events including cell survival and synaptic plasticity.Furthermore,it is well known that estrogen plays a role in the pathophysiology of Parkinson's disease,Alzheimer's disease,and mental illness,while serving to regulate BDNF expression and/or function.Here,we present a broad overview of the current knowledge concerning the association between BDNF expression/function and steroid hormones(glucocorticoids and estrogen).

高血压脑出血患者急性期血中TNF-α、IL-1β、ACTH、GC含量测定及其相互作用机制的研究

目的测定高血压脑出血患者急性期血中肿瘤坏死因子-α(TNF-α)、白细胞介素-1(IL-1β)、促肾上腺皮质激素(ACTH)、皮质醇(GC)的含量。方法采用ELISA法测定32例脑出血患者晨间7￣8时和夜间23￣24时外周血TNF-α、IL-1β、ACTH、GC的含量。结果脑出血组血中4种物质含量均高于正常对照组,TNF-α和IL-1β与ACTH、GC含量变化有正相关关系。对照组晨间4种物质含量均高于夜间水平,脑出血组晨间的含量均低于夜间。结论TNF-α、和IL-1β、ACTH、GC之间存在相互作用机制,构成神经内分泌和免疫调节网络,参与脑出血急性期的应激反应。

Clinical and pathobiological heterogeneity of asthma—Mechanisms of severe and glucocorticoid-resistant asthma

It is increasingly recognized that asthma represents a syndrome, and there is clinical and pathobiological heterogeneity. Many genes are reported to be associated with asthma, and may be involved in the disease heterogeneity. Diverse cells, such as T helper 1 (Th1)-cells, Th2-cells, Th17-cells, airway epithelial cells, and innate and adaptive immunity associated cells, contribute to the pathobiology of asthma independently of each other or they can also coexist and interact. Although, generally, Th2 immunity is important in most asthma endotypes, non- Th2-driven inflammation tends to be difficult to manage. Recently, increased attention has been focused on severe asthma and glucocorticoid (GC)-resistant (GC-R) asthma, in which diverse inflammatory processes may be involved. Treatment approaches should take into account pathological differences.

Effects of thyrotropin-releasing hormone on severe head injury:A preliminary clinical trial

Objective: To evaluate the effects of thyorotropin-releasing hormone (TRH ) on severe head injury.Methods: Eighty--seven severely head injured patients with a Glasgow Coma Scale (GCS ) score of & or less wererandomized into TRH--treated and saline control groups. In TRH treated group. the treatment was started with abolus injection of 0. 2 mg/kg followed by continuous infusion for 2 hours at 0. 2 mg/kg/h. Such treatment wasgiven once a day for 4 times. The patients in control group were given the equivalent normal saline with the samemethod. Results: TRH, administered intravenously after head injury. promoted the recovery of consciousness andGCS score, alleviated the traumatic brain edema, controlled and lowered the intracranial pressure. decreased thelevel of lipid superoxides, decreased the mortality rate. and improved the life quality of the survivals. Nocomplications or adverse and toxic effects were noted during the course of TRH treatment. Conclusion: TRH hasbeneficial effects on patients with severe head injury.

分散固相萃取-气相色谱-串联质谱法同时测定稻谷中三种烯虫酯类保幼激素类似物残留

以分散固相萃取(DSPE)作为样品前处理手段,采用气相色谱-串联质谱(GC-MS/MS)检测技术,建立了适用于稻谷中3种烯虫酯类保幼激素类似物残留量同时测定的方法。样品使用1%乙酸-乙腈提取,经1.2 g无水硫酸镁、0.4 g N-丙基乙二胺(PSA)、0.2 g十八烷基硅烷键合硅胶(C18)分散萃取净化,采用DB-1 701(30 m×0.25 mm,0.25μm)毛细管柱分离,动态多重反应监测模式下(DMRM)测定,基质匹配标准曲线内标法定量。结果表明,3种保幼激素类似物在0.01~0.32 mg/L质量浓度范围内线性关系良好,相关系数均大于0.999,烯虫乙酯定量限为0.005 mg/kg,烯虫炔酯和烯虫酯定量限为0.01 mg/kg,3个添加水平(0.02,0.1,0.2 mg/kg)回收率分别在86.0%~107.3%之间,相对标准偏差为1.0%~5.7%(RSDs,n=6)。应用该方法对市售25份稻谷样品进行检测,3种保幼激素类似物均未检出。该方法具有操作简便、灵敏度高,准确度好等优点,适用于稻谷中3种烯虫酯类保幼激素类似物残留量的同时测定。

CRHR1基因rs242941位点多态性与哮喘儿童ICS 长期安全用药的关系

目的研究促肾上腺皮质激素释放激素受体1(CRHR1)基因rs242941位点的多态性与哮喘儿童吸入性糖皮质激素(ICS)长期安全用药的关系。方法选取2018年10月至2020年10月该院收治的100例哮喘儿童,均接受ICS治疗及常规对症治疗。采用TaqMan法检测CRHR1基因rs242941位点多态性,根据基因型分为GG、GT+TT,比较不同基因型患儿的肺功能、哮喘控制测试评分及不良反应发生情况。采用多因素Logistic回归分析影响哮喘儿童ICS安全用药的危险因素。结果CRHR1基因rs242941位点存在3种基因型:GG(72例)、GT(20例)、TT(8例),G和T等位基因频率分别为82%和18%。治疗12周后不同基因型患儿的哮喘控制测试评分、第1秒用力呼气容积(FEV1)、FEV1/用力肺活量(FVC)比值与治疗前比较均明显提升,其中GG基因型患儿改善情况优于GT+TT基因型,GG基因型患儿的症状及体征完全缓解时间明显短于GT+TT基因型,差异有统计学意义(P<0.05)。GG基因型不良反应发生率低于GT+TT基因型,差异有统计学意义(P<0.05)。单因素、多因素Logistic回归分析显示,rs242941位点基因型多态性是影响患者用药安全的独立危险因素(P<0.05)。结论CRHR1基因rs242941位点多态性与哮喘儿童ICS的疗效及长期用药安全性密切关联,其中TT基因型患儿具有更高的ICS不良反应发生风险。

糖皮质激素替代剂量对腺垂体功能减退症患者激素暴露的影响

目的探讨糖皮质激素替代剂量(小剂量、生理剂量及超生理剂量)对腺垂体功能减退症(HP)患者激素暴露的影响。方法收集2010年1月至2020年12月在湖南师范大学附属湘东医院和湖南师范大学附属第一医院确诊的192例服用稳定剂量糖皮质激素(GC)替代治疗至少1年的HP患者的临床资料进行回顾性分析,根据HP患者服用GC剂量分为小剂量组(HCeq<20 mg/d)(37例)、生理剂量组(HCeq=20 mg/d)(53例)、超生理剂量组(HCeq≥30 mg/d)(102例),比较各组间激素暴露风险差异及危险因素。结果与生理剂量组、超生理剂量组对比,小剂量组收缩压(SBP)、舒张压(DBP)、空腹血糖(FBG)、尿酸(UA)、天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、总胆固醇(TC)、三酰甘油(TG)、第1天糖皮质激素剂量(GC_( 1))、第2天糖皮质激素剂量(GC_( 2))、第3天糖皮质激素剂量(GC _(3))偏低,高密度脂蛋白胆固醇(HDL-C)更高,差异均有统计学意义(P<0.05);与生理剂量组、超生理剂量组对比,小剂量组向心性肥胖、非酒精性脂肪肝病(NAFLD)、血脂异常、高血压病、冠心病、低骨量、骨质疏松、慢性胃炎、精神症状发生率更低,差异均有统计学意义(P<0.05);二元Logistic回归分析显示,以小剂量组为参照,生理及超生理剂量GC是向心性肥胖、血脂异常、高血压病、骨质疏松的危险因素(P<0.05);超生理剂量GC是NAFLD、冠心病、慢性胃炎、低骨量的危险因素(P<0.05);GC _(1)、GC_( 2)是发生精神症状的危险因素(P<0.05);生活质量评估:与生理剂量组、超生理剂量组相比,小剂量组在生理职能等4个维度得分较高,差异有统计学意义(P<0.05)。结论不同剂量GC替代治疗对HP患者激素暴露的影响程度有所不同,治疗HP患者,强调个性化糖皮质激素替代,其中小剂量GC替代治疗在改善生活质量的同时可降低激素暴露风险。

分子印迹固相萃取-液液微萃取-气相色谱法测定鱼样品中痕量环境激素

以题示方法测定鱼样品中痕量的双酚A(BPA)、苯胺(AN)、二苯胺(DPA)、4-氯-二甲基苯胺(CMAN)、3-氨基酚(3-AP)、苯酚(PH)等6种环境激素。以双酚A&苯胺双模板磁性分子印迹聚合物(D-MIP)为固相萃取剂,甲醇及乙酸混合溶液为洗脱剂,萃取鱼样品中6种环境激素;乙腈为分散剂,正辛醇为萃取剂,液液微萃取洗脱液,等体积甲醇稀释萃取液后进行气相色谱检测。结果表明,鱼样品经处理后基质干扰已消除,6种环境激素能有效分离。对固相萃取及液液微萃取进行了条件优化,优化结果为:洗脱剂甲醇与乙酸用量为3 mL(V甲醇∶V乙酸=8∶2),洗脱时间2 min,D-MIP用量30 mg,固相萃取时间10 min,鱼样品溶液体积50 mL;液液微萃取剂为正辛醇,正辛醇用量90μL,乙腈为分散剂,乙腈用量0.5m L,5 mL10%NaCl溶液,pH=7。在优化的条件下,6种环境激素的标准曲线的线性范围为0.1~50μg/L,检出限(3S/N)为0.1~0.5 ng/L;对空白鲫鱼肉样品进行2个浓度水平的加标回收试验,6种环境激素的回收率为86.1%~92.5%,测定值的相对标准偏差(n=3)均小于1.8%;方法用于6种鱼样品的分析,只有鲫鱼鳃含有BPA 0.1μg/kg,其他均未检出。此方法将为鱼类中环境激素的含量检测提供方法依据。

他克莫司治疗慢性炎性脱髓鞘性多发性神经根神经病的疗效

目的 对比他克莫司与糖皮质激素用于慢性炎性脱髓鞘性多发性神经根神经病(CIDP)维持治疗的临床疗效。方法 收集2019-05—2021-10在重庆医科大学附属第一医院神经内科住院治疗的CIDP患者32例,分为他克莫司治疗组(观察组)与激素治疗组(对照组)。通过对2组患者维持治疗前后反映疾病严重程度的专科量表[包括评估损伤的英国医学研究委员会(MRC)量表、评估残疾的炎症性Rasch综合残疾量表(I-RODS)]得分进行分析,观察他克莫司治疗CIDP的疗效。结果 两因素重复测量设计的方差分析显示,在时间因素和组别因素无交互作用的情况下(P>0.05),时间因素对两量表的主效应差异均有统计学意义(P<0.05),且两量表得分均随时间呈上升趋势(P<0.05)。2组患者在开始维持治疗后第3、6、12个月两量表得分差异均无统计学意义(P>0.05)。为期1 a的观察试验结束时,2组患者改善率及复发率差异均无统计学意义(P>0.05)。结论 无论他克莫司还是糖皮质激素作为CIDP的维持治疗均能使患者获益,且他克莫司用于CIDP维持治疗的疗效不劣于糖皮质激素。

褪黑素对创伤后应激障碍大鼠下丘脑-垂体-肾上腺轴的影响

目的:探讨褪黑素(MLT)对足部电击所致创伤后应激障碍(PTSD)大鼠下丘脑-垂体-肾上腺(HPA)轴的影响。方法:利用足底电击法制备大鼠PTSD模型,通过腹腔注射方法给予治疗组大鼠MLT。通过拒俘反应测试检测大鼠的行为学变化,利用real time RT-PCR方法检测下丘脑中促肾上腺皮质激素释放激素(CRH)mRNA的表达,利用酶联免疫吸附试验(ELISA)检测血清中促肾上腺皮质激素(ACTH)、肾上腺素(EPI)和糖皮质激素(GC)的含量。结果:PTSD组大鼠拒俘反应明显(P<0.05),下丘脑中CRH mRNA表达升高(P<0.05),血清中ACTH和EPI明显升高(P<0.05),但是GC水平下降(P<0.05)。MLT治疗后可以明显缓解PTSD大鼠拒俘反应(P<0.05),同时降低下丘脑中CRH mRNA表达(P<0.05),降低血清中ACTH和EPI水平并升高GC的水平(P<0.05)。结论:MLT治疗可缓解PTSD大鼠的症状,并恢复HPA轴的神经内分泌平衡。