Human monoeyte leukemia cell line THP-1 was stimulated with lipopolysaccharide （LPS） to simulate the sepsis model and the expression of human glucocorticoid receptor-α （GR-α） mRNA in montocytes with endotoxin to...Human monoeyte leukemia cell line THP-1 was stimulated with lipopolysaccharide （LPS） to simulate the sepsis model and the expression of human glucocorticoid receptor-α （GR-α） mRNA in montocytes with endotoxin tolerance was investigated. THP-1 cells were cultured in serum-free medium, randomly divided into groups A, B, C, D and E, and stimulated with 0, 10, 10, 100, 0 ng/mL LPS for 24 h followed with 100, 100, 10, 100, 0 ng/mL LPS for another 24 h respectively. The expression of GR-α mRNA was detected by semi-quantitative reverse transcriptional polymerase chain reaction. Tumor necrosis factor-α （TNF-α） was determined by enzyme linked immunosorbent assay （ELISA）. The results showed that the A values of GR-α/β-actin in groups A, B, C, D and E was 0. 607±0. 006, 0. 368±0. 005, 0. 484±0. 008, 0. 509±0. 004 and 0. 564±0. 014 respectively with the difference being significant among the groups （P〈0. 05）. The GR-α mRNA expression was negatively correlated with the TNF-α expression （P〈0. 01）. It was concluded that the down-regulation of the expression of GR-α mRNA in endotoxin tolerance THP-1 cells might play an important role in the development of endotoxin tolerance in THP-1 cells.展开更多
文摘Human monoeyte leukemia cell line THP-1 was stimulated with lipopolysaccharide （LPS） to simulate the sepsis model and the expression of human glucocorticoid receptor-α （GR-α） mRNA in montocytes with endotoxin tolerance was investigated. THP-1 cells were cultured in serum-free medium, randomly divided into groups A, B, C, D and E, and stimulated with 0, 10, 10, 100, 0 ng/mL LPS for 24 h followed with 100, 100, 10, 100, 0 ng/mL LPS for another 24 h respectively. The expression of GR-α mRNA was detected by semi-quantitative reverse transcriptional polymerase chain reaction. Tumor necrosis factor-α （TNF-α） was determined by enzyme linked immunosorbent assay （ELISA）. The results showed that the A values of GR-α/β-actin in groups A, B, C, D and E was 0. 607±0. 006, 0. 368±0. 005, 0. 484±0. 008, 0. 509±0. 004 and 0. 564±0. 014 respectively with the difference being significant among the groups （P〈0. 05）. The GR-α mRNA expression was negatively correlated with the TNF-α expression （P〈0. 01）. It was concluded that the down-regulation of the expression of GR-α mRNA in endotoxin tolerance THP-1 cells might play an important role in the development of endotoxin tolerance in THP-1 cells.
