A simple method for rapid estimation of the enantioselectivity of lipase in resolution of chiral esters is described. The enantioselectivity of lipase can be estimated rapidly through comparing the difference of hydro...A simple method for rapid estimation of the enantioselectivity of lipase in resolution of chiral esters is described. The enantioselectivity of lipase can be estimated rapidly through comparing the difference of hydrolysis rates for the racemic ester and its slow reacting enantiomer under the same condition because the difference mainly depends on the enantioselective ratio（E values）. The higher the enantioselectivity of enzyme, the larger the difference of hydrolysis rate. The bromothymol blue（BTB） can be used as pH indicator for microplate reader to monitor the formation of acid in lipase-catalyzed hydrolysis of esters. This method has been successfully used to rapidly estimate the enantioselectivity of several lipases in the resolution of glycidyl butyrate.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.20072011, 20272017)the Foundation of Research Program of Jilin University, China.
文摘A simple method for rapid estimation of the enantioselectivity of lipase in resolution of chiral esters is described. The enantioselectivity of lipase can be estimated rapidly through comparing the difference of hydrolysis rates for the racemic ester and its slow reacting enantiomer under the same condition because the difference mainly depends on the enantioselective ratio（E values）. The higher the enantioselectivity of enzyme, the larger the difference of hydrolysis rate. The bromothymol blue（BTB） can be used as pH indicator for microplate reader to monitor the formation of acid in lipase-catalyzed hydrolysis of esters. This method has been successfully used to rapidly estimate the enantioselectivity of several lipases in the resolution of glycidyl butyrate.
