Syntheses,Structures and Luminescent Property of Two Supramolecular Complexes Containing N-P-Acetamidobenzenesulfonyl-glycine Acid Ligand

Making use of N-P-acetamidobenzenesulfonyl-glycine acid （abbreviated as abglyH2）,two transitional metal complexes [Zn（abglyH）（phen）2]ClO4·H2O （1） and Co（abglyH）2（bipy）2（H2O）2 （2） （phen = 1,10-phenanthroline,bipy = 4,4＇-bipyridine） have been synthesized under mild conditions and characterized by IR,elemental analysis and X-ray diffraction analysis. Complex 1 is a monomeric compound which is further assembled by intermolecular hydrogen bonds and π-π interaction into a 2-D supramolecular network. Complex 2 is also a monomeric compound and further connected by intermolecular hydrogen bonds to form a 2-D supramolecular network. Fluorescent analysis shows that complex 1 has an emissive maximum at 364 nm in the solution state at room temperature.

Synthesis and Crystal Structure of Glycin·3,5-Dihydroxybenzoic Acid

The title compound glycin.3,5-dihydroxybenzoic acid （GDB） has been synthesized and characterized by single-crystal X-ray analysis. It crystallizes in orthorhombic, space group Pca21 with a = 25.513（10）, b = 4.668（2）, c = 9.150（4） A°, Z= 4, V= 1089.8（8） A°^3, C9H13NO7, Mr= 247.20, Dc = 1.507 g/m^3,/z（MoKa） = 0.132 mm^-1, F（000） = 520, R1 = 0.0322 and wR2 = 0.0611 for 885 observed reflections （I 〉 2σ（I））. The title compound is a 1：1 molecular complex of glycin and 3,5-dihydroxybenzoic acid, which is linked by O-H…O and N-H…O hydrogen bonds to form a three-dimensional framework. Test of SHG （second harmonic generation） indicates that NLO generation of the crystal is higher than that of KDP.

Syntheses,Structures and Luminescent Properties of Two Zinc Complexes Containing N-P-Acetamidobenzenesulfonyl-glycine Acid Ligand

Using N-P-acetamidobenzenesulfonyl-glycine acid （abbreviated as abglyH2） as a ligand, two zinc（II） complexes [Zn（abglyH）2（bipy）2（H2O）2], （1） and {[Znz（abgly）2（bipy）2（H2O）2]. 2（H2O）}n （2） （bipy = 4,4＇-bipyridine） have been synthesized under mild conditions and characterized by IR, elemental analysis and X-ray diffraction analysis. Complex 1 is a monomeric compound, which is further assembled by intermolecular hydrogen bonds and π-π interactions into a 3-D supramolecular network. Complex 2 adopts a one-dimensional double chain structure and is further linked by hydrogen bonds to form a 2-D structure. Fluorescent analysis shows that complex 1 has an emissive maximum at 337 nm and complex 2 exhibits an emissive maximum at 339 nm in the solution state at room temperature.

Organophosphonotungstic HPA of Keggin Type with Sulfo,Taurine and Glycine Substituted Ethylphosphonic Acids as the Coordinate Center

The title compounds 2ac were synthesized and characterized for the first time. Their proton numbers are around 3.2±0.15 and are not in proportion with the valid acidity-basicity of the central phosphonic acids. The entirety of phosphonic acids 1a-c is as the coordinate center of heteropoly anions of 2ac.

Syntheses and Structures of Three Cobalt Metal-organic Frameworks with 3-(Sulfonyl-glycine)benzoic Acid

Three new coordination networks based on a versatile and unsymmetric building block 3-（sulfonyl-glycine）benzoic acid （HAL） ligand and inorganic Co（Ⅱ） salt, [Co（HL）（bipy）2]. ClO4·1.5H2O （1）, [Co3（L）2（H2O）6]·4H2O （2） and [Co（HL）（bpp）（H2O）2]·2H2O （3） （bipy = 2,2-bipyridine, bpp = 1,3-bi（4-pyridyl）propane）, have been synthesized in aqueous media and structurally characterized by single-crystal X-ray diffraction. Reaction of Co salt with H3L afforded a neutral 1D alternating chain, which shows a 3-D supramolecular network through hydrogen bonds. When the auxiliary 2,2-bipyridine is used, a mononuclear unit is constructed; if using auxiliary ligand bpp, a neutral 1-D uniform chain is obtained and further extended into a 3-D supramolecular structure through versatile hydrogen bonds. The H3L ligand in 1 adopts a bidentate chelating coordination mode while that in 2 and 3 assumes a pentadentate bridging and a bis-monodentate bridging modes, respectively. This work and our previous work evidently demonstrate that the structures vary with altering the second ligands.

外源茉莉酸对大豆中异戊烯黄酮Glyceollins及其前体Glycinol累积的影响

以中黄13号大豆种子为材料,取生长8天的健康子叶,在其表面挖出约2×2mm的伤口,将50μl不同浓度的茉莉酸溶液和1mM的硝酸银溶液滴在伤口处,黑暗中(25℃)保温不同时间后取样,经甲醇提取,用高压液相色谱(HPLC)测定其中异戊烯化异黄酮glyceollins及其前体glycinol 的累积情况.结果显示,健康子叶中没有glyceollins和glycinol的累积,1mM硝酸银比茉莉酸具有更强的诱导glyceollins和glycinol累积的能力,glyceollins的累积与茉莉酸浓度呈负相关.在各种处理中,glyceollinⅠ、Ⅱ、Ⅲ所占比例分别为:50%～80%、10%～40%和10%～20%,但是glyceollin三种异构体的比例随保温时间的延长而有所变化,glyceollin Ⅰ比例逐渐下降,glyceollin Ⅱ所占比例逐渐升高,在1mM硝酸银处理的子叶中表现更为明显.

How Many Methanol Molecules Can Solvate a Glycine: a PBE1PBE Approach

Microsolvation of glycine in methanol clusters is explored by the use of DFT calculation method. The lowest energy conformations within 16.72 kJ·mol^-1 of the glycine clustering with one to six methanol molecules, which are obtained at the B3LYP/6-31＋G（d） level of theory, are reoptimized at PBE1PBE/6-311＋G（d,p）. The calculated results agree with our previous results with B3LYP （Chin. J. Chem. Phys. 22 （2009） 577） that the clusters of two forms （Z- and N-form） tend to be isoenergetic when the number of the solvate molecules reaches six. Furthermore, this result is in good agreement with the experiment of the tryptophan-methanol clusters, implying that the present treatments are reasonable and reliable. The results also indicate that nine methanol molecules are not enough to fully solvate a glycine molecule, and a tentative estimation is obtained that ten methanol molecules may fully solvate a glycine molecule, which consists with the experiment results.

Characterization and Biodegradation Studies for Interpenetrating Polymeric Network (IPN) of Chitosan-Amino Acid Beads

The paper describes the synthesis of pH sensitive interpenetrating polymeric network (IPN) beads composed of chi-tosan, glycine, glutamic acid, cross linked with glutaraldehyde and their use for controlled drug release. The drug was loaded into beads by varying their composition such as, amount of crosslinker glutaraldehyde, ratio of chitosan, glycine and glutamic acid. The beads were characterized by fourier transform infrared (FTIR) spectroscopy to confirm the cross linking reaction and drug interaction with crosslinked polymer in beads, Scanning Electron Microscopy (SEM) to understand the surface morphology and Differential scanning calorimetry (DSC) to find out the thermal stability of beads. X-Ray Diffraction (XRD) investigation was carried out to determine the crystalline nature of drug after loading into chitosan-glycine-glutamic acid IPN beads. Results indicated amorphous dispersion of chlorpheniramine maleate (CPM) in the polymeric matrix. The swelling behavior of the beads at different time intervals was monitored in solutions of pH 2.0 and pH 7.4. The release experiments were performed in solutions of pH 2.0 and pH 7.4 at 37oC using chlorpheniramine maleate (CPM) as a model drug. The swelling behavior and release of drug were observed to be dependent on pH, degree of cross linking and their composition. The results indicate that the cross linked IPN beads of chitosan-glycine-glutamic acid might be useful as a vehicle for controlled release of drug. The kinetics of drug release from beads was best fitted by Higuchi’s model in which release rate is largely governed by rate of diffusion through the matrix.

Analysis of amino acid constituents of gallstones

IM To seek effective drugs to dissolve bilirubin, glycoprotein and black stones and better lithotriptics for cholesterol stones, and study the amino acid constituents of gallstones.METHODS According to the characteristics of infrared spectrum and the contents of bilirubin tested by semiquantitative chemical analysis, 30 of 148 cases of gallstones were selected and divided into 5 groups. Amino acids of the 30 cases were detected by high speed chromatography.RESULTS The quantity of amino acids in black stones was the highest (2269mg/g) and that of pure cholesterol stones was the lowest, only 14mg/g in a case. In five groups of gallstones, the contents of amino acids were: black stone > mixed bilirubin stone and glucoprotein stone > mixed cholesterol stone > pure cholesterol stone. The proportions were: 9595∶2902 and 2805∶578∶1. Aliphatic amino acids accounted for about 50% of the total amino acids in gallstones and glycine 153% of the total amount of 17 kinds of amino acids.CONCLUSION In mixed stones, the higher level of bilirubin, the higher contents of amino acids. The acidic amino acids of bilirubin stones were relatively higher than those of cholesterol stones.

Equilibria and Stability in Glycine, Tartrate and Tryptophan Complexes, Investigation on Interactions in Cu(II) Binary and Ternary Systems in Aqueous Solution

The acidity and stability constants of M(Gly)1, M(Ttr)1, and M(Trp)1 M: Cu2+, Cu(Bpy2)2+, and Cu(Phen3)2+ complexes, were determined by potentiometric pH titration. It is shown that the stability of the binary Cu(L), (L: Gly, Ttr, and Trp) complex is determined by the basicity of the carboxylate group on one side and amino group on the other side. It is demonstrated that the equilibrium, Cu(Ha4)2+ + Cu(L) Cu(Har)(L) + Cu2+, is displacement due to the well known experience that mixed ligand complexes formed by a divalent 3d ion, a heteroaromatic N base and an O donor ligand possess increased stability. The stability constants of the 1:1 complexes formed between Cu2+, Cu(Bpy)2+ or Cu(Phen)2+ and L2-, were determined by potentiometric pH titration in aqueous solution (I = 0.1 M, NaNO3, 25?C). The order of the stability constants was reported. A comparative investigation between ternary complexes of Trp, Ttr, and Gly is made. The comparison of stability constants of these ternary complexes show that Cu(Har)(Trp) is found near 100% in closed form but Cu(Har)(Gly) exists in open form. The differences between the above mentioned stability constants are based on stacked form of Cu(Har)(Trp). The last provides increased stability.

Effect of single-use versus combined-use moschus and diazepam on expression of amino acid neurotransmitters in the rat corpus striatum

The present study analyzed expressional changes of excitatory neurotransmitters and inhibitory neurotransmitters in the rat corpus striatum after single-use and combined-use diazepam and Chinese herb moschus. The influence of moschus on the central nervous system was analyzed, in particular whether moschus increased penetration of other drugs into the brain. Reverse-phase high-performance liquid chromatography, which included pre-column derivation with orthophthaladehyde detection, showed varied increased levels of excitatory neurotransmitters, including aspartate and glutamate, and inhibitory neurotransmitters, including glycine and Y-aminobutyric acid, in the corpus striatum after treatment with moschus alone, diazepam alone, or a combination of both. Compared with the diazepam group, aspartate levels significantly decreased at 30 and 60-105 minutes after combined treatment with moschus, while glutamate significantly increased at 45 and 75-105 minutes, glycine levels significantly increased at 105 minutes, and γ-aminobutyric acid increased at 30 and 75-105 minutes. These findings suggested that moschus increased the inhibition effects of diazepam on the brain.

Simultaneous Determination of Four Amino Acids in Fufang Ejiao Buxue Granule by HPLC

[Objectives]To establish an High Performance Liquid Chromatography(HPLC)method for simultaneous determination of four amino acids in Fufang Ejiao Buxue Granule.[Methods]The quantitative analysis was carried out with a Waters Sunfire C 18 column(4.6 mm×250 mm,5μm).A binary mobile solvent was used:mobile solvent A was acetonitrile-0.1 mol/L sodium acetate solution(adjusting pH to 6.5 with 36%acetic acid)(7∶93)and mobile solvent B was acetonitrile-H 2O(4∶1).The mobile phase was delivered at a flow rate of 1.0 mL/min with a gradient elution profile(0-13 min,100%A→93%A;13-17.9 min,93%A→88%A;17.9-29.0 min,88%A→85%A;29-39 min,85%A→66%A;39-45 min,66%A→0%A).The column temperature was at 43℃.The detection wavelength was 254 nm.[Results]The injection volume of L-hydroxyproline,glycine,alanine,and L-proline showed a good linear relationship with the chromatographic peak area in the range of 0.012 to 0.117,0.022 to 0.218,0.010 to 0.097,0.016 to 0.160μg,separately.The average recovery rate(n=6)was 96.4%,97.3%,97.1%,and 99.4%,respectively;the relative standard deviations were 1.2%,1.9%,1.7%,and 0.9%,respectively.[Conclusions]This method is simple in operation and good in reproducibility,and provides a reliable method for controlling the quality of Fufang Ejiao Buxue Granules.

Effect of GmLEC1-A Expression on ABA Content at Germination Stage in Soybean(Glycine max)

LEAFY COTYLEDON1(LEC1) is a key regulator of seed maturation, which gives embryos the ability to withstand desiccation. In this study, a novel transcription factor that is homologous to LEC1 in soybean(Glycine max) was isolated from Dongnong 50 by homologous cloning and was named as GmLEC1-A(GenBank accession number: MF681785). Sequence analysis showed that GmLEC1-A contained conserved B regions, which were functional domains of H4 factor. The relative expression level of GmLEC1-A was the highest in seeds of the soybean cultivar Dongnong 50. To verify the function of GmLEC1-A, ectopic expression of Arabidopsis thaliana and over-expression of soybean plants were generated. In Arabidopsis, the expression of GmLEC1-A restored the tolerance of lec1 mutant to seed drying, indicating that GmLEC1-A was a functional homolog of LEC1,and it might regulate the maturation phase of seed development. In soybean, over-expression of GmLEC1-A caused slower growth and lower germination rates as compared to that of wild-type soybeans. Furthermore, over-expression of GmLEC1-A seemed to increase the level of endogenous abscisic acid(ABA) at the germination stage. These results suggested that GmLEC1-A had a function in regulating ABA content at the germination stage.

Isolation and Characterization of Storage Protein Subunit-null-dwarf Mutants in Soybean(Glycine max(L.) Merr.)

Dwarfing is useful to reduce plant height,when breeding high-yielding and non-lodging crops.In this study,a set of natural storage protein subunit-null dwarf mutants of soybean was reported that showed strongly reduced plant stature and deficiency in various 7S and 11S subunits,designated as snd1 mutants.Under normal growth conditions,the snd1 mutants showed a severe dwarf phenotype,with plant height of about 25 cm.Compared with wild-type DN47,the mutant snd1 exhibited no obvious morphological differences at the early stage of development.All the snd1 mutants examined had fewer nodes and shorter than normal internodes;the leaves were similar in shape to normal parents,but were dark-green at the mature stage.The flower size was similar to DN47;however,the flowering period was shorter than in the wild-type.Significant variation was noted for protein content,oil content of the seeds and size of seeds(weight of 100 seeds)among 17 snd1 dwarf lines.Genetic analysis indicated that the dwarfism of snd1 was controlled by a single recessive gene.The snd1 dwarf mutant had markedly different dynamic levels of the endogenous hormones gibberellin(GA),brassinosteroid,indole-3-acetic acid and abscisic acid,at the seedling stage.Exogenous GA3 treatment led to recovery of the plant height phenotype of the snd1 mutant;GA3 at 0.1 mm had the largest effect on enhancing plant height.Using molecular markers,snd1 gene was approximately mapped in an interval of 603 kb between markers Satt166 and Satt561 on chromosome 19.Snd1 mutant provided valuable material for hypoallergenic soybean breeding and the snd1 gene might be a novel gene related to plant height in soybean.

^(99)mTc-c(RGD)_(2) SPECT/CT与^(18)F-FDG PET/CT 显像对肝泡型包虫病浸润带新生血管表达的相关性

目的探讨^(99)mTc肼基烟酰胺聚乙二醇双环RGD肽[^(99)mTc-c(RGD)2]SPECT/CT与^(18)氟-氟代脱氧葡萄糖(^(18)F-FDG)PET/CT显像对肝泡型包虫病(HAE)增殖浸润带新生血管表达的相关性。方法采用前瞻性研究方法,收集2023年1月~2024年1月在新疆医科大学第一附属医院收治的34例HAE患者资料,所有患者均行全身18F-FDGPET/CT、腹部^(99)mTc-c(RGD)2 SPECT/CT显像及手术治疗,对34例患者的41个HAE病灶组织行常规HE染色及CD34免疫组织化学染色,检测边缘带微血管密度(MVD)计数。分析HAE浸润带18F-FDGPET/CT显像的最大标准摄取值(SUVmax)及摄取比值(TBR)、^(99)mTc-c(RGD)2 SPECT/CT显像靶非靶比值(T/NT)分别与MVD计数的相关性;比较HAE浸润带^(99)mTc-c(RGD)2 SPECT/CT显像阳性(T/NT>1)及阴性(T/NT<1)两组间MVD计数的差异。结果41个HAE病灶浸润带^(99)mTc-c(RGD)2 SPECT/CT显像T/NT值与MVD计数两组间呈高度正相关性(r=0.823,P<0.01);^(18)F-FDGPET/CT显像SUVmax值、TBR与MVD计数均无相关性(P>0.05)。HAE浸润带^(99)mTc-c(RGD)2 SPECT/CT显像阳性组17个与阴性组24个病灶MVD计数分别为89.00±9.87、43.08±18.36个/高倍视野,组间差异有统计学意义(P<0.05)。结论HAE^(99)mTc-c(RGD)2 SPECT/CT显像RGD的摄取程度与HAE浸润带新生血管表达呈高度正相关,^(99)mTc-c(RGD)2 SPECT/CT显像可间接反应HAE的侵袭性,可作为评价病灶侵袭性经济、无创的检查手段之一,并且可与18F-FDGPET/CT联合从HAE浸润带的新生血管表达及炎性活性两方面共同评价其生物学活性。

RGD多肽水凝胶对Tenon囊成纤维细胞活化和YAP信号的影响

目的 探究不同浓度的精氨酸-甘氨酸-天冬氨酸(RGD)多肽水凝胶,对Tenon囊成纤维细胞(HTF)活化和Yes相关蛋白(YAP)表达的影响。方法 制备0.5%、1.0%、2.0%三种浓度RGD多肽水凝胶,透射电子显微镜观察其内部微观结构,流变学检测弹性模量(E)。构建SD雄性大鼠结膜损伤模型后,分为空白组(不作处理)、低浓度RGD组(结膜下注射0.5%RGD多肽水凝胶)、中浓度RGD组(结膜下注射1.0%RGD多肽水凝胶)、高浓度RGD组(结膜下注射2.0%RGD多肽水凝胶),观察1周后取材,Masson染色观察大鼠结膜处胶原纤维增生情况,免疫组织化学检测大鼠结膜YAP、平滑肌肌动蛋白-α(α-SMA)表达情况。利用转化生长因子-β2(TGF-β2)刺激HTF活化构建瘢痕化细胞模型,按照实验要求分为对照组、 0.5%RGD水凝胶组、1.0%RGD水凝胶组、2.0%RGD水凝胶组、正常组,培养24 h后,Western blot检测HTF中YAP、结缔组织生长因子(CTGF)、α-SMA、纤连蛋白(FN)和I型胶原蛋白(Col I)的蛋白相对表达量;激光共聚焦显微镜观察细胞骨架蛋白(F-actin)和YAP蛋白表达定位。结果 电镜下观察可见,RGD多肽水凝胶内部纳米纤维交联密度随多肽浓度增加而升高,流变学测得0.5%、1.0%及2.0%RGD多肽水凝胶弹性模量(E)依次约为0.067 kPa、0.150 kPa、2.170 kPa。Masson染色结果显示,除中浓度RGD组大鼠结膜胶原纤维增生面积比明显低于空白组外(P<0.05),低浓度组、高浓度组与空白组差异均无统计学意义(均为P>0.05)。免疫组织化学结果显示,与空白组相比,低浓度RGD组、中浓度RGD组、高浓度RGD组大鼠YAP及α-SMA蛋白相对表达量均下降,中浓度RGD组下降最明显,差异均有统计学意义(均为P<0.05)。细胞免疫荧光染色结果显示,与对照组相比,0.5%RGD水凝胶组、1.0%RGD水凝胶组、2.0%RGD水凝胶组HTF的F-actin绿色荧光减弱,YAP红色荧光从细胞核转位于细胞质表达。Western blot检测结果显示,与对照组相比,0.5%RGD水凝胶组、1.0%RGD水凝胶组、2.0%RGD水凝胶组HTF中YAP、CTGF、α-SMA、FN及Col I蛋白相对表达量均下降,1.0%RGD水凝胶组下降最明显,差异均有统计学意义(均为P<0.05)。结论 RGD多肽水凝胶的最佳作用浓度为1.0%,其通过抑制YAP蛋白的表达水平及其核转位,减少HTF的活化及其纤维化蛋白的表达,从而产生抗瘢痕效果。

叶施生物刺激剂与减量灌溉对芹菜生长、产量及灌溉水利用效率的影响

生物刺激剂在增强农作物非生物逆境耐性方面的潜在价值备受各方关注。采用完全随机区组设计,考察了生物刺激剂(清水对照组CK;黄腐酸FA;甜菜碱GB)叶施与减量灌溉(常规灌溉CI;73%常规灌溉RI)措施相耦合对设施芹菜生产的影响,旨在探索生物刺激剂的节水增产增效潜力。分析了芹菜光合作用、生物量、产量、灌溉水利用效率、营养和安全品质以及矿质营养元素氮磷钾吸收量等指标。结果表明,常规及减量灌溉条件下,叶施FA和GB显著降低了芹菜的叶片温度,大幅提升了芹菜的叶片蒸腾效率;叶喷2种生物刺激剂还明显提升了芹菜地上部氮磷钾元素吸收量和单棵重、产量、净收入、灌溉水利用效率,叶施FA处理芹菜的生长及产量明显优于GB。品质调控方面,GB优于FA。与CI相比,RI处理未引起芹菜减产,大幅提升了芹菜灌溉水利用效率。RIFA和RIGB组合处理的芹菜产量、水分利用效率和净收入均明显大于CICK,表明2种生物刺激剂在农业增产增效及节水领域均具有应用价值。

天冬氨酸和谷氨酸及其甘氨酸二肽调控Caco-2细胞钙离子吸收的研究

为了探究天冬氨酸和谷氨酸及其甘氨酸二肽对钙离子吸收的影响机理,本文利用等温滴定量热技术、电化学、Caco-2细胞模型结合量子化学计算(密度泛函理论)对天冬氨酸和谷氨酸及其甘氨酸二肽与钙离子的相互作用进行了探究。结果表明,天冬氨酸和谷氨酸通过焓和熵驱动的放热反应与钙离子形成复合物,而其形成的二肽则是通过熵驱动的吸热反应与钙离子结合。此外,二肽比单独的氨基酸表现出更强的钙离子结合能力。量子化学计算表明,氨基酸/二肽中的羧基为钙离子的主要结合位点,在碱性条件下,会导致钙离子结合位点转移,氨基也可以参与钙离子结合。Caco-2细胞吸收实验表明,天冬氨酸和谷氨酸及其二肽均可促进钙的吸收,其中甘氨酸-谷氨酸二肽(Gly-Glu)的促进效果最好,促钙吸收率为1.33±0.115。此外,促钙吸收能力与钙离子结合能力之间没有明确的相关性,与其电子亲和力的关系更为密切。研究结果解释了天冬氨酸、谷氨酸及其甘氨酸二肽与钙之间的相互作用,为进一步开发钙补充剂提供科学依据。

注射用透明质酸钠复合溶液中氨基酸含量测定

目的 建立氨基酸分析仪测定注射用透明质酸钠复合溶液中脯氨酸、甘氨酸和丙氨酸含量的方法。方法供试品中的氨基酸经阳离子交换树脂柱分离,于135℃下与茚三酮进行柱后衍生化显色反应,脯氨酸的检测波长为440 nm,其他氨基酸的检测波长为570 nm,外标法计算各氨基酸含量。结果 脯氨酸、甘氨酸和丙氨酸线性范围分别为0.0148~0.074,0.0207~0.103,0.0247~0.1235 mg/ml(r^(2)=0.9998);平均回收率分别为98.2%,98.5%和98.3%,RSD分别为0.55%,0.22%和1.40%;定量限分别为13.28,0.828,0.988μg。结论 方法重现性好、灵敏度高、检测结果准确且操作简单,适用于注射用透明质酸钠复合溶液中氨基酸含量的测定。

天然冰片对小鼠脑内氨基酸类神经递质含量的影响

目的研究小鼠口服天然冰片后脑内冰片含量的变化以及对氨基酸类神经递质含量的影响。方法小鼠灌胃天然冰片,于给药前及给药后0.083,0.167,0.25,0.333,0.5,0.75,1,1.5,2,2.5,3,4 h分别取脑,采用GC-MS法测定冰片含量,HPLC-FLD法测定氨基酸类神经递质含量。结果小鼠口服天然冰片1.2 g/kg后5 min开始出现兴奋症状,持续10~15 min转为抑制状态。天然冰片给药后5 min即可在脑内测得,60 min达到峰值,消除半衰期1.66 h,AUC为326.7μg.h.g-1。脑内天门冬氨酸从给药后0.083 h至1 h含量显著增加,谷氨酸在给药后0.333 h含量显著增加,在给药后1.5 h至4 h含量显著降低,γ-氨基丁酸从给药后0.167 h至4 h含量均显著增加,甘氨酸含量无明显变化。结论天然冰片对中枢神经系统具有兴奋/抑制的双向作用,其作用机制与其对中枢兴奋/抑制性氨基酸类神经递质释放的调控作用有关。