Influence of late emerging weeds in glyphosate-resistant corn

Fifteen field trials were conducted from 2009 to 2011 in Ontario, Canada and Michigan, USA to determine how long glyphosate-resistant corn needs to be kept weed-free after emergence to prevent yield loss. Data were separated into two environments based on when yield loss first occurred after glyphosate application. In Environment 1 (4/15 sites) yield was not reduced when corn was kept weed-free until the 4-leaf stage. However, in Environment 2 (11/15 sites) there was no yield loss when corn was kept weed-free up to the 2-leaf stage. The most prominent weeds were velvetleaf, redroot pigweed, common ragweed, common lambsquarters and foxtail species. While later emerging weeds did not necessarily impact corn yield, weeds emerging after the 2- and 4-leaf corn stage likely produced seed that was added to the soil seed bank. Weeds emerging after 6-, 8-, and 10-leaf corn growth stages were small (low biomass/seedlings) and most likely did not reach reproductive maturity. Based on this research, corn must be maintained weed-free up to the 4-leaf stage. Any weeds emerging after that did not influence corn yield.

Fungal Flora on Weeds in the Cashew (Anacardium occidentale L.) Orchard in Côte d’Ivoire

Since 2015, Côte d’Ivoire has been the world’s largest cashew producer. However, cashew orchards in Côte d’Ivoire are infected by fungal diseases that weaken production. And the contribution of weeds to the spread of these diseases is not yet understood. This study was initiated with the aim of establishing the role of weeds in the proliferation of pathogenic fungi in orchards. It consisted of a survey of weeds showing disease symptoms in cashew orchards in Côte d’Ivoire from February 2021 to July 2022. The itinerant method was used for the weed inventory. Symptomatic leaves were collected and sent to the laboratory for diagnosis on PDA (Potatoes Dextrose Agar) medium. In total, 50 species in 46 genera and 23 families were recorded. Laboratory diagnosis of the samples showed that 80% of the weeds identified harboured pathogenic fungi. The highest infection rates were obtained on Danielia oliveri R. (99.33% to 100%), Vitellaria paradoxa G. (100%), Pterocarpus erinaceus P. (83.91% to 99.33%), Micuna pruriens L. (98.33% to 100%) and Isoberlinia doka C. et S. (56.33% to 100%). The diagnosis revealed the presence of Lasiodiplodia sp, Colletotrichum sp, Pestalotia sp, Alternaria sp and Curvularia sp on weeds in the cashew orchard in Côte d’Ivoire.

Estimating Carbon Capture Potential of Fallow Weeds in Rice Cropping Systems

Weeds occurred during the fallow season can well perform the function of carbon(C)capture due to receiving little human disturbance.This study aimed to evaluate the C capture potential of fallow weeds in rice(Oryza sativa L.)cropping systems.A six-region,two-year on-farm investigation and a three-year tillage experiment were conducted to estimate C capture in fallow weeds in rice cropping systems.The on-farm investigation showed that the average mean C capture by fallow weeds across six regions and two years reached 112 g m^(-2).The tillage experiment indicated that no-tillage practices increased C capture by fallow weeds by 80%on average as compared with conventional tillage.The results of this study not only contribute to an understanding of C capture potential of fallow weeds in rice cropping systems,but also provide a reference for including fallow weeds in the estimation of vegetative C sink.

Two-Pass Weed Management with Preemergence and Postemergence Herbicides in Glyphosate-Resistant Soybean

There is little information on the efficacy and profitability of two-pass weed control strategies in soybean when a preemergence (PRE) residual herbicide is followed by glyphosate applied late postemergence (LPOST) under Ontario, Canada environmental conditions. Ten field trials were conducted during 2011-2013 in Ontario, Canada to determine the level of weed control, yield and net returns of various preemergence/postemergence programs in glyphosate-resistant soybean. Crop injury was 2% or less with the herbicides evaluated except for chlorimuron + flumioxazin (PRE) and pyroxasulfone + flumioxazin (PRE) which caused 4% and 7% visible injury in soybean, respectively. A single application of glyphosate resulted in variable weed control (73% - 98%) while the sequential application of glyphosate provided excellent weed control (98% - 100%). The control of all weeds 8 WAA after the LPOST glyphosate application was equivalent regardless of the PRE herbicide applied (96% - 100%). Soybean yield was equivalent to the weed free control regardless of the PRE herbicide applied. Soybean yield was lower than the sequential application of glyphosate with chlorimuron or pyroxasulfone/flumioxazin PRE fb glyphosate LPOST. Generally net return with the two-pass programs was equivalent to the sequential application of glyphosate. Net returns were lower than the sequential application of glyphosate with chlorimuron or s-metolachlor + flumetsulam followed by glyphosate LPOST. Based on these results, a sequential application of glyphosate or a two-pass program of a preemergence residual herbicide followed by glyphosate LPOST are the preferred weed management programs in glyphosate-resistant soybean. The two-pass programs have the potential to reduce selection pressure for glyphosate-resistant weeds.

Glyphosate-Resistant Giant Ragweed(Ambrosia trifida L.)in Ontario:Dose Response and Control with Postemergence Herbicides

Giant ragweed (Ambrosia trifida L.) is competitive with agronomic crops and can cause significant yield losses. Rapid adoption of glyphosate-resistant (GR) crops and a concomitant increase in the reliance on glyphosate for weed management has led to the evolution of GR giant ragweed in Ontario, Canada. Field studies were conducted to evaluate the level of resistance in giant ragweed biotypes from Ontario, and to evaluate the effectiveness of various postemer-gence (POST) herbicides in soybean (Glycine max L.). The effective dose (ED) to provide 50%, 80% and 95% giant ragweed control was up to 1658, 9991 and >43200 g?a.e.?ha–1 4 weeks after application (WAA), respectively. For effective control, growers would need to apply glyphosate 18 times greater than the recommended field application dose. Glyphosate applied at the recommended field dose of 900 g?a.e.?ha–1 provided up to 57% control and resulted in soybean yield equivalent to the weedy check. Cloransulam-methyl applied POST provided up to 99% control, reduced giant ragweed density 98%, reduced giant ragweed shoot dry weight 99% and resulted in soybean yield equivalent to the weedfree check. Chlorimuron-ethyl, fomesafen, imazethapyr and imazethapyr plus bentazon applied alone or with glyphosate did not provide adequate control of GR giant ragweed. Based on these results, some GR giant ragweed biotypes from Ontario have evolved a high level of resistance to glyphosate. Cloransulam-methyl applied POST was the only herbicide that provided adequate control and suggests that additional weed management tactics will need to be implemented in order to effectively manage GR giant ragweed.

Preplant and Postemergence Control of Glyphosate-Resistant Giant Ragweed in Corn

Glyphosate-resistant (GR) giant ragweed has recently been identified in southwestern Ontario and has the potential to be a significant problem for regional corn producers. Eight field trials [four with preplant (PP) and four with postemergence (POST) herbicides] were conducted from 2013 to 2014 on various Ontario farms infested with GR giant ragweed to determine the efficacy of PP and POST tank-mixes in corn. Glyphosate tank-mixed with atrazine, dicamba, dicamba/atrazine, mesotrione plus atrazine, flumetsulam, isoxaflutole plus atrazine, saflufenacil/dimethenamid-P, S-metolachlor/atrazine and rimsulfuron applied PP provided up to 54%, 95%, 93%, 95%, 40%, 89%, 91%, 50% and 93% control of GR giant ragweed and reduced dry weight 69%, 100%, 99%, 100%, 30%, 92%, 98%, 66% and 99%, respectively. POST application of glyphosate alone and tank-mixed with 2,4-D ester, atrazine, dicamba, dicamba/diflufenzopyr, dicamba/atrazine, bromoxynil plus atrazine, prosulfuron plus dicamba, mesotrione plus atrazine, topramezone plus atrazine, tembotrione/thiencarbazone-methyl and glufosinate provided up to 31%, 84%, 39%, 94%, 89%, 86%, 83%, 78%, 72%, 43%, 63% and 58% GR giant ragweed and reduced dry weight 55%, 99%, 72%, 99%, 99%, 98%, 96%, 96%, 93%, 89%, 91% and 95%, respectively. In general, PP control of GR giant ragweed was greater than POST applied herbicides evaluated. Based on these results, glyphosate tank-mixes containing dicamba or mesotrione plus atrazine applied PP, and dicamba applied POST will provide the most consistent control of GR giant ragweed in corn.

Enhanced Species Diversity Unlocked by Habitat Conservation in Agricultural Landscapes: New Perspectives on Ecological Weed Management and Sustainable Intensification—Species Diversity and Habitat Conservation

The impulse to remain profitable by increasing agricultural production levels in view of the greater demand for food, provided impetus to production intensification. The aim of this review is to summarise current literature, reporting specifically on the impact of production intensification on habitats and yield constraints caused by weeds. Secondly, in alleviating these effects over the short term, ecological measures that enhance species diversity in conserved habitats and promote semi-natural habitats in the agricultural landscape, are discussed. In large-scale intensive agriculture, weed control is predominantly rooted in agrochemical applications in the form of herbicides. Long lasting intensive agricultural practices show discord both with the promotion of the biodiversity of microbes belowground and aboveground and with organisms involved in the breaking down of plant material. The presence of native species in the surroundings, in combination with hedgerows and field margins, with a comparatively intricate and balanced variety of plants in a sheltered environment, are essential for settlement of benign insects, particularly in the face of intensive agricultural production. The promising tactic of advantageous seed predators enables decreased herbicide applications. Crop mosaics arranged to advance compatibility at the landscape scale are important to bolster pollination services and insect management, while ecological variety in the surroundings acts as a safety net for habitat diversity. Weed control in combination with different tactics of vegetation use, comprising cover cropping, hedgerows and field margins, sets up safe havens in the landscape, and improves the diffusion of complementary life forms. Field margins perform a meaningful natural function as point of provision for forage, safe havens and distribution passageways for pollinators and insect predators. Production practices that promote more heterogeneity and combine high density semi-natural safe havens and habitat conservation in agro ecosystems are beneficial to species diversity across trophic levels and contribute to agricultural production stability and food safety.

Influence of Herbicides on Competition Weed and Glyphosate-resistant Soybean

Competitions of light,nutrient and water between transgenic glyphosate resistant soybean and weed were evaluated with different herbicides(glyphosate,acetochlor,and the mixture of fomesafen and quizalofop-p-ethyl)in filed,which displayed that the application of the mixture of fomesafen and quizalofop-p-ethyl on soybeans had the best anti-herbicidal effect while glyphosate had the weakest effect.The half-life extended by herbicide mixture would affect the soil nutrients.Weed-soybean competitions affected the absorption of nutrients on soybean and yield.However,effective control of weeds could significantly reduce the weed density,improve the field through different layers of light transmittance and the absorption of nutrients on soybean,and enhance the comprehensive competitiveness of soybean.

Prospects and limitations of soil amendment and irrigation techniques for the water-saving public urban greenery and ephemeral weed management in the sandy soils of the United Arab Emirates

Public urban greenery greatly contributes to the residential and tourist value of cities in the Gulf Region,but due to the hyper-arid climatic conditions,the cost of irrigation and plant maintenance is very high.Existing strategies to reduce the monetary and ecological costs involve the cultivation of native xerophytic plantations,and/or the use of soil improvers to increase water-and nutrient-holding capacity of the sandy soils.Various soil improvers based on mineral,organic,or synthetic materials have entered the United Arab Emirates(UAE)market in recent years,but there is considerable uncertainty about how they should best be used in combination with ornamental plant stands involving xerophytic native plants.The present study investigated the effect of soil amendment and deep pipe irrigation on perennial ornamental plant stands involving native plants(Tephrosia appolinea(Gel.)Link in combination with Aerva javanica(Burm.f.)Juss.ex Schult.)and native-exotic plants(T.appolinea in combination with Ruelia simplex C.Wright)either or not topsoil and subsoil amendment with bentonite and hydrophobic sand under the irrigation water supply of less than 50%of reference evapotranspiration(ET0).After one year of cultivation,T.appolinea and A.javanica(native vs.native)produced high biomass and exhibited high water use efficiency(WUE)as compared with T.appolinea and R.simplex(native vs.exotic)combination given that no significant differences were found under the soil amendment treatments.All stands thrived under irrigation water supply far below what is usually supplied to exotic ornamental stands in public parks of the Al Ain City,the UAE.However,subsoil amendment in combination with deep pipe irrigation reduced the occurrence of weeds and increased the overall plant rooting depth.Our results suggest that subsoil amendment and irrigation up to 60-80 cm depth can potentially control ephemeral weed infestation,which is a great challenge in various plant production systems of the Gulf Region.The results of the present study suggest that the impact of soil amendment on the WUE of exotic plants is marginal and might not be economically justified.Replacing exotic with native ornamental plant species seems to have a far greater water-saving potential than the amendment of the soil,while weeds can be suppressed in the absence of topsoil moisture.

Artificial Intelligence for Maximizing Agricultural Input Use Efficiency: Exploring Nutrient, Water and Weed Management Strategies

Agriculture plays a crucial role in the economy,and there is an increasing global emphasis on automating agri-cultural processes.With the tremendous increase in population,the demand for food and employment has also increased significantly.Agricultural methods traditionally used to meet these requirements are no longer ade-quate,requiring solutions to issues such as excessive herbicide use and the use of chemical fertilizers.Integration of technologies such as the Internet of Things,wireless communication,machine learning,artificial intelligence(AI),and deep learning shows promise in addressing these challenges.However,there is a lack of comprehensive documentation on the application and potential of AI in improving agricultural input efficiency.To address this gap,a desk research approach was used by utilizing peer-reviewed electronic databases like PubMed,Scopus,Goo-gle Scholar,Web of Science,and Science Direct for relevant articles.Out of 327 initially identified articles,180 were deemed pertinent,focusing primarily on AI’s potential in enhancing yield through better management of nutrients,water,and weeds.Taking into account researchfindings worldwide,we found that AI technologies could assist farmers by providing recommendations on the optimal nutrients to enhance soil quality and deter-mine the best time for irrigation or herbicide application.The present status of AI-driven automation in agricul-ture holds significant promise for optimizing agricultural input utilization and reducing resource waste,particularly in the context of three pillars of crop management,i.e.,nutrient,irrigation,and weed management.

Linuron Biologically Effective Dose for Glyphosate-Resistant Giant Ragweed (<i>Ambrosia trifida</i>L.) Control in Soybean (<i>Glycine max</i>L.)

Glyphosate-resistant (GR) giant ragweed (Ambrosia trifida L.) was first identified in Canada in 2008 and has since been found throughout southwestern Ontario. Six field trials were conducted over a two-year period (2012, 2013) on Ontario farms with GR giant ragweed to evaluate the efficacy of linuron applied pre-plant (PP) in soybean (Glycine max (L.) Merr.). The dose required for 50%, 80%, and 95% GR giant ragweed control was 1238, 2959, and 6018 g·ai·ha-1 four weeks after application (WAA), respectively. The linuron dose needed for 50%, 80%, and 95% reduction in density was 1554, 3181, and 5643 g·ai·ha-1 and 1204, 2496, and 4452 g·ai·ha-1 for dry weight, respectively. Application of 7874 g·ai·ha-1 linuron was needed to obtain soybean yields that were 90% of the weed-free control;approximately 3.5 times the maximum field recommended dose. To achieve 95% and 98% yields, greater than 8640 g·ai·ha-1 linuron was required. Application of linuron plus glyphosate PP in soybean will help to control GR giant ragweed as well as reduce GR selection pressure.

Glyphosate-Resistant Common Ragweed Control in Corn with Postemergence Herbicides

Four field trials were conducted on a farm infested with glyphosate-resistant (GR) common ragweed during 2016 and 2017 to evaluate various postemergence (POST) herbicides for the control of GR common ragweed in GR corn. Dicamba at 600 g·a.i.·ha-1, dicamba/diflufenzopyr at 200 g·a.i.·ha-1, dicamba/atrazine at 1500 g·a.i.·ha-1, topramezone + atrazine at 12.5 + 500 g·a.i.·ha-1, bromoxynil + atrazine at 280 + 1500 g·a.i.·ha-1, glufosinate at 500 g·a.i.·ha-1 and 2,4-D ester at 560 g·a.i.·ha-1 provided 58% to 85% control at 4 WAA and 49% to 88% control at 8 WAA. Other herbicides evaluated controlled GR common ragweed 9% to 41%. Common ragweed density was reduced 97%, 95%, 95% and 87% and shoot dry weight was reduced 93%, 95%, 94% and 90% with bromoxynil + atrazine, dicamba, glufosinate and topramezone + atrazine applied POST in GR corn, respectively. Results show that dicamba, bromoxynil + atrazine, topramezone + atrazine and glufosinate applied POST are the most efficacious herbicides among the herbicides evaluated for the control of GR common ragweed in GR corn.

Investigation of Types and Hazard of Weeds in Coffea arabica Orchads in Nujiang River Basin

[Objective] Through the investigation of weeds in Coffea arabica orchads in NuJiang River basin, this study aimed to provide scientific guidance for the weed control and improving the ecological and economic benefits of the plantation of Cof- fea arabica. [Method] The types of weeds and the characteristics of weed occur- rence in Coffea arabica orchads in Nujiang River basin were investigated from July to August in 2012. [Result] The results showed that there were 69 types of weeds belonging to 21 families in Coffea arabica orchads. The predominant harmful types were shown as follows： Commelina nudiflora L. ＋ Leptochloa chinensis, Cyperus ro- tundus L. ＋ Bidens pilosa L. ＋ Eleusine indica, Imperata cylindrical ＋ Ageratum conyzoides L. ＋ Eupatorium odoratum L., Ageratum conyzoides L. ＋ Digitaria san- guinalis （L.） Scop. The preponderant weeds consisted mainly of 10 species, namely, Cyperus rotundus L., Commelina nudiflora L., Leptochloa chinensis, Digitaria san- guinalis （L.） Scop, Imperata cylindrical, Bidens pilosa L., Ageratum conyzoides L, E- upatorium odoratum L., Eleusine indica and Chenopodium serotinum L. [Conclusion] The result from this study is of great significance for the plantation of Coffea arabica in Nujiang River basin, as well as the control of weeds.

Chemical Control of Herbicide Monosulfuron Plus Propazine 44% WP against Weeds in Millet Fields and Study on Factors Influencing Yield of Millet

[Objective] The paper was to resolve the poor efficacy or phytotoxicity problems in promotion of herbicide monosulfuron plus propazine 44% WP. [Method] Using orthogonal test and Duncan＇s new multiple range statistical analysis with four factors four levels and three factors two levels, the effects of factors such as herbi- cide dose, irrigation water volume, quality of soil preparation and soil moisture content on chemical control of monosulfuron plus propazine 44% WP against weeds and millet yield were compared in the test. [Result] Seven factors all had great impact on control effect of monosulfuron plus propazine 44% WP against weeds, of which her- bicide dose, irrigation water volume, quality of soil preparation and soil moisture content were the most important factors affecting millet yield. According to millet yield and economic benefit, the optimal scheme to ensure safe, efficient and economic control of monosulfuron plus propazine 44% WP against weeds was as follows： the dose of monosulfuron plus propazine 44% WP was 1 800 g/hm2, the soil was treat- ed after sowing and before seedling emergence; the soil should be smooth and sol- id; the soil moisture should reach 15%; the fields should not be irrigated after herbi- cide application, and the damage of weeds in millet fields could be effectively con- trolled within 45 d during high efficacy duration, especially the damage of dicotyledonous weeds. [Conclusion] The study provided useful information for comprehensive technology of weeds control and ensuring high yield and good harvest of millet.

Effects of Rice-duck Mutualism on Diseases Insect Pests and Weeds and Economic Benefits

[Objective] Study on the effects of rice-duck mutualism on weeds and insects pests and economic benefits of paddy field. [Method] Comparison of the incidence of weeds, sheath blight and insects pest under rice-duck mutualism, conventional cultivation and control treatment, the yield and economic benefits were analyzed under the 3 treatments. [Result] Average occurrence of weeds in rice-duck mutualism group decreased by 2.33 and 52.0g ind/m^2 compared with that in conventional cultivation and control treatment ; the control rate of mutualism was up to 75% against rice hopper, but just between 25% -60% against rice leaf roller and Chilo suppressalis. The rates of diseased plant and diseased bell against rice sheath blight were higher and disease indices were lower compared with control group. The yield of mutualism group was identical with conventional cultivation, Which was greatly higher than that of control group. The results suggested an higher economic benefits and lower cost benefit ratio for rice-duck mutualism treatment. [Conclusion] Rice-duck mutualism gives birth to a positive effect to control the diseases, insect pests and weeds, as well as to economic benefits, providing basis on extension of rice-duck mutualism system.

Control Effects of 3 Kinds of Herbicides against Weeds in Dogbane (Apocynum venetum) Field

[ Objective] The paper was to explore the control effects of 3 kinds of herbicides against weeds in dogbane （Apocynum venetum） field. [ Method ] Using haloxyfop-R-mithyl and quizalofop-ethyl EC specifically killing monagenns gramineous weeds and chipton specifically killing dicotyledonous broad-leaf weeds, the control test against weeds in 2-year-old dogbane field was carried out. [ Result ] Haloxyfop-R-mithyl had good control effect against gramincous weeds, and its control effect against both barnyard weed （ Echinochloa crusgalli） and green bristleweed （ Setaria viridis ） was greater than 35.0% ; the control effects of quizalofop- ethyl EC against barnyard weed and green bristleweed were 14.8% and 28.6%, respectively; chipt0n had good control effect against Chenopodium album, and the control effect reached 30.8%. Moreover, 3 herbicides did not cause damage to dogbane. [ Conclusion] The study provide theoretical basis for completing cultivation technique of dogbane.

Chlorobenzenes in waterweeds from the Xijiang River (Guangdong section) of the Pearl River

The Xijiang River is the major source of water for about 4.5 millions of urban population and 28.7 millions of rural population. The water quality is very important for the health of the rural population. The concentration and distribution of chlorobenzenes （CBs） in both water and waterweeds collected from 4 stations in the Xijiang River （Gangdong section） of the Pearl River in April and November were determined. The result showed that nearly every congener of CBs was detected. The total contents of CBs （ZCBs） in the river water ranged from 111.1 to 360.0 ng/L in April and from 151.9 to 481.7 ng/L in November, respectively. The pollution level of CBs in the water in April was higher than that in November. The contents of ZCBs in waterweeds ranged from 13.53×10^2μg/g to 38.27×10^2μg/g dry weight （dw）. There was no significant difference between April and November in waterweeds. The distribution of CBs in roots, caulis, and leaves of Vallisneria spiralis L. showed different patterns. The leaves mainly contained low-molecular-weight CBs （DCBs）, whereas the roots accumulated more PCBs and HCBs. The average lgBCFlip （bioconcentration factor） of CBs ranged from 0.64 to 3.57 in the waterweeds. The spatial distribution character of CBs in the Xijiang River was： Fengkai County 〈 Yunan County 〈 Yun＇an County 〈 Gaoyao County according to the ZCBs, and the pollution deteriorated from the upstream to the downstream of the Xijiang River. Further analysis demonstrated that the discharge of waste containing CBs may be the main source of CBs pollution in the Xijiang River .

Damage and Control of Poisonous Weeds in Western Grassland of China

Western grassland is the main source of living and means of production of western inhabitants. For many years, desertification and poisonous-weeds growth in grassland were resulted from over-grazing, over-reclaiming, over-spading and population growth. Western natural ecological environment is destroyed severly. Meanwhile, it has restricted the sustainable development of animal husbandry. The fast spreading poisonous-weeds, which caused grassland ecology unbalance, is one of the considerable bioecology problems and an important index of grassland degeneration. Based on analysis and induction of previous data, this article introduced the situation of poisonous-weeds disaster of western grassland in recent decades, category and distribution of poisonous-weeds, integrated control and reasonable utilization.

Control Efficiency and Crop Safety of 20% Cyhalofop-butyl WP on Grass Weeds in Direct Seeded Rice Fields

The purpose was to define the control efficiency and safety of 20% cy- halofop-butyl WP on grass weeds in direct seeded rice fields, in order to provide the basis for chemical weeding. This study measured Leptochloa chinensis（L.） Nees, Echinoch/oa crusgalli （L.） Beauv and other gramineous weed control efficiency with four concentrations of 20% cyhalofop-dutyl WP and 100 g/L cyhalofop-dutyl EC in direct seeded rice fields, and analyzed the yield-increasing effect and safety of rice. The results showed that 20% cyhalofop-butyl WP had a good control efficiency on grass weeds such as Leptochloa chinensis（L.） Nees, Echinoch/oa crusgalli（L.） Beauv and other grasses. The effective dosage of 90-150 g/hm2 was over 90.7% on Lep- foch/oa chinensis（L.） Nees and the comprehensive control effect of the grass weeds was above 86.7%, which was basically consistent with 100 g/L cyhalofop-dutyl EC. Furthermore, 20% cyhalofop-dutyl WP was high security for direct seeded rice fields. The yield of rice was increased by 10.18%-11.22% after spraying herbicide. There- fore, 20% of cyhalofop-dutyl WP can be used as a special herbicide for controlling Leptochloa chinensis（L.） Beauv in direct seeded rice fields, and has a good applica- tion prospect.

Development of Transgenic Glyphosate-Resistant Rice with G6 Gene Encoding 5-Enolpyruvylshikimate-3-Phosphate Synthase

Glyphosate-resistant crops have been a huge economic success for genetic engineering. The creating of new glypbosateresistant plants would increase the available choices for planting and lower the price of genetically modified crop seeds. A novel G6 gene from Pseudomonas putida that encoded 5-enolpyruvylshikimate-3-phosphate synthase （EPSPS） was previously isolated. The G6 gene was transfected into rice via Agrobacterium-mediated transformation. The transgenic rice obtained was confirmed by PCR, Southern, and Western blots. The lab experiment and field trials further confirmed that the transgenic rice can survive glyphosate spraying at a dose of 8 g L^-1. In contrast, conventional rice was killed at a weed control glyphosate spray dose of 1 g L^-1. Altogether, the present study showed that the G6 gene works well in rice in vivo for glyphosate-resistance.