INHIBITION OF PROLIFERATION OF HUMAN BREAST CANCER MCF-7 CELLS BY SMALL INTERFERENCE RNA AGAINST LRP16 GENE

Objective: Our previous studies have firstly demonstrated that 17β -E2 up-regulates LRP16 gene expression in human breast cancer MCF-7 cells, and ectopic expression of the LRP16 gene promotes MCF-7 cells proliferation. Here, the effects of the LRP16 gene expression on growth of MCF-7 human breast cancer cells and the mechanism were further studied by establishing two stably LRP16-inhibitory MCR-7 cell lines. Methods: Hairpin small interference RNA (siRNA) strategy, by which hairpin siRNA was released by U6 promoter and was mediated by pLPC-based retroviral vector, was adopted to knockdown endogenous LRP16 level in MCF-7 cells. And the hairpin siRNA against green fluorescence protein (GFP) was used as the negative control. The suppressant efficiency of the LRP16 gene expression was confirmed by Nothern blot. Cell proliferation assay and soft agar colony formation assay were used to determine the status of the cells proliferation. Cell cycle checkpoints including cyclin E and cyclin D1 were examined by Western blot. Results: The results from cell proliferation assays suggested that down-regulation of LRP16 gene expression is capable of inhibiting MCF-7 breast cancer cell growth and down-regulation of the LRP16 gene expression is able to inhibit anchorage-independent growth of breast cancer cells in soft agar. We also demonstrated that cyclin E and cyclin D1 proteins were much lower in the LRP16-inhibitory cells than in the control cells. Conclusion: These data suggest that LRP16 gene play an important role in MCF-7 cells proliferation by regulating the pathway of the G1/S transition and may function as an important modulator in regulating the process of tumorigenesis in human breast.

F-interference law generation and its feature recognition

By using two-directional S-rough sets, the concepts of （f, f）-interference generation and （f, f）- interference law generation of knowledge, F-interference generation and F-interference law generation of two-directional S-rough sets are proposed. Based on the concepts above, the relation theoreras between F-interference loss and F-interference degree, the relation theorems between F-interference loss law and F-interference degree law, the dis- cernibility theorems between F-interference and F-interference law are presented. At last, the recognition criterion of F-interference law and its application are given.

Second-order interference of two independent and tunable single-mode continuous-wave lasers

The second-order temporal interference of two independent single-mode continuous-wave lasers is discussed by em- ploying two-photon interference in Feynman＇s path integral theory. It is concluded that whether the second-order temporal interference pattern can or cannot be retrieved via two-photon coincidence counting rate is dependent on the resolution time of the detection system and the frequency difference between these two lasers. Two identical and tunable single-mode continuous-wave diode lasers are employed to verify the predictions. These studies are helpful to understand the physics of two-photon interference with photons of different spectra.

50 Gbit/s前传光模块关键技术研究与应用展望

随着全球5G网络的规模化商用及移动互联网流量的迅猛增长,更为丰富的无线频谱资源得以释放,高速率光模块在无线前传网络中的需求日益凸显。聚焦于50 Gbit/s前传光模块的关键技术,深入剖析了前传网络的两种主要组网方式,分析了前传链路的典型传输距离及链路损耗情况,探讨了10 km场景下粗波分复用(coarse wavelength division multiplex,CWDM)中各波长的最大色散值和最小色散值,并研究了相应的色散补偿策略,通过实验验证了不同多径干扰(multi-path interference,MPI)强度下系统光功率代价的变化,详细分析了多径干扰噪声对系统误码性能的潜在影响,为前传光模块参数的优化提供了重要的理论依据。此外,深入研究了50 Gbit/s前传光模块的光电芯片技术方案,对多种不同类型光模块的参数性能进行了严格的测试,提出了针对50 Gbit/s前传光模块光电接口参数的标准化建议,详细介绍了当前50 Gbit/s前传光模块在国内外的标准现状,并展望了未来应用的发展趋势。

Government's Responsibility for Control of Agricultural Environmental Pollution in China

In this paper,the current situation of agricultural environmental pollution caused by the increase of chemicals input in agriculture in China was analyzed,and it is found that agricultural pollution is related to agricultural industrial policies,urban-rural economic structure,funds input in pollution control,comprehensive environmental management,laws of pollution control,and so forth. To control agricultural pollution effectively,it is needed to implement integration of agricultural and environmental policies,establish environmentally friendly agricultural technology popularizing system,implement integrated planning and management of a basin,and set up and improve legislation to protect agricultural environment.

The Impact of Government Supervision and Consumer Purchasing Behavior on Food Safety

This paper aims at the theoretical analysis to the impact of government supervision and consumer purchasing behavior on food quality security, so as to look for safety strategies and measures to strengthen and improve the level of food safety in China. Reputation mechanism is introduced and Bayesian approach is based on, in which government supervision as well as consumer purchasing behavior is taken as crucial factors to impact on the food quality security. As to the proposed quantitative indicators, government supervision includes exposure rate, fine and etc.;at the same time, consumer purchasing behavior includes consumer’s WTP for security food and consumer expectations to food safety. Taking China’s dairy industry as an example, it makes simulation by Netlog. The results show that consumer purchasing behavior alone has little effect on the dairy companies’ decision-making to be honest or counterfeiting enterprises. However, combination government supervision with purchasing behavior has great impact, and plays very good effects on food safety.

Second-order temporal interference of two independent light beams at an asymmetrical beam splitter

The second-order temporal interference of classical and nonclassical light at an asymmetrical beam splitter is discussed based on two-photon interference in Feynman＇s path integral theory. The visibility of the second-order interference pattern is determined by the properties of the superposed light beams, the ratio between the intensities of these two light beams, and the reflectivity of the asymmetrical beam splitter. Some requirements about the asymmetrical beam splitter have to be satisfied in order to ensure that the visibility of the second-order interference pattern of nonclassical light beams exceeds the classical limit. The visibility of the second-order interference pattern of photons emitted by two independent single-photon sources is independent of the ratio between the intensities. These conclusions are important for the researches and applications in quantum optics and quantum information when an asymmetrical beam splitter is employed.

On Russia's Government Shakeup

On March 23,1998,Russian President Boris Yeltsin stunned the nation by dis-missing the government of Prime Minister Viktor Chernomyrdin.Then henominated Mr.Sergei Kiriyenko,35,who had been OD the energy minister postfor only three months,for the new head of the cabinet.This immediately ignited afierce political war of a month long between the executive and the legislature draw-ing great concern from the international community.At the moment when

The Transformation of Environmental Governance from the Point of the Interaction between Government and National People's Congress

Taking cadmium pollution in X River as an example, the interaction between National People＇s Congress and local government was de- scribed. How the local government transforms its role in environment protection with the help of several deputies to National People＇s Congress was expounded from the angle of social construction, which also enlightened the way to build an environment protection road with Chinese characteristics.

On the Government's Responsibility in China's Rural Land Transfer

There is an urgent need for China's rural land transfer in order to develop modern agriculture,ensure food security,transfer rural population,and accelerate industrialization and urbanization.At present,there is still disordered transfer of rural land in China,making the interests of the transferors and transferees difficult to be effectively guaranteed;there is inefficient transfer,leading to decline in actual agricultural production level after transfer;there is jobless transfer,leading to difficult placement for rural surplus labor after transfer,seriously restricting rural land transfer.Therefore,the Chinese government's legal responsibility,economic responsibility and social responsibility in rural land transfer should be further strengthened.

S-rough Laws and Its Dependent Separation by F-Interference

By employing function S-rough sets,the concepts of f-interference generation and separation of law,and the F-interference generation and separation of S-rough laws were proposed. Base on the concepts above,the F-interference relation theorems,the F-interference discernibility theorem,the dependent separation theorem by interfereence,and the dependent separation principle by interference were presented; finally the application was given. By employing F-interference dependence and separation of S-rough laws,the changes of characteristic of dynamic systemic regularities could be recognized.

Interference Experiment with a Transparent Mask Rejects Wave Models of Light

Young’s double-slit experiment shows characteristics of light that are modeled as indicating a wave nature of light. Other experiments suggest a particle model of light. An experiment is performed with the Fraunhofer pattern from a first mask impinging on a transparent second mask with a slit. The screen pattern is an interference pattern such as produced in Young’s Experiment. An opaque strip between the first and second mask blocks the light of the center maxima from the first mask. The screen interference fringes remained. Moving the glass mask so the slit is removed from light shows the necessity of a slit in the second mask. This suggests a Newtonian type of light model. This experiment rejects the wave models of light.

The path and countermeasures of increasing government’s governance capacities in energy-saving renovation of existing buildings

The dual functions of government participation and governance in the development of the existing building energy-saving renovation market determine the importance of the government’s scientific and effective actions.Continuously improving the government’s role is to promote the effective operation of the existing building energy-saving renovation market.Based on the inherent requirements of improving the government governance capacity in the development of the existing building energy-saving renovation market,this paper constructs the implementation path for the development of the existing building’s energy-saving renovation market from the three dimensions of practical concept,practical process and practical methods.From the four aspects of improving the legal and regulatory system,building a market information sharing platform,establishing a sound market supervision mechanism,and consolidating and improving the market accountability system,we will structure the existing building energysaving renovation market development to improve the government’s ability to restrain and implement measures.From the four levels of improving the market incentive mechanism,optimizing the market financing environment,clarifying the position of market resource allocation,and building a social operation platform,design measures to improve government incentives in the development of the existing building energy conservation renovation market.From the shaping of governance yuan organizational roles,governance capacity and responsibilities of departments at all levels to define boundaries,to build four main interactive platform governance perspective,the main parties demand balance the interests of both the governance capacity development planning in the government market,building energy-saving market to enhance the implementation of countermeasures.In order to promote the healthy and sustainable development of the existing building energy-saving renovation market based on the enhancement of government governance capacity.

ANHUI PROVINCIAL PEOPLE'S GOVERNMENT DECIDES TO SPEED UP THE USE OF FOREIGN CAPITAL

On February 12,the Anhui ProvincialPeople’s Government made a decisionon the speeding up of the use of foreigncapital.The decision involved the followingseven aspects: 1.To seize the opportunity to reviseideology and to open up a new situation formaking use of foreign capital.The decisionurges the various localities to seize the righttime to shift their focus to making good useof their own advantages,and improving thelocal investment climate and the quality ofservice in the light of their actual conditionsso as to absorb more foreign funds.Meanwhile,enterprises must be led to face the marketand to join equal competition so as to pushforward the use of foreign funds to a

First Batch of Anhui Brandname Products Recommended by Anhui Provincial People's Government

Product NameMagang brand high quality carbon steel,common low carbon steel-made cold/hotrolling coil rod(without twisting control)Magang brand integral roll steel-madepassenger train wheel(φ915mm) forrailway useJingjing brand electrolytic

Four Bases of Xi Jinping's Thoughts on the Government of China

Since the 18th CPC National Congress, the CPC Central Committee, led by General Secretary Xi Jinping, has unswervingly adhered to the continuing development of socialism with Chinese characteristics. Through courageous practice and excellent innovation, the CPC Central Committee has shaped a series of new ideas, thoughts and strategies of state governing, which serve as scientific guidelines for the actions and theories on further deepening the Reform and Opening-up and accelerating the modernization of socialism in China. Compared with "new ideas and new strategies," the "new thoughts" are more extensive, inclusive and thorough. In fact, "new thoughts" include "new ideas" and "new strategies" and are demonstrated by the latter two. Presenting the new CPC Central Committee's achievements in both practical and theoretical innovations with Xi Jinping's governing thought as the guide can further promote and deepen the essence of General Secretary Xi Jinping's series of key speeches. Xi Jinping's governing thought has its historical basis, realistic basis, theoretical basis and political basis.

ON BRITISH LABOUR GOVERNMENT'S HONG KONG POLICY

Hong Kong’s return to China is a historic event in world affairs as well as inSino-British relations.Britain’s policy toward Hong Kong,therefore,has aninternational significance.Immediately after the British election in early May,theworld press were generally of the view that the Labour’s victory would make littleor no difference to the Hong Kong handover question.The appraisal still holdslargely true today.Judging from new developments since then,however,there

养老服务中的政府供给研究——基于S省养老服务政策的内容分析

采用内容分析法确定政府供给养老服务的五个维度:养老服务制度规划、养老服务财政支持、生活照料养老服务、养老设施服务、信息化养老服务。结合历年统计年鉴及政府公开数据对以上维度进行分析,发现政府的养老服务制度规划趋向完善和多元,养老服务财政支持扩张与紧缩并存,生活照料养老服务供给有限,养老设施服务供给重点从机构走向社区,信息化养老服务供给以平台建设为主。

Chinese Government's Progress in Solving Y2K Bug

Theyear2000isapproaching.Thecomputerproblemin2000islikeatimebomb,andthedateforitsexplosionisapproaching.Eachcountryismakingeffortstosolvethisproblem,andsoistheChinesegovernment.Asearlyas1996,theStateScienceCommission,theformerMinistryofElectronicsand...

Research of the Experience of Western Governments Procurement of Public Service

Government procurement of public services is developed from the market-oriented reform of public services in Western countries.Many Western countries have adopted the practice of reform and have gained success of this model.By analyzing the process of those in UK,the Netherlands,the United States and France,summing up experience,and I hope that’s useful for China’s reform.