A headspace solid-phase microextraction-gas chromatography-mass spectrometry(HS-SPME/GC-MS)method was used to study the volatile organic compounds(VOCs)associated with the differential immune response of tomato plants...A headspace solid-phase microextraction-gas chromatography-mass spectrometry(HS-SPME/GC-MS)method was used to study the volatile organic compounds(VOCs)associated with the differential immune response of tomato plants infected with the recombinant strain of potato virus Y(PVY^(C)-to),necrogenic to tomato.Analysis was carried out in UC82(UC),a virus susceptible tomato variety,comparing the same UC plants grafted or not onto a virus tolerant tomato ecotype,Manduria(Ma);the three types of samples used for the GC-MS analysis were mock-inoculated UC/Ma plants,UC/Ma+PVY^(C)-to and UC+PVY^(C)-to plants;the VOCs obtained were 111.Results from symptomatic PVY^(C)-to-infected UC plants showed a VOCs composition enriched in alcohols,fatty acid derivates,benzenoids,and salicylic acid derivatives,while in mock-inoculated UC/Ma plants VOCs were mainly characterized by methyl ester compounds.The VOC profile was in line with RNAseq data analyses,denoting that PVY^(C)-to viral RNA accumulation and disease symptoms induce the specific transcriptional activation of genes involved in VOCs biosynthesis.Furthermore,principal component analysis highlighted that VOCs of PVY^(C)-to-infected and mock-inoculated grafted plants were much closer each other than that of symptomatic PVY^(C)-to-infected non-grafted UC plants.These results suggest that VOCs profiles of tomato plants are related to the viral RNA accumulation,disease intensity and graft-derived tolerance to PVY^(C)-to infection.展开更多
People have grafted plants since antiquity for propagation,to increase yields,and to improve stress tolerance.This cutting and joining of tissues activates an incredible regenerative ability as different plants fuse a...People have grafted plants since antiquity for propagation,to increase yields,and to improve stress tolerance.This cutting and joining of tissues activates an incredible regenerative ability as different plants fuse and grow as one.For over a hundred years,people have studied the scientific basis for how plants graft.Today,new techniques and a deepening knowledge of the molecular basis for graft formation have allowed a range of previously ungraftable combinations to emerge.Here,we review recent developments in our understanding of graft formation,including the attachment and vascular formation steps.We analyze why plants graft and how biotic and abiotic factors influence successful grafting.We also discuss the ability and inability of plants to graft,and how grafting has transformed both horticulture and fundamental plant science.As our knowledge about plant grafting improves,new combinations and techniques will emerge to allow an expanded use of grafting for horticultural applications and to address fundamental research questions.展开更多
<i><span style="font-family:"">Jatropha</span></i><span style="font-family:""> <i>platyphylla</i> </span><span style="font-family:&...<i><span style="font-family:"">Jatropha</span></i><span style="font-family:""> <i>platyphylla</i> </span><span style="font-family:"">is considered as a potential source of edible protein, oil, and phenolic compounds with anti-inflammatory activity. The use of stem cutting in vegetative propagation and grafting is as indispensable tools for mass multiplication of superior genotypes and helps in improve planting yield and quality. The study was aimed to investigate the effect of different diameters (10 - 15, 16 - 25 and 26 - 35 mm) and different hormone concentrations of indo-butyric acid (0, 1.5, 3, 6 and 10 g/L), in the rooting of <i>Jatropha</i> <i>platyphylla</i> and to obtain axillary buds to performed grafts. Rooting efficiency was 80% in greenhouse conditions. Hormone concentration and diameter significantly affected the rooting and shooting ability of <i>Jatropha</i> <i>platyphylla</i> stem cuttings. Stem cuttings of 26 - 35 mm have the greatest number, length and dry root weight. 76% survival of the grafted plants was obtained. <span>This demonstrates the necessity to improve the conventional propagation methods</span> with appropriate scientific inputs for more economical and time efficient techniques for standard propagation protocols.展开更多
The six years walnut（Juglans regia）cultivars （grafting plant） and three years seedlings were used as material to study the change of the endogenous hormone contents in roots and buds and the correlation with growt...The six years walnut（Juglans regia）cultivars （grafting plant） and three years seedlings were used as material to study the change of the endogenous hormone contents in roots and buds and the correlation with growth vigor of walnut in the same year. The results indicated that the endogenous hormone contents in bud of different walnut cultivars had insignificant correlation with the growth vigor. The GA3 content in roots of walnut seedlings had positive significant correlation with tree height and its increment. The ABA content in roots of walnut seedlings had strikingly negative relationship with tree height and its increment, and trunk girth. The GA3 content in buds of walnut seedlings had positive correlation significantly with the increment of tree height and the ABA content in buds of walnut seedling had strikingly negative relationship with tree height and its increment. The IAA content in roots of walnut seedlings had bally positive correlation significantly with that in buds.展开更多
基金funding from the European Union Next Generation EU(PIANO NAZIONALE DI RIPRESA E RESILIENZA(PNRR)–MISSIONE 4 COMPONENTE 2,INVESTIMENTO 1.4–D.D.103217/06/2022,CN00000022)。
文摘A headspace solid-phase microextraction-gas chromatography-mass spectrometry(HS-SPME/GC-MS)method was used to study the volatile organic compounds(VOCs)associated with the differential immune response of tomato plants infected with the recombinant strain of potato virus Y(PVY^(C)-to),necrogenic to tomato.Analysis was carried out in UC82(UC),a virus susceptible tomato variety,comparing the same UC plants grafted or not onto a virus tolerant tomato ecotype,Manduria(Ma);the three types of samples used for the GC-MS analysis were mock-inoculated UC/Ma plants,UC/Ma+PVY^(C)-to and UC+PVY^(C)-to plants;the VOCs obtained were 111.Results from symptomatic PVY^(C)-to-infected UC plants showed a VOCs composition enriched in alcohols,fatty acid derivates,benzenoids,and salicylic acid derivatives,while in mock-inoculated UC/Ma plants VOCs were mainly characterized by methyl ester compounds.The VOC profile was in line with RNAseq data analyses,denoting that PVY^(C)-to viral RNA accumulation and disease symptoms induce the specific transcriptional activation of genes involved in VOCs biosynthesis.Furthermore,principal component analysis highlighted that VOCs of PVY^(C)-to-infected and mock-inoculated grafted plants were much closer each other than that of symptomatic PVY^(C)-to-infected non-grafted UC plants.These results suggest that VOCs profiles of tomato plants are related to the viral RNA accumulation,disease intensity and graft-derived tolerance to PVY^(C)-to infection.
基金supported by a European Research Council starting grant(GRASP-805094)supported by an MSCA Postdoctoral Fellowship(UMOCELF-101069157).
文摘People have grafted plants since antiquity for propagation,to increase yields,and to improve stress tolerance.This cutting and joining of tissues activates an incredible regenerative ability as different plants fuse and grow as one.For over a hundred years,people have studied the scientific basis for how plants graft.Today,new techniques and a deepening knowledge of the molecular basis for graft formation have allowed a range of previously ungraftable combinations to emerge.Here,we review recent developments in our understanding of graft formation,including the attachment and vascular formation steps.We analyze why plants graft and how biotic and abiotic factors influence successful grafting.We also discuss the ability and inability of plants to graft,and how grafting has transformed both horticulture and fundamental plant science.As our knowledge about plant grafting improves,new combinations and techniques will emerge to allow an expanded use of grafting for horticultural applications and to address fundamental research questions.
文摘<i><span style="font-family:"">Jatropha</span></i><span style="font-family:""> <i>platyphylla</i> </span><span style="font-family:"">is considered as a potential source of edible protein, oil, and phenolic compounds with anti-inflammatory activity. The use of stem cutting in vegetative propagation and grafting is as indispensable tools for mass multiplication of superior genotypes and helps in improve planting yield and quality. The study was aimed to investigate the effect of different diameters (10 - 15, 16 - 25 and 26 - 35 mm) and different hormone concentrations of indo-butyric acid (0, 1.5, 3, 6 and 10 g/L), in the rooting of <i>Jatropha</i> <i>platyphylla</i> and to obtain axillary buds to performed grafts. Rooting efficiency was 80% in greenhouse conditions. Hormone concentration and diameter significantly affected the rooting and shooting ability of <i>Jatropha</i> <i>platyphylla</i> stem cuttings. Stem cuttings of 26 - 35 mm have the greatest number, length and dry root weight. 76% survival of the grafted plants was obtained. <span>This demonstrates the necessity to improve the conventional propagation methods</span> with appropriate scientific inputs for more economical and time efficient techniques for standard propagation protocols.
文摘The six years walnut（Juglans regia）cultivars （grafting plant） and three years seedlings were used as material to study the change of the endogenous hormone contents in roots and buds and the correlation with growth vigor of walnut in the same year. The results indicated that the endogenous hormone contents in bud of different walnut cultivars had insignificant correlation with the growth vigor. The GA3 content in roots of walnut seedlings had positive significant correlation with tree height and its increment. The ABA content in roots of walnut seedlings had strikingly negative relationship with tree height and its increment, and trunk girth. The GA3 content in buds of walnut seedlings had positive correlation significantly with the increment of tree height and the ABA content in buds of walnut seedling had strikingly negative relationship with tree height and its increment. The IAA content in roots of walnut seedlings had bally positive correlation significantly with that in buds.
