A promising therapeutic strategy to promote the regeneration of injured axons in the adult central nervous system（CNS）is the transplantation of cells or tissues that can modify the local host environment and support...A promising therapeutic strategy to promote the regeneration of injured axons in the adult central nervous system（CNS）is the transplantation of cells or tissues that can modify the local host environment and support the growth of regenerating axons.展开更多
Objective: To determine whether Schwann cells (SCs) and fibronectin (FN) support the growth of damaged axons and their conductive function. Methods: Thirty adult Sprague Dawley (SD) rats were randomized into 3 groups ...Objective: To determine whether Schwann cells (SCs) and fibronectin (FN) support the growth of damaged axons and their conductive function. Methods: Thirty adult Sprague Dawley (SD) rats were randomized into 3 groups (n=10), and the following grafts: both SCs and FN, FN, and basal medium were implanted respectively into the lumbar of spinal cords hemisected at vertebra T 12 . Results: At 6 weeks postoperation, the latencies of spinal cord evoked potential (SCEP) P 1 wave in the three groups were 1.57 ms, 1.84 ms, and 2.03 ms, respectively. The differences between SCs/FN group and the other two groups were statistically significant. The number of regenerated axons in SCs/FN group was significantly greater than that in FN group. The number of survival neurons in L 4 and L 5 left dorsal root ganglia (DRG) in SCs/FN group was significantly greater than that in the rest two groups respectively. The latencies of P 1 wave were significantly correlated with axon counts in both SCs/FN and FN groups. Conclusions: SCs/FN grafted to the hemisected lumbar of spinal cords can produce robust axon regeneration and promote partial repair of their conduction. Surface recording of SCEP technique has been proved to be a reliable and less traumatic method for assessment of recovery of afferent conduction in hemisected spinal cord.展开更多
基金supported by NIH NS055976Craig H.Neilsen Foundation 280850
文摘A promising therapeutic strategy to promote the regeneration of injured axons in the adult central nervous system（CNS）is the transplantation of cells or tissues that can modify the local host environment and support the growth of regenerating axons.
文摘Objective: To determine whether Schwann cells (SCs) and fibronectin (FN) support the growth of damaged axons and their conductive function. Methods: Thirty adult Sprague Dawley (SD) rats were randomized into 3 groups (n=10), and the following grafts: both SCs and FN, FN, and basal medium were implanted respectively into the lumbar of spinal cords hemisected at vertebra T 12 . Results: At 6 weeks postoperation, the latencies of spinal cord evoked potential (SCEP) P 1 wave in the three groups were 1.57 ms, 1.84 ms, and 2.03 ms, respectively. The differences between SCs/FN group and the other two groups were statistically significant. The number of regenerated axons in SCs/FN group was significantly greater than that in FN group. The number of survival neurons in L 4 and L 5 left dorsal root ganglia (DRG) in SCs/FN group was significantly greater than that in the rest two groups respectively. The latencies of P 1 wave were significantly correlated with axon counts in both SCs/FN and FN groups. Conclusions: SCs/FN grafted to the hemisected lumbar of spinal cords can produce robust axon regeneration and promote partial repair of their conduction. Surface recording of SCEP technique has been proved to be a reliable and less traumatic method for assessment of recovery of afferent conduction in hemisected spinal cord.
