Estimating wheat grain protein content by remote sensing is important for assessing wheat quality at maturity and making grains harvest and purchase policies. However, spatial variability of soil condition, temperatur...Estimating wheat grain protein content by remote sensing is important for assessing wheat quality at maturity and making grains harvest and purchase policies. However, spatial variability of soil condition, temperature, and precipitation will affect grain protein contents and these factors usually cannot be monitored accurately by remote sensing data from single image. In this research, the relationships between wheat protein content at maturity and wheat agronomic parameters at different growing stages were analyzed and multi-temporal images of Landsat TM were used to estimate grain protein content by partial least squares regression. Experiment data were acquired in the suburb of Beijing during a 2-yr experiment in the period from 2003 to 2004. Determination coefficient, average deviation of self-modeling, and deviation of cross- validation were employed to assess the estimation accuracy of wheat grain protein content. Their values were 0.88, 1.30%, 3.81% and 0.72, 5.22%, 12.36% for 2003 and 2004, respectively. The research laid an agronomic foundation for GPC (grain protein content) estimation by multi-temporal remote sensing. The results showed that it is feasible to estimate GPC of wheat from multi-temporal remote sensing data in large area.展开更多
Grain protein content(GPC)is an indicator of cereal nutritional quality.Identification of genes involved in the regulation of GPC provides targets for molecular breeding for crop protein quality.We characterized a mai...Grain protein content(GPC)is an indicator of cereal nutritional quality.Identification of genes involved in the regulation of GPC provides targets for molecular breeding for crop protein quality.We characterized a maize gene encoding the putative amino acid transporter ZmAAP6,a gene expressed mainly in immature seeds,especially in the basal endosperm transfer layer.Total protein and zein contents were decreased in ZmAAP6 null mutants and increased in ZmAAP6 overexpression(OE)lines,consistent with their changed in the size of protein bodies.Metabolic and transcriptomic analysis supported the regulatory role of ZmAAP6 in amino acid transportation.These results suggest that ZmAAP6 functions as a positive regulator of GPC in maize,shedding new light on the genetic basis of GPC regulation.展开更多
The grain protein content(GPC)is the key parameter for wheat grain nutritional quality.This study conducted a resampling GWAS analysis using 406 wheat accessions across eight environments,and identified four previousl...The grain protein content(GPC)is the key parameter for wheat grain nutritional quality.This study conducted a resampling GWAS analysis using 406 wheat accessions across eight environments,and identified four previously reported GPC QTLs.An analysis of 87 landraces and 259 modern cultivars revealed the loss of superior GPC haplotypes,especially in Chinese cultivars.These haplotypes were preferentially adopted in different agroecological zones and had broad effects on wheat yield and agronomic traits.Most GPC QTLs did not significantly reduce yield,suggesting that high GPC can be achieved without a yield penalty.The results of this study provide a reference for future GPC breeding in wheat using the four identified QTLs.展开更多
On the basis of previous studies dealing with the variation of major agronomic and yield characteristics of regenerated plants derived from single cell culture in vitro of common wheat (Triticum aestivum L.Cult...On the basis of previous studies dealing with the variation of major agronomic and yield characteristics of regenerated plants derived from single cell culture in vitro of common wheat (Triticum aestivum L.Cultivar NE 7742), the grain protein content and its fractions from regenerated plants with stable agronomic characteristics were studied from 1992 to 1995. The results showed that the variation of grain protein content and its fractions in somaclones from single cell culture in vitro were very significant and the range was very wide (11531770%). Several types of variation were found in the studies, especially the type with higher protein content than that of cultivar NE 7742 (non-culture parent). Among them, -2069% of lines the grain protein content was significantly higher than that of NE 7742 and combined with high yielding potential. The tendency of variation of the four protein fractions showed that the variation of albumin was not obvious and maintained the same level as NE774 increased in some somaclones and decreased in others. However, the percentages both globulin and glutenin tended to increase. The variation of total amount of structural protein and the ratio between globulin and glutenin tended to increase. The variation of total amount of structural protein and the ratio between globulin and albumm was mainly influenced by globulin under the condition of culture in vitro. The variation of total amount of storage protein and the ratio between gliadin and glutenin was mainly affected by glutenin. The results mentioned above demonstrated that the induction and screening of somaclonal variation could be an effective way in wheat improvement in combining high protein content with high yield.展开更多
Enhancing the nutritional quality of food crops is a way to improve human nutrition and health. Grain protein content ( GPC ) is an important nutritional quality trait for rice and many other crops. Therefore, it wi...Enhancing the nutritional quality of food crops is a way to improve human nutrition and health. Grain protein content ( GPC ) is an important nutritional quality trait for rice and many other crops. Therefore, it will be very significant to increase GPC and improve rice quality. This paper mainly reviewed the genetic studies GPC in rice, mapping and cloning of QTLs and genes associated with GPC. However, little is known about the molecular mechanism and gene function related to GPC in rice. Thus, here we put forward the genetic strategy by gene pyramiding or molecular marker assisted selection to improve GPC and nutritional quality in rice. This review could provide important information for genetic improvement of grain nutritional quality of rice.展开更多
To maintain high wheat grain yield in areas where frequent and periodic waterlogging occurs,the effects of waterlogging on the photosynthesis,growth,yield,and protein content of three wheat cultivars,namely Xiangmai55...To maintain high wheat grain yield in areas where frequent and periodic waterlogging occurs,the effects of waterlogging on the photosynthesis,growth,yield,and protein content of three wheat cultivars,namely Xiangmai55(X55),Jingmai102(J102),and Zhengmai9023(Z9023),in four different growth stages were investigated.Experiments were conducted in specially designed experimental tanks in the middle and lower reaches of the Yangtze River during the 2012-2013 and 2013-2014 wheat growing seasons in China.Results showed that X55 was the most susceptible to waterlogging,followed by J102 and Z9023.Chlorophyll content reduction and leaf senescence,which resulted in decreased green-to-total leaf number ratio,were induced to the greatest extents by waterlogging in booting and flowering stages,followed by milky stage.Meanwhile,chlorophyll content in flag leaf,plant height were significantly decreased by waterlogging in jointing stage but effectively recovered after waterlogging withdrawal,and recovery ability varied among the cultivars.Plant biomass and grain yield were most significantly decreased by waterlogging in booting and flowering stages,followed by milky and jointing stages.Grain protein content was also considerably affected by waterlogging depending on growth stage and cultivars.The decreased grain yield caused by waterlogging was mostly due to the sharp decline in 1 000-grain mass.Waterlogging led to reduced protein yield in all growth stages in three wheat cultivars.Above all,in this experiment,waterlogging decreased grain yield significantly,and waterlogging at booting stage and flowering stage was most serious.Comparing the three cultivars,X55 was most sensitive to waterlogging.展开更多
A crop growth model,integrating genotype,environment,and management factor,was developed to serve as an analytical tool to study the influence of these factors on crop growth,production,and agricultural planning.A maj...A crop growth model,integrating genotype,environment,and management factor,was developed to serve as an analytical tool to study the influence of these factors on crop growth,production,and agricultural planning.A major challenge of model application is the optimization and calibration of a considerable number of parameters.Sensitivity analysis(SA) has become an effective method to identify the importance of various parameters.In this study,the extended Fourier Amplitude Sensitivity Test(EFAST) approach was used to evaluate the sensitivity of the DSSAT-CERES model output responses of interest to 39 crop genotype parameters and six soil parameters.The outputs for the SA included grain yield and quality(take grain protein content(GPC) as an indicator) at maturity stage,as well as leaf area index,aboveground biomass,and aboveground nitrogen accumulation at the critical process variables.The key results showed that:(1) the influence of parameter bounds on the sensitivity results was slight and less than the impacts from the significance of the parameters themselves;(2) the sensitivity parameters of grain yield and GPC were different,and the sensitivity of the interactions between parameters to GPC was greater than those between the parameters to grain yield;and(3) the sensitivity analyses of some process variables,including leaf area index,aboveground biomass,and aboveground nitrogen accumulation,should be performed differently.Finally,some parameters,which improve the model’s structure and the accuracy of the process simulation,should not be ignored when maturity output as an objective variable is studied.展开更多
基金the National Natural Science Foundation of China (41171281, 40701120)the Beijing Nova Program, China (2008B33)
文摘Estimating wheat grain protein content by remote sensing is important for assessing wheat quality at maturity and making grains harvest and purchase policies. However, spatial variability of soil condition, temperature, and precipitation will affect grain protein contents and these factors usually cannot be monitored accurately by remote sensing data from single image. In this research, the relationships between wheat protein content at maturity and wheat agronomic parameters at different growing stages were analyzed and multi-temporal images of Landsat TM were used to estimate grain protein content by partial least squares regression. Experiment data were acquired in the suburb of Beijing during a 2-yr experiment in the period from 2003 to 2004. Determination coefficient, average deviation of self-modeling, and deviation of cross- validation were employed to assess the estimation accuracy of wheat grain protein content. Their values were 0.88, 1.30%, 3.81% and 0.72, 5.22%, 12.36% for 2003 and 2004, respectively. The research laid an agronomic foundation for GPC (grain protein content) estimation by multi-temporal remote sensing. The results showed that it is feasible to estimate GPC of wheat from multi-temporal remote sensing data in large area.
基金supported by the 2022 Research Program of Sanya Yazhou Bay Science and Technology City(SYND-2022-10 to Wei Huang and SYND-2022-03 to Weiwei Jin)。
文摘Grain protein content(GPC)is an indicator of cereal nutritional quality.Identification of genes involved in the regulation of GPC provides targets for molecular breeding for crop protein quality.We characterized a maize gene encoding the putative amino acid transporter ZmAAP6,a gene expressed mainly in immature seeds,especially in the basal endosperm transfer layer.Total protein and zein contents were decreased in ZmAAP6 null mutants and increased in ZmAAP6 overexpression(OE)lines,consistent with their changed in the size of protein bodies.Metabolic and transcriptomic analysis supported the regulatory role of ZmAAP6 in amino acid transportation.These results suggest that ZmAAP6 functions as a positive regulator of GPC in maize,shedding new light on the genetic basis of GPC regulation.
基金supported by the“Integration of Two Chains”Key Research and Development Projects of Shaanxi Province“Wheat Seed Industry Innovation Project”,Chinathe Key R&D of Yangling Seed Industry Innovation Center,China(Ylzy-xm-01)。
文摘The grain protein content(GPC)is the key parameter for wheat grain nutritional quality.This study conducted a resampling GWAS analysis using 406 wheat accessions across eight environments,and identified four previously reported GPC QTLs.An analysis of 87 landraces and 259 modern cultivars revealed the loss of superior GPC haplotypes,especially in Chinese cultivars.These haplotypes were preferentially adopted in different agroecological zones and had broad effects on wheat yield and agronomic traits.Most GPC QTLs did not significantly reduce yield,suggesting that high GPC can be achieved without a yield penalty.The results of this study provide a reference for future GPC breeding in wheat using the four identified QTLs.
基金Project supported by the National Natural Science Foundation of China (No.30400278)National High Technology Research and Development Program (863 Program) of China (No.2006AA10Z129)
文摘用一个裂口阴谋的一个地实验使随机化有三复制的完全的块设计被执行决定在之间的关系光谱索引和小麦谷物产量( GY ),为 GY 比较四个植被索引(力)的表演预言,并且到学习,到估计谷物蛋白质的 VI 的可行性满足( GPC )在里面冬小麦。二典型冬小麦( Triticum aestivum L.)栽培变种 ‘X uzhou 26'(高蛋白质内容)和 ‘H uaimai 18'(低蛋白质内容)被用作著处理和四 N 率,即, 0 , 120 , 210 ,和 300 kg N 哈? 1 ,作为次要情节处理。增加的土壤 N 供应显著地增加了 GY 和 GPC (P ≤ 0.05 ) 。为联合的二栽培变种,重要并且正相关在四个力和 GY 之间被发现,当使用绿比率植被索引(GRVI ) 在时,观察的最强壮的关系地中间充满。累积 VI 实质地估计改进产量预测,与正在出发到成熟舞台的最好的间隔。类似的结果在 VI 和谷物蛋白质产量之间被发现。然而,当使用累积 VI 时, GPC 没显示出重要改进。在叶 N 地位和 GPC 之间的强壮的关系(R2 = 0.9144 为‘ X uzhou 26' 和 R2 = 0.8285 为‘ H uaimai 18') 显示华盖系列能被用来预言 GPC。强壮在估计并且观察的 GPC 之间合适(R2 = 0.7939 ) 显示遥感技术在小麦是谷物蛋白质内容和质量的潜在地有用的预言者。
文摘On the basis of previous studies dealing with the variation of major agronomic and yield characteristics of regenerated plants derived from single cell culture in vitro of common wheat (Triticum aestivum L.Cultivar NE 7742), the grain protein content and its fractions from regenerated plants with stable agronomic characteristics were studied from 1992 to 1995. The results showed that the variation of grain protein content and its fractions in somaclones from single cell culture in vitro were very significant and the range was very wide (11531770%). Several types of variation were found in the studies, especially the type with higher protein content than that of cultivar NE 7742 (non-culture parent). Among them, -2069% of lines the grain protein content was significantly higher than that of NE 7742 and combined with high yielding potential. The tendency of variation of the four protein fractions showed that the variation of albumin was not obvious and maintained the same level as NE774 increased in some somaclones and decreased in others. However, the percentages both globulin and glutenin tended to increase. The variation of total amount of structural protein and the ratio between globulin and glutenin tended to increase. The variation of total amount of structural protein and the ratio between globulin and albumm was mainly influenced by globulin under the condition of culture in vitro. The variation of total amount of storage protein and the ratio between gliadin and glutenin was mainly affected by glutenin. The results mentioned above demonstrated that the induction and screening of somaclonal variation could be an effective way in wheat improvement in combining high protein content with high yield.
基金Supported by National Natural Science Foundation of China(U1604110,U1404319,31270727,31600992)Key Project of Science and Technology in Henan Province(152102110100,152102110036)+5 种基金Nanhu Scholars Program for Young Scholars of XYNU(2016054)Major Science and Technology Project in Henan Province(121100110200)Science and Technology Innovation Team in Henan Province(14IRTSTHN012)Key Research Projects of Henan Higher Education Institutions(15A180059)Students Innovation and Training Program of XYNU(201710477032)Institute for Conservation and Utilization of Agrobioresources in Dabie Mountains(2016020)
文摘Enhancing the nutritional quality of food crops is a way to improve human nutrition and health. Grain protein content ( GPC ) is an important nutritional quality trait for rice and many other crops. Therefore, it will be very significant to increase GPC and improve rice quality. This paper mainly reviewed the genetic studies GPC in rice, mapping and cloning of QTLs and genes associated with GPC. However, little is known about the molecular mechanism and gene function related to GPC in rice. Thus, here we put forward the genetic strategy by gene pyramiding or molecular marker assisted selection to improve GPC and nutritional quality in rice. This review could provide important information for genetic improvement of grain nutritional quality of rice.
基金Supported by the National Science Foundation of China(31371580)
文摘To maintain high wheat grain yield in areas where frequent and periodic waterlogging occurs,the effects of waterlogging on the photosynthesis,growth,yield,and protein content of three wheat cultivars,namely Xiangmai55(X55),Jingmai102(J102),and Zhengmai9023(Z9023),in four different growth stages were investigated.Experiments were conducted in specially designed experimental tanks in the middle and lower reaches of the Yangtze River during the 2012-2013 and 2013-2014 wheat growing seasons in China.Results showed that X55 was the most susceptible to waterlogging,followed by J102 and Z9023.Chlorophyll content reduction and leaf senescence,which resulted in decreased green-to-total leaf number ratio,were induced to the greatest extents by waterlogging in booting and flowering stages,followed by milky stage.Meanwhile,chlorophyll content in flag leaf,plant height were significantly decreased by waterlogging in jointing stage but effectively recovered after waterlogging withdrawal,and recovery ability varied among the cultivars.Plant biomass and grain yield were most significantly decreased by waterlogging in booting and flowering stages,followed by milky and jointing stages.Grain protein content was also considerably affected by waterlogging depending on growth stage and cultivars.The decreased grain yield caused by waterlogging was mostly due to the sharp decline in 1 000-grain mass.Waterlogging led to reduced protein yield in all growth stages in three wheat cultivars.Above all,in this experiment,waterlogging decreased grain yield significantly,and waterlogging at booting stage and flowering stage was most serious.Comparing the three cultivars,X55 was most sensitive to waterlogging.
基金supported by grants from the National Program on the Development of Basic Research (Grant No. 2011CB100200)the National Program of High Technology Development (Grant No. 2010AA101801)+1 种基金the National Program of Plant Transgenic Breeding (Grant No. 2008ZX08009-003)the National Natural Science Foundation of China (Grant No. 30671114)
基金supported by the National Natural Science Foundation of China(41701375,41601369,and 41471285)the European Space Agency(ESA)and Ministry of Science and Technology of China(MOST)Dragon 4 Cooperation Programme(32275-1)
文摘A crop growth model,integrating genotype,environment,and management factor,was developed to serve as an analytical tool to study the influence of these factors on crop growth,production,and agricultural planning.A major challenge of model application is the optimization and calibration of a considerable number of parameters.Sensitivity analysis(SA) has become an effective method to identify the importance of various parameters.In this study,the extended Fourier Amplitude Sensitivity Test(EFAST) approach was used to evaluate the sensitivity of the DSSAT-CERES model output responses of interest to 39 crop genotype parameters and six soil parameters.The outputs for the SA included grain yield and quality(take grain protein content(GPC) as an indicator) at maturity stage,as well as leaf area index,aboveground biomass,and aboveground nitrogen accumulation at the critical process variables.The key results showed that:(1) the influence of parameter bounds on the sensitivity results was slight and less than the impacts from the significance of the parameters themselves;(2) the sensitivity parameters of grain yield and GPC were different,and the sensitivity of the interactions between parameters to GPC was greater than those between the parameters to grain yield;and(3) the sensitivity analyses of some process variables,including leaf area index,aboveground biomass,and aboveground nitrogen accumulation,should be performed differently.Finally,some parameters,which improve the model’s structure and the accuracy of the process simulation,should not be ignored when maturity output as an objective variable is studied.