Induced Resistance of Tomato against Gray Mold (Botrytis cinerea) by Salicylic Acid (SA)

[ Objective ] The paper was to explore the induced resistance of tomato against gray mold （Botrytis cinerea） by salicylic acid. [ Method ] SA was used as an inducer to treat tomato seedlings, the effects of SA on mycelial diameter and spore germination of B. cinerea were studied, and the changes of 4 defense enzyme activities containing catalase （CAT）, peroxidase （POD）, polyphenol oxidase （PPO） and phenylalanine ammonia lyase （PAL）, as well as malondialdehyde （MDA） content during the production process of induced resistance were also measured. [ Result] SA had no inhibitory effect against spore germination and myce- lial growth of B. cinerea within the concentration range, and the relative induced effect had different degrees of improvement after treatment. The induced effect was the best as B. cinerea was challenged to inoculate at the third day after using 150 mg/L SA in tomato plants, and the duration of resistance was 10 -15 d. After treated by SA, CAT, POD, PPO and PAL first increased and then decreased in systemic induced resistance against B. cinerea, which were significantly higher than control. Meanwhile, MDA content showed ascendant trend in wavy line form. [ Conclusion ] The use of SA within a certain concentration range is safe; CAT, POD, PPO and PAL activities have positive correlation with induced resistance against B. cinerea, the increase of MDA content also has close relationship with the imvrovement of disease resistance.

Investigation of Gray Mold of Protected Tomato and Resistance Identification of Variety in Shihezi Area,Xinjiang

[Objective] The paper was to understand the occurrence of gray mold of protected tomato and resistance of introduced tomato varieties to gray mold in Shihezi area. [Method] The occurrence status of gray mold in protected tomato in Shawan County, Manas County, the experimental field of Shihezi University and Shihezi Huayu base were investigated during the occurrence period of tomato gray mold, and the resistance of intro-duced variety against tomato gray mold was identified at seedling stage through indoor artificial inoculation method. [Result] The diseased leaf rate of tomato gray mold was 17.52%-100%, the diseased fruit rate was 3.1%-55.69%, and the disease index was 15.98-100. In resistance identifica-tion of different tomato varieties, the disease indexes of Kenfan25, Shifan28, Shifan15, KENFAN-3, KENFAN-5, Shifan35, KENFAN-2 and Shifan-9 were 1.03, 3.1, 1.17, 4.3, 5.32, 5.12, 4.32 and 4.11, respectively. From September to March of the following year, tomato gray mold occurred and was prevalent in greenhouses in Shihezi area. [Conclusion] Shifan28, Kenfan25 and Shifan15 showed resistance to tomato gray mold, while the other five varieties were susceptible to the disease.

Study on Antifungal Activity of Some Medicinal Plants against Gray Mold(Botrytis cinerea)

In order to investigate antifungnl effect of medicinal plant extracts on Botrytis cinerea, the antifungal activity of ethanol extracts of 8 medicinal plants in- eluding Ginkgo biloba against Botrytis cinerea was determined by growth rate method. The results showed that Ginkgo biloba and Ginnamomum cassia extracts had significant inhibition effects on B. cinema mycelium growth （P 〈 0.05）, with inhibition rates over 70% and ECho values of 0.027 mg/ml and 0.039 mg/ml, re- spectively, significantly different from the antifungal effects of other kinds of medicinal plants ; and P. ternata and A. tsaoko extracts showed the second highest in- hibition effects, with ECho values of 0. 011 9 and 0. 039 3 mg/ml, respectively. Further experimental application could be carried out.

Field Control Effects of Four Biopesticides on Grape Gray Mold

[Objectives]The paper was to search for biopesticides with good control effect on grape gray mold.[Methods]A randomized field trial was carried out to compare the control effects of 100 million CFU/g Trichoderma harzianum WDG,200 million spores/g Trichoderma WP,24% validamycin AS,0.3% matrine AS on grape gray mold.[Results]Of the 4 biopesticides,200 million spores/g Trichoderma WP had the highest control effect on grape gray mold,with the control effect of 73.38% at 7 d post the second application,significantly higher than those of 100 million CFU/g T.harzianum WG and 0.3% matrine AS;the control effect was 75.77% at 14 d post the second application,significantly higher than those of other 3 biopesticides.[Conclusions]The 200 million spores/g Trichoderma WP shows a good control effect on grape gray mold with a long-lasting control effect,so it is more is for the control of summer black grape gray mold in Jianshui County.

Determination on Microclimatic Conditions at Vines upon Development on Gray Mold (<i>Botrytis cinerea</i>)

One of the most important plant diseases in viticulture is gray mold caused by Botrytis cinerea Pers. Fr., the anamorph of an ascomycete fungus (Botryotinia fuckeliana Whetzel). Locality Smilica, Kavadarci, Republic of North Macedonia, was the place where experimental fields with white varieties Smedervka and Zilavka were continuously observed. Working hypothesis was to follow development of the disease after increasing glucose over 11% until the time of the grape harvest, and microclimate was monitored at the same time. In both white varieties Smederevka and Zilavka on the control variants weren’t used botricide treatments to distinguish between the variants that were conventionally treated against B. cinerea. The aim of the research was to determine how microclimatic conditions affect the development of B. cinerea and consequently to create forecasting model for gray mold. The forecasting model for B. cinerea is based on relationship between temperature and humidity in the vines’ canopies. The aim of the research is to prevent development of B. cinerea and consequently reduce the number of chemical treatments.

Plant immune inducer ZNC promotes rutin accumulation and enhances resistance to Botrytis cinerea in tomato

Gray mold is a destructive disease caused by Botrytis cinerea,a pervasive plant pathogen,which poses a threat to both tomato growth and postharvest storage.The utilization of induced resistance presents a potential strategy for combating plant pathogenic attacks.ZNC(zhinengcong),an extract derived from the endophytic fungus Paecilo-myces variotii,has been discovered to play a vital role in preventing diverse forms of bacterial infections.Nevertheless,the precise mechanism behind its ability to enhance tomato resistance to fungi remains unclear.In this study,we found that the exogenous spraying of ZNC could significantly improve the resistance of tomato plants to B.cinerea.The results of both the metabolomic analysis and high-performance liquid chromatography(HPLC)demonstrated that tomato plants responded to ZNC treatment by accumulating high levels of rutin.Additional transcriptome analysis uncovered that rutin enhances tomato resistance possible by initiating the generation of reactive oxygen species(ROS)and phosphorylation of mitogen-activated protein kinases(MPKs)related genes expression during the initial phase of invasion by B.cinerea.In addition,we also found that rutin might activate plant immunity by eliciting ethylene(ET)and jasmonic acid(JA)-mediated pathways.Therefore,plant immune inducer ZNC and rutin has bright application prospects and high utilization value to control gray mold.